全文获取类型
收费全文 | 70544篇 |
免费 | 3423篇 |
国内免费 | 7851篇 |
专业分类
林业 | 9010篇 |
农学 | 11873篇 |
基础科学 | 6778篇 |
12159篇 | |
综合类 | 19625篇 |
农作物 | 3580篇 |
水产渔业 | 2554篇 |
畜牧兽医 | 9539篇 |
园艺 | 2156篇 |
植物保护 | 4544篇 |
出版年
2024年 | 136篇 |
2023年 | 628篇 |
2022年 | 1716篇 |
2021年 | 2599篇 |
2020年 | 2450篇 |
2019年 | 2381篇 |
2018年 | 1622篇 |
2017年 | 2312篇 |
2016年 | 2371篇 |
2015年 | 3105篇 |
2014年 | 2939篇 |
2013年 | 3687篇 |
2012年 | 4277篇 |
2011年 | 4772篇 |
2010年 | 4517篇 |
2009年 | 4282篇 |
2008年 | 4157篇 |
2007年 | 4176篇 |
2006年 | 4043篇 |
2005年 | 3577篇 |
2004年 | 1725篇 |
2003年 | 1534篇 |
2002年 | 1153篇 |
2001年 | 1321篇 |
2000年 | 1574篇 |
1999年 | 1870篇 |
1998年 | 1893篇 |
1997年 | 1574篇 |
1996年 | 1432篇 |
1995年 | 1354篇 |
1994年 | 1183篇 |
1993年 | 1127篇 |
1992年 | 947篇 |
1991年 | 796篇 |
1990年 | 677篇 |
1989年 | 581篇 |
1988年 | 445篇 |
1987年 | 265篇 |
1986年 | 168篇 |
1985年 | 82篇 |
1984年 | 73篇 |
1983年 | 65篇 |
1982年 | 51篇 |
1981年 | 39篇 |
1980年 | 29篇 |
1979年 | 20篇 |
1978年 | 9篇 |
1965年 | 12篇 |
1964年 | 14篇 |
1963年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 765 毫秒
21.
1988年出生的陈将,是安徽省蚌埠市五河县农机大户,投身农机作业服务已有7年多了,算得上是"老农机"了。如今拥有5台约翰迪尔进口青贮饲料收获机,以及其他农机具产品,总资产1 000多万元。没见到陈将之前,很难想象这么年轻的一位小伙,竟有如此的实力和魄力,在青贮机械化收获事业上越做越大,成了远近闻名的农机大户,并且是迪尔公司的"铁粉"。 相似文献
22.
23.
24.
为了研究不同小麦品种萌发期的耐盐性,为小麦萌发期耐盐性鉴定提供快速、准确的鉴定方法和理论依据。本研究以40个冬小麦品种为试验材料,通过主成分分析法(PCA)与神经网络自组织映射(SOM)聚类分析法,研究盐胁迫对40个小麦品种萌发期各项形态指标的影响。主成分分析法结果表明,发芽势、发芽率、最长根长、第一片叶长及胚芽鞘长在PCA下载荷量最大;根据SOM聚类分析结果,‘德抗961’等4个小麦品种属于高度耐盐品种,‘济麦22’等14个小麦品种属于耐盐性品种,‘济麦20’等16个品种属于中等耐盐性品种,‘鲁麦21’等6个小麦品种属于盐敏感性品种。发芽势、发芽率、最长根长、第一片叶长、胚芽鞘长能够作为萌发期小麦耐盐性筛选的主要评价指标,并且结合主成分、SOM聚类分析方法进行小麦萌发期耐盐性鉴定更为科学、方便。 相似文献
25.
Shi-Zhi Wang Guang-Xin E Yan Zeng Yan-Guo Han Yong-Fu Huang Ri-Su Na 《Reproduction in domestic animals》2021,56(6):936-941
The aim of this study was to analyse the association between single-nucleotide polymorphisms within INHA and ACVR2B and litter size in Dazu black goats. In total, twenty-two SNPs were genotyped in 190 individuals by SNaPshot and resequencing. The results showed that three SNPs (SNP_1, SNP_12 and SNP_13 in this study) were detected to have significant additive genetic effect on the recorded goat litter size (p < .05). The SNP_1 (NC_030809.1), a non-synonymous substitution of G for T at chr2-g. 28314990 in the exon 2 of INHA gene (NM_001285606.1), resulted in homozygote 2 (HOM2) contributed 0.25 and heterozygote (HET) contributed 0.12 larger litter than homozygote 1 (HOM1). Meanwhile, SNP_12 (Chr22-g. 11721225 A > T) and SNP_13 (Chr22-g. 11721227 A > C) (NC_030829.1) simultaneously mutated at the first and third position of a triplet AAA (lysine, K) in the exon 4 of ACVR2B gene (XM_018066623.1) had estimated genetic effects of HOM1 (0.00) and HOM2 (0.03) larger than HET (−0.12). In conclusion, one SNPs (chr2-g. 28314990 T > G) within the exon 2 of INHA and two SNPs (Chr22-g. 11721225 A > T and Chr22-g. 11721227 A > C) i n the exon 4 of ACVR2B gene were highly recommended as candidate markers of litter size in Dazu black goats. A large-scale association study to assess the impact of these variants on litter size is still necessary. 相似文献
26.
Wang Zhun Zhang Hengheng Dong Qiang Gui Huiping Wang Xiangru Pang Nianchang Li Yongnian Niu Jing Jin Dingsha Wang Sujie Zhang Xiling Song Meizhen 《棉花学报》2021,32(6):538-551
[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L-1) and high (5 mmol·L-1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient. 相似文献
27.
l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis. 相似文献
28.
The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy. 相似文献
29.
30.
为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。 相似文献