兔健宝对感染球虫病家兔血液学值的影响

本试验探讨了兔健宝对人工感染球虫病家兔的部分血液学值的影响。结果表明：红细胞及白细胞总数、嗜中性白细胞分别于感染后５～２０天、１０～２５天及５～３０天显著低于对照组（Ｐ＜０．０５）;而淋巴细胞百分率和Ｔ细胞阳转率于感染后５～３０天、１０～３０天显著高于对照组（Ｐ＜０．０５）;嗜酸性白细胞、嗜碱性白细胞和单核细胞无明显差异。由此提示：兔健宝具有减轻家兔感染球虫后的肠道出血,调节机体非特异性免疫应答反应和提高机体特异性免疫应答的活性。     

中国兽医生物安全实验室建设与管理

1 生物安全(biosafety) 　　1.1 生物安全的定义 　　生物安全就是“生命安全“.对于兽医工作者来说,就是防止动物病原体进入动物体内或存留在动物所在的地方,防止动物病原体的传播和扩散.它涉及多项措施,旨在防止动物病原体的感染、传播与流行,防止对人类造成危害.……     

利用16S rRNA全长序列鉴定植物乳杆菌

从标明含有嗜酸乳杆菌的活菌胶囊中分离到一株乳杆菌Lal菌株，利用16S rRNA全序列分析法和传统分类法对Lal菌株进行分类鉴定表明：Lal菌株属于植物乳杆菌种新株系，该菌株现在已经被中国科学院北京微生物所菌种保藏中心收录保存，编号为ASl．2986。     

基于LF-NMR及MRI 的2种速生材干燥过程中水分分布状态研究

为了解太阳能干燥过程中速生材内部水分状态及迁移规律,采用低场核磁共振及核磁共振成像检测桉、杨木干燥过程中水分分布、含量变化及迁移过程,借助扫描电镜在微观层面进行分析。结果表明：核磁共振信号强度与称重法所测含水率相关性系数高达0.99以上,因此低场核磁共振可准确测定木材中的水分含量;桉木和杨木饱水试样的核磁共振信号中均存在3种峰,桉木弛豫时间分别为1.32、32.75、403.70 ms,杨木为2.66、32.75、352.12 ms,对应着结合水及两种状态的自由水;随着干燥进行,不同状态水分的横向弛豫时间逐渐减小,木材对水分的束缚增加。当桉木含水率为27.28%,杨木含水率为35.71%左右时,水分散失转为以结合水为主。通过核磁共振成像中氢质子密度的不同可对水分含量进行区分,进而可观测干燥过程中木材内部水分移动状态。因此,通过低场核磁共振及核磁共振成像可研究速生材干燥过程中的内部水分状态及分布。     

白色金针菇工厂化生产技术与装备体系研究

白色金针菇工厂化生产使用规范化的硬件设施,采用标准化的生产流程和层层把关的有效管理,生产受农业环境影响小,确保了产品的产量和品质。白色金针菇工厂化生产流程为:菌种选育与培养基(料)优化(包括基料选配、搅拌、装袋合灭菌等)→规范化生产(包括接种、培养、搔菌、出菇蕾、驯养、生长及采收等环节)与病虫害综合防治技术→商品菇分级包装。     

Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

N2O production in the organic and mineral horizons of soil had different responses to increasing temperature

Journal of Soils and Sediments - The responses of N2O emission to increasing temperature in different soil horizons are not clearly understood yet. Here, we investigated the effects of increasing...     

香菇原生质体工程菌株的构建研究——I.香菇原生质体的分离与再生行为

本研究通过电镜观察与酶解分析确定香菇(Lentinula edodes)菌丝细胞壁组成.并进行了双核菌丝原生质体的分离和培养.研究发现:酶解过程中,菌丝原生质体一般只从菌丝顶端等新生部位释放出来.合适的渗透剂、菌龄和酶解温度是原生质体得率的重要保证;原生质体再生菌丝过程是一个间歇性的依赖于原生质团涌动流向的过程,再生菌丝的形成经历了原生质体形成突起-哑铃状-分枝状等不同形态类型的再生阶段;再生菌丝体在形态上没有观察到锁状联合,栽培后未能结实,初步判断为单核菌株.     

Greenhouse gas emissions in response to straw incorporation,water management and their interaction in a paddy field in subtropical central China

A field experiment was conducted to study the effects of combination of straw incorporation and water management on fluxes of CH₄, N₂O and soil heterotrophic respiration (Rh) in a paddy field in subtropical central China by using a static opaque chamber/gas chromatography method. Four treatments were set up: two rice straw incorporation rates at 0 (S1) and 6 (S2) t ha^?1 combined with two water managements of intermittent irrigation (W1, with mid-season drainage) and continuous flooding (W2, without mid-season drainage). The cumulative seasonal CH₄ emissions for the treatments of S1W2, S2W1 and S2W2 increased significantly by 1.84, 5.47 and 6.63 times, respectively, while seasonal N₂O emissions decreased by 0.67, 0.29 and 1.21 times, respectively, as compared to S1W1 treatment. The significant increase in the cumulative Rh for the treatments S1W1, S2W1 and S2W2 were 0.54, 1.35 and 0.52 times, respectively, in comparison with S1W2. On a seasonal basis, both the CO₂-equivalents (CO₂e) and yield-scaled CO₂e (GHGI) of CH₄ and N₂O emissions increased with straw incorporation and continuous flooding, following the order: S2W2>S2W1>S1W2>S1W1. Thus, the practices of in season straw incorporation should be discouraged, while mid-season drainage is recommended in paddy rice production from a point view of reducing greenhouse gas emissions.     

Molecular cloning and functional analysis of duck Toll-like receptor 5

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-κB-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog.