The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.

Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations.     

青海省草地畜牧业可持续发展对策

概括目前青海省草地畜牧业经营状况 ,探讨草地不断退化、畜牧业经营效益居后的原因 ;结合当地气候、地理和资源特征提出了一些可持续发展举措 ,为提高草地畜牧业的经济效益和可持续发展能力提供一些参考     

Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.     

Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.     

浅谈我省地方畜禽品种资源保护及开发利用

近年来，我省先后从国内外引进了20多个鸡种，10多个牛种、羊种和猪种等，因其高效而占领了我省市场。同时存在着盲目引种，品种多乱、杂等问题。伴随着少数高产畜禽品种的普及推广，使各地固有的许多品种资源迅速减少或消失。因此，针对陕西畜禽品种资源的现状问题，并结合近年来畜牧业生产中的实际状况，特就如何搞好地方畜禽品种资源的保护及开发利用提出建议与对策。     

轻型木结构建筑覆板的集中静载性能

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.     

奎屯地区气象要素对玉米播种及苗期的影响

奎屯地区农七师位于天山北麓,准噶尔盆地的西南缘,总面积5986km2,其中作物种植面积120万亩,随着农七师产业结构的调整,2003年实施了“万头澳牛引进工程”,养牛业的发展使农七师畜牧业对玉米的需求量增加,尤其是澳牛养殖业对青贮玉米需求量更大,因此,研究分析气象要素对玉米播种及苗期的影响是重要工作之一。     

日本栗大面积引种开发技术

山东省日照市引种日本栗大面积开发已历时10 a,在观测综合适应性、筛选优良品种组合、探索栽培技术等方面,积累了成熟经验。针对选择立地条件、发展早中晚熟品种比例、栽培技术规范化等方面存在的问题,提出了明确的对策。并对各地日本栗引种误区,阐明了新的见解。     

我国农村信息化建设存在的问题及对策研究

农村信息化建设是国家信息化战略的重要组成部分，是实现小康社会的重要途径。我国的农村信息化建设取得了初步的成绩，但还存在许多问题，针对这些问题本文提出了具体的应对策略。