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71.
Endosperm and aleurone cell structure in barley and wheat as studied by optical and Raman microscopy
Anna-Stiina Jääskeläinen Ulla Holopainen-Mantila Tarja Tamminen Tapani Vuorinen 《Journal of Cereal Science》2013
Grain ultrastructure is of utmost importance when designing grain processing procedures in the food industry. In this study, wheat and barley grain components were localised using optical and Raman microscopy. The optical microscopic analyses were performed using several selective stains to localise β-glucan, protein and starch or autofluorescence to image the ferulic acid and other fluorescing substances. Alternatively, Raman microscopy was applied to localise the grain components without any need for preceding staining or other sample pretreatment. Both methods provided consistent information on the grain structures, illustrating the distribution of polysaccharides, aromatics and protein in endosperm and aleurone layers. In aleurone layers of both barley and wheat, a distinct difference between the anticlinal and periclinal cell walls was observed. The anticlinal cell walls were enriched with aromatic substances which were present in remarkably lower concentrations in the inner periclinal cell walls but for barley, an even higher concentration in the outer periclinal cell wall was observed. In addition, Raman spectroscopy illustrated the detailed distribution of substances across the aleurone cell walls: β-glucan was adjacent to proteins and it was deficient in the middle lamella whereas arabinoxylan was enriched in the outer cell wall layers and middle lamella. 相似文献
72.
73.
Bouatia-Naji N Rocheleau G Van Lommel L Lemaire K Schuit F Cavalcanti-Proença C Marchand M Hartikainen AL Sovio U De Graeve F Rung J Vaxillaire M Tichet J Marre M Balkau B Weill J Elliott P Jarvelin MR Meyre D Polychronakos C Dina C Sladek R Froguel P 《Science (New York, N.Y.)》2008,320(5879):1085-1088
Several studies have shown that healthy individuals with fasting plasma glucose (FPG) levels at the high end of the normal range have an increased risk of mortality. To identify genetic determinants that contribute to interindividual variation in FPG, we tested 392,935 single-nucleotide polymorphisms (SNPs) in 654 normoglycemic participants for association with FPG, and we replicated the most strongly associated SNP (rs560887, P = 4 x 10(-7)) in 9353 participants. SNP rs560887 maps to intron 3 of the G6PC2 gene, which encodes glucose-6-phosphatase catalytic subunit-related protein (also known as IGRP), a protein selectively expressed in pancreatic islets. This SNP was associated with FPG (linear regression coefficient beta = -0.06 millimoles per liter per A allele, combined P = 4 x 10(-23)) and with pancreatic beta cell function (Homa-B model, combined P = 3 x 10(-13)) in three populations; however, it was not associated with type 2 diabetes risk. We speculate that G6PC2 regulates FPG by modulating the set point for glucose-stimulated insulin secretion in pancreatic beta cells. 相似文献
74.
Per Gunnar Fjelldal Ulla Nordgarden Tom Hansen 《Aquaculture (Amsterdam, Netherlands)》2007,270(1-4):231-239
This study investigated the individual variation in vertebral morphology and mineral content (as % of bone dry weight) in rapid growing underyearling smolt (n = 33) twelve weeks after transfer to seawater. The smolt, which were reared in triplicate tanks, had a large individual variation in vertebral bone mineral content (Tank A: 36.2% min, 45.2% max, 39.6% mean. Tank B: 32.6% min, 46.6% max, 40.0% mean. Tank C: 38.5% min, 47.9% max, 42.4% mean), and were subdivided into two groups based on mean within each tank: high mineral content (HMC, above tank mean, n = 15) and low mineral content (LMC, below tank mean, n = 18). Vertebrae of smolt with a low mineral content were more compressed in the anterior–posterior direction (HMC vertebral length/dorso-ventral diameter ratio 0.83, LMC 0.80). This was related to a change in the growth direction of the vertebral cone. This change occurred around transfer to seawater and was characterised by an increased angle between the wall of the cone of the vertebrae and the cranial–caudal axis, and gave the vertebrae a compressed appearance. Smolt with a high mineral content were significantly longer (HMC 28.1 cm, LMC 26.5 cm) and heavier (HMC 270 g, LMC 210 g) twelve weeks after transfer to seawater. 相似文献
75.
Ulla Nordgarden Tom Hansen Gro-Ingunn Hemre Anne Sundby Bjrn Thrandur Bjrnsson 《Aquaculture (Amsterdam, Netherlands)》2005,250(3-4):862-871
Plasma growth hormone (GH), insulin-like growth factor-I (IGF-I), and insulin were measured in two groups of Salmo salar L. during a one-year study. The fish were reared under either a simulated natural photoperiod (SNP) from January to December or a regime of continuous light from January to June, followed by SNP until December (LL/SNP). Plasma GH levels during spring were low, and lower in the LL/SNP fish (< 0.9 ng ml− 1) than in the SNP fish (> 1.9 ng ml− 1), although the LL/SNP grew better (0.8% per day) than the SNP fish (0.5% per day). Plasma IGF-I levels increased transiently from January (64.7 ng ml− 1) to maximum in late September in the LL/SNP (85.8 ng ml− 1) and in November in the SNP group (87.3 ng ml− 1). The ratio GH:IGF-I was lower in the LL/SNP group during spring when this group grew better than the SNP group. 相似文献