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排序方式: 共有173条查询结果,搜索用时 15 毫秒
171.
Joye SB Leifer I MacDonald IR Chanton JP Meile CD Teske AP Kostka JE Chistoserdova L Coffin R Hollander D Kastner M Montoya JP Rehder G Solomon E Treude T Villareal TA 《Science (New York, N.Y.)》2011,332(6033):1033; author reply 1033
Kessler et al. (Reports, 21 January 2011, p. 312) reported that methane released from the 2010 Deepwater Horizon blowout, approximately 40% of the total hydrocarbon discharge, was consumed quantitatively by methanotrophic bacteria in Gulf of Mexico deep waters over a 4-month period. We find the evidence explicitly linking observed oxygen anomalies to methane consumption ambiguous and extension of these observations to hydrate-derived methane climate forcing premature. 相似文献
172.
Lee J Natarajan M Nashine VC Socolich M Vo T Russ WP Benkovic SJ Ranganathan R 《Science (New York, N.Y.)》2008,322(5900):438-442
Statistical analyses of protein families reveal networks of coevolving amino acids that functionally link distantly positioned functional surfaces. Such linkages suggest a concept for engineering allosteric control into proteins: The intramolecular networks of two proteins could be joined across their surface sites such that the activity of one protein might control the activity of the other. We tested this idea by creating PAS-DHFR, a designed chimeric protein that connects a light-sensing signaling domain from a plant member of the Per/Arnt/Sim (PAS) family of proteins with Escherichia coli dihydrofolate reductase (DHFR). With no optimization, PAS-DHFR exhibited light-dependent catalytic activity that depended on the site of connection and on known signaling mechanisms in both proteins. PAS-DHFR serves as a proof of concept for engineering regulatory activities into proteins through interface design at conserved allosteric sites. 相似文献
173.