Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain

Complementary DNA clones, encoding the LH-hCG (luteinizing hormone-human choriogonadotropic hormone) receptor were isolated by screening a lambda gt11 library with monoclonal antibodies. The primary structure of the protein was deduced from the DNA sequence analysis; the protein contains 696 amino acids with a putative signal peptide of 27 amino acids. Hydropathy analysis suggests the existence of seven transmembrane domains that show homology with the corresponding regions of other G protein-coupled receptors. Three other types of clones corresponding to shorter proteins were observed, in which the putative transmembrane domain was absent. These probably arose through alternative splicing. RNA blot analysis showed similar patterns in testis and ovary with a major RNA of 4700 nucleotides and several minor species. The messenger RNA was expressed in COS-7 cells, yielding a protein that bound hCG with the same affinity as the testicular receptor.     

The challenge and status of IWRM in four river basins in Europe and Asia

This paper assesses the implementation of four selected IWRM principles in four very different river basins in Europe and Asia. The four principles relate to all the different aspects of sustainable development—environmental, social, economic and institutional—as well as the factor that is particularly crucial in many countries of the South: implementation capacity. The paper is based on the work performed in the EC-funded STRIVER project, “Strategy and methodology for improved IWRM—An integrated interdisciplinary assessment in four twinning river basins”. The four basins—Tungabhadra and Sesan (in Asia), and Tagus and Glomma (in Europe) exemplify very different problems and challenges with regard to IWRM: different levels of socio-economic development and very varying problems with regard to water quality and availability. The paper shows that the implementation of IWRM is at a fairly early stage in all the four STRIVER basins; and that successful implementation of water resources is dependent not only on the existence of relevant policies, but also the degree to which laws and policies are in fact implemented.     

Selection based on morphological features of porcine embryos produced by in vitro fertilization: Timing of early cleavages and the effect of polyspermy

The aim of this study was to examine whether a morphological approach is efficient for selecting high‐quality porcine embryos produced by in vitro fertilization (IVF) under high polyspermy conditions. Frozen‐thawed Meishan epididymal spermatozoa showing moderate and high polyspermy were subjected to IVF (1 × 10⁵ sperms/ml). Under conditions of moderate polyspermy, 4‐cell embryos selected at 48 hr after IVF (single selection) and 8‐cell embryos selected at 79 hr after IVF from the collected 4‐cell embryos (double selection) showed high developmental competence. Likewise, 4‐ and 8‐cell embryos produced by IVF under high polyspermy conditions also showed high competence for development to blastocysts. However, blastocysts derived from high polyspermy conditions had significantly fewer cells than those produced under moderate polyspermy conditions. Furthermore, the frequency of nuclear and chromosomal abnormalities in 4‐ and 8‐cell embryos produced under conditions of high polyspermy was significantly (p < .05) higher in comparison to moderate polyspermy conditions. These findings suggest that although high polyspermy affects the frequency of nuclear and chromosomal anomalies in porcine IVF embryos, subsequent selection based on morphological features of 4‐ and 8‐cell embryos even under high polyspermy conditions, could be an alternative option for selecting porcine IVF embryos with high development ability.     

Prevalence of Neospora caninum and bovine viral diarrhoea virus in dairy cows in Southern Vietnam

The main purpose of this study was to investigate the prevalence of Neospora caninum and bovine viral diarrhoea virus (BVDV) in some dairy herds in Southern Vietnam, and to ascertain whether there were differences in seroprevalences between herds with imported and locally bred cows. Serum samples collected on five state farms and 97 smallholder herds were analysed for the presence of antibodies to N. caninum and BVDV. All BVDV antibody-negative sera were further tested by antigen-ELISA in order to identify persistently infected individuals. The N. caninum prevalence varied between 16% and 53% in the state herds, and was higher in the four herds that had imported cows than in the herd that only had locally bred cows. Nineteen percent of the samples collected on smallholder farms, which all had only locally bred cows, had antibodies to N. caninum. The BVDV seroprevalence varied between 58% and 93% on the state farms. In smallholder herds, the prevalence of BVDV among the sampled cows was 18% and even lower on the state farms. Despite the high seroprevalence for BVDV in the state herds, no persistently BVDV infected cows were found. Given the high prevalence for Neospora and BVDV among herds with imported cows, it seems advisable to test for both infections before cattle are imported into the country.     

In vitro production of porcine embryos: current status,future perspectives and alternative applications

The pig is considered to be a suitable source of cells and organs for xenotransplants, as well as a transgenic animal to produce specific proteins, given the biological similarities it shares with human beings. However, the in vitro embryo production system in pigs is inefficient compared with those in other mammals, such as cattle or mice. Although numerous modifications have been applied to improve the efficiency of in vitro embryo production systems in pigs, not much progress has been made to overcome the problem of polyspermy, and low developmental ability due to insufficient cytoplasmic abilities of in vitro matured oocytes and improper culture conditions for the in vitro produced embryos. Recent achievements, such as the establishment of chemically defined medium and utilization of ‘zona hardening’ technique, have gained some success. However, further research for the reduction of polyspermy and detrimental effects of the culture systems in pigs is still needed.     

Factors constraining and enabling agroforestry adoption in Viet Nam: a multi-level policy analysis

Agroforestry is known to have multiple economic and environmental benefits. Despite this, the adoption of agroforestry in Viet Nam is limited both in extent and diversity of components and practices. Our study identified gaps for agroforestry adoption in current policies and policy implementation. National policy and provincial instructions and decisions were reviewed and discussed during ten stakeholders’ consultation workshops. The review and workshops concluded that there were no specific national and few provincial specific policies promoting agroforestry. In addition, the segregation of policies into agriculture and forestry, promoted monoculture practices and discouraged the integration of mixed annual and perennial tree species. Completing the forestland allocation process was considered essential for long-term investment on land and providing collateral for loans. More holistic policies, such as a provincial strategy for agroforestry development that would enable flexible integration of agriculture, forestry and livestock were perceived to be more effective and inclusive to poor and non-poor farmers. Specific cross-cutting budget allocation would be necessary for capacity building, upscaling agroforestry models, procurement of high-quality inputs, and establishing post-harvest processing and marketing investments.     

Characteristic features of porcine endogenous retroviruses in Vietnamese native pigs

We aimed to clarify the genomic characteristics of porcine endogenous retroviruses (PERVs) in Vietnamese native pig (VnP) breeds. First, we investigated genetic polymorphisms in β‐ and γ‐like PERVs, and we then measured the copy numbers of infectious γ‐like PERVs (PERV‐A, B, and C). We purified genomic DNA from 15 VnP breeds from 12 regions all over the country and three Western pig breeds as controls, and investigated genetic polymorphisms in all known PERVs, including the beta (β)1–4 and gamma (γ)1–5 groups. PERVs of β1, β2, β3, and γ4 were highly polymorphic with VnP‐specific haplotypes. We did not identify genetic polymorphisms in β4, γ1, or γ2 PERVs. We then applied a real‐time polymerase chain reaction–based method to estimate copy numbers of the gag, pol, and env genes of γ1 PERVs (defined as A, B, and C). VnP breeds showed significantly lower copy number of the PERV genes compared with the Western pig breeds (on average, 16.2 and 35.7 copies, respectively, p < .05). Two VnP breeds showed significantly higher copy number compared with the other VnPs (p < .05). Our results elucidated that VnPs have specific haplotypes and a low copy number of PERV genes.     

Farm types for beef production and their economic success in a mountainous province of northern Vietnam

The objective of this study was to compare the management and economic success of beef production by three types of farm in northwestern Vietnam. The potential of household farms to supply beef for the market and their competition with large farms were examined.The fieldwork was done in 2007 on 73 farms consisting of 58 small mixed farms (small farms), 10 medium mixed farms (medium farms) and 5 specialised large-scale beef farms (large farms) in Son La province. The three types of farm differed in ethnicity (Thai, H’mong, and Kinh), remoteness (lowland, highland), production objectives (subsistence, market output), degree of specialization (mixed farm, specialised beef farm) and integration of production (single farmers, cooperative). Data on biological productivity, inputs and outputs, and the social contribution of cattle production were collected by household and key person interviews, participatory rural appraisal tools and cattle body measurements. Economic values were derived by assessment of market or replacement costs. Quantitative data analysis was done with linear models (PROC GLM) in the SAS software (version 9.1).Lowland small farms had higher costs for cattle production than the highland farms (0.8 Mill. VND head⁻¹ year⁻¹ compared with 0.02 Mill. VND head⁻¹ year⁻¹, respectively). The large farms had high production costs, with an average of 2.5-3.6 Mill. VND head⁻¹ year⁻¹. Cattle brought high benefits of non-cash values to the household farms. The total revenue from cattle was in the range 4.5-11.5 Mill. VND head⁻¹ year⁻¹, which depended on the use of non-market functions of cattle on the household farm. The value of net benefit/kg live weight (LW) of lowland small farms with an average of 39,000 VND/kg LW was significantly higher than that of the medium and small farms in the highlands (26,000 VND/kg LW). However, the small farms kept fewer cattle than the medium farms (average of 2-4 cattle/farm compared with 9 cattle/farm, respectively) because of forage and labour shortages and have no option to further develop cattle production. Keeping larger numbers of cattle based on available natural pasture brought high benefit from stock value as farm liquidity to only the medium farms. This was the most promising type of farm for future development of beef production, given its actual success and the availability of underutilised resources. Large-scale farms suffered high economic losses of 0.3-1.4 Mill. VND cattle⁻¹ year⁻¹, due to the lack of professional management, high feed costs and low animal performance, and showed no potential for developing cattle production.     

A comparison of the effect of dietary fungal phytase and dicalcium phosphate supplementation on growth performances,feed and phosphorus utilization of tra catfish juveniles (Pangasianodon hypophthalmus Sauvage, 1878)

Six isonitrogenous (320 g kg^?1) and isolipidic (60 g kg^?1) diets were formulated with graded levels (0, 5, 10 and 15 g kg^?1) of dicalcium phosphate (DCP) and fungal phytase (750 and 1500 FTU kg^?1 diet). Tra catfish (Pangasianodon hypophthalmus), 9.6 g, were fed the diets for 12 weeks. Each experimental diet was fed to eight replicates of fish to apparent satiation. At the end of the trial, fish fed the diets containing 15 g kg^?1 DCP, 750 and 1500 FTU kg^?1 phytase had higher growth performances, protein efficiency ratio and phosphorus retention than those fed the control diet, 5 g kg^?1 DCP and 10 g kg^?1 DCP diets (P < 0.05). Whole body ash and phosphorus concentration of fish fed the 10 g kg^?1 DCP and 15 g kg^?1 DCP diets were significantly higher than those of fish fed the control diet. Higher apparent digestibility coefficient of phosphorus was observed in fish fed the phytase supplemented diets. The present results indicate that supplementation of phytase at 750 FTU kg^?1 and 1500 FTU kg^?1 improves growth performances, feed and phosphorus utilization. The supplementation can completely replace dicalcium phosphate or other phosphorus sources in tra catfish feed and reduce the phosphorus discharge into environment.     

Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam

This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin and Shiga toxin. The eae gene was found in 34 isolates (9.8%), 23 of which also carried stx genes. The Shiga toxin genes were detected in 177 isolates (51.3%) and the number of strains that carried stx1, stx2 and stx1/stx2 were 46, 73 and 58, respectively. Among 177 Shiga toxin-producing E. coli isolates, 89 carried the ehxA gene and 87 possessed the saa gene. Further characterization of the stx subtypes showed that among 104 stx1-positive isolates, 58 were the stx1c variant and 46 were the stx1 variant. Of the 131 stx2-positive strains, 48 were stx2, 48 were stx2c, 11 were stx2d, 17 were stx2g, and seven were stx2c/stx2g subtypes. The serogroups most prevalent among the 345 isolates were O15, O20, O103 and O157.