全文获取类型
收费全文 | 5520篇 |
免费 | 248篇 |
国内免费 | 4篇 |
专业分类
林业 | 423篇 |
农学 | 183篇 |
基础科学 | 35篇 |
1101篇 | |
综合类 | 877篇 |
农作物 | 231篇 |
水产渔业 | 344篇 |
畜牧兽医 | 2002篇 |
园艺 | 177篇 |
植物保护 | 399篇 |
出版年
2023年 | 33篇 |
2022年 | 54篇 |
2021年 | 84篇 |
2020年 | 135篇 |
2019年 | 126篇 |
2018年 | 135篇 |
2017年 | 121篇 |
2016年 | 130篇 |
2015年 | 110篇 |
2014年 | 131篇 |
2013年 | 259篇 |
2012年 | 303篇 |
2011年 | 382篇 |
2010年 | 220篇 |
2009年 | 168篇 |
2008年 | 310篇 |
2007年 | 317篇 |
2006年 | 291篇 |
2005年 | 289篇 |
2004年 | 264篇 |
2003年 | 271篇 |
2002年 | 256篇 |
2001年 | 64篇 |
2000年 | 71篇 |
1999年 | 82篇 |
1998年 | 56篇 |
1997年 | 53篇 |
1996年 | 55篇 |
1995年 | 39篇 |
1994年 | 47篇 |
1993年 | 49篇 |
1992年 | 39篇 |
1991年 | 46篇 |
1990年 | 46篇 |
1989年 | 42篇 |
1988年 | 38篇 |
1987年 | 39篇 |
1986年 | 54篇 |
1985年 | 32篇 |
1984年 | 43篇 |
1983年 | 35篇 |
1982年 | 36篇 |
1981年 | 27篇 |
1980年 | 20篇 |
1979年 | 23篇 |
1978年 | 27篇 |
1977年 | 26篇 |
1975年 | 20篇 |
1973年 | 24篇 |
1972年 | 20篇 |
排序方式: 共有5772条查询结果,搜索用时 15 毫秒
941.
942.
943.
Hampel V Schwarz B Kempf C Köstlin R Schillinger U Küchenhoff H Fenske N Brill T Hirschberger J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(6):1340-1346
BACKGROUND: Recombinant feline interferon-omega (rFeIFN-omega) was tested as a treatment option for cats with fibrosarcoma to assess safety and feasibility. HYPOTHESIS: Treatment with rFeIFN-omega in cats with fibrosarcoma is safe and feasible. ANIMALS: Twenty domestic cats. METHODS: In an open-labeled uncontrolled clinical trial 12 injections of 1 x 10(6) U/kg rFeIFN-omega were administered over a 5-week period: the 1st through 4th injections were given intratumorally, and the 5th through 12th injections were administered subcutaneously at the tumor excision site. Wide surgical excision of the tumors was carried out after the 4th injection and before the 5th injection of rFeIFN-omega. A Common Terminology Criteria for Adverse Events (CTCAE) analysis was conducted. Flow cytometry of fibrosarcoma cells after incubation with rFeIFN-omega and recombinant feline interferon-gamma was performed to assess the biological effect of rFeIFN-omega. RESULTS: Changes in blood cell count, increases in serum aspartate-amino-transferase activity, serum bilirubin concentration, serum creatinine and serum electrolyte concentrations, weight loss, anorexia, increased body temperature, and reduced general condition were observed but were mostly minor (grade 1 and 2) and self limiting. Eosinophilia (P = .025), neutropenia (P = .021), and weight loss (P < .001) were statistically correlated with rFeIFN-omega-treatment (analysis of parameters before treatment and after 3 injections of rFeIFN-omega). Flow cytometry of 5 unrelated feline fibrosarcoma cell lines showed increased expression of major histocompatibility complex (MHC) class I molecules (P = .026) in response to in vitro incubation with rFeIFN-omega, whereas expression of MHC class II molecules was not affected significantly. CONCLUSIONS AND CLINICAL IMPORTANCE: RFeIFN-omega for the treatment of feline fibrosarcoma is safe, well tolerated, and can be easily performed in practice. To assess the efficacy of the treatment, it should be tested in a placebo-controlled trial. 相似文献
944.
Efficacy of Postoperative Antimicrobial Use for Clean Orthopedic Implant Surgery in Dogs: A Prospective Randomized Study in 100 Consecutive Cases 下载免费PDF全文
945.
Pedersen NC North TW Rigg R Reading C Higgins J Leutenegger C Henderson GL 《Veterinary immunology and immunopathology》2003,94(3-4):133-148
16alpha-Bromo-epiandrosterone (epiBr), a synthetic derivative of the natural hormone dehyroepiandrosterone (DHEA), was evaluated for its effects on feline immunodeficiency virus (FIV) infection in experimental cats. The rationale for this study was based on the ability of DHEA to significantly reduce the mortality to viral infections in mice. DHEA and epiBr also have demonstrable in vitro anti-viral activity for both HIV-1 and FIV. Preliminary pharmacokinetic studies in cats demonstrated that subcutaneously injected epiBr was rapidly absorbed, completely metabolized, and nontoxic. Metabolites were excreted in both urine and feces, with the latter having the most complex pattern of breakdown products. Cats were then divided into four groups; two groups were infected with FIV and two uninfected. Two groups, one infected and one uninfected were treated on 5 consecutive days of weeks 0, 4, 8, 12 and 16 with epiBr. The remaining two groups were mock treated with the drug vehicle alone. Treatment started 1 week prior to infection and extended for 4 weeks after infection. Cats were observed for 20 weeks post-FIV infection. Infected cats had identical decreases in blood neutrophil and lymphocyte counts following, regardless of whether they were treated with epiBr or vehicle alone. The CD4/CD8 T-cell ratio was decreased following FIV exposure, but was significantly more decreased for the epiBr treated animals from week 2 post-infection onward. CD4+ T cells were decreased in FIV-infected cats treated with epiBr compared to their untreated cohort, while CD8+ T cells tended to be higher in treated animals. FIV infected cats that were treated with epiBr had over one-log higher virus loads at week 2 post-infection than non-epiBr treated cohorts. In spite of this enhanced initial viremia, the subsequent levels of virus in the blood were significantly lower in epiBr treated versus untreated animals. EpiBr treated cats had significantly higher FIV-p24 antibody responses than control cats receiving vehicle alone, although primary and secondary antibody responses to a T-cell dependent non-FIV antigen, keyhole limpet hemocyanin (KLH), were unaffected. EpiBr treatment significantly decreased the expected FIV-induced suppression of IL-12 p40 mRNA levels in peripheral blood mononuclear cells (PBMCs) observed at weeks 4, 5, 8, 9 and 16 post-infection, but had no influence on FIV-induced changes in IL-4, IL-6, IL-10, IFN-gamma, MIP-1alpha and RANTES. 相似文献
946.
Kathryn C Gamble Michael M Garner Laura Krause Thomas P Alvarado 《Journal of zoo and wildlife medicine》2004,35(3):361-369
Two adult female rock hyraxes (Procavia capensis) at the Dallas Zoo were confirmed with spontaneous diabetes mellitus from 1997-2000, whereas a third animal with a similar clinical presentation never became hyperglycemic. The pancreas in all three animals showed pancreatic islet fibrosis (PIF). Retrospective examination of medical records for rock hyraxes acquired by this collection or born into it from 1991-2002 identified eight more animals affected with PIE All affected animals, including three males and eight females, were 1-7 yr of age and presented either with vague clinical signs of soft feces and rough hair coat or were acutely moribund or dead. Clinical pathology data was available for seven of the animals before onset of overt clinical signs and revealed inappropriate hyperglycemia in six, as well as elevated serum concentrations of creatine phosphokinase, amylase, and lipase in all seven animals. Pedigree evaluation did not support a familial pattern for PIE Review of the histopathology findings from nine other zoologic collections with rock hyrax deaths during the study period identified six institutions with 12 additional cases genetically unrelated to the incident collection. Histopathology and viral serology did not support an infectious cause. Analysis of serum anti-islet and anti-insulin antibodies did not suggest autoimmune disease, and none of the animals had known exposure to toxic substances. Limited nutritional analyses did not support a nutritional basis for the condition, and the cause for PIF remains unknown. 相似文献
947.
Riggs LM Franck T Moore JN Krunkosky TM Hurley DJ Peroni JF de la Rebière G Serteyn DA 《American journal of veterinary research》2007,68(1):81-86
OBJECTIVE: To compare measurements of myeloperoxidase (MPO) in plasma, laminar tissues, and skin obtained from control horses and horses given black walnut heartwood extract (BWHE). ANIMALS: 22 healthy 5- to 15-year-old horses. PROCEDURES: Horses were randomly assigned to 4 groups as follows: a control group given water (n = 5) and 3 experimental groups given BWHE (17) via nasogastric intubation. Experimental groups consisted of 5, 6, and 6 horses that received BWHE and were euthanatized at 1.5, 3, and 12 hours after intubation, respectively. Control horses were euthanatized at 12 hours after intubation. Plasma samples were obtained hourly for all horses. Laminar tissue and skin from the middle region of the neck were harvested at the time of euthanasia. Plasma and tissue MPO concentrations were determined via an ELISA; tissue MPO activity was measured by use of specific immunologic extraction followed by enzymatic detection. RESULTS: Tissues and plasma of horses receiving BWHE contained significantly higher concentrations of MPO beginning at hour 3. Laminar tissue and skin from horses in experimental groups contained significantly higher MPO activity than tissues from control horses. Concentrations and activities of MPO in skin and laminar tissues were similar over time. CONCLUSIONS AND CLINICAL RELEVANCE: In horses, BWHE administration causes increases in MPO concentration and activity in laminar tissue and skin and the time of increased MPO concentration correlates with emigration of WBCs from the vasculature. These findings support the hypothesis that activation of peripheral WBCs is an early step in the pathogenesis of acute laminitis. 相似文献
948.
最近 ,法国报道全国 80 %的母猪都感染了疥螨寄生虫 ,有 30 %的生长肥育猪舍受到影响。以前也曾有报道表明西班牙和英国的生长肥育猪分别有 2 7.5%和 30 %感染了疥螨 ,其中西班牙有80 %的猪舍发现疥螨 ,英国 67%的猪舍发现疥螨。在德国发现感染疥螨属疥疮 var.suis寄生虫的肥育猪占 36.5%,比利时和丹麦分别为 36%和2 7.5%,其中比利时有 30 %的猪舍发现疥螨感染 ,丹麦则占 40 %。意大利最近报道 ,在体重为2 5kg至屠宰的肥育猪中检测到 1 3%的猪感染了疥螨 ,几乎 58%的肥育猪舍都发现了感染个体。在意大利的屠宰场胴体检测中发现约 62 %的猪由… 相似文献
949.
MA Elzo GC Lamb DD Johnson MG Thomas I Misztal DO Rae CA Martinez JG Wasdin JD Driver 《Journal of animal science》2012,90(8):2488-2497
The objectives of this study were to determine the fraction of additive genetic variance explained by the SNP from the Illumina Bovine3K chip; to compare the ranking of animals evaluated with genomic-polygenic, genomic, and polygenic models; and to assess trends in predicted values from these 3 models for residual feed intake (RFI), daily feed intake (DFI), feed conversion ratio (FCR), and postweaning BW gain (PWG) in a multibreed Angus-Brahman cattle population under subtropical conditions. Data consisted of phenotypes and genotypes from 620 bulls, steers, and heifers ranging from 100% Angus to 100% Brahman. Phenotypes were collected in a GrowSafe automated feeding facility (GrowSafe Systems, Ltd., Airdrie, Alberta, Canada) from 2006 to 2010. Variance components were estimated using single-trait genomic-polygenic mixed models with option VCE (Markov chain Monte Carlo) of the program GS3. Fixed effects were contemporary group (year-pen), age of dam, sex of calf, age of calf, Brahman fraction of calf, and heterozygosity of calf. Random effects were additive SNP, animal polygenic, and residual effects. Genomic predictions were computed using a model without polygenic effects and polygenic predictions with a model that excluded additive SNP effects. Heritabilities were 0.20 for RFI, 0.31 for DFI, 0.21 for FCR, and 0.36 for PWG. The fraction of the additive genetic variance explained by SNP in the Illumina 3K chip was 15% for RFI, 11% for DFI, 25% for FCR, and 15% for PWG. These fractions will likely differ in other multibreed populations. Rank correlations between genomic-polygenic and polygenic predictions were high (0.95 to 0.99; P < 0.0001), whereas those between genomic-polygenic and genomic predictions were low (0.65 to 0.74; P < 0.0001). Genomic-polygenic, genomic, and polygenic predictions for all traits tended to decrease as Brahman fraction increased, indicating that calves with greater Brahman fraction were more efficient but grew more slowly than calves with greater Angus fraction. Predicted SNP values were small for all traits, and those above and below 0.2 SNP SD were in multiple chromosomes, supporting the contention that quantitative traits are determined by large numbers of alleles with small effects located throughout the genome. 相似文献
950.
Thomas Gaiser Michael JudexAttanda Mouinou Igué Heiko PaethClaudia Hiepe 《Agricultural and Forest Meteorology》2011,151(8):1120-1130
In this century climate change is assumed to be the major driver for changes in agricultural systems and crop productivity at the global scale. However, due to spatial differences in cropping systems and in the magnitude of climatic change regional variations of climate change impact are expected. Furthermore, the recent climate projections are highly uncertain for large parts of West Africa. In particular with respect to annual precipitation and variability the projections vary between trends with decreasing precipitation and trends with slightly increasing precipitation within the next decades. On the other hand, the extensive fallow systems in this region suffer from increasing population pressure, which compromises soil fertility restoration. In the Republic of Benin, the demographic projections for the first half of this century indicate a continuous growth of the population with a narrow interval of confidence. Thus, in the absence of an adequate soil fertility management with judicious use of mineral fertilizers, the soil degradation process with decreasing crop yields is expected to continue. The objective of this paper was, therefore, to quantify the regional effect of future population growth on crop yields in West Africa and to compare it with the potential effects of climate change scenarios. Three land use scenarios (L1, L2 and L3) for the Upper Ouémé catchment where derived from different demographic projections combined with assumptions regarding future road networks and legal frameworks for forest protection using the CLUE-S modeling approach. The fallow-cropland ratio decreased in the three scenarios from 0.87 in the year 2000 to 0.66, 0.48 and 0.68 for L1, L2 and L3, respectively in 2050. Based on the projected ratio of fallow and cropland, trends of maize yield for the three land use scenarios were calculated using the EPIC (Environmental Policy Integrated Climate) model coupled with a spatial database. Maize yields followed the decreasing trend of the fallow-cropland ratio and estimated yield reductions amounted to up to 24% in the period 2021-2050. This trend was compared with the impact of the SRES climate scenarios A1B and B1 based on the output of the GCM ECHAM5 downscaled with the REMO model and the A1B scenario output of the GCM HADC3Q0 downscaled with the RCMs SMHIRCA and HADRM3P. The yield reductions due to the projected climate change in the three models accounted for a yield decrease of up to 18% (REMO A1B scenario) in the same period. Taking into account the smaller uncertainties in the scenario assumptions and in the model output of the land use scenarios, it is concluded that, in low input fallow systems in West Africa, land use effects will be at least as important as climate effects within the next decades. 相似文献