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81.
Satoshi?KusudaEmail author Nobuhisa?Koide Hiloshi?Kawamula Tetsuo?Teranishi Jun?-Ichiro?Nakajima Etsuro?Yamaha Katsutoshi?Arai Hiromi?Ohta 《Fisheries Science》2005,71(2):293-298
To develop a suitable cryopreservation diluent for spermatozoa of the endangered Sakhalin taimen Hucho perryi, all possible combinations of cryoprotectants (glycerol, dimethyl sulfoxide [DMSO], methanol) and extenders (fetal bovine
serum [FBS], 300 mM glucose solution [GS], artificial seminal plasma for masu salmon) were examined by observing sperm motility
10 s after thawing. Spermatozoa cryopreserved with diluents such as mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90%
FBS, and 10% methanol plus 90% GS showed the highest motility. The maximal post-thaw motility was observed at 10% among all
concentrations (0, 5, 10, 15 and 20%) of these three cryoprotectants. No significant difference among three diluents was observed
in motility at 10 s. Mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90% FBS, and 10% methanol plus 90% GS are suitable
cryopreservation diluents for Sakhalin taimen spermatozoa. 相似文献
82.
Yoshikatsu Genda Kyoko Sato Osamu Nunomura Tetsuo Hirabayashi Shinya Tsuda 《Journal of General Plant Pathology》2011,77(3):201-208
The location of Pepper mild mottle virus (PMMoV) within seeds as they developed on inoculated seedlings of pepper (Capsicum annuum) was followed over time by detecting the viral coat protein using immunofluorescence microscopy. Seedlings were inoculated
with PMMoV when the flower buds on the first and second branching nodes were in bloom. Fluorescence indicating the presence
of PMMoV was first observed around immature seeds and placentas in the ovaries on the fourth branching node at 20 days post-anthesis
(20 DPA), which corresponded to 39 days post-inoculation (39 DPI). The area with fluorescence gradually expanded from the
placenta into the integument and the parenchyma, and finally reached the tip of the immature seeds by 34 DPA (53 DPI). The
embryo or endosperm beyond the endothelium never fluoresced during the experiment [i.e., ending at 81 DPA (102 DPI)]. For
visualizing viral routes of invasion from seeds into new seedlings, PMMoV-infected C. annuum seeds that were heterozygous for the L
3
tobamovirus-resistance gene were sown in soil at 30°C. After ~2 weeks, the cotyledon developed virally induced necrosis.
These findings shed light on the infection cycle of PMMoV through vertical transmission in C. annuum. 相似文献
83.
84.
de la Rue ML Takano K Brochado JF Costa CV Soares AG Yamano K Yagi K Katoh Y Takahashi K 《Veterinary parasitology》2011,177(1-2):97-103
The Rio Grande do Sul state, in Southern Brazil, is one of the foci of human cystic echinococcosis (CE). The sheep strain (G1) of Echinococcus granulosus and Echinococcus ortleppi (also known as cattle strain G5) have been reported before to infect livestock. However, up to the present, no molecular data are available on isolates of the E. granulosus complex from humans and dogs. The present study analyzed hydatid cysts from 6 CE patients and adult worms from 12 dogs. Sequencing of the mitochondrial cox1 and 12S rRNA genes detected the E. granulosus G1 genotype from four human cases, the G3 genotype (or buffalo strain) from one human case and E. ortleppi from another human case, respectively. Ten of the twelve dogs were found infected with the G1 genotype, and one dog each harbored worms of the G3 genotype and E. ortleppi. Obvious morphological differences were recognized between the G1 and E. ortleppi adult worms from dogs in this region. The buffalo strain (G3) is for the first time reported from South America. 相似文献
85.
Yohei?Terasawa Kanenori?Takata Hisashi?Hirano Kenji?Kato Taihachi?Kawahara Tetsuo?Sasakuma Tsuneo?SasanumaEmail author 《Genetic Resources and Crop Evolution》2011,58(2):283-289
To clarify the genetic properties of the HMW glutenin subunit composition of Asian endemic wheats, SDS–PAGE analysis was conducted
using 1,139 bread wheat accessions that were originally collected in Asia. The samples were divided into six regional groups,
Western Asia, Caucasia, Central Asia, Afghanistan, Southern Asia, and Eastern Asia. The genotype Glu-A1c, Glu-B1b, and Glu-D1a encoding subunits null, 7+8, and 2+12 had an overall frequency of 55.2%. Thus, we conclude that it is the typical genotype
of the HMW glutenin subunits that characterize Asian endemic wheat. The frequency of the typical Asian genotype was relatively
high in the central belt of Asia (Western Asia, Afghanistan, and Eastern Asia) and low in the marginal regions (Caucasia,
Central Asia, and Southern Asia). In Southern Asia, the frequency of Glu-B1i, which encodes subunit 17+18, was the highest at the Glu-B1 locus. In Caucasia and Central Asia, the frequency of Glu-D1d, which encodes subunit 5+10 (which is considered to be the most useful for making bread), was high. The level of genetic
variation, as estimated using the frequencies of the various alleles, was relatively low in the central belt of Asia and high
in the marginal regions. Among the three Glu-1 loci, the highest number of alleles was detected at the Glu-D1 locus. This result was caused by the presence of rare Asian specific alleles at the Glu-D1 locus, in which a newly found allele, Glu-D1bs, encoding subunit 2.1+12 was included. 相似文献
86.
Ishii N Wei M Kakehashi A Doi K Yamano S Inaba M Wanibuchi H 《Journal of toxicologic pathology》2011,24(1):25-36
In the present study, modifying effects of diabetes on carcinogenesis induced in type 2 diabetes mellitus model Zucker diabetic fatty (ZDF) rats were investigated using a multiorgan carcinogenesis bioassay. Our re sults demonstrated enhancement of urinary bladder, colon and liver carcinogenesis in ZDF rats treated with five types of carcinogens (DMBDD). Elevated insulin and leptin and decreased adiponectin levels in the serum may be responsible for the high susceptibility of type 2 diabetes mellitus model rats to carcinogenesis in these organs. Possible mechanisms of increased susceptibility of diabetic rats to bladder carcinogenesis could be activation of the PI3K pathway and suppression of p53 in the urothelium in consequence of the above serum protein alterations. 相似文献
87.
Takeshi YUI Tetsuo OHMACHI Kazuya MATSUDA Minoru OKAMOTO Hiroyuki TANIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):467-473
Chordomas of the tip of the tail in 6 ferrets were examined using histopathological,
histochemical and immunohistochemical procedures. Histopathologically, round neoplastic
cells containing numerous cytoplasmic vacuoles of varying sizes, categorized as
“physaliphorous cells”, were observed in the amorphous eosinophilic or pale basophilic
myxoid stroma. Physaliphorous cells were arranged in lobules and in a “chordoid” or
“cobblestone” manner. The neoplasms were diagnosed as benign chordoma without local
invasion and metastasis. Histochemically, the cytoplasm of small neoplastic cells was
positive for periodic acid-Schiff stain and alcian blue (AB) pH 2.5 and pH 1.0 stains, but
negative for hyaluronidase digestion-AB pH 2.5 stain. All neoplastic cells were strongly
stained with colloidal ion, negative for high iron diamine AB pH 2.5 and toluidine blue pH
2.5 stains, and positive for Mayer’s mucicarmine stain. Immunohistochemistry using
antibodies directed against low-molecular-weight cytokeratins (CK18, CK19 and CK20),
vimentin and mucin core protein (MUC5AC) revealed that neoplastic cells had both
epithelial and mesenchymal elements. The expression of low-molecular-weight cytokeratins
suggests that neoplastic cells acquired the properties of glandular epithelial cells and
produced epithelial mucus. Furthermore, the expression of cytokeratins, vimentin, S100
protein, brachyury and epithelial membrane antigen indicates that the neoplasms were
equivalent to the classic type of human chordoma. Therefore, immunohistochemistry using
these antibodies can be useful for the characterization of ferret chordoma. 相似文献
88.
Comparison of the diuretic effect of furosemide by different methods of administration in healthy dogs
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89.
Harada T 《Journal of circadian rhythms》2004,2(1):4
Background
In a previous study, when adult subjects were exposed to a level of 400 lux light for more than 30 min or a level of 300 lux light for more than 2 hours, salivary melatonin concentration during the night dropped lower than when the subjects were exposed to dim illumination. It was suggested that such light exposure in adolescents or children during the first half of subjective night in normal life might decrease the melatonin level and prevent the falling into sleep. However, there has been no actual study on the effects of light exposure in adolescents. 相似文献90.
Aluminum toxicity and related nutrient deficiencies, such as phosphorus deficiency, are considered to be major environmental constraints on food production world wide (Foy 1983). Toxic effects of Al in cells are characterized by the inhibition of cell division (Morimura et al. 1978) and nuclear activity (Matsumoto et al. 1977). Therefore, a plant cell culture system could be used as a model system for the physiological study of aluminum toxicity in higher plants. For such a study, it is important to initially define the factors affecting cell responses to aluminum toxicity. 相似文献