Phylogenetic and expression analysis of the magnesium transporter family in pear,and functional verification of PbrMGT7 in pear pollen

The magnesium ion (Mg²⁺) is the most abundant divalent cation in living cells, and it plays essential roles in various cellular functions. Mg²⁺ homeostasis in plant cells is crucial for plant growth. The MRS2/MGT family of proteins localise to various membranes, and they function during Mg²⁺ transport in plants, but their functions are largely unknown in fruit trees. In this study, we performed a genome-wide analysis to identify the MGT gene family members in the pear Pyrus bretschneideri. Sixteen MGT genes were identified. Analysis of the activity of pear pollen showed that a low-concentration magnesium ion treatment was beneficial for pollen germination and pollen tube growth. None of the 16 MGT genes were expressed in any unique tissue, including pollen, and their expression in magnesium-treated pollen varied greatly, where PbrMGT7 was the most sensitive and it was tested as a representative. PbrMGT7 could functionally complement a bacterial strain deficient in Mg²⁺, thereby indicating a role in magnesium transport. A co-localisation experiment confirmed that PbrMGT7 was localised in the mitochondria, which suggests that PbrMGT7 could mediate Mg-trafficking between the mitochondria and cytosol. Overall, these results suggest that PbrMGT7 is related to Mg²⁺ homeostasis during pear pollen tube growth.??     

Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut

In order to assess the survival of lactic acid bacteria (LAB) in whole crop maize silage in the gut of dairy cows, one representative silage sample and three different feces samples were collected from dairy cows on three dairy farms in Hua Bei, China and three dairy farms in Kyushu, Japan. The composition of the bacterial community was examined by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Lactobacillus acetotolerans was detected in all bunker‐made maize silage samples, regardless of the dairy farm or sampling region from which they were sourced. A total of eight LAB species were detected in the maize silage samples, of which three (L. acetotolerans, L. pontis and L. casei) appeared to survive digestion. The populations of L. acetotolerans in silage and feces were 10^6–7 and 10^3–4 copies/g, respectively, indicating that, even for the LAB species showing potential survival in the gut, competition in this niche may be harsh and the population may substantially decrease during the digestion process. It may be difficult for silage LAB to survive in the gut of silage‐fed dairy cows, because marked decrease in population can take place during the digestion process, even for surviving species.     

Transplantation Therapy of Cats with Chronic Renal Failure

Dialysis therapy is not a radical cure of chronic renal failure in cats,while renal transplantation is the cure method.Renal transplanta-tion requires rigorous examination of donor and receptor,and the procedure is strictly sterile.The main points of renal transplantation are:renal artery and vein sutured to abdominal aorta and posterior vena cava,respectively;ureter sutured to bladder;minimizing the time of thermal ischemia.Cats should be given immunosuppressant before the operation,and the drug should be administered throughout the life.Various indicators should be monitored regularly to prevent the occurrence of immunological rejection.     

优质香型超高产两系中籼组合两优6326 特征特性及应用

推广优质香型超高产两系杂交中籼组合两优6326.介绍两优6326的选育经过、产量表现、特征特性和栽培、繁殖制种技术.两优6326系宣城市农科所用自主选育的优质籼型光温敏核不育系"宣69S"为母本、籼粳交优质淡香型恢复系"中籼WH26"为父本,配组而成的优质香型超高产两系杂交中籼组合.2004年3月通过安徽省农作物品种审定,2007年通过国审(国审稻2007013).两优6326各方面的表现优异,配套高产无公害栽培技术、优质高产繁殖制种技术有助于成果转化.现累计示范、推广面积逾45万hm2.该品种适宜在长江中下游南方稻区作优质一季稻种植,有利于优化粮食品质、促进农民增收.     

湖南省湘西州部分规模场牦牛流产衣原体流行情况及风险因素调查

本研究旨在查明湖南省湘西州牦牛流产衣原体感染情况并探讨其可能的风险因素。应用间接血凝试验(IHA)方法检测了该地区的825份牦牛血清,并应用流行病学、统计学方法对牦牛衣原体风险因素进行了评估分析。结果显示,牦牛流产衣原体抗体阳性率为17.2%,季节和性别是牦牛流产衣原体感染的主要风险因素(P0.05)。本研究表明湖南省湘西州牦牛流产衣原体感染率较高,应当对本地区的流产衣原体病采取适当的控制措施,以确保人类和动物的健康安全。     

旋毛虫不同发育时期排泄分泌物抗原在免疫学诊断中的应用研究

[目的]系统了解并比较旋毛虫不同发育时期排泄分泌物(ES)抗原的免疫学特性,探索可用于临床检测出栏猪旋毛虫感染的血清学诊断技术。[方法]分别以旋毛虫肠道期10 h肌幼虫(10 h ML)、肠道期30 h成虫(30 h Ad)、3 d成虫(Ad3)、6 d成虫与新生幼虫混合(Ad6+NBL)以及肌幼虫(ML)五个不同发育时期的ES作为包被抗原,应用ELISA方法,检测感染不同剂量、不同天数的猪血清中的抗旋毛虫抗体Ig M和Ig G水平,绘制抗体消长规律曲线并进行数据分析。[结果]10 h ML ES和ML ES作为包被抗原适合检测不同感染剂量、感染35 d之前的猪抗旋毛虫Ig M抗体,低剂量感染10 d左右可以检出,高剂量感染5 d也可以检出;Ad3 ES作为包被抗原对高剂量感染35 d之前的猪抗旋毛虫Ig M抗体检测敏感;Ad3和ML的ES作为包被抗原可检测不同剂量、感染35 d之后的猪抗旋毛虫Ig G抗体,其中Ad3 ES抗原检测低剂量感染的效果优于ML ES抗原。[结论]肠道期肌幼虫、成虫和肌幼虫的ES抗原可用于检测旋毛虫的早期感染,成虫和肌幼虫的ES抗原可用于检测出栏猪的旋毛虫感染。本研究为进一步合理有效利用旋毛虫不同发育时期的ES抗原,建立更有效的检测屠宰动物旋毛虫感染的方法提供了重要理论基础和参考。     

基于空间分析的森林土壤养分分级方法

为探索一种适用于森林土壤养分分级方法,本研究以广西乐里林场太阳盘分场为研究区域,通过网格化方式布设土壤采样点,分析土壤有效磷含量并进行空间插值,利用森林资源规划数据,提取速生杉木种植小班平均土壤有效磷含量,选取速效氮、速效钾水平中等的小班,计算各小班杉木(Cuninghamiaspp.)年平均生长量,根据相对产量公式计算小班年相对生长量,以"对数"类型拟合年相对生长量与对应土壤养分值之间的关系,分别以相对生长量为50%、75%、90%和95%计算对应的土壤磷素含量,根据计算结果划分土壤磷素丰缺指标,获得该区域土壤有效磷临界值分为2.6、4.0、5.7和6.3,得出研究区杉木林地土壤有效磷分级指标,为制定区域速生林木施肥配方提供研究基础。     

我国森林生态旅游发展刍议

论述了旅游及森林生态旅游的重要性,分析了我国森林生态旅游发展现状、教育现状及研究水平,并指出了我国目前森林生态旅游发展中存在的主要问题及今后的发展方向。