Difference in Carbon Distribution of Initial Photoassimilates between Soybean and Rice as Revealed by 20 s Pulse-300 s Chase Experiments

Short pulse-chase labeling experiments indicated that there was a considerable difference between soybean and rice in the distribution pattern of ¹⁴C to major metabolites during a 300 s chase period. The distribution of ¹⁴C to starch at the end of the chase period was largest in soybean but very small in rice. In rice, the distribution of ¹⁴C to sucrose was the largest. Starch formation during the initial stages of photosynthesis implied the existence of a different mechanism of starch synthesis and utilization between soybean and rice. The relative intensity of the flow into starch increased by treatment with 2% O₂, suggesting that photorespiratory pathway may involve in the regulation of higher starch accumulation in soybean.     

Diagnostic imaging of intra-abdominal cyst in heifer using the computed tomography

A 10-month-old Japanese black heifer was diagnosed as having an intra-abdominal cyst using computed tomography (CT). Through a posterior ventral midline incision, the cyst was removed, and the heifer completely recovered after the surgery. CT scans enabled detection of the intra-abdominal cyst and measurements of the diameter of the cyst before the surgery.     

Lateral resistance of anchor-bolt joints between timber sills and foundations II. Effective lateral resistance of multiple anchor-bolt joints

Monte Carlo simulations were conducted to estimate the effective lateral resistance of multiple anchor-bolt joints with ordinary specifications of Japanese post and beam constructions. Basic lateral load-slip curves of single anchor-bolt joints required in the simulations were determined from the test results of our earlier report. The effective lateral resistance of multiple anchor-bolt joints was estimated for some combinations of loading direction, length/diameter ratio of anchor bolts, lead-hole clearance, and number of anchor bolts. The principal results of the simulations are: (1) anchor-bolt joints loaded perpendicular to lateral forces are not recommended to be counted as supplementary resisting elements because their supplementary shares are far less than those expected from their allowable lateral resistance; (2) multiple anchor-bolt joints with small length/diameter ratios have comparatively lower effective resistance ratios than multiple anchor-bolt joints with large length/diameter ratios; (3) the effective resistance of multiple anchor-bolt joints is affected not only by lead-hole clearance or number of bolts but also variance of load-slip characteristics of single anchor-bolt joints. Part of this work was presented at WCTE2004, Lahti, June 2004     

Analysis of bacterial communities in soil by PCR-DGGE targeting protease genes

We studied the effects of the application of organic (OM) and inorganic fertilizer (CF) on soil protease activity and proteolytic bacterial communities in rhizosphere and bulk soil on an experimental lettuce field in Hokkaido, Japan. The protease activity always was higher in soils of the OM than with the CF treatment, and also higher in the rhizosphere than in the bulk soil. We analyzed proteolytic bacterial communities by denaturing gradient gel electrophoresis (DGGE) of the alkaline metalloprotease (apr) and neutral metalloprotease (npr) genes. Most apr forms detected were closely related to apr of Pseudomonas fluorescens, and all npr variants closely resembled the gene of Bacillus megaterium. These results were consistent with findings from tests using cultured bacterial communities, indicating a high specificity of our PCR-DGGE for amplifying apr and npr genes. The community compositions of proteolytic bacteria were assessed by principal component analysis of the DGGE profiles. There were significant differences in the effects of CF and OM on the community compositions of apr- and npr-expressing bacteria, and the communities of the two types of bacteria played different roles in rhizosphere and bulk soil. We found significant correlations between the protease activity and the communities of the two types of bacteria. The results indicate that different proteolytic bacteria release different amounts or activities of protease, and that the composition of proteolytic bacterial communities may play a major role in determining overall soil protease activity.     

Absence of estrogen receptors delays myoregeneration and leads to intermuscular adipogenesis in a low estrogen status: Morphological comparisons in estrogen receptor alpha and beta knock out mice

This study aimed to investigate the function of estrogen receptors (ERs) in myoregeneration and intermuscular adipogenesis. Ovariectomized (OVX) ERα knockout (KO) mice and ERβ KO mice were used to assess the effect of estrogen on the myoregenerative process. Tibialis anterior muscle was collected on days 7, 10, and 14 after cardiotoxin injection to assess myotube morphology and adipogenesis area. Regenerated myotubes from OVX-ERβ KO mice were consistently smaller in diameter than those from OVX-ERα KO and OVX-wild-type mice, whereas the adipogenesis area of OVX-ERβ KO mice was consistently greater than that of the other types. Therefore, ERβ may be an influential factor in promoting myoregeneration and adipogenesis inhibition compared to ERα.     

DIFFERENCES IN ACQUISITION OF ORGANIC NITROGEN IN SOILS BETWEEN BOK CHOY AND TOMATO

Seedlings of bok choy and tomato were grown in soils with different nitrogen (N) sources [no N (?N), ammonium sulfate (AS), and cattle farmyard manure (CM)]. Comparison between soils treated with ?N and CM indicated that the growth and N accumulation in bok choy were significantly enhanced by CM treatment, whereas no difference was found in tomato. In the rhizosphere soils, the highest protease activity was detected in CM treatment irrespective of species. Correlation analysis between rhizospheric protease activity and total N accumulation of plant treated with –N and CM showed a significant positive correlation only for bok choy. The determination of amino acid absorption rate in excised roots indicated that glycine was taken up at a significantly higher rate in bok choy than tomato. This study suggested that at least two possible factors affected the acquisition of organic N: rhizospheric protease activity and ability to absorb amino acids in roots.     

Shear resistance and failure modes of nailed joints loaded perpendicular to the grain

Shear tests were conducted on nailed joints in wood that were loaded perpendicular to the grain; these joints had 21 specifications depending on different combinations of wood species, nail dimensions, number of nails, and edge distances of the main members, and their effects on the shear resistance of the nailed joints were also investigated. The nailed joints with CN75 nails had higher initial stiffness than the joints with CN50 nails, provided the initial stiffness of nailed joints connected with 3 or 5 nails was not always a simple product of the number of nails and the initial stiffness of nailed joints connected with a nail, and instead depended on the combination of wood species of the main member and nail dimensions. When the edge distance decreased, the maximum load and energy capacity decreased, thereby affecting the energy capacity. The maximum load of the nailed joints with CN75 nails may be smaller than those with CN50 nails depending on the combination of wood species and nail dimensions. When the edge distance of the nailed joints was less than 26 mm, the energy capacity of the nailed joints with CN75 nails was less than or similar to those with CN50 nails.     

Effective lateral resistance of combined timber joints with nails and bolts

An experimental study on combined steel-to-timber joints with nails and bolts is conducted in this study. Principal results are as follows: The initial stiffness and effective allowable resistance of combined joints depend obviously on clearances in predrilled bolt-holes. The combined joints with nails and bolts have high potential of energy capacity to resist strong earthquake forces. There are upper limits of clearances in predrilled bolt-holes that allow advantages of considering the synthetic resistance of combined joints in practical structural design. Combining the joint components with appropriate design will give higher performance against strong earthquakes increasing the safety margin and energy capacity until the failure. The combined joints should be designed under the restrictions of particular specifications in closed design systems because the advantages of combining the joint components are influenced obviously by various actual conditions, which are too difficult to consider in detail in open design systems.     

Evaluation of quality indexes of bending performance and hardness for hardwoods

The mechanical properties of 613 small clear specimens of 35 species (11 ring-porous hardwoods, 19 diffuse-porous hardwoods, and 5 softwoods) were evaluated. The aim of the study was to examine indexes of wood quality that are easy to measure and that exhibit a high correlation with bending performance and hardness that are essential properties of hardwood products. The modulus of rigidity, dynamic modulus of elasticity, bending properties (modulus of elasticity, modulus of rupture, stress at the proportional limit, absorbed energy, Tetmajer’s modulus), dynamic energy absorption by an impact bending test, compressive strength parallel to the grain, shear strength, partial bearing strength, and Brinell’s hardness were measured. A high correlation was found between dynamic modulus of elasticity and static modulus of elasticity. Bending stress at the proportional limit was found to be approximately equivalent to the compressive strength parallel to the grain. Static energy absorption correlated with dynamic energy absorption. Tetmajer’s modulus was found to be closely related to the ratio of the initial stiffness within the elastic range to the secant modulus at the maximum load. A high correlation was observed between Brinell’s hardness and partial bearing strength. The difference in the regression coefficients obtained for these correlations between the species groups was small. Part of this study was presented at the All Division 5 Conference of IUFRO, Taipei, October 2007     

The dynamic state of the ionome in roots,nodules, and shoots of soybean under different nitrogen status and at different growth stages

The relative distribution of 22 mineral elements in the roots, nodules and shoots of the soybean (Glycine max L. Merr. cv. Tsurumusume) at R1 (beginning of the flowering stage) and R7 (beginning of the mature stage) was investigated in response to ammonium and manure N treatment. Plants receiving only atmospheric nitrogen served as the negative control. The addition of ammonium sulfate to the soil caused soil acidification, induced Al and Mn toxicities, and significantly reduced the biomass production in roots and nodules. Ca, Mg, Fe, Mn, Cu, and Zn concentrations were significantly higher in shoots, and those of Mo and Co higher in nodules. The addition of manure to the soil significantly enhanced the levels of Sr, Ba, Cr, and Cd in shoots, whereas the concentration of Cs was decreased at R7. Moreover, when the soybean developed from R1 to R7, the levels of essential elements in nodules decreased, whereas those of nonessential elements increased, irrespective of the nitrogen source. Furthermore, the variation in the concentrations of many elements was not consistent for nodules and roots when soybean developed from R1 to R7. The variation of Mn, Zn, B, and Al concentrations was independent of N treatments. However, Ca, Fe, Cu, Mo, and Se levels were affected strongly by N treatments. This study is the first to document the dynamic variation of the soybean ionome in nodules, roots, and shoots from vegetative to reproductive stage of soybean.