The spawning area of the Japanese eel is located at the southern part of the West Mariana Ridge in the western North Pacific, but their spawning events have not been observed. To further understand Japanese eel spawning ecology, an interdisciplinary research survey by the R/V NATSUSHIMA (NT14-09, 14 May–4 June 2014) was conducted to detect spawning sites based on the seamount, salinity front, new moon and third quadrant (spawning south of front, west of ridge) hypotheses. Attempts were made to film spawning events with underwater camera systems and to consider if eels might be detected in hydroacoustic observations. Although no Japanese eels or spawning events were video-recorded and no eel aggregations could be clearly identified acoustically, three eggs were collected at two stations in the third quadrant region at or just south of 13° N on 26 and 27 May. Three or four days later, newly hatched preleptocephali were collected at two stations far to the south, including 224 at a station > 160 km southwest of the egg catches, and a few preleptocephali were caught at two stations closer to the egg stations. The eggs and southern preleptocephali were from discrete spawning events, which indicated that at least two spawning sites occurred in May 2014.
Although the causal agent of yellows of Brassica rapa (turnip, pak choi, and narinosa) in Japan was reported in 1996 to be Fusarium oxysporum f. sp. conglutinans, this classification has remained inconclusive because of a lack of detailed genetic and pathogenic studies. Therefore, we analyzed the taxonomic position of this organism using Japanese isolates of F. oxysporum complex obtained from diseased individuals of various B. rapa subspecies. Phylogenetic analyses using partial sequences of the rDNA intergenic spacer region and the mating-type gene (MAT1-1-1alpha-box) showed that B. rapa and cabbage isolates belong to different monophyletic clades that separated at early evolutionary stages. Additionally, correlations were observed between the molecular phylogeny and the vegetative compatibility groups. Isolates from turnip, komatsuna, and narinosa (B. rapa group) did not show pathogenicity against cabbage or broccoli (B. oleracea group), although they caused severe symptoms on their original host species. In contrast, cabbage isolates had significantly higher (P = 0.05) virulence on B. oleracea than on B. rapa crops. Our results indicate that F. oxysporum complex isolates from B. rapa and B. oleracea are not only phylogenetically distinct but also differ in host specificity. Therefore, we propose a novel forma specialis, F. oxysporum f. sp. rapae, which causes yellows on B. rapa, including turnip, komatsuna, pak choi, and narinosa. 相似文献
A sophisticated modeling approach for simulating-coupled surface and subsurface flows in a watershed is presented. The watershed
model developed is a spatially distributed physically based model of composite dimension, consisting of 3-D variably saturated
groundwater flow submodel, 2-D overland flow submodel and 1-D river flow submodel. The 3-D subsurface flow is represented
by the complete Richards equation, while the 2-D and 1-D surface flows by the diffusive approximations of their complete dynamic
equations. For piecewise integration of these equations, the finite volume method (FVM) is employed assuming unknown variables
such as the water depth and the pressure head to be volume-averaged state ones. Problem plane geometry is meshed with the
unstructured cells of triangular shape which conforms to external as well as internal irregular boundaries such as those between
1-D and 2-D flows. A cell size controlling scheme, referred to as quasi-adaptive meshing scheme, is introduced to keep the
local discretization errors caused by topographic elevation gradient even over the entire-meshed geometry. Performance of
the model is tested through its practical application to a rugged intermountain watershed. Tuning the values of the three
key parameters ensures successful calibration of the model. Once the model is so calibrated, it could reproduce satisfactory
runoff response to any rainfall event. Expansion and shrinkage of the contributing area importantly affecting the direct runoff,
caused by the vicissitude of rainfall during its total duration, are well reproduced, like what the commonly accepted runoff
theory argues. It is thus concluded that the model developed could serve as a powerful watershed simulator usable for investigating
and assessing the hydrological aspect of a watershed. 相似文献
Eastern little tuna (ELT, Euthynnus affinis) is expected to see use as a novel aquaculture species due to its rapid growth and high economic value. In this study, closing of the complete life cycle of ELT in land‐based tanks was successfully carried out. Seed production of a first generation (F1) derived from wild‐caught ELT broodstock was conducted in 2010, 2011 and 2012. Survival percentages of F1 ELT at 365 days post hatching for the 2010, 2011 and 2012 year classes were 0.9%, 0.4% and 6.3% respectively. On average, F1 ELT were approximately 40 cm in total length and weighed 1500 g by the time they reached 1 year of age. Successful artificial induction of spawning was achieved in 1‐year‐old F1 ELT using a gonadotropin‐releasing hormone analogue (GnRHa). Induction of spawning was successful at the first administration of GnRHa in the 2010, 2011 and 2012 year classes. First spawning was observed at 4 or 5 days post implantation, which indicated that oogenesis and spermatogenesis progressed within 1 year of rearing in land‐based tanks. During spawning periods, the average number of fertilized eggs and the average number of hatched larvae per spawning event in the 2010, 2011 and 2012 year classes were 20,480 eggs day?1 and 177 larvae day?1, 39,423 eggs day?1 and 9347 larvae day?1, and 819,555 eggs day?1 and 674,445 larvae day?1 respectively. Therefore, it was concluded that both male and female ELT reared in land‐based tanks were capable of reproduction at 1 year of age. 相似文献
The purpose of this study was to evaluate the effects of polyphenols from seed shells of Japanese horse chestnut (JHP) on methotrexate (MTX)-induced intestinal injury in rats. MTX application caused intestinal morphological injury and increase in malondialdehyde (MDA) levels, decrease in levels of glutathione (GSH) and glutathione peroxidase (GSH-Px) activities in small intestine. However, oral administration of JHP ameliorated MTX-induced intestinal injury and inhibited the increase in MDA and the decrease in GSH and GSH-Px activity in small intestine. In conclusion, our results indicated that oral administration of JHP alleviated MTX-induced intestinal injury through its antioxidant properties. 相似文献
The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event. 相似文献
A fuzzy optimization model is developed to allocate allowable total nitrogen (T-N) loads to distributed nonpoint sources (NPSs)
and point sources (PSs) in a watershed for river water quality management using the linear programing technique. The watershed
is divided into uniform grid cells on which T-N loads issuing from NPSs such as paddy fields, upland crop fields and cities
are controlled. A geographic information system integrated with the digital elevation model facilitates computation of route
lengths of surface and subsurface flows from cells to a river running through the watershed. The T-N loads discharged from
their sources are assumed to decay, subject to distance-related first-order kinetics. As management goals, maximizations of
total allowable NPS loads, total allowable PS loads and total yield of rice are considered from environmental and economic
viewpoints. A prime constraint is an effluent limitation standard for the aggregate amount of loads that arrive at the downstream
end of the river. The fuzzy sets theory helps appropriately describe vague attitudes of decision-makers (i.e., stakeholders
and management authorities) in terms of constraints and conflicting goals. An application of the fuzzy optimization model,
developed as an improvement over our last nonfuzzy model, to a real watershed in Shiga prefecture, Japan, demonstrates that
the fuzzy model embodies our last model, and is capable of creating management alternatives for T-N load allocation in a more
practical and flexible manner. 相似文献
A cell-based distributed watershed model is developed which enables us to simulate the hydrological and hydraulic aspects
of the watershed in a refined fashion. With three-zoned cell profiling, the model is composed of three sub-models; tank model
for a surface water zone, soil moisture model for a surface soil zone, and unconfined shallow groundwater flow model for a
subsurface zone. Inclusion of the soil moisture sub-model modified to reroute the infiltration, routed from the tank sub-model,
into the return flow and the groundwater recharge features the model. The groundwater flow sub-model, numerically approximated
by use of the finite volume method and the implicit time-marching scheme, considers a network of on-farm drainage canals as
internal boundaries, which is an essential need for modeling the watershed including farmlands. Cascade-linking of the three
sub-models in a cell and assembling of all the cells over the entire watershed domain provides the global equations system
to be solved. Applicability of the model is demonstrated with its practical application to a real watershed in that paddy
and upland crop fields take great part of the land-use practice. It is then indicated in a quantified manner that rice farming
significantly contribute as a major groundwater recharger in an irrigation period to fostering and conservation of regional
water resources. Along with appropriately profiling a cell, the model is so versatile and tough that it can be applied without
difficulty to a watershed of diverse terrains and land-uses and the computations can stably be carried out. It is thus concluded
that the model presently developed could be a powerful “watershed simulator” to investigate and assess the time-varying hydro-environmental
properties of a watershed while separating and integrating the hydrological and hydraulic components of particular importance. 相似文献
To further our understanding of wood decay in living light red meranti (Shorea smithiana) trees, microscopic characteristics of the cell and cell wall degradations of S. smithiana wood in the presence of the decay fungi, the identity of the causal fungi, and the decay potential and pattern by an isolated
fungus were investigated. Cell wall degradations, including cell wall thinning, bore holes formation, rounded pit erosion,
and eroded channel opening were clearly observed under light and scanning electron microscopy. In transverse view, many large
voids resulting from a coalition of degraded wood tissue appeared in the decayed canker zone. All these observations suggest
the well-known simultaneous decay pattern caused by white-rot fungi. By phylogenetic analysis based on the sequences of internal
transcribed spacer region of ribosomal DNA, a basidiomycete fungus isolated from the decayed wood was identified as Schizophyllum commune. The degradation caused by this fungus on sound S. smithiana wood in an in situ laboratory decay test was classified as the early stage of simultaneous decay, and showed a similar pattern
to that observed in the wood samples naturally decayed. 相似文献