Ascorbic acid triggered physiochemical transformations at different phenological stages of heat‐stressed Bt cotton

Heat stress at reproductive stages of cotton crop is the main cause for instability in yield than other abiotic stresses of crops. An experiment was conducted to observe the impacts of heat stress at squaring and flowering stages of cotton and potential of exogenously applied ascorbic acid to alleviate this stress. The experiment was planted for two consecutive years, that is 2013 and 2014, in randomized complete block design with split plot arrangement having three replications. Treatments were comprised of heat stress in main plots, viz. H₀ = no heat imposition; H₁ = heat imposition at square initiation; H₂ = heat imposition at flower initiation and foliar‐applied ascorbic acid in subplots, viz. A₀ = control or water spray; A₁ = 20 mg/L; A₂ = 40 mg/L; and A₃ = 60 mg/L. Results showed that heat stress badly affected plant water relations (relative water contents and osmotic potential), total chlorophyll contents and activities of antioxidant enzymes that led to a corresponding decrease in seed cotton yield. Foliar application of ascorbic acid (40–60 mg/L) ameliorated adverse effects of heat and thus helped the crop to improve its water relations, stay green and antioxidant enzymes resulting into better crop yield.     

Molecular cloning, expression and characterization of a functional GSTmu class from the cattle tick Boophilus annulatus

A full-length cDNA of a glutathione S-transferase (GST) was cloned from a cDNA library of the local Egyptian cattle tick Boophilus annulatus. The 672 bp cloned fragment was sequenced and showed an open reading frame encoding a protein of 223 amino acids. Comparison of the deduced amino acid sequence with GSTs from other species revealed that the sequence is closely related to the mammalian mu-class GST. The cloned gene was expressed in E. coli under T7 promotor of pET-30b vector, and purified under native conditions. The purified enzyme appeared as a single band on 12% SDS-PAGE and has a molecular weight of 30.8 kDa including the histidine tag of the vector. The purified enzyme was assayed upon the chromogenic substrate 1-chloro-2,4-dinitrobenzene (CDNB) and the recombinant enzyme showed high level of activity even in the presence of the beta-galactosidase region on its 5' end and showed maximum activity at pH 7.5. The Km values for CDNB and GSH were 0.57 and 0.79 mM, respectively. The over expressed rBaGST showed high activity toward CDNB (121 units/mg protein) and less toward DCNB (29.3 units/mg protein). rBaGST exhibited peroxidatic activity on cumene hydroperoxide sharing this property with GSTs belonging to the GST alpha class. I50 values for cibacron blue and bromosulfophthalein were 0.22 and 8.45 microM, respectively, sharing this property with the mammalian GSTmu class. Immunoblotting revealed the presence of the GST molecule in B. annulatus protein extracts; whole tick, larvae, gut, salivary gland and ovary. Homologues to the GSTmu were also detected in other tick species as Hyalomma dromedarii and Rhipicephalus sp. while in Ornithodoros moubata, GSTmu homologue could not be detected.     

Governance of forest conservation and co-benefits for Bangladesh under changing climate

We focused on key aspects of forest governance for biodiver- sity conservation in implementing new climate change policies. The national forest institutions must be adaptive to identify the existing pitfalls of prior conservation policies to take advantage of new climate change policies. Strengthening roles and technical capacity of national institutions for systematic biodiversity monitoring and carbon stock assessment is required in developing and least developed countries. Community participation needs careful analysis to ensure equitable ac- cess of particular social groups to local decision-making processes and to sustain optional livelihoods. The livelihood options around forest reserves or protected areas must be taken into account to enhance forest-based adaptation.     

Normal anatomic reference of pastern and coffin joints in Egyptian buffalo (Bubalus bubalis): A compared atlas of cross-sectional anatomy,magnetic resonance imaging and computed tomography

The purpose of this study was to describe normal magnetic resonance imaging and computed tomographic anatomy of pastern and coffin joints in Egyptian buffalo using cadaveric distal limbs. This study was achieved using twelve fresh cadaveric distal limbs from adult healthy buffaloes of both sexes. These cadaveric limbs were scanned using a 1 Tesla MRI scanner and CT scanner, injected with red latex, frozen at −20°C for 1 week, and then sectioned into sagittal, dorsal and transverse slices. The obtained MR and CT images were selected to be matched with their corresponding anatomical cross-sections for identification and evaluation of the clinically correlated anatomical structures of the pastern and coffin joints. The difference in signal intensities on CT and MRI scans amongst the tissues allowed clear differentiation of major bone and soft tissue structures of the pastern and coffin joints. CT provided a high spatial resolution of bone and soft tissue structures, however, MRI allowed a better and higher resolution and definition between soft tissues. The current study provided a normal CT and MRI anatomic reference which could help veterinary clinicians for interpretation and diagnosis of the clinically affected pastern and coffin joints in buffalo.     

Production and economic return of shrimp aquaculture in coastal ponds of different sizes and with different management regimes

Coastal shrimp (Penaeus monodon) aquaculture in Bangladesh is mostly practiced in a special type of field/pond situated by the side of a river -- called a Gher -- that is used to cultivate rice in winter and shrimp in summer. Ghers of different sizes are managed in different ways. In order to understand the effects of Gher size and their corresponding management practices on the production and economic returns of shrimp farming, we conducted an on-farm study in three small (1–5 ha), three medium (6–10 ha) and three large (>10 ha) Ghers located in the coastal Southwest region of Bangladesh. The mean harvest weight of shrimp was similar in all Ghers, but survival rates were higher in the small (50%) and medium (37%) ones than in the large (18%) ones. The high mortality in the large units led to a production that was lower than one-half of that in the small Ghers (about 80 and 200 kg/ha, respectively). The total variable costs per unit area were similar in all Ghers, but the importance of different items varied with Gher size. In the small Ghers, there are relatively high investments on inputs and labor. This expenditure results in lower shrimp mortality and higher production, which in turn results in gross returns that are larger than the total costs -- hence, a positive and high net return. In large Ghers, the fixed costs are larger than in the small ones simply because of the size of the pond. Variable costs include higher investments in post-larvae and labor than in small Ghers, and lower inputs applied only at pond preparation. This results in higher shrimp mortality and lower production, which in turn results in gross returns that are smaller than the total costs -- hence, a negative or low net return. These findings have positive implications for the future policy formulation of coastal shrimp aquaculture in Bangladesh.     

Strategies for maximizing utilization of de-oiled rice bran (DORB) in the fish feed

De-oiled rice bran (DORB) is obtained after oil is removed from the rice bran. This is the commonly used ingredient in the feeding of Indian major carps due to its cheaper cost. DORB is used as a feed ingredient in cattle, poultry, and fish diet. Along with this, they are also used in making fiber-rich biscuits and extraction of an antioxidant compound like oryzanol and other industrial uses. Due to the increased competition, there will be a huge imbalance in the demand and supply for this ingredient in the years to come. DORB is associated with high fiber content, anti-nutrients, and non-starch polysaccharides which lead to its poor utilization resulting in a higher feed conversion ratio (FCR) in fish. Hence, the strategic utilization of this ingredient by solid-state fermentation, exogenous enzyme supplementation, and supplementing it with deficient nutrients like amino acids and fatty acids will help in improving the utilization of this ingredient.

     

Biotic and abiotic drivers of intraspecific trait variation within plant populations of three herbaceous plant species along a latitudinal gradient

Background

The importance of intraspecific trait variation (ITV) is increasingly acknowledged among plant ecologists. However, our understanding of what drives ITV between individual plants (ITV_BI) at the population level is still limited. Contrasting theoretical hypotheses state that ITV_BI can be either suppressed (stress-reduced plasticity hypothesis) or enhanced (stress-induced variability hypothesis) under high abiotic stress. Similarly, other hypotheses predict either suppressed (niche packing hypothesis) or enhanced ITV_BI (individual variation hypothesis) under high niche packing in species rich communities. In this study we assess the relative effects of both abiotic and biotic niche effects on ITV_BI of four functional traits (leaf area, specific leaf area, plant height and seed mass), for three herbaceous plant species across a 2300 km long gradient in Europe. The study species were the slow colonizing Anemone nemorosa, a species with intermediate colonization rates, Milium effusum, and the fast colonizing, non-native Impatiens glandulifera.

Results

Climatic stress consistently increased ITV_BI across species and traits. Soil nutrient stress, on the other hand, reduced ITV_BI for A. nemorosa and I. glandulifera, but had a reversed effect for M. effusum. We furthermore observed a reversed effect of high niche packing on ITV_BI for the fast colonizing non-native I. glandulifera (increased ITV_BI), as compared to the slow colonizing native A. nemorosa and M. effusum (reduced ITV_BI). Additionally, ITV_BI in the fast colonizing species tended to be highest for the vegetative traits plant height and leaf area, but lowest for the measured generative trait seed mass.

Conclusions

This study shows that stress can both reduce and increase ITV_BI, seemingly supporting both the stress-reduced plasticity and stress-induced variability hypotheses. Similarly, niche packing effects on ITV_BI supported both the niche packing hypothesis and the individual variation hypothesis. These results clearly illustrates the importance of simultaneously evaluating both abiotic and biotic factors on ITV_BI. This study adds to the growing realization that within-population trait variation should not be ignored and can provide valuable ecological insights.

     

Generation of interspecific hybrids between <Emphasis Type="Italic">Trifolium vesiculosum</Emphasis> and <Emphasis Type="Italic">T. alexandrinum</Emphasis> using embryo rescue

Interspecific hybrids were developed between Trifolium alexandrinum cultivar Wardan × Trifolium vesiculosum and T. alexandrinum cultivar BL1 × T. vesiculosum through embryo rescue, as the crosses failed to set seed under natural conditions. Trifolium vesiculosum was used as a donor/male parent in this study as it is reported to possess tolerance to stem rot and high forage yield. Fertilization in crossed florets of the crosses was manifested from the recovery of swollen ovaries (< 7.80%) and confirmed from the presence of one degenerated ovule in most (> 93.00%) of the swollen ovaries. The hybrid embryos at various developmental stages (heart, torpedo and cotyledonary) were rescued at a frequency of 2.56% from Wardan × T. vesiculosum and 6.12% from BL1 × T. vesiculosum. Differentiation occurred only in the cotyledonary stage embryos, resulting in 17 putative interspecific hybrid plantlets. The assessment of plantlet hybridity through SSR markers (for the alleles inherited from the donor parent), micromorphological leaf traits (leaf texture and stomata) and morphological characters (plant height, leaflet length and width) confirmed production of two interspecific hybrids designated as AV1 and BV3 representing both the crosses. AV1 displayed moderate resistance and BV3 was resistant to stem rot.     

Limited survival of Ralstonia solanacearum Race 3 in bulk soils and composts from Egypt

Survival of Ralstonia solanacearum race 3 biovar 2 (phylotype II sequevar 1) in Egyptian soils and compost was studied under laboratory and field conditions. Survival of the pathogen under laboratory conditions varied with temperature, water potential and soil type, with temperature being the major determinant of survival of the pathogen. The effects of temperature and moisture content were variable between different experiments, but survival was generally longer at 15°C than at 4, 28 and 35°C respectively. Survival was also longer when moisture levels were constant compared with varying moisture levels at all temperatures. In experiments to compare the effects of progressive drying in sandy and clay soils there was a difference in survival times between the two soil types. In sandy soils, the pathogen died out more rapidly when soil was allowed to dry out than in controls where the soil was kept at constant water potential. In clay soils there was little difference between the two treatments, possibly due to the formation of a hard impermeable outer layer during the drying process, which retarded water loss from within. Survival in mature composts at 15°C was of the same order of magnitude as in soils but shorter at 28°C, possibly owing to increased biological activity at this temperature, or a resumption of the composting process, with concomitant higher temperatures within the compost itself. The maximum survival time recorded over all soil types and conditions during in vitro studies was around 200 days. In field studies, the maximum survival time in both bare sand and clay was around 85 days at depths up to 50 cm. The survival time was reduced in field experiments carried out in summer to less than 40 days and in one study when the ground was flooded for rice cultivation, the bacterium could not be detected 14 days after flooding. The maximum survival time of R. solanacearum in infected plant material or in infested soil samples incorporated into compost heaps was less than 2 weeks. At the culmination of field soil and compost experiments, no infection was detected in tomato seedlings up to 10 weeks after transplanting into the same soils or composts under glasshouse conditions at a temperature of 25°C.