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排序方式: 共有423条查询结果,搜索用时 31 毫秒
61.
Joohyun Jung Jinhwa Chang Sunkyoung Oh Mincheol Choi 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(2):155-159
Spectral waveform analysis of blood flow velocity in the major arteries of six healthy, conscious immature micropigs was determined using Doppler ultrasonography. Doppler spectral tracings were recorded from the external iliac artery, femoral artery, and renal arcuate artery. Tracings were also taken from three parts of the common carotid artery and two parts of the abdominal aorta. Spectral Doppler parameters included peak systolic velocity, early diastolic velocity, peak systolic velocity-to-end diastolic velocity ratio, resistive index, and pulsatility index. In addition, the diameter of major arteries and indirect blood pressure were measured. These results from spectral Doppler analysis in major arteries may be useful as reference ranges in the future studies of vascular hemodynamics in immature micropigs. 相似文献
62.
Young Hoon Park Seong Gyu Ahn Young Mi Choi Hye Jeong Oh Dong Chun Ahn Jin Gi Kim Jum Soon Kang Young Whan Choi Byoung Ryong Jeong 《Scientia Horticulturae》2010
The ‘Genome database for Rosaceae (GDR)’ provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei–Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King’ and ‘Carnival’) to 0.99 (‘First Red’ and ‘Red Champ’). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang’ and ‘Janghyee’) to 0.64 (‘Janghyee’ and ‘Pragana’). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana’ was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae. 相似文献
63.
Kim Hong-Sik Kang Beom-Kyu Seo Jeong-Hyun Kim Hyun-Tae Ha Tae-Joung Oh Jae-Hyeon Shin Sang-Ouk Baek In-Yeol 《Journal of Crop Science and Biotechnology》2021,24(2):179-190
Journal of Crop Science and Biotechnology - There is great interest in the enhancement of isoflavones as one of the functional ingredients in soybean. This study aimed to investigate the effects of... 相似文献
64.
Park JM Kim M Min J Lee SM Shin KS Oh SD Oh SJ Kim YH 《Journal of agricultural and food chemistry》2012,60(16):4053-4059
For the identification of a novel insecticidal protein, a two-dimensional liquid chromatography (PF-2D) system was used in a quantitative proteomic analysis of Xenorhabdus nematophila CBNU strain isolated from entomophagous nematode Steinernema carpocapsae . Protein patterns obtained from minimum and maximum insecticidal activities during cultivation were contrasted, and a novel toxin protein (Txp40) was identified by MALDI-TOF/MS. The DNA sequence of the cloned toxin gene (1089 bp) has an open reading frame encoding 363 amino acids with a predicted molecular mass of 41162 Da. The txp40 identified in this study is most closely related to the known txp40 cloned from X. nematophila EB (ADQ92844) with 94.4% identical sequence residues. Following the expression of the newly identified toxin gene in Escherichia coli , the insecticidal activity of the recombinant toxin protein was determined against Plutella xylostella larvae; a 56.7% mortality rate was observed within 24 h. 相似文献
65.
Sang?Youn?Oh Dong?Il?YooEmail author Younsook?Shin Wha?Seop?Lee Seong?Mu?Jo 《Fibers and Polymers》2002,3(1):1-7
Cellulose carbonate was prepared by the reaction of cellulose pulp and CO2 with treatment reagents, such as aqueous ZnCl2 (20–40 wt%) solution, acetone or ethyl acetate, at −5–0°C and 30–40 bar (CO2) for 2 hr. Among the treatment reagents, ethyl acetate was the most effective. Cellulose carbonate was dissolved in 10% sodium
hydroxide solution containing zinc oxide up to 3 wt% at −5–0°C. Intrinsic viscosities of raw cellulose and cellulose carbonate
were measured with an Ubbelohde viscometer using 0.5 M cupriethylenediamine hydroxide (cuen) as a solvent at 20°C according
to ASTM D1795 method. The molecular weight of cellulose was rarely changed by carbonation. Solubility of cellulose carbonate
was tested by optical microscopic observation, UV absorbance and viscosity measurement. Phase diagram of cellulose carbonate
was obtained by combining the results of solubility evaluation. Maximum concentration of cellulose carbonate for soluble zone
was increased with increasing zinc oxide content. Cellulose carbonate solution in good soluble zone was transparent and showed
the lowest absorbance and the highest viscosity. The cellulose carbonate and its solution were stable in refrigerator (−5°C
and atmospheric pressure). 相似文献
66.
Young Min Im Tae Hwan Oh Jin Wook Cha Young Ho Seo Jun Sung Hwang Joseph A. Nathanael Sung Soo Han Soon Ho Jang 《Fibers and Polymers》2014,15(10):2066-2071
Poly(vinyl alcohol) (PVA)/zirconium oxide (ZrO2) composite nanofibers with a skin-core structure were prepared and the effect of ZrO2 particle content on uniform web formation was investigated. The optimized polymer concentration, tip to collector distance, and applied voltage for electrospinning were 11 wt%, 12 cm, and 20 kV, respectively. Skin-core PVA/ZrO2 composite nanofibers containing up to 12 wt% ZrO2 were successfully prepared, but it was difficult to obtain PVA/ZrO2 composite nanofiber webs via conventional electrospinning. Increasing the amount of ZrO2 caused the morphology of the PVA/ZrO2 composite nanofibers to become a non-uniform nanoweb with irregular nanofiber diameters. While it was difficult to obtain a uniform nanofiber web containing a content of ZrO2 over 6 wt% for conventional electrospinning, a more uniform nanofiber web could be obtained at up to 9 wt% ZrO2 using a skin-core dual nozzle. More uniform webs could also be obtained when ZrO2 was in the skin rather than the core. 相似文献
67.
Ionic conductivity and mechanical properties of a mixed polymer matrix consisting of poly(ethylene glycol) (PEG) and cyanoresin
type M (CRM) with various lithium salts and plasticizer were examined. The CRM used was a copolymer of cyanoethyl pullulan
and cyanoethyl poly(vinyl alcohol) with a molar ratio of 1:1, mixed plasticizer was ethylene carbonate (EC) and propylene
carbonate (PC) at a volume ratio of 1:1. The conductive behavior of polymer electrolytes in the temperature range of 298∼338
K was investigated. The PEG/LiClO4 complexes exhibited the highest ionic conductivity of ∼10−5 S/cm at 25°C with the salt concentration of 1.5 M. In addition, the plasticized PEG/LiClO4 complexes exhibited improvement of ionic conductivity. However, their complexes showed decreased mechanical properties. The
improvement of ionic conductivity and mechanical properties could be obtained from the polymer electrolytes by using CRM.
The highest ionic conductivity of PEG/CRM/LiClO4/(EC-PC) was 5.33×10−4 S/cm at 25°C. 相似文献
68.
Polylactide(PLA) films were drawn at various drawing temperature of 65, 90 and 120 °C. The effects of drawing temperature on structural conformation and properties of PLA films were investigated. It was confirmed that the PLA films at drawing temperature of 65 and 90 °C were composed of α′ phase crystal form. The strain-induced crystallization and molecular orientation increased with increasing the draw ratio, which result in improving the mechanical and thermal properties of α′ phase PLA films. However, at drawing temperature of 120 °C, the strain-induced crystallization and molecular orientation of PLA films were not distinctly detected. It was supposed that the rate of the chain relaxation was faster than chain orientation and strain-induced crystallization during uniaxial drawing process. 相似文献
69.
Yang DK Shin EK Oh YI Kang HK Lee KW Cho SD Song JY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1077-1082
We analyzed the nucleotide sequences of the G-L (glycoprotein-large protein) intergenic non-coding region of 33 strains of the rabies virus (RABV) isolated in South Korea in 1998-2010 and compared the sequences with those of previously reported non-Korean strains. The similarities of the nucleotide sequences of the G-L region among all Korean RABV isolates ranged from 97.1 to 100%. Based on the phylogenetic analysis of the G-L region, the Korean RABV isolates were classified into three distinct subgroups with high similarity and were most closely related to the non-Korean NeiMeng1025C isolate, which was isolated from a rabid raccoon dog in eastern China, suggesting that the Korean RABV isolates originate from a rabid raccoon dog in northeastern Asia. Our results indicated that G-L region, as a useful phylogenetic indicator, is equivalent to the nucleoprotein (N) or glycoprotein (G) gene for study of RABV molecular epidemiology and that the Korean RABV isolates showing a few substitutions in the G-L region are continuously circulating in South Korea. 相似文献
70.
Kim B Shin JH Han K Seo HW Oh Y Kang I Park C Lee BH Jang JS Kim SH Chae C 《Veterinary research communications》2011,35(7):469-475
The expression of myeloperoxidase (MPO) was examined in the swine influenza virus (SIV)-infected neutrophils in the lungs of pigs experimentally infected with swine influenza virus (SIV) subtype H1N2 by immunohistochemistry. Five pigs each from the infected and non-infected group were euthanized 1, 3, 5, 7, and 10?days post-inoculation (dpi). Immunohistochemical reactivity was mainly seen in neutrophils. The score for pulmonary histopathological lesions correlated with the score for MPO immunohistochemical reactivity (r ( s )?=?0.962, P?0.01). In addition, the score for in situ hybridization of SIV nucleic acid correlated with the score for MPO immunohistochemical reactivity (r ( s )?=?0.976, P?0.01). These results suggest neutrophils are one of the primary effector cells in the early phase of SIV infection in pigs. 相似文献