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411.
412.
Many apoptotic molecules relocate subcellularly in cells undergoing apoptosis. The pro-apoptotic protein BID underwent posttranslational (rather than classic cotranslational) N-myristoylation when cleavage by caspase 8 caused exposure of a glycine residue. N-myristoylation enabled the targeting of a complex of p7 and myristoylated p15 fragments of BID to artificial membranes bearing the lipid composition of mitochondria, as well as to intact mitochondria. This post-proteolytic N-myristoylation serves as an activating switch, enhancing BID-induced release of cytochrome c and cell death. 相似文献
413.
Kwan Woo Kim Keun Hyung Lee Bong Seok Lee Yo Seung Ho Seung Jin Oh Hak Yong Kim 《Fibers and Polymers》2005,6(2):121-126
Semicrystalline poly(ethylene terephthalate) (cPET)/amorphous poly(ethylene terephthalate) with isophthalic acid (aPET) blends
with 100/0, 75/25, 50/50, 25/75, and 0/100 by weight ratios were dissolved in a mixture of trifluoroacetic acid (TFA)/methylene
chloride (MC) (50/50, v/v) and electrospun via the electrospinning technique. Solution properties such as solution viscosity,
surface tension and electric conductivity were determined. The solution viscosity slightly decreased as aPET content increased,
while there was no difference in surface tension with respect to aPET composition. The characteristics of the electrospun
cPET/aPET blend nonwovens were investigated in terms of their morphology, pore size and gas permeability. All these measurements
were carried out before and after heat treatment for various blend weight ratios. The average diameter of the fibers decreased
with increasing aPET composition due to the decrease in viscosity. Also, the morphology of the electrospun cPET/aPET blend
nonwovens was changed by heat treatment. The pore size and pore size distribution varied greatly from a few nanometers to
a few microns. The gas permeability after heat treatment was lower than that before heat treatment because of the change of
the morphology. 相似文献
414.
The ability to absorb liquid and the dust removal performance are important factors for wiping cloths used to remove contaminants. We have developed a method that can determine the contaminant removal performance of wiping cloths. In the gravimetric method, experimental errors are unavoidable because the contaminant plate is much heavier than the contaminant material. However, we used image analysis to reduce the experimental errors, and did not use the heavy contaminant plate. The correlation coefficient between the image analysis and the gravimetric methods was very high, at R=0.97, with a significance level of 95 %. From the correlation analysis and empirical data, the image analysis method is a useful tool for measuring wiping efficiency. The wiping efficiency measured using image analysis has a close relationship to the wiping speed, viscosity of the contaminant, and wiping pressure, at the significance level of 95 %. 相似文献
415.
K. Park V.R. Dirisala Y. Oh H. Choi K.T. Lee J.H. Kim H.T. Lee K.H. Seo & C. Park 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(2):127-133
Sequences from the clones of full-length enriched cDNA libraries serve as valuable resources for functional genomic studies. We have analysed 1970 high-quality chromatograms (Phred value ≥ 30) that were obtained from sequencing the 5' ends of brainstem, liver, neocortex and spleen clones derived from full-length enriched cDNA libraries from Korean native pigs. In addition, 50 000 pig expressed sequence tag (EST) sequence trace files were obtained from Genbank and combined with our sequencing information to facilitate SNP identification in silico . The process generated 8118 contigs, of which 239 included minimum one sequence from Korean native pig and contained 678 putative coding single nucleotide polymorphisms (cSNPs). Of these, 33 putative cSNPs were randomly selected for confirmatory analysis and validated using 20 pigs from four different breeds (Duroc, Landrace, Yorkshire, Korean native pig). Of the 33 putative cSNPs, 20 were confirmed (61%), which was similar to the frequency reported in other studies. We also identified 15 new cSNPs from the validation process, which were not detected by our in silico analysis. Our study shows that analysing genetically diverse pig breeds including the Korean native pig could serve as a useful strategy for generating a large number of cSNPs. 相似文献
416.
Estimation of instantaneous and daily net radiation from MODIS data under clear sky conditions: a case study in East Asia 总被引:2,自引:0,他引:2
The Moderate Resolution Imaging Spectroradiometer-based net radiation (R N) model was built and applied in East Asia in 2005. Because there have hardly been simple parameterization schemes developed over a large area using remote-sensing technology, the model was aimed to present physical simplicity in complex topography at multiple spatiotemporal scales. The model successfully reproduced the instantaneous R N values obtained at four flux tower sites having individually different ecohydrology. The diurnal cycle of R N was contextually simulated using a simple sine curve to determine the daily and monthly average net radiation. The diurnal R N estimation method was proven to be a reliable model as long as accurate boundary conditions, sunrise and sunset times, for example, were obtained. The monthly average net radiation (MANR) was estimated using the diurnal patterns of the instantaneous R N. Distribution of the monthly R N demonstrated that elevation and latitude were the primary factors affecting the MANR. The proposed R N algorithm turned out to be a promising method for valuable applications in various fields due to systematic simplicity and fewer input parameters. 相似文献
417.
Inhibition of the pathogenicity of Magnaporthe grisea by bromophenols, isocitrate lyase inhibitors, from the red alga Odonthalia corymbifera 总被引:1,自引:0,他引:1
Lee HS Lee TH Lee JH Chae CS Chung SC Shin DS Shin J Oh KB 《Journal of agricultural and food chemistry》2007,55(17):6923-6928
Magnaporthe grisea is a fungal pathogen of rice that forms appressoria that penetrate the outer cuticle of the rice plant. Data from recent studies indicate that M. grisea isocitrate lyase (ICL), a key enzyme in the glyoxylate cycle, is highly expressed during appressorium-mediated plant infection. Bromophenols isolated from the red alga Odonthalia corymbifera exhibited potent ICL inhibitory activity and blocked appressoria formation by M. grisea in a concentration-dependent manner. In addition, these compounds protected the rice plants from infection by M. grisea. Rice plants infected with wild-type M. grisea Guy 11 exhibited significantly lower disease severity with bromophenol treatment than without, and the treatment effect was comparable to the behavior of the Deltaicl knockout mutant I-10. The protective effect of bromophenols and their strong inhibition of appressorium formation on rice plants suggest that ICL inhibitors may be promising candidates for crop protection, particularly to protect rice plants against M. grisea. 相似文献
418.
J-Y Song K Sasaki T Okada M Sakashita H Kawakami S Matsuoka H-S Kang K Nakayama S-J Jung M-J Oh S-I Kitamura 《Journal of fish diseases》2009,32(12):1027-1034
In Japan and Korea, outbreaks of scuticociliatosis have frequently occurred in Japanese flounder, Paralichthys olivaceus . Morphological observations and small subunit rRNA gene sequences have shown that the causative agent of scuticociliatosis in the flounder is Miamiensis avidus (syn. Philasterides dicentrarchi ). In this study, we elucidated the antigenic differences between six Japanese M. avidus isolates as an initial step toward developing an effective vaccine against the disease. Four Japanese flounder isolates (IyoI, Nakajima, JF05To and Mie0301 isolates), one spotted knifejaw, Oplegnathus punctatus, isolate (SK05Kyo), and one ridged-eye flounder, Pleuronichthys cornutus , isolate (RF05To) were subjected to serological analysis. Antisera against IyoI, SK05Kyo, Nakajima and Mie0301 isolates were raised in rabbits and used for immobilization assays and Western blotting. Immobilization assays showed that the six isolates could be divided into three groups, tentatively designated serotype I for IyoI, JF05To, RF05To, SK05Kyo, serotype II for Nakajima and serotype III for Mie0301. Western blotting results supported these three serotypes, with marked similarities in the banding profiles of IyoI, JF05To, RF05To and SK05Kyo isolates, which were distinct from the Nakajima and Mie0301 isolates. Three isolates, IyoI, Nakajima and Mie0301 that were selected as representatives of each serotype, were highly pathogenic to Japanese flounder by experimental infection. Based on these findings, we propose that there are at least three M. avidus serotypes in Japan. 相似文献
419.
Studies were conducted by injecting/feeding white spot syndrome virus (WSSV) derived from infected shrimp, Penaeus monodon (Fabricius), to different life-stages, namely post-larvae, juveniles, sub-adults and adults of Macrobrachium rosenbergii (de Man). The disease was also induced in brood stock, and the eggs and larvae derived from these animals were subsequently tested for WSSV infection. All the stages except egg used for the experiment were found WSSV positive in histopathology, cross infection bioassay and polymerase chain reaction (PCR) analysis. Experimentally infected post-larvae and juveniles showed a high percentage of mortality and an increased rate of cannibalism. The cumulative mortality in post-larvae was up to 28%; with 28–40% cannibalism resulting in a maximum loss of up to 68%. In juveniles, observed mortality and cannibalism were 10–20% and 6.7–30.0%, respectively, and the maximum loss recorded was 50%. In sub-adults, mortality ranged from 2.8 to 6.7%, cannibalism was up to 20% and the total loss was up to 26.7%. Sub-adults and adults were found to be more tolerant to the infection as evidenced by the mortality pattern. A nested (two-step) PCR resulted in a 570-bp product specific to WSSV in all stages, except the eggs. 相似文献
420.
Kuchijirosho is a fatal disease of commercially cultured fugu, Takifugu rubripes. The transmissible nature of kuchijirosho strongly suggests that an infectious pathogen is the causative agent. Because it is filtrable, the agent is thought to be a virus; however, it has not yet been identified. The lack of a permissive cell line for the putative kuchijirosho-causing agent (KCA) has hindered research on the identification of this pathogen. We inoculated brain extract prepared from kuchijirosho-affected fugu onto an established fugu cell line, fugu eye, and observed that cytopathic effect appeared 7 days after inoculation. Injection of the culture medium of infected fugu eye cells into fugu resulted in the onset of kuchijirosho, indicating that fugu eye cells are able to proliferate KCA. An infectious fraction separated by sodium iottalamate density gradient centrifugation showed a density of 1.15 g mL(-1) equivalent to that of KCA derived from affected fugu brain. To determine whether the genome of KCA is RNA or DNA based, nucleotide synthesis inhibitors were applied to inoculated fugu eye cell line to influence the production of KCA. 5-Fluorouracil but not IUdR showed a concentration-dependent inhibition of KCA yield. These results suggest that KCA is an RNA virus. 相似文献