Resistance screening of breeding lines and commercial tomato cultivars for <Emphasis Type="Italic">Meloidogyne incognita</Emphasis> and <Emphasis Type="Italic">M. javanica</Emphasis> populations (Nematoda) from Ethiopia

Soil and root samples were collected from major tomato growing areas of Ethiopia during the 2012/2013 growing season to identify root-knot nematode problems. DNA-based and isozyme techniques revealed that Meloidogyne incognita and M. javanica were the predominant Meloidogyne species across the sampled areas. The aggressiveness of different populations of these species was assessed on tomato cultivars Marmande and Moneymaker. The two most aggressive populations of each species were selected and further tested on 33 tomato genotypes. The resistance screening and mechanism of resistance was performed after inoculation with 100 freshly hatched (<24 h) second-stage juveniles (J2). Eight weeks after inoculation the number of egg masses produced on each cultivar was assessed. For the resistance mechanism study, J2 penetration and their subsequent development inside the tomato roots were examined at 1, 2, 4 and 6 weeks after inoculation. On both cultivars Marmande and Moneymaker all M. incognita and M. javanica populations formed a high number of egg masses indicating highly aggressive behaviour. Populations from ‘Jittu’ and ‘Babile’ for M. incognita and ‘Jittu’ and ‘Koka’ for M. javanica were selected as most aggressive. None of the 33 tomato genotypes were immune for these M. incognita and M. javanica populations. However, several tomato genotypes were found to have a significant effect on the number of egg masses produced indicating possible resistance. For M. javanica populations there were more plants from cultivars or breeding lines on which no egg masses were found compared to M. incognita populations. The lowest number of egg masses for both populations of M. incognita was produced on cultivars Bridget40, Galilea, and Irma while for M. javanica it was on Assila, Eden, Galilea, Tisey, CLN-2366A, CLN-2366B and CLN-2366C. Tomato genotypes, time (weeks after inoculation) and their interaction were significant sources of variation for J2 penetration and their subsequent development inside the tomato roots. Differential penetration was found in breeding lines such as CLN-2366A, CLN-2366B and CLN-2366C, but many of the selected tomato genotypes resistance for the tested M. incognita and M. javanica populations were expressed by delayed nematode development. Therefore, developing a simple screening technique to be used by local farmers or extension workers is crucial to facilitate selection of a suitable cultivar.     

Regional differences in aspen (Populus tremuloides Michx.) seedling response to an established nursery protocol

New Forests - In seedling-based reforestation operations, seed source is known to be an influential variable affecting outplanting success. Adaptive variation among seed sources may also be an...     

Modeling of the Contour Water Flooding Technology in Microcosms

Eurasian Soil Science - The results are presented of modeling the “contour water flooding” biotechnology aimed at cleaning heavily petroleum-polluted agrosoddy-podzolic soils (Albic...     

Isotopic Composition of Carbon in Humus Acids of Albic Retisols and Luvic Chernozems

Eurasian Soil Science - Carbon isotope ratios in soil organic matter are often used in paleoecological research for reconstructing climate and plant communities (type of photosynthesis). The...     

Relationships between the Size of Aggregates,Particulate Organic Matter Content,and Decomposition of Plant Residues in Soil

Eurasian Soil Science - The distribution of total organic matter (Corg), particulate organic matter (CPOM), and potentially mineralizable organic matter (C0) in mega- (10–5 and 5–2 mm),...     

Assessment of the Impact of No-Till and Conventional Tillage Technologies on the Microbiome of Southern Agrochernozems

Eurasian Soil Science - Quantitative characteristics of microbial communities in southern agrochernozems of the Stavropol region managed with the use of no-till technology and moldboard plowing...     

Water Stability of Soil Aggregates in a 50-Year-Old Soil Formation Experiment on Calcareous Glacial Till

Eurasian Soil Science - Soil formation on the human time scale is immensely time consuming, although it can be significantly accelerated through the effects of vegetation. The content of...     

Soil-Sedimentary Record of the Holocene Environment. Tell Yunacite,Bulgaria

Eurasian Soil Science - A method of the Holocene paleoenvironment reconstruction based on the analysis of pedobiomarkers in tell deposits is suggested. On the Yunacite tell, such a reconstruction...     

Application of Portfolio Theory for the optimal choice of dairy veterinary management programs

Portfolio theory, an established technique in the selection of stocks and financial instruments for investment purposes, was used as a means of selecting an economic mix of veterinary interventions in a dairy herd. The expected return on investment in six veterinary services and the associated standard deviations (as measures of risk) were calculated using computer simulation modeling techniques. Mathematical programming techniques were applied to identify the optimal combinations of veterinary interventions which maximize expected financial returns while minimizing risk. Parametric analysis was used to show how risk (standard deviation) increases as the required expected return is increased along the efficient risk-return frontier of these optimal combinations. This exercise emphasizes the importance of regarding risk in addition to expected return when considering investment in veterinary services.     

Concentrations of bioactive and immunoactive luteinizing hormone in bovine anterior pituitary tissue

Tissue concentrations of LH as determined by radioimmunoassay (RIA) may not accurately measure actual changes that could occur in biological activity of the hormone. To examine this possibility, pituitary homogenates from 135 beef cows in various physiological states were analyzed for content of LH by both RIA and an in vitro bioassay. The ratio of biological/immunological active concentrations of LH remained constant (.52 +/- .02) even though tissue concentrations of immunoactive LH differed among groups. Tissue concentrations of bioactive LH were linearly related to, and highly correlated with (P less than .001), tissue concentrations of immunoactive LH. These data indicate that only a fraction of the immunoactive LH in the bovine pituitary is biologically active. However, this fraction does not vary with the reproductive status or plane of nutrition.