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71.
Capelli G Nardelli S di Regalbono AF Scala A Pietrobelli M 《Veterinary parasitology》2004,123(3-4):143-148
Risk factors associated with Neospora caninum seroprevalence in north-eastern Italy in healthy dogs were assessed. Antibodies to N. caninum were found in 10.9% of 707 kennel and owned dogs by a commercial competitive ELISA (VMRD Inc.). All dogs were negative for Leishmania infantum by indirect fluorescent antibody test indicating no cross reactivity or association between the two protozoa in this area. Seroprevalence association with breed and age of dogs and other factors are discussed. 相似文献
72.
Salvatore Guarino Ezio Peri Paolo Lo Bue Maria Pia Germanà Stefano Colazza Leonid Anshelevich Uzi Ravid Victoria Soroker 《Phytoparasitica》2013,41(1):79-88
The red palm weevil (RPW), Rhynchophorus ferrugineus Olivier (Coleoptera, Curculionidae), is one of the most severe pests of ornamental palm species in urban areas of Mediterranean countries. Aiming to discover inhibitory semiochemicals for RPW population management in urban environments, we conducted electroantennographic (EAG) screenings of 17 commercially available synthetic compounds, representing three groups of plant volatiles (isoprenoids, phenyl propanoid derivatives and fatty acid derivatives) known for their repellent effects toward insects. These tests were followed by trap-based screenings of EAG-active menthone, α-pinene and methyl salicylate, singly and in combination, under urban conditions. In EAG bioassays, RPW antennae of both sexes showed positive dose-dependent responses to 13 of the 17 synthetic chemicals with significant differences among them. In field trapping experiments, conducted in the city of Palermo, Italy, from weeks 31 to 38 in 2010 and 2011, α-pinene, tested singly or in combination with methyl salicylate (2010) or menthone (2011), reduced trap catches by about 30% to 40%. Methyl salicylate and menthone alone were inactive. In conclusion, RPW is significantly affected by α-pinene. This isoprenoid is a promising disruptant for semiochemical-based management of this pest. In addition, identification of a large number of EAG-active chemicals could serve as a database for future design of active repellents or attractants of RPW adults. 相似文献
73.
Vittori Antisari Livia Bianchini Gianluca Cremonini Stefano Di Giuseppe Dario Falsone Gloria Marchesini Marco Marvelli Silvia Vianello Gilmo 《Journal of Soils and Sediments》2016,16(2):645-662
Journal of Soils and Sediments - This study investigated a Lateglacial to Holocene sedimentary sequence derived from a small catchment located at San Lazzaro di Savena in the surroundings of... 相似文献
74.
De Stefano C Milea D Porcino N Sammartano S 《Journal of agricultural and food chemistry》2006,54(4):1459-1466
As a contribution to understanding the speciation of mercury in the environment and to the study of the sequestering ability of phytate (Phy) toward heavy metal and organometal cations, this paper describes the results of an investigation (at t = 25 degrees C by potentiometry, ISE-H+ glass electrode) of its interactions with mercury(II) cation in NaCl aqueous solutions at different ionic strengths (I = 0.15 and 1.0 mol L(-1)), in the pH range 2.5 < or = pH < or = 9.5 and considering metal-to-ligand ratios of 1:1 < or = Hg/Phy < or = 4:1. The formation of 11 HgiHjPhy(12-2i-j)(- species with i = 1 and 0 < or = j < or = 7 and i = 2 and 0 < or = j < or = 2 was observed. Their complex formation constant values proved to be fairly dependent on ionic strength. The speciation of phytic acid and mercury(II) is also dependent on the metal-to-ligand ratio; the dependence of the stability of phytate-mercury(II) species on the phytate protonation step was modeled, and an empirical predictive relationship was proposed. From the results obtained, phytate has very good sequestering ability toward Hg2+, even in the presence of considerable excesses of chloride ion, that is, another ligand strongly interacting with mercury; this supports future studies both on the use of plants that naturally synthesize it for phytoremediation purposes and on its direct application in remediation techniques. 相似文献
75.
Comazzi S Gelain ME Spagnolo V Riondato F Guglielmino R Sartorelli P 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):47-54
BACKGROUND: In dogs, flow cytometry is used in the phenotyping of immunologic cells and in the diagnosis of hemic neoplasia. However, the paucity of specific antibodies for myeloid cells and B lymphocytes and of labeled antibodies for multicolor techniques limits the ability to detect all leukocyte subpopulations. This is especially true for neoplastic and precursor cells. CD18 and CD45 are expressed on all leukocytes and are involved in cell activation, and together could be useful in helping determine cell lineage. OBJECTIVES: The purpose of this study was to double label canine blood for CD18 and CD45 and to use the differential expression of antigens to identify leukocyte populations in dogs with non-neoplastic and neoplastic hematologic diseases. METHODS: A template was developed using blood samples from 10 clinically healthy dogs and a back-gating technique. Differential leukocyte counts obtained with the template were compared with those obtained by manual and automated methods on blood samples from 17 additional healthy dogs. Blood samples obtained from 9 dogs with non-neoplastic (reactive) hematologic diseases and 27 dogs with hemic neoplasia were double stained for CD18 and CD45 using mouse anticanine CD18 monoclonal antibody (mAb) plus phycoerythrin-conjugated rat anticanine CD45 mAb and fluorescein isothiocyanate-conjugated rabbit antimouse IgG. Hemic neoplasms were diagnosed by cell morphology, and immunophenotypic and cytochemical markers. RESULTS: With the double label, neutrophils, eosinophils, monocytes, and T- and B-lymphocytes were identified. In reactive disorders, a population of activated neutrophils with high CD45 and CD18 expression was detected. In hemic neoplasia, cell lineage was easily determined, even in acute leukemia. CONCLUSIONS: Double labeling for CD18/CD45 may be useful as a screening method to evaluate hematologic diseases and help determine cell lineage, and to aid in the selection of a panel of antibodies that would be useful for further analysis. 相似文献
76.
Chiara Bertora Laura Zavattaro Dario Sacco Stefano Monaco Carlo Grignani 《European Journal of Agronomy》2009,30(3):177-186
Soil organic matter (SOM) produces positive effects on multiple soil properties. Increasing its level also provides an opportunity to reduce atmospheric concentration of carbon dioxide (CO2). Recycling animal manure and returning crop residue are among the main practices to enhance organic carbon (C) stock in arable croplands. This study analysed data of soil organic carbon (SOC) stocks from a medium-term field trial (established in 1992) through a Hénin–Dupuis-based equation to determine the proportion of different organic materials retained in the soil as SOM.The treatments included in the experiment simulated different typical management techniques and implied the application to the soil of various organic materials, i.e. cattle slurry, cattle farmyard manure, maize straw, root, maize and ley stubble. Soil organic carbon and total nitrogen (N) (Kjeldhal N) were measured in the 0–30 cm layer during spring 1999 and spring 2003. The relationship between SOC and N stocks measured in 1999 and 2003 and the annual additions of C and N was described by the Hénin–Dupuis-based equation, assuming the presence of two C and N pools (stable and fresh).Our results showed that the application of farmyard manure, slurry and maize straw induced higher C and N content compared to the application of urea without return of crop residue. The different levels of urea application did not produce any significant difference in C and N soil content.We found that approximately 2% of SOC is lost to the atmosphere annually. Furthermore, the amount of C and N retained in the soil each year varied by organic materials: 46% C and 44% N with farmyard manure, 26% C and 11% N with slurry, 28% C and 10% N with root and 6% C with maize straw and ley stubble.These results were used to inform an estimate of C lost or removed to/from atmosphere following manure application and crop residue return for the compilation of greenhouse gas inventories. We found that the most C conservative management is the production and spreading of farmyard manure and that increasing amounts of mineral N fertilizer did not affect the C sequestration extent. 相似文献
77.
Chalinulasterol (1) a new chlorinated sterol disulfate was isolated from the Caribbean sponge Chalinula molitba. Its structure was elucidated using mass spectrometry and NMR experiments. The possible role of chalinulasterol as modulator of the PXR nuclear receptor was investigated but, in spite of the close structural relationship with the PXR agonist solomonsterol A (2), it showed no activity. The structural requirements for the PXR nuclear receptor activity were discussed. 相似文献
78.
The global seafood industry, influenced by consumer demand, is closely linked to the global fishing industry, which determines the variety of fish available for consumption. The recently revealed issue of seafood mislabelling threatens to weaken this link by removing consumer power to influence patterns of fisheries exploitation through informed choice. Recognizing this, there is an urgent need to go beyond the mere documentation of the phenomenon and learn more about the origins of this problem and the nature of factors influencing its occurrence to develop solutions. In an attempt to understand seafood mislabelling more thoroughly in Europe, 226 cod products were purchased from Ireland and the UK, genetically identified using a DNA barcoding technique (COI barcoding gene), and species identification results were compared against product labels. Cod mislabelling proved more severe in Ireland than in the UK (28.4% vs. 7.4%). Moreover, whereas data show that in Ireland, cheaper species are sold as cod, in the UK, threatened Atlantic cod (Gadus morhua) may be sold as ‘sustainably sourced’ Pacific cod. Considering these countries operate under the same EU policies for seafood traceability and labelling, it is likely that this situation has been influenced by heightened consumer awareness in the UK, which has created an environment where mislabelling is discouraged. In addition to identifying samples, traceability information from packaged cod was used to trace products back to supplying companies. Although inconclusive in determining blame, this exercise has demonstrated that using traceability information can add explanatory power when attempting to determine responsibility for the occurrence of mislabelling. 相似文献
79.
Annual survival probabilities of juvenile loggerhead sea turtles indicate high anthropogenic impact on Mediterranean populations 下载免费PDF全文
Paolo Casale Daniela Freggi Giovanni Furii Carola Vallini Pasquale Salvemini Michele Deflorio Giovanni Totaro Stefano Raimondi Caterina Fortuna Brendan J. Godley 《水产资源保护:海洋与淡水生态系统》2015,25(5):690-700
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80.
IMAGING DIAGNOSIS—ULTRASONOGRAPHIC APPEARANCE OF SKELETAL MUSCLE METASTASES IN A DOG WITH HEMANGIOSARCOMA 下载免费PDF全文
Martina Fabbi Stefano Di Palma Sabrina Manfredi Giacomo Gnudi Francesca Miduri Eleonora Daga Gabriele Costantino Melis Ezio Bianchi Simone Voccia Antonella Volta 《Veterinary radiology & ultrasound》2017,58(6):E64-E67
A 13‐year‐old spayed female German shepherd dog was presented for acute onset of lethargy, anorexia, and disseminated erythematous skin lesions. Thoracic radiographs and abdominal ultrasonographic findings were consistent with metastatic hemangiosarcoma. Multiple, ill‐defined, irregularly shaped hypoechoic nodules were also detected within the thoracic and abdominal wall. Hemangiosarcoma metastases to the skeletal muscle were confirmed based on histopathological examination. Multivisceral involvement was also confirmed by necropsy. Metastatic neoplasia should be considered as a differential diagnosis for dogs with ill‐defined, irregular, hypoechoic, intramuscular nodules. 相似文献