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11.
Alana Batista dos Santos Mara Lúcia Albuquerque Pereira Herymá Giovane de Oliveira Silva Gleidson Giordano Pinto de Carvalho Taiala Cristina de Jesus Pereira Leandro Sampaio Oliveira Ribeiro José Augusto Gomes Azevêdo Maria das Graças Conceição Parada Costa Silva Larisse Borges Sousa Leandro Borges Sousa Daiane de Oliveira Alencar 《Tropical animal health and production》2016,48(3):509-515
12.
Aguiar PH Barrouin-Melo SM Franke CR dos Santos RR Silva TM Mengel JO dos-Santos WL Pontes-de-Carvalho L 《Veterinary journal (London, England : 1997)》2007,173(1):158-166
This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of immune functions in dogs. 相似文献
13.
Stefan Möth Markus Redl Silvia Winter Florian Hüttner Siegrid Steinkellner 《Pest management science》2023,79(9):3080-3089
BACKGROUND
A reduction in chasmothecia, an important inoculum of grape powdery mildew (Erysiphe necator Schwein.), is essential for disease control in vineyards; the use of fungicides during the formation of chasmothecia on vine leaves, late in the growing season, may accomplish this. Inorganic fungicides, such as sulphur, copper, and potassium bicarbonate, are very useful for this purpose because of their multisite mode of action. The aim of this study was to evaluate chasmothecia reduction using different fungicide applications late in the growing season in commercially managed vineyards and in an exact application trial.RESULTS
Chasmothecia on vine leaves were reduced in commercial vineyards by four copper (P = 0.01) and five potassium bicarbonate (P = 0.026) applications. The positive effect of potassium bicarbonate was also confirmed in the application trial, where two applications showed lower chasmothecia numbers than the control (P = 0.002).CONCLUSION
The application of inorganic fungicides reduced the amount of chasmothecia as the primary inoculum source. Potassium bicarbonate and copper are of further interest for disease control as these fungicides can be used by organic and conventional wine growers. The application of these fungicides should be carried out as late as possible before harvest to reduce chasmothecia formation and, consequently, the potential for powdery mildew infestation in the subsequent season. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献14.
15.
16.
An understanding of seasonal dynamics in the free-living phase of the tropical horse tick, Anocentor nitens, is vital for effective control measures. This study examines seasonal changes in the oviposition and incubation periods, eclosion rates, reproductive success, and larval survival in the free-living phase during the period June 1995-July 1997. Ten engorged female ticks, placed individually in wire-gauze tubes, were exposed in a field of Brachiaria decumbens fortnightly each month, and monitored to measure the duration of the various reproductive parameters, and success. Larval abundance was estimated indirectly, through the percentage of females with viable progeny, and directly by using a flagging method. Duration of the free-living cycle ranged from 12.3 weeks (October and December) to 23.5 weeks (March). The cycle was prolonged in cold and dry months. Larval survival was longer from February to July, and reduced from August to February. Oviposition was recorded in all months, and the egg production index ranged from 26.7 to 47.1%. The eclosion rate ranged from 0.1 to 76.6% in different months. Monthly estimates of larval abundance in the pasture were similar for the two methods used, being higher in months with higher temperatures, relative humidity and rainfall. 相似文献
17.
The induction of endometrial prostaglandin (PG) F2 synthesis by oxytocin is dependent upon activation of phospholipase (PL) A2 and mobilization of arachidonic acid. The objective of this study was to determine if oxytocin stimulates PGF2 synthesis by inducing synthesis of cytosolic PLA2 (cPLA2). In Experiment 1, 15 ovariectomized ewes were given progesterone and estradiol to simulate an estrous cycle. Ewes were then given an injection of oxytocin on Day 14 of the simulated estrous cycle. Jugular blood samples were collected and assayed for 13,14-dihydro-15-keto-prostaglandin F2 (PGFM). Uteri were collected at 0, 7.5, 25, 90, or 240 min postinjection (n = 3 ewes/time point). Total RNA was isolated from caruncular endometrium and subjected to dot-blot analysis. Oxytocin induced a rapid and transient increase in serum PGFM (P < 0.01). However, endometrial concentrations of cPLA2 mRNA did not change following oxytocin administration (P > 0.10). In Experiment 2, 11 ovary-intact ewes were given oxytocin (n = 5) or saline (n = 6) on Day 15 after estrus. Jugular blood samples were collected and assayed for serum concentrations of PGFM. Uteri were collected at 15 min postinjection. Homogenates were prepared from caruncular endometrium and subjected to Western blot analysis. Concentrations of PGFM were higher in oxytocin treated ewes compared to saline treated ewes at 15 min postinjection (P < 0.01). Endometrial concentrations of cPLA2 protein were greater in the cytosolic than in the microsomal fraction (P < 0.01). Oxytocin did not affect the amount of cPLA2 protein in either fraction (P > 0.10). In conclusion, oxytocin did not effect expression of either cPLA2 mRNA or protein in ovine endometrium. Oxytocin may stimulate PGF2 synthesis by activating cPLA2 protein that is already present in an inactive form. 相似文献
18.
In order to offer hints for new research, the agronomic techniques used to manageOrobanche crenata in Sicily in the late 19
th
and early 20
th
Century are examined; the publications in which they were originally described can be obtained only with difficulty. Hand
pulling, resistant cultivars and late sowing are the techniques used nowadays. Screening for resistance to broomrape of the
traditional cultivars of broad bean, and for the influence of sowing density on broomrape infestations, is still to be done. 相似文献
19.
Silvia Ribeiro de Souza e Silva Luiz Claúdio de Almeida Barbosa Beatriz King-Diaz Blas Lotina-Hennsen 《Pesticide biochemistry and physiology》2007,87(2):109-114
3,4-Seco-friedelan-3-oic acid was isolated from Maytenus imbricata (Celastraceae). At low concentrations it inhibited non-cyclic electron transport and ATP synthesis in spinach chloroplasts, i.e., it behaved as a Hill reaction inhibitor, and at high concentrations it acts as an uncoupler by enhancing uncoupled electron transport and Mg2+-ATPase activity. 3,4-Seco-friedelan-3-oic acid did not inhibit PSII electron transport from DPC to DCPIPox and photosystem I activity, but it enhanced from TMQH2 to MV, corroborating its action as uncoupler. It inhibits electron flow through PSII from water to sodium silicomolybdate. The whole results indicate that the 3,4-seco-friedelan-3-oic acid target is at the OEC complex enzyme, the donor side of PSII. The fluorescence decay data shows the formation of the K-band, which match this result, acting as inhibitor at the donor side of PSII and it as an uncoupler. 相似文献
20.
Balboni Andrea Terrusi Alessia Urbani Lorenza Troia Roberta Stefanelli Silvia A. M. Giunti Massimo Battilani Mara 《Veterinary research communications》2022,46(1):223-232
Veterinary Research Communications - Canine parvovirus type 2 (CPV-2) is one of the most relevant pathogens associated with enteritis in dogs and is frequently reported in association with the... 相似文献