Molecular cloning and functional analysis of duck Toll-like receptor 5

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-κB-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog.     

水稻雄性不育突变体012S-3的遗传分析和基因定位

雄性不育是水稻杂种优势利用的重要资源,对雄性不育现象的研究具有重要理论意义和实践价值。本研究以自然突变的水稻雄性不育突变体012S-3为试验材料,对其表型特征和花粉育性等进行调查,并构建遗传群体,利用分子标记对目的基因进行初步定位,然后应用基因组重测序技术对其进行精细定位。结果表明,012S-3是一个典型的无花粉普通型雄性不育材料,其不育性状受1对隐性核基因控制。初步定位分析目的基因与SSR标记RM6081存在连锁关系,其遗传距离约为34.4 c M;进一步的精细定位分析,找到3个候选基因:LOC_Os07g35880、LOC_Os07g35920和LOC_Os07g35940,其中LOC_Os07g35880和LOC_Os07g35940编码β-淀粉酶,属于水稻中新发现的花粉致死基因。该不育基因的成功定位为其进一步的分离克隆及其在水稻分子设计育种中的应用奠定了基础。     

Hydraulic Retention Time Influence on Improving Flocculation in the Activated Sludge Processes Through Polyelectrolytes

Hydraulic retention time (HRT) influence improving sludge flocculation with adding the polyelectrolytes (non-ionic, anionic, and cationic) was studied on an activated sludge (AS) system fed with synthetic domestic wastewater (SDW), dairy industry wastewater (DIW), and caramel industry wastewater (CIW). The sludge volumetric index, food/microorganism ratio (F/M), and mixed liquor volatile suspended solids at different HRTs (6, 8 and 10 h) were monitored on an experimental model. Results showed that both SDW and IW had the best sludge flocculation conditions at 8 h and 100 mL of non-ionic polyelectrolyte (0.2 mg L^?1). In addition, this phenomenon reached the organic matter removal efficiencies of 95.9, 95.7, and 94.2% for SDW, DIW, and CIW, respectively. Therefore, optimum HRT increased the organic matter removal efficiencies by 10%, sludge concentration by 37% (22–55%), and F/M ratio by 70%. Moreover, the polyelectrolytes used in AS improved the sludge flocculation by 2.9 times.     

水稻恶苗病病原菌的研究

 1977~1987年采集103个稻恶苗病标本,分离后获8种镰刀菌。其中串珠镰孢(Fusarium moniliforme)的一个变种占总标本数的60.2%,其特征为:菌落生长直径2-4cm;大型分生孢子镰刀形,3-5隔;小型分生孢子卵形,串生和假头生;产孢细胞单、复粳并存;具厚垣孢子,球至亚球形。此变种定名为(F. moniliforme var. Zhejiangensis Wang et Chen var. nov.)。其他7种镰刀菌为木贼镰孢(F. equiseti),禾谷镰孢(F. graminearum),砖红镰孢(F. lateritium),雪腐镰孢(F. nivale),尖镰孢(F. oxysporum),半裸镰孢(F. semitectum),腐皮镰孢(F. solani)。试验证明,串珠镰孢浙江变种是水稻恶苗病的主要致病菌;其他6种(除腐皮镰孢未接种外)均有不同程度的弱致病性。串珠镰孢浙江变种也能侵染小小麦、大麦、玉米、西瓜、豌豆等作物的幼苗,并有徒长症状。     

猪结合珠蛋白基因的克隆、原核表达及单克隆抗体制备

本研究的目的在于利用重组猪结合珠蛋白(Haptoglobin,Hp)制备特异性单克隆抗体.以梅山猪肝脏总RNA为模板,利用RT-PCR方法扩增猪hp基因,将其克隆到原核表达栽体pGEX-KG和pET-32a上,转化至E.coli BL21中,IPTG诱导下高效表达重组GST-Hp和HIS-Hp蛋白.用纯化的重组GST-Hp免疫BALB/c小鼠,用纯化的HIS-Hp作为包被抗原进行间接ELISA检测,通过淋巴细胞杂交瘤技术筛选阳性细胞株,制备单克隆抗体.结果表明筛选到2株高效分泌抗猪Hp单克隆抗体的杂交瘤细胞株,命名为3B4和3C8.Western-blot和间接ELISA检测均表明制备的单克隆抗体具有良好的特异性.     

北京市怀柔区设施种植业废弃物处理现状及发展

针对北京市怀柔区设施种植业废弃物处理的现状,分析废弃物处理过程中存在的问题,并提出相应的解决措施。