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41.
Sarkaki A Amani R Badavi M Moghaddam AZ Aligholi H Safahani M Haghighizadeh MH 《Pakistan journal of biological sciences: PJBS》2008,11(8):1114-1119
The aim of this study was to evaluate the effects of different doses of dietary soy meals (with or without isoflavone) on dementia in ovariectomized (OVX) animal model of Alzheimer's disease. Female Wistar's rats with the exception of intact group were ovariectomized at the first line of study. Animals were divided into 2 main groups: control (c) and pre-treatment groups. Animals in pre-treatment groups received one of five types of diet during four weeks prior Nucleus Basalis Magnocellularis (NBM) electrical lesion normal diet (0), 10 g soy with isoflavone (10), 20 g soy with isoflavone (20), 10 g soy without isoflavone (-10) and 20 g soy without isoflavone (-20) in 30 g daily diet. The spatial learning and memory were tested using Morris water maze after electrical lesion. Rats were trained in water maze to find a hidden escape Platform. Rats received 6 blocks that each block consisted of 3 trials. Following acquisition trials, one probe trial was conducted in which the platform was removed. Soy meal diet (with or without isoflavone) in ovariectomized rats with Alzheimer's disease caused improvement of performance across 18 trials of Acquisition. Our results suggest that soy meal is a potential alternative to estrogen in the prevention and treatment of Alzheimer's disease. 相似文献
42.
Fatemeh Morovvat Seyed Ziae Aldin Samsam Shariat Maryam Davoudi Dariush Norouzian 《Iranian Biomedical Journal》2022,26(2):132
Background:Immobilization is an approach in industry to improve stability and reusability of urease. The efficiency of this technique depends on the type of membrane and the method of stabilization. Methods:The PEI-modified egg shell membrane was used to immobilize urease by absorption and glutaraldehyde cross-linking methods. The membranes were characterized by FTIR and AFM, and Nessler method was applied to measure the kinetic of the immobilized enzymes. Finally, the storage stability (6 °C for 21 days) and reusability (until enzyme activity reached to zero) of the immobilized enzymes were investigated. Results:Based on FTIR, three new peaks were observed in both the absorption- (at 1389.7, 1230.8, and 1074.2 cm-1) and the cross-linking (at 1615-1690, 1392.7, 1450 cm-1) immobilized enzymes. The surface roughness of the native membrane was altered after PEI treatment and enzyme immobilization. The optimal pH of cross-linking immobilized enzymes was shifted to a more neutral pH, while it was alkaline in adsorption-immobilized and free enzymes. The reaction time decreased in all immobilized enzymes (100 min for free enzyme vs. 60 and 30 min after immobilizing by adsorption and cross-linking methods, respectively). The optimal temperature for all enzymes was 70 °C and they had a higher Km and a lower Vmax than free enzyme. The stability and reusability of urease were improved by both methods. Conclusion:Our findings propose these approaches as promising ways to enhance the urease efficiency for its applications in industries and medicines. Key Words: Egg shell, Immobilization, Polyethylenimine, Urease 相似文献
43.
44.
Pomegranate fruit is an important source of potentially healthy bioactive compounds and mineral nutrients. Changes in total phenolic compound, concentrations, and levels of macronutrients (P, K, N, Mg, Ca and Na) and micronutrients (Zn, Cu, Mn, Fe and B) in arils and peel of pomegranate fruit were recorded from 10 days after full bloom until harvest. Total phenolics levels increased at early stage of growth both in peel and arils of fruit, but thereafter generally decreased during maturation and reached to 3.70 and 50.22 mg g−1 of dry weight in arils and peel, respectively, at harvest. The amount of total phenolics in peel was markedly higher than arils of pomegranate fruit. The concentration of most elements in arils and peel decreased during fruit growth and development. At harvest the relative order of concentration of macronutrients both in arils and peel was K > N > Ca > P > Mg > Na. The concentration of most micronutrients was greater in the arils than in the peel especially in early season. The relative order of concentration of micronutrients in arils was B > Fe > Zn > Cu > Mn. The accumulation of all the macro- and microelement within the fruit also increased during fruit growth and development. These results provide important data on total phenolics and macro- and micronutrient changes during fruit growth and development, emphasizing that pomegranate fruit can be a good source of bioactive compounds and minerals. 相似文献
45.
Abdolkarim Zarei Zabihollah Zamani Reza Fattahi Alireza Salami Amir Mousavi 《International Journal of Fruit Science》2016,16(3):242-258
To find out more about the biochemical aspects of seed-softness trait in pomegranate (Punica granatum L.), some of the phenylpropanoid pathway enzymes as well as their products were evaluated in the various soft- and hard-seeded genotypes during fruit development. Results showed that peroxidase (POD) activity decreased in all studied genotypes during fruit growth, while increasing at the last stages only in the hard-seeded genotypes. Although laccase (LAC) activity did not show high differences during fruit ripening and in different genotypes, but its general trend was increasing. Phenylalanine ammonia-lyase (PAL) activity decreased during the fruit development and approximately diminished for the hard-seeded genotypes. All genotypes had lignin components in their seeds and amount of lignin increased during the growth season. Total phenolic decreased drastically and antioxidant capacity increased gradually showing an opposite trend and probable competitive effect for the same substrate during fruit development. Considerable differences that were observed in the POD activity at the last sampling stage, and higher activity that was observed in POD than LAC, may indicate that POD is more important for polymerization of lignin in the pomegranate seed and this enzyme might have some role in the development of seed softness trait in pomegranate. Also, this is the first report about lignin detection in the seeds of soft-seeded pomegranate genotypes, and represents a different mechanism (such as monolignol conformation in the final polymer) from lignin content for development of the soft-seeded pomegranate genotype. 相似文献
46.
Muhammad Iqbal Alireza Navabi Donald F. Salmon Rong-Cai Yang Brenda M. Murdoch Steve S. Moore Dean Spaner 《Euphytica》2007,154(1-2):207-218
Due to the short growing season in the high northern latitudes, the development of early maturing spring wheat (Triticum aestivum L.) cultivars is important to avoid frost damage which can lower production and quality. We investigated earliness of flowering and maturity, and some associated agronomic traits, using a set of randomly selected high northern latitude adapted spring wheat cultivars (differing in maturity) and their F1 and F2 crosses made in a one-way diallel mating design. The parents, and their F1 and F2 crosses were evaluated under field conditions over 2 years. Anthesis and maturity times were controlled by both vernalization response and earliness per se genes, mainly acting additively. Non-additive genetic effects were more important in controlling grain fill duration, grain yield and plant height. Additive × additive epistatic effects were detected for all traits studied except time to anthesis. Segregation analyses of the F2 populations for time to anthesis indicated the presence of different vernalization response genes. Molecular genetic analyses revealed the presence of Vrn-A1 and Vrn-B1 genes in the parental cultivars. Narrow-sense heritability was medium to high (60–86%) for anthesis and maturity times but low to medium (13–55%) for grain fill duration, plant height and grain yield. Selection for early flowering/maturity in early segregating generations would be expected to result in genetic improvement towards earliness in high latitude spring wheats. Incorporation of the vernalization responsive gene Vrn-B1 in combination with vernalization non-responsive gene Vrn-A1 into spring wheats would aid in the development of early maturing cultivars with high grain yield potential for the high latitude wheat growing regions of the northern hemisphere. 相似文献
47.
Mohammad Reza Naghavi Mohsen Mardi Seyed Mostafa Pirseyedi Mehrbanu Kazemi Payam Potki Mohammad Reza Ghaffari 《Genetic Resources and Crop Evolution》2007,54(2):237-240
Genetic diversity of 54 accessions of Aegilops tauschii from five countries was assessed using sequence-tagged microsatellites (or simple sequence repeats, SSRs) and amplified fragment
length polymorphisms (AFLPs). In the case of AFLP analysis, a total of 256 amplification products obtained, 234 of them were
polymorphic across all the 54 accessions. A total of 224 fragments were obtained from the 24 SSR primers and 219 of fragments
were polymorphic across all the genotypes screened. Based on both AFLP and SSR markers, the highest percentage of polymorphisms
were obtained in Iranian and accessions of unknown origin. The highest polymorphic information content (PIC) value was observed
for SSRs (0.82) while the highest marker index (MI) value was for AFLPs (8.5) reflecting the hyper-variability of the first
and the distinctive nature of the second system. Principal co-ordinate analysis (PCO) revealed congruent patterns of genetic
relationships for both data sets, but did not group accessions strictly according to their geographical origins. Poor correlation
was found between AFLP and SSR marker loci. This low association may be due to low number of AFLP and SSR markers. These results
show that molecular markers can help to organize the genetic variability and expose useful diversity for breeding purposes. 相似文献
48.
Raf kinases have been linked to endothelial cell survival. Here, we show that basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) differentially activate Raf, resulting in protection from distinct pathways of apoptosis in human endothelial cells and chick embryo vasculature. bFGF activated Raf-1 via p21-activated protein kinase-1 (PAK-1) phosphorylation of serines 338 and 339, resulting in Raf-1 mitochondrial translocation and endothelial cell protection from the intrinsic pathway of apoptosis, independent of the mitogen-activated protein kinase kinase-1 (MEK1). In contrast, VEGF activated Raf-1 via Src kinase, leading to phosphorylation of tyrosines 340 and 341 and MEK1-dependent protection from extrinsic-mediated apoptosis. These findings implicate Raf-1 as a pivotal regulator of endothelial cell survival during angiogenesis. 相似文献
49.
Gholam Ali Akbari Mostafa Hojati Seyed Ali Mohammad Modarres-Sanavy Faezeh Ghanati 《Pesticide biochemistry and physiology》2011,100(3):244-250
The ability of hexaconazole (HEX) to ameliorate salinity stress was studied in canola plants (Brassica napus L.). Canola seedlings were subjected to sodium chloride (NaCl) treatment. A treatment with 200 mM NaCl reduced growth parameters, chlorophyll content and protein content as well as increased the proline (Pro) content in canola plants. In addition, NaCl stress increased the endogenous, nonenzymatic antioxidants and the activity of antioxidant enzymes, such as peroxidase (POX; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1) and catalase (CAT; EC 1.11.1.6). When these plants were treated with a combination of NaCl and 50 mg L−1 HEX, the inhibitory effects of NaCl stress were decreased by increasing the root growth, shoot growth, dry weight (DW), chlorophyll content, protein content and antioxidant enzyme activity by ameliorating the salinity injury. These results suggested that HEX has an important role in the enhancement of plant antioxidant systems and resistance to salinity in canola plants. 相似文献
50.
Alireza Koocheki Seyyed Mohammad Seyyedi 《Communications in Soil Science and Plant Analysis》2020,51(9):1161-1183
ABSTRACT Evaluation of the relationships between nutritional patterns and farms’ age with the behavior of saffron (Crocus sativus L.) daughter corms based on farmers’ management (on-farm) can be crucial in improving saffron sustainable yield. Furthermore, in commercial saffron production, especially in small farms, the purchased water and fertilizers are the basis of sustainable saffron profitability, hence, recognizing the relationships between economic water use efficiency (EWUE) and economic fertilizer use efficiency (EFUE) can be important. An on-farm experiment was conducted on a large scale based on farmers’ management in Torbat Heydarieh, Iran. The farms’ age (1–6 years old) and fertilizers management approaches (organic, mineral and integrated) were considered as the first and second factors, respectively. Large-sized daughter corms number and weight and corms N and P content increased with increasing farms’ age from 1 to 4 years old. However, these parameters decreased with increasing farms’ age from 4 to 6 years old. Irrespective of saffron farms’ age, the minimum large-sized daughter corms number and weight were observed when mineral fertilizer management was practiced. By contrast, the maximum values were related to integrated fertilizer management. In each type of fertilizer management, EWUE and EFUE (based on large-sized daughter corms monetary value) increased with increasing farms age from 1 to 4 years. However, increase in farms age from 4 to 6 years caused a significant reduction in mentioned indices. Overall, if high-quality saffron corm production is needed, corms should be harvested in the fourth year to gain the maximum yield and profit. 相似文献