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91.
92.
Ovulation and spawning induction in Caspian kutum Rutilus frisii kutum by administration of GnRHa and catecholaminergic pharmaceutical compounds with Ovaprim
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Sara Koohilai Shahrbanoo Oryan Homayoun Hosseinzadeh Sahafi Pargol Ghavam Mostafavi Safieh Behzadi 《Aquaculture Research》2017,48(4):1469-1477
The acceleration of final oocyte maturation and ovulation is recognized as a principal process in Caspian kutum Rutilus frisii kutum, a commercial and valuable species in Caspian Sea. The aim of this study was to evaluate the effects of catecholaminergic pharmaceutics which include Salbutamol (β2‐adrenergic receptor agonist), Metoprolol (β1‐adrenergic receptor antagonist), Clozapine and Olanzapine (the third generation of D4 and D2 dopamine receptor antagonists) which can be more efficient than the first and second generations, in combination with Buserelin ([D‐Ser(tBu)6,Pro9‐NEt]‐GnRHa) and Ovaprim (D‐Ala6,Pro9‐Net)‐sGnRH+Domperidone on ovulation and spawning induction in Caspian kutum. One hundred eight adult females were injected once with Buserelin Acetate (BUS) 5 μg kg?1 BW, Ovaprim 0.5 mL kg?1 BW, Salbutamol Sulphate (SLB) 4 mg kg?1 BW, Olanzapine (OLZ) 5 mg kg?1 BW, Clozapine (CZ) 12 mg kg?1 BW and Metoprolol Tartrate (MTP) 5 mg kg?1 BW, being divided into 12 groups: group 1, intact (negative control); group 2, Ovaprim (positive control); group 3, OLZ+SLB; group 4, OLZ+MTP; group 5, OLZ+BUS; group 6, CZ+SLB; group 7, CZ+MTP; group 8, CZ+BUS; group 9, OLZ+BUS+SLB; group 10, OLZ+BUS+MTP; group 11, CZ+BUS+SLB and group 12, CZ+BUS+MTP (N = 9). The results showed that the highest mean value in ovulation success, ovulation index, fertilization success, relative fecundity and the number of eggs belonged to Ovaprim treatment. On the other hand, spawning was successful in OLZ+BUS+SLB and CZ+BUS treatments (P < 0.05), whereas it was lower in CZ+MTP, CZ+BUS+MTP and OLZ+BUS than the other treatments. Therefore, it could be concluded that Clozapine and Olanzapine potentiated the effect of Buserelin treatment in ovulation and spawning induction, while Metoprolol blocked the stimulatory effects of GnRHa, Clozapine and Olanzapine. Salbutamol also can amplify stimulatory effect of all mentioned pharmaceutics. 相似文献
93.
- 1. The implications of shrinkage associated with desiccation and ethanol preservation for seahorses (genus Hippocampus) were investigated using Hippocampus guttulatus (European long‐snouted seahorse) as a model. Specifically, this research addressed the implications of preservation for taxonomy and life history studies and the application of minimum size limits (MSL) for managing seahorse trade.
- 2. In 2004, the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) listed all seahorse species on its Appendix II, and recommended a 10 cm MSL as an interim means of ensuring sustainable international trade. Inconsistencies in seahorse measurement methods and repeatability posed challenges for applying the MSL. Moreover, the shrinking effect of desiccation on body length observed in other fish was assumed to be negligible for seahorses because of their high degree of ossification.
- 3. Changes in seahorse sizes were measured following immersion in ethanol and desiccation. H. guttulatus shrank on average by 0.1–2.3% when preserved in ethanol and 3.0–6.4% when dried, depending on the trait measured. Similar trends were observed in a sample of H. kuda (yellow seahorse). Specimen posture during drying, and measurement methods also influenced estimates of size.
- 4. Based on the shrinkage observed, 14–44% of captured seahorses that are dried could shrink to below the recommended MSL, even if all seahorses were longer than the MSL at capture. This demonstrates that small changes in body lengths can have significant implications for trade of species managed with size limits.
- 5. Recommendations are to (1) standardize seahorse measurement methods, (2) consider the effects of preservation and measurement technique on body lengths, and apply appropriate corrections in comparative studies and when developing fisheries management strategies, and (3) adjust size limits at the point of capture to ensure retained seahorses comply with the CITES recommended MSL.
94.
Sharp-crested weirs are the simplest form of over-flow spillway that commonly used to determine the flow rate in hydraulic
laboratories, industry and irrigation systems, where highly accurate discharge measurements are needed. In this study, the
experimental upper and lower nappe profiles in rectangular sharp-crested weirs are fitted by quadratic and cubic equations,
respectively. In addition, free-vortex theory is used to simulate flow over this kind of weirs and determine discharge coefficient.
Physical models of sharp-crested weirs with various widths and heights were considered. The proposed method agrees well with
the experimental observations. Also, the experimental data indicate that the suggested equation presents reasonable results
for the range of 0 < h/P < 9. 相似文献
95.
Bartholomew W Green Peter Perschbacher Gerald Ludwig Sara E Duke 《Aquaculture Research》2010,41(10):e524-e536
Plankton community structure and chlorophyll a concentration were compared in twelve 0.1 ha earthen ponds co‐stocked with channel catfish (Ictalurus punctatus Rafinesque, 1818) in a multiple‐batch culture (initial biomass=5458 kg ha?1) and a planktivore, threadfin shad (Dorosoma petenense Güther, 1867; initial biomass=449 kg ha?1), during the April–November growing season. We used a completely randomized design in a 2 × 2 factorial arrangement to test the planktivore level (presence or absence of threadfin shad) and channel catfish feeding frequency (daily or every third day). Channel catfish were fed a 32% protein feed to apparent satiation on days fed. The presence of threadfin shad affected phytoplankton and zooplankton community structure more than did feeding frequency, and the impact in ponds was more pronounced after 1 July. The numbers of all major groups of zooplankton were lower in ponds with threadfin shad, but were unaffected by the feeding frequency. Chlorophyll a concentration before 1 July was higher in ponds with threadfin shad and unaffected by the feeding frequency, whereas after 1 July it was higher in ponds without threadfin shad and that were fed daily. Phytoplankton community structure after 1 July was dominated by nuisance algal bloom genera of cyanobacteria in ponds without threadfin shad and by Bacillariophyceae in ponds with threadfin shad. 相似文献
96.
Joseph R. Sara Wilmien J. Luus-Powell Susan B. Fogelson Hannes Botha Theresa C. Guillette Willem J. Smit Andre Hoffman Katlego D. Kunutu Jeremy P. Koelmel John A. Bowden 《Journal of fish diseases》2020,43(10):1185-1199
Pansteatitis is the leading cause for the decline in Nile crocodile populations and the sporadic mortality of fish in the Olifants River System, South Africa. To determine the prevalence of this disease in lentic systems, Mozambique tilapia, Oreochromis mossambicus, were collected from Lake Loskop, Lake Flag Boshielo, Phalaborwa Barrage and Lake Luphephe-Nwanedi. The former three impoundments are located within the main stem of the Olifants River, while the latter, which is geographically isolated and situated in the Limpopo River System, served as a reference site. Mesenteric adipose, liver, serosa of the swim bladder, gill and the skeletal muscle of fish sampled were examined for gross and microscopic evidence of pansteatitis. Microscopically observed changes were used to statistically compare pansteatitis prevalence between samples and sites. Based on histopathological evaluation, the adipose tissue in the liver, swim bladder serosa and coelom from severely debilitated individuals showed the most significant pathological changes. Lesions indicative of steatitis were observed in fish collected from Lake Loskop (75%), Lake Flag Boshielo (22%) and Lake Luphephe-Nwanedi (15%). Further investigation is warranted to understand the pervasiveness and mechanisms driving pathological changes of pansteatitis at Lake Flag Boshielo, Phalaborwa Barrage and Lake Luphephe-Nwanedi. 相似文献
97.
Trine Ytrestøyl Harald Takle Jelena Kolarevic Sara Calabrese Gerrit Timmerhaus Bjørn O. Rosseland Hans C. Teien Tom O. Nilsen Sigurd O. Handeland Sigurd O. Stefansson Lars O. E. Ebbesson Bendik F. Terjesen 《Journal of the World Aquaculture Society》2020,51(2):373-392
Producing a larger post-smolt in recirculating aquaculture systems (RAS) could shorten the production time in sea cages and potentially reduce mortality. Knowledge of the biological requirements of post-smolts in closed-containment systems is however lacking. In the present study, the effects of salinity and water velocity on growth, survival, health, and welfare of Atlantic salmon reared in RAS were examined. Salmon smolts were stocked in three separate RAS with salinities of 12, 22, and 32‰ and subjected to high (1.0 body lengths per s−1) or low (0.3 body lengths second−1) water velocity. Growth performance, survival, welfare, and physiological stress responses were monitored until the fish reached a bodyweight of around 450 g. Growth rate was higher at lower salinity and higher water velocity generally had a positive effect on growth in all salinities. Feed conversion ratio was lower at 12‰ compared to the 22 and 32‰ when the fish were between 250 and 450 g. Higher mortality, elevated plasma cortisol levels, higher incidence of cataract, and a higher expression of stress-induced genes in the skin (iNOS, Muc5ac-like) indicated a negative effect of higher salinity on fish welfare. Male maturation was low (<1%), and not affected by salinity or water velocity. 相似文献
98.
Marcia Saraiva Max J. Beckmann Sara Pflaum Marianne Pearson Daniel Carcajona James W. Treasurer Pieter van West 《Journal of fish diseases》2019,42(3):335-343
Samples from moribund lumpfish were collected in a marine hatchery in Scotland in 2015. Black nodules were noted on the skin, and gills and fungal hyphae were extensively distributed in musculature and internal organs. Multifocal chronic inflammatory lesions displaced structures in all affected organs. Mortalities commenced on completion of spawning in May and were evenly distributed over the second year in the temperature range 11–15°C. The main systemic infection causing agent was initially identified based on morphological characteristics as an Exophiala species. Ribosomal DNA (rDNA) ITS regions of the isolates were subsequently sequenced confirming the isolates belonged to Exophiala genus. All isolates fell in a single phylogenetic cluster, which is represented by Exophiala angulospora. Fish were treated with either formalin or Bronopol or a combination of both, but there was no effect on the pattern or numbers of mortalities. Isolates were also tested against three different concentrations of Latrunculin A, Amphotericin B and Itraconazole with no success. It is of utmost importance to increase the knowledge on pathogen–host interactions to successfully develop sustainable control methods. 相似文献
99.
Sara Pourhosein Sarameh Amir Houshang Bahri Bahram Falahatkar Mahtab Yarmohammadi Alireza Salarzadeh 《Aquaculture Nutrition》2019,25(3):543-554
The aim of this study was to evaluate the long‐term effects (7‐month experiment) of diets consisting of fish oil (Kilka fish) and vegetable oil (rapeseed) on the reproductive performance of sterlet sturgeon (Acipenser ruthenus) broodstock. Forty‐five broodstock (990.3 ± 20.05 g) were randomly allocated to three different diet treatments. Three experimental diets were formulated with graded levels of fish oil (100% FO), vegetable oil (100% VO), and a combination of fish and vegetable oil (50% FO + 50% VO). At the end of the 7‐month feeding trial period, the weight gain and final weight were changed significantly different between the treatments (p < 0.05). Broodstock fed the FO + VO diet had higher growth than those fed the only FO or VO diets (p < 0.05). The highest germinal vesicle migration percentage was observed in FO + VO treatment (p < 0.05). The DHA/EPA, DHA/ARA and EPA/ARA ratios in oocyte exhibited a significant difference in the different treatments (p < 0.05). This study indicates that nutrition of broodstock with diet including FO + VO (p < 0.05) can positively affect the growth performance of larvae compared with only FO or VO diets. Furthermore, the high levels of 18:1n‐9, AL and ALA contents in oocytes from broodstock fed VO and the lowest ALA content in oocytes from broodstock fed FO underlined the important role of broodstock diets in the reproductive process and embryonic and/or larval developments of sterlet. 相似文献
100.