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51.
Pablo Sebastián Reineri María Sumampa Coria Santiago Callejas Gustavo Adolfo Palma 《Anatomia, histologia, embryologia》2020,49(6):820-829
The objectives of the present study were (experiment 1) to characterized development and dynamics of the dominant follicles (DF) and the corpus luteum (CL) to determine patterns of two (W2) and three (W3) follicular waves in beef heifers, and (experiment 2) to determine gene expression of growth factors gene expression in follicular cells of W2 and W3 heifer. Twenty-eight Braford heifers were used. Dominant follicular and CL were monitored daily by ultrasonography to identify the development W2 and W3 in heifers. Pre-ovulatory DF were aspirated on day 19 in W2 and on day 22 in W3 heifers. In W2 and W3, follicular cells (FC) of gene expression of growth differentiation factor 9, bone morphogenetic protein 15 (BMP15), fibroblast growth factor basic, transforming growth factor beta receptor 1, bone morphogenetic protein receptor type IB and fibroblast growth factor receptor 2 were evaluated. The regression of the DF of the first follicular wave and the emergency of the DF of the second follicular wave began later in the heifers W2 than in W3 (p = .02 and p < .01). The regression of the CL began earlier in the W2 than in W3 group (p < .01). Gene expression of growth factors and receptors was similar between groups. However, higher relative levels of BMP15 was observed in W2 group (p = .07). Results propose that wave patterns were regulated by the development time of the DF in the first wave and the life of the CL. Furthermore, higher levels of BMP15 could produce shorter life of CL. The present work suggest that ultrasonography associated with molecular assays could be used as an easy and effective tool to characterize follicular wave patterns. 相似文献
52.
Ana de Santiago Alejandra Expósito José M. Quintero Eusebio Carmona Antonio Delgado 《Journal of plant nutrition》2013,36(2):143-156
Humic substances improve the efficiency of different iron (Fe) sources overcoming Fe deficiency chlorosis of plants. However, applied at high rates, they can promote negative effects on plants. The main objective of this work was to study the potential adverse effect of three humic acids from different origin when they were applied with two effective Fe sources for plants: Fe- ethylenediaminedihydroxyphenylacetic acid (EDDHA) and Vivianite. To this end, an experiment with lupin (Lupinus albus L.) was performed involving two factors: (i) Fe source, and (ii) humic substances from three different origin (composted cork, leonardite, and compost obtained from a mixture of olive husk with cotton gin trash) applied at 0, 0.1, and 0.5 g organic carbon (C) kg?1 of growing media. At the rates used, humic substances promoted adverse effects on plant development, chlorophyll meter readings, and Fe content in lupin grown in calcareous media. Overall, the effect on dry matter and Fe content in plants was more relevant when Fe was supplied with Vivianite, the effect on chlorophyll meter readings being more significant when Fe was applied as Fe-EDDHA. Differences were also observed depending on the source of humic substances, those from leonardite promoting the greatest decrease in dry matter in roots and shoots. These humic substances possessed the highest values of spectroscopy index for aromaticity (A254 ). On the other hand, the application of humic substances from olive husk compost, which exhibited the lower aromaticity index, resulted in the smallest decrease in dry matter production and chlorophyll meter readings. Dry matter in roots decreased logarithmically with increased values of the estimates of the amounts of aromatic compounds accumulated in the growing media (R2 = 0.92; P < 0.01) with Vivianite as Fe source. Thus, the effects decreasing dry matter production, particularly in roots, and chlorophyll meter readings can be ascribed at least partially to the presence of phytotoxic aromatic compounds in humic substances. 相似文献
53.
54.
The loss of connectivity of forest landscapes is seriously hindering dispersal of many forest-dwelling species, which may
be critical for their viability and conservation. In this context, explicitly incorporating connectivity considerations is
an important challenge in current forest planning and management, but as yet there is a lack of operative methods for appropriate
decision making in this respect. We describe a new methodology based on graph structures and a habitat availability index
(integral index of connectivity) that integrates forest attributes (like habitat quality) and network connectivity in a single
measure. We apply this methodology to examine the connectivity of the highly fragmented habitat of capercaillie (Tetrao urogallus) in Catalonia (NE Spain), where the threatened status of this forest bird species calls for landscape-level forest planning
solutions. We analyse data on the distribution of capercaillie forest habitat at 1 km spatial resolution obtained from the
recent Catalan Breeding Bird Atlas. We determine the functionally connected regions existing within its habitat distribution
and identify the forest habitat areas that are more important for the maintenance of overall landscape connectivity for this
species. Based on these results, we provide recommendations on certain critical public forests where management oriented to
the conservation of capercaillie habitat is more necessary. These results highlight the potential and practical interest of
the proposed methodology for successfully integrating landscape connectivity in broad scale forest planning. 相似文献
55.
Rocío Santiago Roberto de Armas Blanca Fontaniella Carlos Vicente María-Estrella Legaz 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(3):439-450
The accumulation of soluble and cell wall-bound phenolics in the sugarcane stems of young plants from highly resistant cv.
My 5514 and susceptible cv. B 42231, inoculated or not inoculated with smut sporidia, was studied. The ratio of inoculated
to uninoculated plants of some cell wall-bound phenolics, such as ferulic, caffeic, and syringic acids increased for the resistant
cv. My 5514, whereas it was maintained more or less constantly for the susceptible cv. B 42231. The highest increase of this
ratio in the resistant cv. My 5514 corresponded to both caffeic and syringic acids. This could result in a better capacity
to cv. My 5514 for an increase in the frequency of bridges between lignin fragments through ester-ether linkages for reinforcing
the cell wall and major resistance to the disease. This reinforcement of the cell wall could provide an effective barrier
to pathogen entry and spread. Soluble sub-fractions of all phenolics detected showed non-stable patterns. Caffeic acid, that
regulates phenylalanine ammonia-lyase activity in sugarcane, showed a significant decrease in its titre at 24 h in the resistant
cultivar, principally in the free soluble fraction, whilst the susceptible cultivar enhanced it. We hypothesise that the pathway
of hydroxybenzoic acids is only activated once the level of p-coumaric acid justifies the accumulation of hydroxycinnamic acids required for reinforcing the cell wall after inoculation. 相似文献
56.
J Santiago Moreno A Gomez Brunet A Gonzalezde Bulnes D Villar A Lopez Sebastian 《Reproduction in domestic animals》2000,35(2):49-52
The onset of puberty was assessed from plasma progesterone profiles for Mouflon (n = 8) and Manchega (n = 7) female lambs born in April. Four Mouflons exhibited their first ovulation at a mean date of 27 November ± 1.4 days, the mean age being 248.5 ± 3.9 days and the mean body weight 23.8 ± 0.6 kg. The remaining four Mouflons, which had a slower growth rate, attained puberty during the autumn of the following year. Five of seven Manchega lambs attained puberty in their first year at a mean date of 5 Oct ± 3.3, the mean age being 185.6 ± 2.6 days and the mean body weight 41.8 ± 2.0 kg. The mean live‐weight at the onset of puberty was 65.1 and 82% (p < 0.05) of adult body weights for Manchega and Mouflons, respectively. Overall, the results indicate that there is a threshold of body‐weight necessary for the attainment of puberty in the first breeding season for spring‐born lambs, which is very different between both Mouflon and Manchega. When body weight was below 23.8 ±0.6 and 41.8 ± 2.0 kg for Mouflon and Manchega lambs, respectively, first ovulation did not occur until the beginning of the next breeding season. The onset of puberty during the second year of life occurred within the specific reproductive seasons for Mouflon (October–April) and Manchega (July–March) ewes, despite minimal further growth. 相似文献
57.
Hematologic and biochemical reference intervals for Wild Boar (Sus scrofa) captured by cage trap
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58.
Costa Evely Giovanna Leite da Silva Fabiano Ferreira Silva Robério Rodrigues Porto Antonio Ferraz Santiago Bismarck Moreira Rocha Leone Campos Cruz Aline Gonçalves Guedes Anna Caroline Ferreira Neto Teotônio Martins Vieira Edvaldo Alves 《Tropical animal health and production》2019,51(8):2505-2511
Tropical Animal Health and Production - The objective of this study was to evaluate the inclusion of increasing levels of licuri meal as a substitute for soybean bran in the diet of pasture-based... 相似文献
59.
Influence of Post‐Mortem Sperm Recovery Method and Extender on Unstored and Refrigerated Rooster Sperm Variables
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S Villaverde‐Morcillo MC Esteso C Castaño J Santiago‐Moreno 《Reproduction in domestic animals》2016,51(1):40-46
Many post‐mortem sperm collection techniques have been described for mammalian species, but their use in birds is scarce. This paper compares the efficacy of two post‐mortem sperm retrieval techniques ‐ the flushing and float‐out methods ‐ in the collection of rooster sperm, in conjunction with the use of two extenders, i.e., L&R‐84 medium and Lake 7.1 medium. To determine whether the protective effects of these extenders against refrigeration are different for post‐mortem and ejaculated sperm, pooled ejaculated samples (procured via the massage technique) were also diluted in the above extenders. Post‐mortem and ejaculated sperm variables were assessed immediately at room temperature (0 h), and after refrigeration at 5°C for 24 and 48 h. The flushing method retrieved more sperm than the float‐out method (596.5 ± 75.4 million sperm vs 341.0 ± 87.6 million sperm; p < 0.05); indeed, the number retrieved by the former method was similar to that obtained by massage‐induced ejaculation (630.3 ± 78.2 million sperm). For sperm collected by all methods, the L&R‐84 medium provided an advantage in terms of sperm motility variables at 0 h. In the refrigerated sperm samples, however, the Lake 7.1 medium was associated with higher percentages of viable sperm, and had a greater protective effect (p < 0.05) with respect to most motility variables. In conclusion, the flushing method is recommended for collecting sperm from dead birds. If this sperm needs to be refrigerated at 5°C until analysis, Lake 7.1 medium is recommended as an extender. 相似文献