Flood or drought: how do aerosols affect precipitation?

Aerosols serve as cloud condensation nuclei (CCN) and thus have a substantial effect on cloud properties and the initiation of precipitation. Large concentrations of human-made aerosols have been reported to both decrease and increase rainfall as a result of their radiative and CCN activities. At one extreme, pristine tropical clouds with low CCN concentrations rain out too quickly to mature into long-lived clouds. On the other hand, heavily polluted clouds evaporate much of their water before precipitation can occur, if they can form at all given the reduced surface heating resulting from the aerosol haze layer. We propose a conceptual model that explains this apparent dichotomy.     

Multiclass method for the determination of pesticides in stem of coconut palm using matrix solid-phase dispersion prior to liquid chromatography analysis

A method was developed using matrix solid-phase dispersion and liquid chromatography with ultraviolet diode array detection for the determination of bromopropylate, clofentezine, coumaphos, difenoxuron, diniconazole, fenvalerate, flumetralin, fluometuron, and teflubenzuron in the stem of coconut palm. The parameters evaluated included the type of sorbent (C₁₈-bonded silica, neutral alumina, silica gel, and Florisil) and the volume of the eluting solvent, acetonitrile. The best results were obtained using 0.25 g of stem, 1.0 g of neutral alumina as sorbent, and 5 mL of acetonitrile. The method was validated using stem samples spiked with pesticides at different concentration levels (0.01–10.0 µg mL^?1). Average recoveries (using three replicates) ranged from 70 to 91 %, with relative standard deviations between 1.6 and 10 %. Detection and quantification limits were in the ranges 0.01–0.05 and 0.05–0.2 µg g^?1, respectively.     

Characterization of the volatile constituents in the essential oil of Pistacia lentiscus L. from different origins and its antifungal and antioxidant activity

Essential oil (EO) from aerial parts (leaves, juvenile branches, and flowers when present) of Pistacia lentiscus L. growing wild in five localities of Sardinia (Italy) was extracted by steam-distillation (SD) and analyzed by gas chromatography (GC), FID, and GC-ion trap mass spectrometry (ITMS). Samples of P. lentiscus L. were harvested between April and October to study the seasonal chemical variability of the EO. A total of 45 compounds accounting for 97.5-98.4% of the total EO were identified, and the major compounds were alpha-pinene (14.8-22.6%), beta-myrcene (1-19.4%), p-cymene (1.6-16.2%), and terpinen-4-ol (14.2-28.3%). The yields of EO (v/dry w) ranged between 0.09 and 0.32%. Similar content of the major compounds was found in samples from different origins and seasonal variability was also observed. The EOs were tested for their antifungal activity against Aspergillus flavus, Rhizoctonia solani, Penicillium commune, Fusarium oxysporum. Two samples were weakly effective against Aspergillus flavus. Furthermore, terpinenol and alpha-terpineol, two of the major components of EO of Pistacia lentiscus L., totally inhibited the mycelian growth of A. flavus. Quite good antioxidant activity of the EO was also found.     

Molecular detection of norovirus in sheep and pigs in New Zealand farms

Human norovirus (NoV) is reportedly the major cause of non-bacterial gastroenteritis outbreaks worldwide and is commonly associated with water- and food-borne transmission via the faecal-oral route. Aside from humans, norovirus has been detected in pigs, cattle and mice. The close relatedness of some human and animal noroviruses has raised concerns about potential zoonotic transmission. Our laboratory recently reported the development of a multiplex real-time RT-PCR for the detection and genotyping of norovirus of genogroups I-III. Here we report a study of 56 faecal specimens from pigs and sheep that were collected and screened for noroviruses using this assay. Norovirus was found in 2/23 (9%) of porcine specimens (all were genogroup II) and in 8/33 (24%) of ovine specimens (all were genogroup III). Samples tested positive for norovirus were verified by conventional RT-PCR with different primer sets. Genomes of representative porcine and ovine norovirus strains underwent partial sequence analysis (343 and 2045 bases, respectively). This is the first report describing norovirus in sheep.     

The path from β-carotene to carlactone, a strigolactone-like plant hormone

Strigolactones, phytohormones with diverse signaling activities, have a common structure consisting of two lactones connected by an enol-ether bridge. Strigolactones derive from carotenoids via a pathway involving the carotenoid cleavage dioxygenases 7 and 8 (CCD7 and CCD8) and the iron-binding protein D27. We show that D27 is a β-carotene isomerase that converts all-trans-β-carotene into 9-cis-β-carotene, which is cleaved by CCD7 into a 9-cis-configured aldehyde. CCD8 incorporates three oxygens into 9-cis-β-apo-10'-carotenal and performs molecular rearrangement, linking carotenoids with strigolactones and producing carlactone, a compound with strigolactone-like biological activities. Knowledge of the structure of carlactone will be crucial for understanding the biology of strigolactones and may have applications in combating parasitic weeds.     

Site quality evaluation by classification tree: an application to cork quality in Sardinia

Cork harvesting and stopper production represent a major forest industry in Sardinia (Italy). The target of the present investigation was to evaluate the classification tree as a tool to discover possible relationships between microsite characteristics and cork quality. Seven main cork oak (Quercus suber) producing areas have been identified in Sardinia, for a total of more than 122,000 ha. Sixty-three sample trees, distributed among different geographical locations and microsite conditions, were selected. A soil profile near each sample tree was described, soil samples were collected and analysed. After debarking, cork quality of each sample tree was graded by an independent panel of experts. Microsites where trees had more than 50% of the extracted cork graded in the best quality class, according to the official quality standard in Italy, were labelled as prime microsites, the others as nonprime microsites. Relationships between a binary dummy variable (0 for nonprime microsites, 1 for prime microsites) and site factors were investigated using classification tree analysis to select the relevant variables and to define the classification scheme. Prime quality microsites for cork production proved to be characterised by elevation, soil phosphorus content and sandiness. Results have been compared with those of the more conventional parametric approach by logistic regression. The work demonstrates the advantages of the classification tree method. The model may be appropriate for classifications at landscape and stand mapping levels, where it is possible to sample a number of microsites and to evaluate distributional characteristics of model output, while its precision is only indicative when estimating the prime quality of single microsites.     

Evaluation of bulk density of Albaqualf soil under different tillage systems using the volumetric ring and computerized tomography methods

The volumetric ring and the computerized tomography (CT) techniques were applied to study soil bulk density, in order to understand the compaction of an Albaqualf soil (Planosol) of the Rio Grande do Sul State, Southern Brazil (latitude 31°52′00″S and Longitude 52°21′24″W). Among six different tillage systems and crop rotations the greatest soil bulk density was measured for the continuous irrigated rice crop system and the lowest for the no-tillage treatment under rye grass straw. The CT method enabled the measurement of bulk density variations in the soil profile and indicated critical zones not observed by the volumetric ring method that measures only the mean sample soil bulk densities. A meaningful correlation between soil bulk densities measured by both methods was found, although the CT method presented more reliable results in comparison to the volumetric ring method. A 3% variation in bulk density was observed due to method intrinsic errors, probably also correlated to different samples sizes.     

Benzodioxole derivatives as negative effectors of plant proteases

Previous work demonstrated that a commercial formulation of piperonyl butoxide (PBO) did inhibit the activity of some plant proteolytic enzymes. In this paper, the effect of pure PBO and nine pure PBO homologues (PBOH) appropriately synthesized toward bromelain and papain was studied in hydrocarbon solution using the bis(2-ethylhexyl)sodium sulfosuccinate (AOT) reverse micellar system. This study establishes that the majority of these compounds show, in vitro, interesting protease inhibition activities. The benzodioxole and dihydrobenzofuran structures, in particular, 5-[2-(2-butoxyethoxy)ethoxymethyl]-benzo[1,3]dioxole (EN 1-40) and 6-[2-(2-butoxyethoxy)ethoxymethyl]-5-propyl-2,3-dihydrobenzofuran (EN 16-5), respectively, appear to be responsible for protease inhibition. Measures of octanol/water partition coefficients on PBO and PBOH have demonstrated that water solubility plays a fundamental role in the expression of protease inhibition activity.     

An Automated Fog Water Collector Suitable for Deposition Networks: Design,Operation and Field Tests

The study of fog water chemical composition and the contribution of fog droplets to total chemical deposition has become a relevant environmental subject over the past few years. This paper describes a fog water collector suitable for deposition network operation, due to its complete automation and to the facility of remote acquisition of sampling information. Sampling of fog droplets on teflon strings is activated by an optical fog detector according to a particular protocol operated by a microprocessor controller. Multiple sample collection, also microprocessor controlled, is possible with this instrument. The problem of fog droplet sampling in sub-freezing conditions is overcome using a sampling schedule implemented by the microprocessor controller which alternates between sampling periods and stand-by periods during which melting of the rime collected on the strings is allowed. Field tests on the reliability and reproducibility of the sampling operations are presented in the paper. Side by side operation of the fog collector with a PVM-100 fog liquid water content meter shows that the amount of water per unit volume of air collected by the sampling instrument is proportional to the fog liquid water content averaged over the period of an entire fog event.     

Water Remediation by Adsorption of Phenol onto Hydrophobic Modified Clay

In this work we studied the phenol sorption from contaminated water onto modified clay mineral vermiculite and bentonite by insertion of hexadecyltrimethylammonium in the interlayer of clays. The non modified clays showed negligible interaction with phenol. The experimental data were treated using the Freunlich equation which had a good fit. The removal percentages were higher than 35% for modified vermiculite and for modified bentonite, 30%. Vermiculite and bentonite were compared for the removal of phenol from an aqueous solution based on the maximum adsorption capacity of each adsorbent, and no significant difference was found. The adsorption mechanism for both is the same, according to the results. Although, there is a lack of studies using vermiculite for this purpose, it can be concluded that hydrophobic modified vermiculite is very effective for removing phenol from water. Hydrophobic modified bentonite also remove phenol, but in lower proportions.