小麦两个杂交组合后代PPO活性的遗传分析

用全麦粉分别以多巴和邻苯二酚为底物测定了小麦组合(烟农19×安农0016 和烟农19×安农98017)早代(F3)株系的PPO活性变异.结果表明,多巴和邻苯二酚法检测结果具有可比性(烟农19×安农0016)F3代的117个株系相关系数高达0.907.因此,这一检测方法适于进行PPO活性的遗传分析研究.两个杂交组合的PPO活性受两对基因控制,并具有较高的遗传率.在组合烟农19×安农0016中,第一对主基因的加性效应较明显,远大于第二对主基因,而在组合烟农19×安农98017中,两对主基因的加性效应则大小一致.     

水肥一体化椰糠栽培技术对日光温室蔬菜品质的影响

测定了自主研发的水肥一体化椰糠栽培技术对日光温室中7种蔬菜作物品质的影响。结果表明:该技术可以提高受试蔬菜可溶性糖、维生素C、可溶性固形物和矿质营养元素的含量,但对可溶性蛋白质、有机酸、硝酸盐含量等其他指标的影响表现出了品种差异。总体上来看,水肥一体化椰糠栽培技术提高了多个受试蔬菜品种的营养品质。     

氮肥运筹对垄作小麦生育后期光合特性及产量的影响

为给垄作小麦生产提供理论依据与技术支持,采用正交设计的试验方法,研究了氮素运筹对垄作小麦生育后期旗叶光合特性及产量的影响.结果表明,适当增加施氮量,能够显著提高垄作小麦生育后期旗叶的光合速率及产量.在施氮量(纯氮)为每公顷250 kg左右时,小麦旗叶光合速率最高,产量也最高.在氮肥用量一定的情况下,33%播种前基施,67%拔节期追施,小麦后期光合速率和产量最高.垄作小麦在开花期光合速率略低于平作小麦,但自灌浆开始旗叶光合速率一直高于传统平作小麦,且差异显著.虽然垄作小麦的公顷穗数显著小于平作小麦,但是前者的穗粒数和千粒重却都显著大于后者,因而垄作小麦的产量也显著高于平作小麦.在较高肥力条件下,小麦采用垄作栽培方式,氮肥总用量250 kg/ha左右,基追比为1∶2时,可以获得较高的籽粒产量.     

绿头鸭线粒体DNA控制区全序列测定及分析

用PCR产物直接测序法,获得5只绿头鸭线粒体DNA控制区(D-loop)全序列.序列分析显示:绿头鸭D-loop区全长为1051bp,碱基A、G、T、C含量分别为27.97%、15.51%、25.21%、31.30%,A+T含量大于C+G;共检测到11个多态位点,其中转换10个,颠换1个,没有发现插入和缺失,核苷酸多样度为0.0046.结合GenBank已公布河鸭属鸭类D-loop区序列,基于邻接法和最小进化法构建河鸭属鸭类系统进化树.结果表明,绿头鸭、斑嘴鸭及家鸭三者关系密切,它们共同构成进化枝A,推测在家鸭的形成过程中,绿头鸭和斑嘴鸭都作出了贡献;其它河鸭类聚为进化枝B.     

Prevention of isinglass on the chronic atrophic gastritis in rats and its mechanism

AIM: To evaluate the effect of isinglass on chronic atrophic gastritis(CAG) in rats and its mechanism. METHODS: An animal model of CAG in accordance with the previous experience of combined administration of 60% ethanol, 20 mmol/L sodium deoxycholate and 0.1% ammonia water was established in SD rats. Isinglass was used as preventive therapy while we were establishing CAG rat model. Finally all the rats were executed and pathologic changes of the gastric mucosa were studied by gross appearance and microscopy and serum epidermal growth factor (EFG) and growth hormone(GH) contents were tested. RESULTS: In each isinglass prevention group, inflammation grade of gastric antrum was less than that in model group (P<0.01) while the mean ratio of the thickness of gastric mucosal gland and muscularis mucosa (L1/L2), the number of gastric glands in 1 mm lengths of mucosal layer in longitudinal sections were much better than those in model group (P<0.01).They were very close to normal control group (P>0.05). The expression of proliferating cell nuclear antigen (PCNA) in gastric mucosa and serum EFG level were higher than those in model group (P<0.01, P<0.05), but serum GH content showed no different between isinglass prevention group and model group. CONCLUSION: Isinglass preventes the gastric mucosal atrophy in the CAG model. Its mechanism may be related to the effects of decreasing the gastric mucosal damage， promoting the cell proliferation and increasing of internal EFG secretion.     

Effect of rhEGF activating extracellular signal-regulated kinase on the expression of Bcl-2 and p53 protein in human amniotic cells

AIM: To explore the effect of recombinant human epidermal growth factor (rhEGF) on the ERK1/2 pathway and its safety in use when the proliferation of cultured FL cells (human amnion epithelial cells) were promoted by rhEGF.METHODS: FL cells were stimulated by rhEGF at various concentrations.The proliferation rate of FL cells was evaluated by MTT assay.Western blotting was used to detect phosphorylation of ERK1/2 (p-ERK1/2) and the change of Bcl-2 and P53 protein.RESULTS: The proliferation rate FL cells reached the maximum when the concentration of rhEGF was 10 μg/L (42.4％，P<0.01).Western blotting showed that the activity of p-ERK1/2 increased significantly when rhEGF ranged in 10 μg/L to 60 μg/L.Level of Bcl-2 was unchanged in each groups.Level of P53 decreased significantly when the concentration of rhEGF was 60 μg/L.CONCLUSION: It is safe when rhEGF presents in optimization concentration of 10 μg/L.The capability of apoptosis may be depressed when rhEGF is in larger dose.     

废弃矿山挂网喷播土壤理化性质动态变化

对徐州市王山废弃矿山挂网喷播前后土壤理化性质动态变化进行了分析.结果表明:挂网喷播后,土壤水土流失量降低,抗蚀性明显提高,土壤总孔隙度和田间持水量增加,土壤密度和pH值降低,土壤养分含量及综合肥力指数明显提高.     

4味中药及其与抗菌药的复方制剂的MIC测定

用改进的试管两倍稀释法测定了黄连、鱼腥草、大青叶和苦参4味中药及其相互配伍（1：1）对标准大肠杆菌的最小抑菌浓度和4味中药与抗菌药配伍（1000：1）对临床分离鸡大肠杆菌的最小抑菌浓度。结果表明，4味中药及其相互配伍对大肠杆菌标准株有一定的抑制作用，其MIC值介于3．91—62．50mg／mL之间，其中大青叶和鱼腥草以1：l配伍对大肠杆菌抑菌效果最好，MIC为3．91mg／mL；对临床分离株，单方药中黄连抑菌效果最好，MIC为31．25mg／mL，24种复方药中，黄连和盐酸多西环素或加替沙星以1000：1配伍的抑菌效果最好，其MIC为3．91mg／mL。     

施用生物菌剂对烤烟叶片生理特征及钾、氯含量的影响

为探讨不同生物菌剂在烤烟上的施用效果,选取烤烟品种秦烟96和豫烟6号为材料,设计了对照(T1)、生物菌剂A(T2)、生物菌剂B(T3)、生物菌剂C(T4)、生物菌剂D(T5)和生物菌剂E(T6)6个处理,于2016年在河南省洛阳市(旱作烟区)和驻马店市(雨养烟区)进行了田间试验,研究了施用生物菌剂对烤烟叶片生理特征及钾、氯含量的影响。结果表明:施用生物菌剂处理与对照相比烤烟叶片中可溶性糖的含量增加了0.38%～1.43%,丙二醛(MDA)含量降低了0.3～7.36μmol·g^-1。除雨养烟区T5处理外,与对照相比,施用生物菌剂处理烤烟叶片过氧化氢酶(CAT)活性、超氧化物岐化酶(SOD)活性和过氧化物酶(POD)活性分别提高2.1～26.2μmol·min^-1·g^-1,0.6～40 U·g^-1和546～2 531 U·g^-1。中部叶养分分析表明,施用生物菌剂处理在移栽后45 d(除雨养烟区T4处理外)全钾含量提高了0.42%～1.32%,移栽后60 d提高了0.03%～1.00...     

(土娄)土区不同蔬菜对钾肥的肥效反应

在陕西省关中土娄土区布置线辣椒、甘蓝、白菜、芹菜钾肥大田试验,研究不同蔬菜对钾肥的肥效反应。结果表明,增施钾肥可使蔬菜硝态氮含量降低11.8%～116.0%,维生素C含量增加0.4～147.4mg/kg;产量增加9.3%～33.8%;明显促进营养生长和生殖生长;400cm深土壤剖面硝态氮累积量减少22.7～48.0kg/hm2,尤其在80～220cm剖面减少最为明显。