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Mechanical transmission of porcine reproductive and respiratory syndrome virus (PRRSV) throughout a coordinated sequence of events that replicated common farm worker behavior during warm weather (10°C to 16°C) was assessed using a field-based model. The model involved fomites (boots and containers), vehicle sanitation, transport, and personnel movement. In a previous study, the model successfully demonstrated mechanical transmission of PRRSV in 8 out of 10 replicates during cold weather. A field strain of PRRSV was inoculated into carriers consisting of soil samples, which were adhered to the undercarriage of a vehicle. The vehicle was driven approximately 50 km to a commercial truck washing facility where the driver's boots contacted the carriers during washing, introducing the virus to the vehicle interior. The vehicle was then driven 50 km to a simulated farm site, and the driver's boots mechanically spread virus into the farm anteroom. Types of containers frequently employed in swine farms contacted drippings from the footwear on the anteroom floor. The truck wash floor, vehicle cab floor mats, boot soles, anteroom floor, and the ventral surface of containers were sampled to track the virus throughout the model. Ten replicates were conducted, along with sham-inoculated controls, and control replicates. In 2 replicates, infectious PRRSV was detected on the anteroom floor and in 1 replicate, infectious PRRSV was detected on the surface of the container by swine bioassay. All sham-inoculated controls and protocol controls were negative. These results indicate that mechanical transmission of PRRSV throughout a coordinated sequence of events in warm weather can occur, but in contrast to data from studies conducted during cold weather, it appears to be a relatively infrequent event.  相似文献   
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Three inventories were conducted to quantify Bangkok's green infrastructure for future planning and improvement in the context of a seasonal monsoonal dry climate. Total green space was quantified by extracting surface cover areas from remotely sensed data in a geographical information system (GIS) environment, and this information was used to designate suitable sites for future green spaces such as parks. Street trees were inventoried for species identification and size. Trees of heritage value were identified through a public awareness campaign, and then were subsequently surveyed for species identification, height and trunk diameter. GIS green space analysis showed that per capita park space was approximately 1.8 m2, but a master plan proposed increasing per capita park area to 4 m2 within 25 years. The increased park area will be largely in the form of lower cost, semi-naturalized tree parks. The inventoried street tree population, approximately 200,000, was skewed somewhat towards a monoculture, as 42% were the facultative evergreen Pterocarpus indicus Wild. By contrast, none of the other species exceeded 7% of the total. That most of the other species consisted of smaller trunk diameters than P. indicus, and therefore younger, suggests that Bangkok's street tree plantings are becoming more diverse. The heritage large tree inventory was dominated by evergreen tree species, particularly exceptionally large Ficus species, found largely on Buddhist temple grounds, followed by Albizia saman (Jacq.) Merr. The slower growing evergreen heritage species are worth careful appraisal and preservation because they are less likely to be commonly planted. Careful species selection balancing drought deciduous and dry evergreen species can achieve adaptation to the monsoonal dry season with diverse aesthetic quality in both Bangkok's street tree population and in its semi-naturalized tree parks.  相似文献   
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Chronic tributyltin toxicity experiments were conducted with the following Chesapeake Bay organisms: amphipod, Gammarus sp.; juvenile Atlantic menhaden, Brevoortia tyrannus and larval inland silverside, Menidia beryllina. TBT concentrations ranging from 29 to 579 ng L?1 did not significantly affect survival of the benthic amphipod, Gammarus sp. after 24-d exposures. The weight of Gammarus exposed to control conditions was 2.8 times greater than the weight of these test organisms exposed to 579 ng L?1 TBT. Twenty-eight day exposures to TBT concentrations of 93 and 490 ng L?1 did not significantly affect survival of juvenile B. tyrannus or larval M. beryllina. Histological examinations of B. tyrannus did not demonstrate absolute effects resulting from TBT exposure due to extensive variation between individuals. Various morphometric measurements of M. beryllina after TBT exposure did not demonstrate significant effects. However, significant reductions in growth were reported for M. beryllina at both TBT concentrations. Environmental concentrations of TBT in Chesapeake Bay and possible effects on the above biota are discussed.  相似文献   
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Given the importance placed on protected areas, determining their effectiveness in representing and maintaining biodiversity is a core issue in conservation biology. Nonetheless, frameworks identifying the breadth of issues associated with this effectiveness, and case studies of how well these are understood in particular regions, remain lacking. In this paper, we provide such a framework and an overview of the current state of knowledge of the ecological effectiveness of protected areas in the United Kingdom. Arguably, better data are available to address such issues in this region than anywhere else in the world. Nonetheless, studies remain scarce and have focussed foremost on the, rather narrow, issue of the effectiveness of management actions on individual sites in order to deliver fixed conservation objectives and discharge statutory responsibilities. Some attention has also been paid to how well the regional collection or portfolio of protected areas performs, particularly in capturing biodiversity features. Work on the extent to which protected areas in the United Kingdom form effective functional networks is in its infancy, but initiatives are under development. We identify some of the questions about the effectiveness of protected areas to which answers need to be known at the site, portfolio and network levels, and how significant progress might be achieved in addressing these.  相似文献   
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中国大多数桉树资源主要集中在较为温暖的沿海地区.目前在湖南和江西等华南和中南省份较为冷凉的内陆地区,通过选择一些合适的耐寒桉树品种,种植了一些规模不大的桉树人工示范林,这些示范林显示出了较好的适应性与丰产性.这些较为冷凉的内陆地区有着立地条件较为优越的适合人工林发展的广大林地.对于较冷地区,尤其是湖南省来说,通过分析所...  相似文献   
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