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991.
Piérri Spolti Luana Schneider Rosa M. V. Sanhueza Jean C. Batzer Mark L. Gleason Emerson Medeiros Del Ponte 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(1):21-29
Sooty blotch and flyspeck is caused by numerous species of fungi that colonize the surface of apple fruit and thereby lower
its market value. Although this disease poses a substantial threat to apple growers’ profitability in some regions, reliable
and cost-effective methods for epidemiological and disease control studies have not been validated, nor are they widely available.
We modified a standard area diagram to aid sooty blotch and flyspeck severity assessments and quantified its impact on accuracy
and precision of visual estimates. Samples of ‘Fuji’ and ‘Mutsu’ fruit were photographed both from the top and laterally.
Severity was assessed from a sub-sample of 160 images using image analysis software. Validation of the diagram was performed
by eight raters who independently assessed severity in two series of selected images representing the lateral view and the
top view, initially unaided and subsequently with the aid of the scale. Severity estimates ranged from 0.4% to 98% (most fruit
had <10% severity). Accuracy and precision of the estimates were significantly improved when using the diagrammatic scale;
concordance correlation coefficient values increased from 0.81 to 0.95. A strong tendency to underestimate severity for the
mid-range to high levels was minimized when using the aid, which also improved reproducibility of the estimates among raters.
In addition to strengthening evidence that a standard area diagram can be used reliably in sooty blotch and flyspeck studies,
we expanded its application to disease assessment in the peduncle region, which enhances the usefulness of the method for
evaluating efficacy of management practices. 相似文献
992.
Warrick R. Nelson Tonja W. Fisher Joseph E. Munyaneza 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(1):5-12
Three haplotypes of the recently discovered bacterium species “Candidatus Liberibacter solanacearum” are described and related to geographic ranges. The first two are associated with Zebra Chip/Psyllid
Yellows of potatoes and other solanaceous plants, vectored by the tomato/potato psyllid Bactericera cockerelli in North and Central America and New Zealand. The third is associated with diseased carrots in Finland and vectored by the
carrot psyllid Trioza apicalis. The haplotypes are described by SNPs on the 16s rRNA, 16s/23s ISR and 50s rplJ and rplL ribosomal protein genes. These SNPs
are inherited as a package across the three genes. Haplotype “a” has been found primarily from Honduras and Guatemala through
western Mexico to Arizona and California, and in New Zealand. Haplotype “b” is currently known from eastern Mexico and northwards
through Texas to south central Washington. These haplotypes show some range overlap in Texas, Kansas and Nebraska. The haplotypes
are not yet known to elicit biological differences in the plant or insect hosts. These apparently stable haplotypes suggest
separate bacterial populations of long standing. 相似文献
993.
Odile Carisse Catherine Meloche William W. Turechek 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(3):349-365
The spatial pattern of apple scab was characterized using 10 years of disease incidence and lesion density data collected
in managed orchards located in Quebec, Canada. Distributional analyses indicated that scab incidence was better characterized
by the beta-binomial than the binomial distribution in 53 and 65% of the data sets at the leaf and shoot scales, respectively.
Median values of the beta-binomial parameter θ, a measure of small-scale aggregation, were near 0 (0.003 and 0.028) at both
sampling scales, indicating that disease incidence was close to being randomly distributed (low degree of aggregation). For
lesion density, the negative binomial distribution fitted the data better than the Poisson distribution in 86% of the data
sets at the leaf scale. The median value of the index of dispersion k was 0.068, indicating that aggregation was present. For all apple scab measurements, the power law models provided a good
fit to the data. The estimated slope and intercept parameters were significantly greater than 1 and 0, respectively, suggesting
that spatial heterogeneity changed systematically with disease incidence. Results of a covariance analysis showed that spatial
heterogeneity of scab incidence at both scales and lesion density was not dependent upon shoot type but that spatial heterogeneity
of scab incidence and lesion density at the leaf scale was influenced by the sampling period. A hierarchical analysis showed
that scab incidence at the tree scale increased as a saturation-type curve with respect to incidence at the leaf or shoot
scales. A similar relationship was observed for incidences at the shoot and leaf scales. An effective sample size model based
on the binary power law parameters (Madden and Hughes, Phytopathology 89:770–781, 1999) gave the best fit to the leaf and shoot data, respectively. The incidence-lesion density relationship at both scales was
well described by a complementary log-log (CLL) and log transformation model ( Radj2 = 0.97 and Radj2 = 0.94 ) \left( {R_{{adj}}^2 = 0.97\,and\,R_{{adj}}^2 = 0.94} \right) , however, the models tended to underestimate lesion density. The information of the spatial relations of apple scab within
and between hierarchical scales acquired from this study can be used in developing and evaluating practical disease management
strategies and to improve apple scab assessments for fungicide or cultivar susceptibility trials. 相似文献
994.
Otmar Spring Thines Marco Stefanie Wolf Reinhard Zipper 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(3):519-527
Sequencing of partial cox2 (part of the mitochondrial cytochrome-c-oxydase (COX) gene) was performed with samples from the oomycete genus Pustula, the white blister rusts of Asteraceae and related families. Sequence comparison uncovered several single nucleotide polymorphisms
(SNPs) between P. spinulosa and host specific strains of Pustula isolated from Senecio vulgaris, Tragopogon pratensis and cultivated sunflower, Helianthus annuus. Based on these differences, specific primers were designed for PCR-based detection of white blister rust strains pathogenic
to sunflower. The specificity of the primers was confirmed by cross testing with DNA from various oomycetes occurring in the
same locality. The limit of detection for DNA of P. helianthicola was 10 pg. This allowed detection with DNA from single sporangia and single oospores. The PCR-based experiments allowed detection
of the presence of sunflower white blister rust in soil samples from fields on which infected plants had been cultivated several
months before. Moreover, the molecular tools were successfully applied to trace the pathogen in asymptomatic tissue of infected
plants, demonstrating the systemic nature of Pustula on sunflower. 相似文献
995.
Monique L. Sakalidis Jane D. Ray Vincent Lanoiselet Giles E. StJ. Hardy Treena I. Burgess 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(3):379-391
Members of the Botryosphaeriaceae, in particular Lasiodiplodia theobromae, Neofusicoccum parvum, N. mangiferum and Botryosphaeria dothidea, commonly cause stem cankers, dieback and stem end rot of mangoes worldwide. In the current study, eight taxa of Botryosphaeriaceae
were identified as canker-associated fungi, pathogens, potential pathogens or endophytes of mangoes in the Kimberley, Australia.
These include Neoscytalidium novaehollandiae, Ne. dimidiatum, Pseudofusicoccum adansoniae, P. ardesiacum, P. kimberleyense, Lasiodiplodia sp. 1, L. iraniensis and L. pseudotheobromae. The pathogenicity of a selection of these species toward fruit and branches was tested. All were pathogenic to mango in comparison
to the control, with Lasiodiplodia spp. being the most pathogenic. It appears that either geographic isolation or the unique growing conditions in the Kimberley
may have provided an effective barrier to the acquisition or establishment of known botryosphaeriaceous pathogens. Wounds
caused by mechanical pruning may provide an entry point for infection, whilst severe pruning may increase plant stress. 相似文献
996.
Ferenc Viranyi Otmar Spring 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(2):207-220
This review summarises the progress in research on sunflower downy mildew as reported in publications of the past 10 to 15 years,
the period since the last comprehensive review on Plasmopara halstedii. Particular attention is paid to subjects that showed much progress and may be of particular interest to sunflower pathologists,
mycologists or molecular biologists. Accordingly, single sections are devoted to the problems of taxonomic and phylogenetic
aspects, host specificity, the host—pathogen interaction including resistance phenomena, as well as epidemiology and disease
management. Reflecting the progress achieved in some fields and illuminating the deficits in others should stimulate the reader's
interest in this very significant pathosystem. 相似文献
997.
Marie-France Corio-Costet Marie-Cécile Dufour Jérémy Cigna Pierre Abadie Wei-Jen Chen 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(2):315-329
The effectiveness of Quinone outside Inhibitor (QoI) fungicides against grape downy mildew in European vineyards has significantly
decreased in the last decade. One nucleotide polymorphism, G143A in the cytochrome b gene of Plasmopara viticola, is involved in resistance to QoIs. Previous genetic examination on the mitochondrial genomes showed four major haplotypes
(IR, IS, IIR, IIS) coexisting in European vineyards. A resistant allele (G143A) was present in IR and IIR haplotypes. The
purpose of the present study was to estimate the diversity of the different mitochondrial haplotypes and their distribution
in QoI-resistant populations before evaluating the potential cost of the resistant mutation G143A in P. viticola population. From 2000 to 2004, the frequencies of resistant isolates ranged from 0% to 23.25% with an average of 4.64 % among
the populations examined. To evaluate the fitness of sensitive and resistant isolates, a comparison of different biological
parameters including latent period, spore production and infection frequency was performed, enabling a fitness index (FI) to be determined. Resistant isolates exhibited greater infection frequency than sensitive isolates, whereas no significant
difference was found in sporulation ability and latent period between sensitive and resistant isolates. To further investigate
competitiveness among isolates, an assay including two resistant isolates in different proportion with a sensitive isolate
was conducted on eight asexual growing cycles in the absence of a QoI fungicide. The competitiveness of resistant isolates
varied according to their fitness parameters, suggesting that there is no noticeable cost of QoI resistance in controlled
conditions in Plasmopara viticola. 相似文献
998.
Marie Gosme Philippe Lucas 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(1):119-131
The importance of the spatial aspect of epidemics has been recognized from the outset of plant disease epidemiology. The objective
of this study was to determine if the host spatial structure influenced the spatio-temporal development of take-all disease
of wheat, depending on the inoculum spatial structure. Three sowing patterns of wheat (broadcast sowing, line sowing and sowing
in hills) and three patterns of inoculum (uniform, aggregated and natural infestation) were tested in a field experiment,
repeated over 2 years. Disease (severity, root disease incidence, plant disease incidence and, when applicable, line and hill
incidences) was assessed seven times during the course of each season and the spatial pattern was characterized with incidence-incidence
relationships. In the naturally infested plots, disease levels at all measurement scales were significantly higher in plots
sown in hills, compared to plots sown in line, which were in turn significantly more diseased than plots with broadcast sowing.
Disease aggregation within roots and plants was stronger in line and hill sowing than in broadcast sowing. Analysis of the
disease gradient in the artificially infested plots showed that the disease intensified (local increase of disease level)
more than it extensified (spatial spread of the disease), the effect of the introduced inoculum was reduced by 95% at a distance
of 15 cm away from the point of infestation. Yield was not significantly affected by sowing pattern or artificial infestation. 相似文献
999.
Oliver A. H. Jones Mahon L. Maguire Julian L. Griffin Young-Ho Jung Junko Shibato Randeep Rakwal Ganesh K. Agrawal Nam-Soo Jwa 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(4):539-554
A metabolomics based approach has been used to study the infection of the Hwacheong rice cultivar (Oryza sativa L. cv. Hwacheong) with compatible (KJ201) and incompatible (KJ401) strains of the rice blast fungal pathogen Magnaporthe grisea. The metabolic response of the rice plants to each strain was assessed 0, 6, 12, 24, 36, and 48 h post inoculation. Nuclear
Magnetic Resonance (NMR) spectroscopy and Gas and Liquid Chromatography Tandem Mass spectrometry (GC/LC-MS/MS) were used to
study both aqueous and organic phase metabolites, collectively resulting in the identification of 93 compounds. Clear metabolic
profiles were observed at each time point but there were no significant differences in the metabolic response elicited by
each pathogen strain until 24 h post inoculation. The largest change was found to be in alanine, which was ~30% (±9%) higher
in the leaves from the compatible, compared to the resistant, plants. Together with several other metabolites (malate, glutamine,
proline, cinnamate and an unknown sugar) alanine exhibited a good correlation between time of fungal penetration into the
leaf and the divergence of metabolite profiles in each interaction. The results indicate both that a wide range of metabolites
can be identified in rice leaves and that metabolomics has potential for the study of biochemical changes in plant-pathogen
interactions. 相似文献
1000.
Tiphaine Dubos Matias Pasquali Friederike Pogoda Lucien Hoffmann Marco Beyer 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(2):239-248
A collection of 55 Fusarium graminearum (Gibberella zeae) strains isolated between 1969 and 2009 in Belgium, Canada, Germany, Italy, Luxembourg, or the USA belonging to the three
known chemotypes (3-acetylated deoxynivalenol, 15-acetylated deoxynivalenol and nivalenol) were screened for their sensitivity
towards the fungicide trifloxystrobin using a liquid culture assay. None of the isolates was completely inhibited by trifloxystrobin
concentrations up to 3 mM. For comparison, prothioconazole completely inhibited fungal growth of a standard isolate at concentrations
as low as 0.007 mM. The maximum level of inhibition, which could be obtained by trifloxystrobin, ranged from 14 to 65% among
the strains tested and was not significantly affected by the country of origin or by the chemotype. The absence of significant
differences in resistance levels between the countries of origin and chemotypes as well as the fact that strains isolated
before the market introduction of strobilurins in 1996 also showed a high level of resistance is evidence that this is largely
a case of natural resistance and not primarily related to strobilurin use in agriculture. 相似文献