全文获取类型
收费全文 | 6180篇 |
免费 | 280篇 |
国内免费 | 1篇 |
专业分类
林业 | 450篇 |
农学 | 139篇 |
基础科学 | 49篇 |
1039篇 | |
综合类 | 1163篇 |
农作物 | 346篇 |
水产渔业 | 388篇 |
畜牧兽医 | 2183篇 |
园艺 | 231篇 |
植物保护 | 473篇 |
出版年
2023年 | 34篇 |
2022年 | 37篇 |
2021年 | 118篇 |
2020年 | 118篇 |
2019年 | 128篇 |
2018年 | 112篇 |
2017年 | 94篇 |
2016年 | 121篇 |
2015年 | 109篇 |
2014年 | 143篇 |
2013年 | 271篇 |
2012年 | 329篇 |
2011年 | 396篇 |
2010年 | 210篇 |
2009年 | 176篇 |
2008年 | 406篇 |
2007年 | 396篇 |
2006年 | 397篇 |
2005年 | 403篇 |
2004年 | 415篇 |
2003年 | 401篇 |
2002年 | 363篇 |
2001年 | 82篇 |
2000年 | 61篇 |
1999年 | 69篇 |
1998年 | 50篇 |
1997年 | 39篇 |
1996年 | 68篇 |
1995年 | 53篇 |
1994年 | 48篇 |
1993年 | 51篇 |
1992年 | 52篇 |
1991年 | 33篇 |
1990年 | 50篇 |
1989年 | 44篇 |
1988年 | 33篇 |
1987年 | 36篇 |
1986年 | 30篇 |
1985年 | 43篇 |
1984年 | 46篇 |
1983年 | 41篇 |
1982年 | 26篇 |
1981年 | 22篇 |
1980年 | 20篇 |
1978年 | 22篇 |
1977年 | 29篇 |
1976年 | 20篇 |
1975年 | 24篇 |
1974年 | 26篇 |
1973年 | 18篇 |
排序方式: 共有6461条查询结果,搜索用时 15 毫秒
941.
942.
943.
944.
Anna Catharina B Berge Elizabeth Thornburg John M Adaska Robert B Moeller Patricia C Blanchard 《Journal of veterinary diagnostic investigation》2008,20(4):497-500
The present study describes antimicrobial resistance patterns of Salmonella enterica subspecies enterica serovar Dublin (S. Dublin) in clinical submissions from calves and temporal and farm-type trends in antimicrobial resistance patterns of the isolates. A total of 300 isolates of S. Dublin were obtained from fecal or internal organs of calves fewer than 120 days of age originating from 84 dairies and 18 calf ranches from July 1998 to December 2002. The isolates were susceptibility tested to a panel of 10 antimicrobials using the disk diffusion assay. Temporal and farm-type trends in individual antimicrobial inhibition zone sizes were assessed and antimicrobial resistance patterns were described using cluster analysis. Isolates obtained from calf ranches compared with dairies exhibited decreased susceptibility to florfenicol, gentamicin, neomycin, sulfisoxazole, sulfamethoxazole/trimethoprim, and tetracycline. During the years 1998-2002, decreasing susceptibility was seen for ceftiofur, enrofloxacin, and sulfamethoxazole/trimethoprim. There were 20 different antimicrobial resistance patterns in the isolate set, indicating that S. Dublin has the ability to transfer and pick up resistance genes with relative ease. The trends seen in antimicrobial resistance in S. Dublin may likely be linked to antimicrobial drug use in young calves. 相似文献
945.
Western blot detection of PrP Sc in archived paraffin-embedded brainstem from scrapie-affected sheep
Robert A Kunkle Eric M Nicholson Semakaleng Lebepe-Mazur Dennis L Orcutt Megan L Srinivas Justin J Greenlee David P Alt Amir N Hamir 《Journal of veterinary diagnostic investigation》2008,20(4):522-526
Scrapie is a naturally occurring fatal neurodegenerative disease of adult sheep and goats, one of a group of mammalian diseases known as transmissible spongiform encephalopathies (TSE) or prion diseases. Immunoassays that identify disease-associated prion protein (PrP Sc) are integral to the diagnosis of scrapie and other prion diseases. Results obtained by either immunohistochemistry (IHC) or Western blot (WB) assay are generally adequate for the definitive diagnosis. Approved or accepted methods for WB diagnosis of TSEs requires the use of fresh or frozen nonfixed tissue samples, whereas formalin-fixed, paraffin-embedded tissue is required for the localization of PrP Sc by IHC. Because disparate processing methods are used for these accepted diagnostic techniques, separate tissue samples are collected from the same animal. Occasions arise in which there is either insufficient quantity of tissue available to complete analysis by both techniques or initial tissue processing is incompatible with one of the assays. Also, results between the assays may differ because of the vagaries of sampling, especially in case material that contains moderate-to-low levels of PrP Sc. The present article describes a method to conduct a WB assay from the same paraffin-embedded brainstem sample used for the IHC diagnosis of experimentally induced sheep scrapie. 相似文献
946.
Dorothea Besselmann Daniela Schaub Christian Wenker Jürg V?llm Nadia Robert Claude Schelling Hanspeter Steinmetz Marcus Clauss 《Journal of zoo and wildlife medicine》2008,39(1):86-91
Since 1956, when the Basle Zoo (Switzerland) initiated the breeding of lesser kudu (Tragelaphus imberbis), 43% of the lesser kudu juveniles died before reaching an age of 6 mo. In this study, the objective was to obtain the pathological findings, nutritional history, and family tree information in order to evaluate the influence of husbandry on juvenile mortality in these animals. The main cause of death was white muscle disease (WMD), diagnosed in 14 cases (26%) of the deceased juveniles. Although enclosure size had remained constant and animal accessibility to the public was constantly high, both herd size and juvenile mortality had increased from 1956-2004. The diet consumed by the whole group in 2004 had deficient levels of vitamin E and selenium. The increasing linear trend of the mortality rate since the 1960s was significant, and there was a significant correlation between herd size and overall juvenile mortality. In contrast, there was no correlation between herd size and the occurrence of juvenile mortality associated specifically with WMD. Other investigated factors (sex, inbreeding, and season) had no significant effect on overall mortality up to 6 mo of age or on mortality associated with WMD. These results characterize both a dietary and a husbandry problem, and are supported by a lack of similar juvenile mortality in another facility where the diet was supplemented with vitamin E, animal numbers were kept low, and the enclosure structure offered more retreat options for the animals. 相似文献
947.
Conservationists, managers, and land planners are faced with the difficult task of balancing many issues regarding humans
impacts on natural systems. Many of these potential impacts arise from local-scale and landscape-scale changes, but such changes
often covary, which makes it difficult to isolate and compare independent effects arising from humans. We partition multi-scale
impacts on riparian forest bird distribution in 105 patches along approximately 500 km of the Madison and Missouri Rivers,
Montana, USA. To do so, we coupled environmental information from local (within-patch), patch, and landscape scales reflecting
potential human impacts from grazing, invasive plant species, habitat loss and fragmentation, and human development with the
distribution of 28 terrestrial breeding bird species in 2004 and 2005. Variation partitioning of the influence of different
spatial scales suggested that local-scale vegetation gradients explained more unique variation in bird distribution than did
information from patch and landscape scales. Partitioning potential human impacts revealed, however, that riparian habitat
loss and fragmentation at the patch and landscape scales explained more unique variation than did local disturbances or landscape-scale
development (i.e., building density in the surrounding landscape). When distribution was correlated with human disturbance,
local-scale disturbance had more consistent impacts than other scales, with species showing consistent negative correlations
with grazing but positive correlations with invasives. We conclude that while local vegetation structure best explains bird
distribution, managers concerned with ongoing human influences in this system need to focus more on mitigating the effects
of large-scale disturbances than on more local land use issues.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
948.
Mariëlle L. van Toor Bart Kranstauber Scott H. Newman Diann J. Prosser John Y. Takekawa Georgios Technitis Robert Weibel Martin Wikelski Kamran Safi 《Landscape Ecology》2018,33(6):879-893
Context
High-resolution animal movement data are becoming increasingly available, yet having a multitude of empirical trajectories alone does not allow us to easily predict animal movement. To answer ecological and evolutionary questions at a population level, quantitative estimates of a species’ potential to link patches or populations are of importance.Objectives
We introduce an approach that combines movement-informed simulated trajectories with an environment-informed estimate of the trajectories’ plausibility to derive connectivity. Using the example of bar-headed geese we estimated migratory connectivity at a landscape level throughout the annual cycle in their native range.Methods
We used tracking data of bar-headed geese to develop a multi-state movement model and to estimate temporally explicit habitat suitability within the species’ range. We simulated migratory movements between range fragments, and calculated a measure we called route viability. The results are compared to expectations derived from published literature.Results
Simulated migrations matched empirical trajectories in key characteristics such as stopover duration. The viability of the simulated trajectories was similar to that of the empirical trajectories. We found that, overall, the migratory connectivity was higher within the breeding than in wintering areas, corroborating previous findings for this species.Conclusions
We show how empirical tracking data and environmental information can be fused for meaningful predictions of animal movements throughout the year and even outside the spatial range of the available data. Beyond predicting migratory connectivity, our framework will prove useful for modelling ecological processes facilitated by animal movement, such as seed dispersal or disease ecology.949.
An exploratory investigation of echocardiographic parameters and the effects of posture on cardiac structure and function in the Livingstone's fruit bat (Pteropus livingstonii) 下载免费PDF全文
Aimee L. Drane Robert Shave Andrew Routh Alberto Barbon 《Veterinary radiology & ultrasound》2018,59(1):89-97
There is growing evidence that dilated cardiomyopathy may be a major cause of death in captive Livingstone's fruit bats (Pteropus livingstonii). Therefore, the primary aim of this prospective, exploratory study was to examine whether a systematic cardiac ultrasound protocol is feasible in this critically endangered species and to report basic measures of cardiac structure and function from a cohort of apparently healthy bats. A secondary aim was to test the effect posture (dorsal recumbency vs. roosting) has upon cardiac function in this species. Transthoracic echocardiograms, including 2D, Doppler, and tissue Doppler measures of cardiac structure and function were completed as part of routine health examinations for bats at a single center (n = 19). Bats were then grouped by age and disease status and the mean and range data reported for each group. In healthy adult bats, with the exception of a reduction in heart rate (P ≤ 0.05), right atrial systolic area (P ≤ 0.05), and right ventricular velocity during atrial contraction, there were no significant changes in cardiac structure or function in response to the roosting position. However, in the bats presenting with dilated cardiomyopathy the current data suggest that left ventricular ejection fraction is improved while roosting. Further work is required to confirm our initial findings, generate diagnostic reference intervals, and explore the causes of dilated cardiomyopathy in this species. 相似文献
950.
Bonginkosi C Gumbi Julie T Shapiro Themba Mahlaba Robert McCleery Duncan MacFadyen 《African Zoology》2018,53(1):23-29
Grazing by large mammals alters vegetation physiognomy, consequently changing habitat suitability for small mammal communities. We investigated the response of terrestrial small mammals to grazing by wild and domesticated ungulates at the boundary of a protected area (Telperion Nature Reserve) and surrounding cattle ranches in Mpumalanga, South Africa over two seasons. Fifteen paired grids were set on either side of the boundary fence at which small mammals were trapped in Sherman live traps placed flat on the ground. A total of 11 760 trap nights resulted in the capture of 187 animals belonging to 14 species (11 rodents, two shrews and one elephant shrew). The small mammal communities in grasslands grazed by domesticated or wild ungulates were similar in abundance, species richness, diversity and demographic parameters, likely due to the fact that vegetation structure of the two grazing systems was also similar. We used generalised linear models to show that rock and grass cover were plausible predictors of small mammal abundance in this system. Rock cover showed a positive relationship with small mammal abundance whilst grass cover showed a negative relationship. Our observations suggest that at the scale of our study and with the current stocking densities, wild and domesticated ungulates have similar impacts on the small mammal community. 相似文献