Differential Response to Salt Stress Within and Among Iranian Melilotus Species

This paper explores the salt-tolerance response of Melilotus species, a forage legume, based on tolerance/susceptibility indices, leaf water relations, leaf/root ion concentrations and multivariate (factor and function discriminate) analyses. In a greenhouse experiment, salinity-susceptibility of 12 accessions of Melilotus (M. albus, M. indicus, M. officinalis) in addition, two controls (alfalfa and Persian clover) were evaluated at 0 mM, 100 mM, 200 mM, and 300 mM of sodium chloride (NaCl). The experiment was conducted in a split plot design with two replications. Stress tolerance score (STS) was calculated based on tolerance/susceptibility indices. Salt-tolerant accessions were identified. An extracted bi-plot based on factor analysis confirmed the results of tolerance/susceptibility indices. There were significant correlations between STS and multivariate statistics. Root potassium cation (K⁺) concentration was higher in Melilotus species than the control species in all the salinity treatments. STS, root K⁺ concentration, leaf turgidity, and leaf succulence characteristics can be used for screening salinity-tolerance in Melilotus accessions. In all treatments, M. officinalis, M. indicus, and M. albus were salinity-tolerant species in descending order.

Abbreviations: Total biomass yield under control (Yp) and stress (Ys) mediums, geometric mean productivity (GMP), leaf area (LA), leaf tissue density (LTD), linear regression coefficient (b), mean productivity (MP), relative water content (RWC%), Succulence (S), specific leaf area (SLA), stress susceptibility index (SSI), stress tolerance index (STI), tolerance (TOL), water content at saturation (WCS), water saturation deficit (WSD), sodium chloride (NaCl), potassium cation (K⁺), sodium cation (Na⁺), analysis of variance (ANOVA), least significant difference (LSD), alfalfa control accession (CHA), Persian clover control accession (CHC), coefficient of variation (CV), statistics of factor (F) and discriminate function (D)     

The effects of foliar applied potassium in the mineral form and complexed with amino acids on pistachio nut yield and quality

A two-year field experiment was conducted to investigate the effects of foliar-applied K in the form of mineral compound (K₂SO₄) and complexed with lysine (KL) and lysine plus methionine (KLMet) with or without ascorbic acid (A) on nut yield and quality of bearing pistachio (Pistachio vera L. cv. Owhadi). Results showed that foliar application of K, regardless of applied source, significantly increased leaf and nut K concentration in comparison with control. In both experimental years, the trees supplemented with K provided higher total yield, fresh and dry mass of shelled and unshelled 100-nut, and 100-kernel dry mass and lower percentage of non-split shells in comparison with those un-supplemented with K. In both years, foliar application of K significantly decreased the percentages of blank nuts. The effectiveness of K-amino chelates in improving nut yield and quality was, in general, higher than that of K₂SO₄. The highest yield and the best nut quality was obtained with foliar application of KLMet-1. The critical leaf and nut K concentration for optimum pistachio nut yield and quality was 2.27 and 1.87%, respectively. Foliar application of K particularly in the form of KLMet-1 during nut filling is recommended to obtain optimum yield and quality of pistachio.     

New strategies for estimation of cut and fill areas in road design with different ground cross-section offsets

Earthwork operations accounted for great amount of forest road construction costs. Any Optimization in planning road profile needs a reliable objective function that calculates earthwork volume in an appropriate time and accuracy. This study aimed at incorporating the shape of the ground cross sections in calculating cut/fill areas using three alternative methods including: Multiple Linear Regression (MLR), Artificial Neural Network (ANN) and heuristic programming in MATLAB environment. The accuracy of results was validated via AutoCAD as a manual method for area calculation of road’s cross-section. Trapezoidal method applied to calculate the area of cut/fill in the heuristic method. Paired sample T-Test and statistical analysis (ANOVA) with confidence level of 0.95 indicated that the heuristic method not only reduced computing time but also provided accurate estimations as same as the manual AutoCAD based method. It can be concluded that although MLR and ANN estimations were as well as AutoCAD results within a reasonable time, they need to be retrained by adding, removing or changing entrance parameters.     

Geostatistical analysis of soil mechanical properties in Ardabil plain of Iran

This study was conducted to estimate the spatial distribution of penetration resistance (PR), Proctor maximum bulk density (MBD) and critical water content (CWC) as soil mechanical indices. Soil samples to determine sand, silt, clay, organic carbon (OC), CaCO₃, bulk and particle density, total porosity, field water content, MBD and CWC values were collected. Field measurements of PR at 0–10 cm depth were taken from 105 geo-referenced points with 3000 × 3000 m intervals in agricultural lands of Ardabil plain, Iran. Ordinary kriging (OK) and inverse distance weighting (IDW) methods were used to analyze spatial variability of PR, MBD and CWC. The strongest spatial dependences with the lowest ranges of influence were found for OC (7560 m) and MBD (8370 m). The models of fitted semivariograms were Gaussian for PR and MBD, and exponential for CWC. The moderate spatial dependences with the ranges of 13,300 and 40,100 m were found for CWC and PR, respectively. The best prediction according to Lin’s concordance correlation coefficient was obtained by OK for PR (0.48) and IDW for MBD (0.09) and CWC (0.03). These results can be applied in programming of optimum tillage operations for reducing soil compaction risk in the studied region.     

Effects of melatonin on oocyte maturation in PCOS mouse model

The purpose of oocyte in vitro maturation is generation of mature oocytes that could support future development. Efforts have been made to enhance oocyte developmental competence by developing optimal culture conditions. The present study is conducted to determine melatonin effects on quality of polycystic ovarian syndrome (PCOS) oocytes when it has been added during in vitro maturation, and immature oocytes were cultured in defined conditioned medium with and without different melatonin concentrations. Melatonin could significantly improve nuclear maturation of PCOS oocytes (81.1% vs. 56.3%, P < 0.05 were achieved with 10^?6 mol/L concentration). Cleavage rate was significantly higher in 10^?5 mol/L concentration compared to untreated oocytes in PCOS (54% vs. 35%, respectively) and it was significantly higher with 10^?6 mol/L concentration in the control group, 55% versus 38%, compared to untreated oocytes. This study showed that melatonin has the potential to induce oocyte nuclear maturation and guarantee fertilization potential. © 2016 Japanese Society of Animal Science     

Evaluation of <Emphasis Type="Italic">Aloe vera</Emphasis> Gel as an Alternative Edible Coating for Peach Fruits During Cold Storage Period

Application of bio-based edible coatings, which are in line with sustainable agriculture practices, has been a hot topic in recent years. In order to evaluate the effect of Aloe vera L. gel coating, on the store ability of peach fruits, a 30-day postharvest experiment with 10-day sampling intervals was performed. A. vera gel coating had significant positive effects on the weight loss, color change, and sensory evaluation. The amount of weight loss, color change, Total Soluble Solids (TSS) and Titratable Acidity (TA) in coating was lower than control. Furthermore, our results showed that A. vera gel coating can enhance visual properties, and could also lead in more favourable taste and texture. Regarding the obtained results, it can be suggested that A. vera gel coating can enhance the qualitative and quantitative post-harvest traits of fruits, hence, it could be a suitable alternative for chemicals preservative in commercial storage of peach fruits.     

Distribution changes of woody plants in Western Iran as monitored by remote sensing and geographical information system: a case study of Zagros forest

The status of woody plants in dry-land systems is a fundamental determinant of key ecosystem processes. Monitoring of this status plays an important role in understanding the dynamics of woody plants in arid and semi-arid ecosystems. The present study determined the dynamism of the Zagros forests in Iran using Remote Sensing and Geographic Information System techniques and statistical science. The results show that the density of trees varied from 10 to 53 % according to the physiographic and climatic conditions of semi-arid regions. The best and lowest correlation between vegetation indices and forest density were obtained for the global environmental monitoring index (GEMI; R ² = 0.94) and soil adjust vegetation index (R ² = 0.81), respectively. GEMI is used to monitor land use changes over a 10-year period. Results show that 2720 ha² of forest have been destroyed by human interference and tillage on steep slopes during this period which also resulted in the loss of the fertile soil layer. GEMI determined the areas with a biomass of trees and could normally separate border regions with low biomass density of trees from regions without canopy cover. The results revealed that assessment of forest and vegetation cover in arid and semi-arid arduous forest regions using satellite digital numbers and ordinary sampling is subject to uncertainty. A stratified grouping procedure should be established to increase the accuracy of assessment.     

Effects of different salinity and temperatures on the growth,survival, haematocrit and blood biochemistry of Goldfish (Carassius auratus)

The aim of the present study was to investigate the effects of different salinities (0‰, 6‰ and 12‰) and temperatures (23, 27 and 31 °C) on the food consumption, growth, blood biochemistry and haematocrit of Goldfish. After 45 days of exposure to different salinities and temperatures, Goldfish showed a good adaptation to these salinities and temperatures in terms of blood biochemistry (glucose and triglyceride) and haematocrit. Salinities (0‰ and 6‰) and temperatures (23 and 27 °C) did not affect the weight gain, specific growth rate, final biomass and feed conversion rates, but these parameters were significant (P<0.05) at 12‰ salinity and 31 °C temperature. Plasma total protein levels decreased with the increase in salinity (P<0.05), while they were independent of temperature. In conclusion, Carassius auratus, a freshwater stenohaline fish, showed good growth in saline waters with maximum 12‰ salinity and 31 °C temperature.     

Brainstem removal using compressed air for subsequent bovine spongiform encephalopathy testing

The use of compressed air to expel the obex through a hole in the skull generated using a captured bolt stunner. The obex is the part of the brain that is tested for bovine spongiform encephalopathy.