Impact of cold-season grazing and supplementary feeding on rumen metabolic parameters and micro-organisms in the ewes of Gansu Alpine Fine Wool Sheep北大核心CSCD

An experiment was conducted to scientifically identify the appropriate feed for Gansu Alpine Fine Wool ewes in order to change the animals' breeding mode, which currently can only be mated at the age of 2.5 years due to insufficient nutritional supply. The experiment selected 48 12-month-old ewes and randomly divided them into group a (Diet 1), group b (Diet 2), group c (Diet 3), and a control group with no supplementary feeding. The feed test was run for 90 days. The rumen pH of groups a and b were very significantly higher (P<0.01) or group c significantly lower (P<0.05) than control group. The rumen ammonia nitrogen, protein nitrogen and total nitrogen of groups a, b and c were all higher than control group (P<0.01). The total volatile fatty acids of group a and b were all higher than the control group (P<0.01), group b significantly higher than group c (P<0.05). The molar ratio of acetic acid and propionic acid in group a was very significantly higher than control group (P<0.01), group b and c were significantly higher than control group (P<0.05). The molar ratio of butyric acid in group a was very significantly higher than group c (P<0.01). The ratio of acetic acid and propionic acid in group b was significantly lower than control group (P<0.05). The number of several rumen fiber-degrading bacteria and protozoa in groups a, b and c were very significantly (P<0.01) or significantly (P<0.05) higher than the control. In conclusion, this study shows that supplementary feeding can positively affect the rumen metabolic parameters and the number of several rumen microorganisms in Gansu Alpine Fine Wool ewes. In general, the effects of Diet 1 were the strongest, while Diet 2 also produced notable improvements. © 2018, Editorial Office of Acta Prataculturae Sinica. All rights reserved.     

植酸酶对不同类型的断乳仔猪日粮粗蛋白、氨基酸和能量的消化率的影响

通过对4个安装了简易T型瘘管的断奶仔猪研究表明,添加植酸酶并没有提高玉米-豆粕型日粮(试验组1)、小麦-豆粕型日粮(试验组2)和大麦-豌豆-菜籽粕型日粮(试验组4)粗蛋白和氨基酸的回肠表观消化率(AID),而使小麦-豆粕-菜粕型日粮的粗蛋白和氨基酸回肠表观消化率或粗蛋白和消化能的总消化道表观消化率(ATTD)得到了显著的提高(P<0.05)或呈提高的势态(P<0.10)。这些结果表明,氨基酸对细菌性植酸酶的反应因子取决于日粮的组成。     

粪肥施用对土壤团聚体的影响——Meta分析

粪肥施用为土壤输入大量有机质与养分,能影响土壤团聚体的形成和稳定性,大部分研究主要认为粪肥施用促进土壤大粒径团聚体的形成(marcoaggregate,≥0.25 mm),但进一步细分是对大团聚体(large marcoaggregate,>2 mm)还是小团聚体(small marcoaggregate,2 ~ 0.25 mm)的影响更大,研究存在较大差异。本文使用Meta分析收集了36篇相关文献,共267个团聚体数据,发现施用粪肥能显著增加土壤大团聚体和小团聚体含量,分别增加了95% 和17%,同时减少了土壤微团聚体(microaggregates,0.25 ~ 0.053 mm)和土壤粉黏颗粒(silt-plus and clay-size particles,<0.053 mm)含量,分别减少了14% 和20%。本文还从土壤因素、粪肥因素、气候因素3个角度的解释变量来分析粪肥施用对土壤团聚体影响不同的原因,结果发现土壤类型、土壤pH、粪肥种类和年均气温、年均降水量是造成不同研究结果差异的主要原因。     

以色列野生二棱大麦农艺性状差异及相关性分析

为给野生大麦的遗传和基因资源的研究提供依据,对起源于以色列不同地区6个群体的16个生态型野生二棱大麦的物候性状进行了研究.结果表明,不同生态型间的物候性状差异明显.从个体水平上分析,其最大值均出现在湿生生态型中,除旗叶面积外,其最小值则出现在旱生生态型中;从群体水平上分析,除每穗粒数外,湿生型平均值皆高于旱生型平均值.曼-怀氏等级和检验显示,湿生型和旱生型之间的这些物候性状差异达到显著水平.斯皮尔曼秩相关性检验则表明,9个物候与性状间,除三叶期与粒重、穗粒重无相关性外,其余物候性状间均呈显著的相关性,其中生长期与旗叶面积、株高、穗长、芒长等呈显著正相关,与每穗粒数则呈显著负相关;芒长与旗叶面积、株高,稳长与株高存在显著正相关,而每穗粒数则与芒长和旗叶面积分别呈显著负相关,芒长与穗长亦呈显著正相关.分析表明,以色列不同地区野生大麦物候性状差异是其生境主导下生存选择的结果,其丰富的表现型多样性,可用于遗传与育种研究.     

Calcium oxalate accumulation and soil weathering in mats of the hypogeous fungus Hysterangium crassum

Fungal mats of Hysterangium crassum (Tul. and Tul.) Fischer occupied a mean of 9.6% of the upper 10 cm of soil developed under a 40–65 yr old stand of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) in Oregon. This hypogeous basidiomycete exudes large amounts of oxalic acid, some of which precipitates with Ca in microscopic crystals of calcium oxalate, resulting in a mean CaC₂O₄ content of 82g m^?2 for the entire soil. Soil oxalate concentration was significantly greater within fungal mats (P < 0.01) and soil pH was significantly lower (P < 0.01) than in soil adjacent to mats. The quantity of Ca present as CaC₂O₄ is 0.5 the amount of exchangeable Ca in the soil and exceeds the mass of Ca lost annually in runoff. Scanning electron micrographs show intense chemical weathering, attributable to oxalate attack, in the immediate vicinity of hyphae. X-ray diffraction patterns of clay indicate bulk weathering is more intense within the fungal mats than in adjacent uncolonized soil.     

The evaluation of piromidic acid as an antibiotic in fish: an in vitro and in vivo study

Abstract. The activity of piromidic acid (PA, 8-ethyl-5, 8-dihydro-5-oxo-2-prior lidinopyrido [2,3–d] pyrimidine-6-carboxylic acid) against bacteria in fish was evaluated in vitro and in vivo .
In vitro , PA was effective against a wide range of pathogenic bacteria stocked in this laboratory and freshly isolated from fish. PA was active against organisms showing multiple drug-resistance and showed no cross-resistance to chloramphenicol, tetracycline and sulfamonomethoxine.
In vivo , orally administered PA was effective against experimental infections with Aeromonas hydrophila and Vibrio spp. in goldfish and eels. The efficacy of PA against these infections was equal to or higher than that of chloramphenicol, and higher than that of tetracycline. Distribution of PA in the Ash was investigated by bioassay and autoradiography. In the bioassay, orally administered PA was easily absorbed and distributed in the blood and tissues of major organs within 1 h after the administration. Peak levels were attained 2 to 4 h post dosing, and no residue was detected in the blood and tissues 48 h post dosing. The results obtained from the autoradiographic investigation in goldfish were similar to those from the bioassay. Therapeutic levels of PA were well tolerated by fish when administered with feed.     

Metabolism of fluorodifen by soil microorganisms

The metabolism of fluorodifen (p-nitrophenyl α,α,α,-trifluoro-2-nitro-p-tolyl ether) by soil microorganisms in the presence or absence of other carbon and nitrogen sources was studied. The degradation of this herbicide continued for 5 days, when benzoate or acetate and ammonium sulphate were included in the cultures, and for more than 5 weeks when fluorodifen was used as a sole source of carbon and nitrogen.Under all conditions nitrite ions were produced at concentrations ranging between 5 and 80 per cent of the nitro-nitrogen of the fluorodifen present. The highest concentration of nitrite was obtained when added carbon sources were used with fluorodifen. The lowest nitrite concentration accumulated when the fluorodifen was used as sole source of carbon and nitrogen. The nitrite reached a maximum value after a few days of incubation, followed by rapid disappearance.p-Nitrophenol and quinol were identified in the acid-ether extract of cultures. It is suggested that the first step in the degradation of fluorodifen is the hydrolysis of the ether linkage followed by the direct elimination of the nitro-groups as nitrite ions.