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51.
In recent years, worldwide socioeconomic, ecological, and cultural changes have led to a paradigm shift in forestry and forest management. Some years ago, most forest enterprises exclusively focused on wood production, whereas today, forest management is expected to be multifunctional and economically as well as socially and ecologically sustainable [Harrison, S., Herbohn, J., Niskanen, A., 2002. Non-industrial, smallholder, small-scale and family forestry: what's in a name? Small-scale Forest Economics. Management and Policy 1 (1), 1–11; Schmithuesen, F., 2004. Forest policy developments in changing societies — political trends and challenges to research. Towards the sustainable use of Europe's forests: Forest ecosystems and landscape research: Joensuu: European Forest Institute, Proc., vol. 49, pp. 87–99]. In this context, non-timber forest products (NTFPs) such as forest honey, mushrooms, or berries could gain importance as an additional source of income for the forestry sector. To assess the market potential of NTFPs in Switzerland, two corresponding surveys were conducted. A consumer survey (N = 897) conducted in six cities in Switzerland investigated the purchase of Swiss NTFPs, consumer preferences, and demand potentials. An additional survey of sellers of NTFPs (N = 105) looked at these aspects from a complementary perspective. The results showed that there is a high esteem and considerable demand potential for such products among urban consumers. Food products were mentioned most frequently. Both consumers and sellers considered quality and environmental friendliness the most important attributes of NTFPs. Structural and organizational innovations and considerable marketing efforts and sales promotion would be required to expand the market for Swiss NTFP, however. Our results indicate that certification of the products' origin, environmental performance, and quality could support such efforts and that recreational and ecological forest services have a synergetic potential for the marketing of NTFPs.  相似文献   
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Sexual reproduction in fungi is controlled by mating type genes, which are located at the MAT locus. In this study, we investigated the structure of this locus in the phytopathogenic fungus Phyllosticta citricarpa, the causal agent of citrus black spot disease. Despite intensive study, its sexual state has never been observed in single-spore culture. Through analysis of the genome sequences of two individual P. citricarpa isolates, the sequence of the DNA lyase gene was identified and, as previously reported in the literature, the mating type genes were located in the 3′ flanking region of this gene. The results suggested that P. citricarpa is heterothallic, owing to the exclusive presence of the MAT1–1 or MAT1–2 gene in individual strains. In order to characterize the MAT locus, we designed primers to amplify this region. P. citricarpa was found to have complete and apparently functional copies of MAT genes, containing α-1 and HMG domains, present in different isolates. In addition to MAT1–2-1 and MAT1–1-1 genes, the MAT1–1-4 gene was located in the 5′ flanking region of the MAT1–1-1 gene and the MAT1–2-5 gene was located in 5′ flanking region of the MAT1–2-1 gene. A multiplex PCR protocol was also developed to differentiate P. citricarpa idiomorphs, which can be used in distribution and incidence studies of mating type strains, in order to determine the occurrence of sexual reproduction and to facility crossing studies. Furthermore, in Brazil, the two idiomorphs occur in a 1:1 ratio, which is expected in sexually reproducing populations.  相似文献   
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Water, Air, & Soil Pollution - Elevated radium (Ra) concentrations have been observed in aquifers with high naturally occurring salinity. The flux of radon (Rn) gas from the decay of Ra out of...  相似文献   
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Atopic dermatitis (AD) is a chronic or chronically relapsing human and canine skin disease that is known to affect the quality of life (QoL) of affected individuals. Several studies have been conducted to develop disease-specific questionnaires and assess QoL in parents of children with AD and in the children themselves. The severity of canine AD is however currently evaluated using only clinical and pruritus scores. Measurement of the QoL of affected dogs and their owners could therefore provide a new tool for assessing disease severity and treatment efficacy. Ninety-eight owners of AD-affected dogs were asked to complete two questionnaires aiming to evaluate the QoL of affected dogs and their owners on one hand and the relationship between them and their dog on the other hand. Statistical analyses were carried out in order to assess the validity of the questionnaires and to select relevant questions for future studies. These analyses resulted in the selection of 13 questions that could be used in further studies aiming at determining the QoL of affected animals and their owners.  相似文献   
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The IgE specific Fc-? receptor test is widely used for serological evaluation of allergic canine patients. Aim of the study was to evaluate intra- and interlaboratory variability in three independent laboratories. Duplicate serum aliquots from 15 atopic dogs were submitted simultaneously to 3 laboratories (LA, LB, LC), which subsequently performed the test in a blinded fashion. LA and LC analysed sera for 35 allergens and expressed the results as optical density units (OD), while LB analysed 15 allergens and provided reaction grades (RG). Results were compared with three factorial ANOVA. Intralaboratory variability was evaluated by calculating the dispersion factor for data of LA and LC and standard deviation (SD) for LB. Correlation coefficients (r) were used to evaluate interlaboratory variability. Depending on the allergens, intralaboratory dispersion factor for LA and LC varied from 1.15 to 6.63 and 1.19 to 6.17, respectively. In LB the SD varied between 0 and 0.6 RG. Regarding interlaboratory variability, correlation of OD values between LA and LC was excellent (r > 0.8, p < 0.001) for 13 of 35 allergens. There was a significant difference in OD results for 9 allergens. The results were significantly correlated (r > 0.8, p < 0.001) for all except 3 allergens (Dermatophagoides pteronyssinus, mixed grasses, nettle). As far as negative/positive results were concerned, intralaboratory differences were 3.14% and interlaboratory differences were 4.76%.  相似文献   
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Bioassays were conducted in Mauritania to determine the toxicity of botanical insecticides from the tree Melia volkensii Gürke (Meliaceae) to ladybird predators (Coleoptera: Coccinellidae) of the date palm scale, Parlatoria planchardi Targ. (Homoptera: Diaspididae). M. volkensii seed extract was formulated in neem oil or a mixture of neem and maize oil. Three preparations were tested on Chilocorus bipustulatus L. var. iranensis, an introduced species, and one on the indigenous Pharoscymnus anchorago F., a species already used in previous bioassays. Fourth instar larvae were exposed for 2 days to treated scale-infested date palm leaves. The botanical insecticides were toxic to C. bipustulatus. Median lethal application rates (LR50s) were close to the recommended application rate of 1 l/ha. In contrast, P. anchorago showed no increased mortality at this rate. Hazard quotients (application rate divided by the LR50) were generally less than 2, suggesting a low risk for both species. However, risk mitigation measures are recommended when using oil formulations because the threshold value for C. bipustulatus, the more susceptible of the two ladybird species, would be exceeded at higher dose rates or when conducting multiple applications. Sublethal effects included an extension of the larval stage and morphogenetic defects. These effects were again more pronounced in C. bipustulatus than in P. anchorago.  相似文献   
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Background: In veterinary medicine, there is increasing interest in measuring C‐reactive protein (CRP) as a tool for diagnosis and monitoring of inflammatory diseases. Reported CRP concentrations for healthy dogs have ranged from 0 to 8.9 mg/L. Objectives: The aims of this study were to evaluate a canine‐specific point‐of‐care (POC) lateral flow immunoassay for qualitative CRP measurement in healthy and diseased dogs and to compare results with those obtained by a quantitative ELISA. Methods: Blood samples from 73 client‐owned dogs were available for testing: 16 healthy dogs and 57 dogs with a variety of infectious, inflammatory, or neoplastic diseases. CRP was measured in heparinized whole blood samples and serum with the TECOmedical Dog CRP‐visual POC test. A red line develops in the POC device if CRP is ≥5 mg/L, and results are scored as negative or positive. An ELISA validated previously for canine serum was used as the reference method. Results: For all dogs, serum CRP concentrations measured by the ELISA ranged from 0.1 to ≥350 mg/L (median=38 mg/L). Percentages of the CRP POC test results that agreed with the ELISA results were 98.6% for whole blood and 97.3% for serum samples. For serum samples, sensitivity of the POC test was 96.4% and specificity was 81.3%. For whole blood, sensitivity was 94.7% and specificity was 93.8%. Conclusions: The POC test had very good agreement with the ELISA test and had high sensitivity and specificity; therefore, it can be used as a qualitative test to screen for increases in CRP concentrations.  相似文献   
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