Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas

ABSTRACT

In the present study, we reported the isolation and molecular characterisation of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing endophyte and its effect on alleviating drought stress in maize. Ten morphologically distinct indigenous plant growth-promoting rhizobacteria (PGPR) from maize roots and rhizosphere were screened for multiple plant growth-promoting (PGP) traits, and of ten, two strains (DHK and B1N1) possessing maximum PGP traits were evaluated at three water stress regimes in maize: (i) 80% field capacity (FC), (ii) 60% FC, and (iii) 40% FC for 45 days, starting 15 days after sowing to the maturity. Inoculation of DHK strain with maize at 80% FC induced a significant increase in plant biomass (root biomass, 86.67%; shoot biomass, 111.63%) together with decreased reactive oxygen species and increased activity of antioxidant enzymes (superoxide dismutase, 14.11?U/g fresh weight; peroxidase, 1.14?U/g fresh weight and catalase; 14.91?U/g fresh weight) compared to uninoculated control at same water regime (80% FC). The derivative strain also significantly accrued nutrients in maize and enhanced total chlorophyll and amino acid content in comparison with non-inoculated plants. 16S rDNA analysis of DHK strain revealed its lineage to Bacillus subtilis. Present investigations demonstrated the potential of the bacterial partner in alleviating drought stress in maize.     

Long-Term Organic Amendment Application Improves Influence on Soil Aggregation,Aggregate Associated Carbon and Carbon Pools under Scented Rice-Potato-Onion Cropping System after the 9th Crop Cycle

A field experiment was conducted to investigate the influence of long-term application of organic manures on aggregate stability, associated carbon concentrations and carbon pools as an important soil-quality parameter under a scented rice-potato-onion cropping system in silt-loam textured soil in Eastern Bihar, India (subtropical climatic condition). Five treatments were used: 1) nitrogen, phosphorus and potassium (NPK) – 100% recommended dose of NPK; 2) NPK+FYM (farmyard manure) – 50%NPK+50% N as FYM; 3) FYM+VC (vermicompost)+ NC (neem cake) – different organic sources each equivalent to 1/3 of the recommended N (FYM +VC +NC); 4) FYM+VC+NC+PSB (phosphate-solubilizing bacteria – different organic sources each equivalent to 1/3 of the recommended N (FYM +VC+NC)+biofertilizers containing N and P carriers (PSB) and 5) FYM+BFN+BM+PSB – 50% N as FYM + biofertilizer for N +Bone meal to substitute P requirement of crops + PSB. The aggregate size distribution (>250 μm) at different soil depths is higher in treatment FYM+VC+NC+PSB and is at par with FYM+VC+NC, followed by FYM+BFN+BM+PSB and NPK+FYM, and the lowest in treatment receiving 100% NPK. The mean carbon concentration in each soil fraction was higher in soil depth 0–10 cm, followed by 10–20 cm and 20–30 cm. The trend of mean weight diameter (MWD) in different treatments was FYM+VC+NC+PSB ≥ FYM+VC+NC > NPK+FYM > FYM+BFN+BM+PSB > NPK. The trend of carbon concentrations in different treatments was FYM+VC+NC+PSB ≥ FYM+VC+NC > FYM+BFN+BM+PSB ≥ NPK+FYM > NPK. Non-labile pool of carbon forms the major portion (60.14%) of the total soil organic carbon (SOC) irrespective of all depths. Bulk density (BD) has a significant role in stabilizing soil aggregates as well as increasing the SOC content in soil. SOC was negatively correlated with BD (r = ?0.870, p = 0.05), MWD (r = ?0.911, p = 0.01) and geometric mean diameter (GMD) (r = ?0.958, p = 0.05) irrespective of depth. This study took further steps toward understanding the enhancing of aggregate stability on organic manures addition for soil quality improvement.     

Influence of foliar application of mineral nutrients at different growth stages of guava

Foliar application of nutrients enhances efficient use along with quick response. The experiment was undertaken to study the effect of foliar application of mineral nutrients viz., boron (B), zinc (Zn), calcium (Ca), and potassium (K) sprayed at different growth stages viz., at fruit set and at two weeks after fruit set of guava (Psidium guajava L.). Increase in plant canopy and minimum seed index was in Zn (0.03%) sprayed at fruit set. Fruit size, sugar: acid, and organoleptic score were better in K (0.5%) sprayed at two weeks after fruit set. Increased yield in Zn (0.01%) and pectin enhancement with B (0.03%) was found at two weeks after fruit set, respectively. Minimum physiological loss in weight of fruits was with Ca (0.5%) sprayed at two weeks after fruit set. In general, for most of the desirable characters 0.5% potassium (K) spray at 2 weeks after fruit set had better results.     

Transfer of 137Cs and 40K from Agricultural Soils to Food Products in Terrestrial Environment of Tarapur, India

The ¹³⁷Cs and ⁴⁰K activities and transfer factors from soil to vegetables, grass, and milk from villages located around Tarapur Atomic Power Station (TAPS) were determined using high-resolution gamma spectrometry. A total of 32 soil, 21 vegetable, 23 dry paddy grass, and 23 milk samples were collected from 23 different agricultural farms from various villages around TAPS to determine transfer factors for natural environment. The mean concentration values for ¹³⁷Cs and ⁴⁰K in soil, grass, and milk were 2.39?±?0.86 Bq kg^?1, 0.31?±?0.23 Bq kg^?1, and 12.4?±?5.7 mBq L^?1 and 179?±?31 Bq kg^?1, 412?±?138 Bq kg^?1, and 37.6?±?9.3 Bq L^?1, respectively, for soil?Cgrass?Cmilk pathway. In the soil?Cvegetation pathway, the mean concentrations values for ¹³⁷Cs and ⁴⁰K were 2.15?±?1.04 Bq kg^?1, 16.5?±?7.5 mBq kg^?1, and 185?±?24, 89?±?50 Bq kg^?1, respectively. The evaluated mean transfer factors from soil?Cgrass, grass?Cmilk, and soil?Cvegetation for ¹³⁷Cs were 0.14, 0.0044, and 0.0073 and that of ⁴⁰K were 2.42, 0.0053, and 0.49, respectively. Only 15 out of total 44 milk and vegetable samples were detected positive for ¹³⁷Cs, indicating a very low level of bioavailability.     

Transgene integration - an analysis in autotransgenic Labeo rohita Hamilton (Pisces: Cyprinidae)

Transgenic Labeo rohita founder population was analyzed for the presence of autotransgene having histone 3 promoter and growth hormone (GH) cDNA (LRH3-GHcDNA) or total GH gene (LRH3-GH2.8) by PCR with transgene specific primers. Transgene specific amplification was seen with LRH3-GHcDNA in five out of seven individuals and all three fishes with LRH3-GH2.8, indicating their transgenic nature. Transgene integration was also studied by Southern hybridization of DNA isolated from blood of the transgenic fishes with two different probes (histone 3 promoter and cDNA of L. rohita). Autotransgene integration was confirmed in all PCR positive transgenic individuals. The site of integration of the transgene in the genome of the four transgenic fish could be determined by inverse PCR. Two individuals showed integration at the same site whereas in the remaining two individuals the integration sites were different.     

Yield characteristics and morphometric relationships of giant freshwater prawn,Macrobrachium rosenbergii (de Man, 1879)

Meat yield of 233 individuals of giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879), and their morphometric relationships were examined. Morphometric parameters of different length and weight measurements were recorded for both the sexes separately and subjected to analysis. The meat yield of prawns was size and sex dependent with females showing significantly greater (P < 0.05) tail meat yield than males. The tail meat as a body weight percentage ranges from 26.12 to 40.75 and 32.97 to 42.11 in males and females, respectively. Much of the differences in yield are related to the size and weight of the head and claws. The head weight of males was observed to be higher compared to females in all the size classes. Regression slopes of total length versus body weight and total length versus head weight were significantly higher than critical isometric value indicating high positive allometric growth in both the sexes, with males showing significantly higher (P < 0.05) slope than females. The relationship parameters of total length versus tail weight and total length versus tail meat showed a positive allometric growth in females, whereas it is isometry and negative allometry in males. The morphometric relationship of tail length versus all weight measurements showed positive allometry and negative allometry in females and males, respectively.     

Preparation of color filters using azo and nitroso colorants

Color filters were prepared by using azo colorants and nitroso metal complex colorants. Initially, crushed glass particles were colored by using the azo and nitroso-iron complex colorants. These colored glass particles were used to obtain a colored composite with unsaturated polyester resin to give colored glass filters. Glass filters obtained were used for study to evaluate fading rate with respect to UV radiation, heat and moisture. Thermo gravimetric analyses of synthesized colorants were also carried out to determine their thermal stability.     

Photoilluminance of different woven structures by treatment with phosphorescent pigment

The work is focused on study of photoilluminance of woven fabrics. Plain, twill, and matt woven structures are investigated after application of the phosphorescent agent. Samples were kept under observation for different periods of time in completely sealed box where light couldn’t enter from any side. Lux meter was used to measure the intensity of light. As the charging time increases, glowing time also increases and the weave structure having greater capacity to absorb light radiations from the source, glows for a longer duration of time. The weave having larger number of intersections facilitates greater absorption of light photons and the weaves having relatively lower number of intersections have a smaller capacity to absorb light radiations. Longer yarn floats in the fabric, tend to reflect higher portion of light, which in turn causes less charging of the phosphorescent fabric. Thus a shorter glow time is observed. Tensile and tear strength was mesaured for all fabric samples before and after application of phosphorescent pigment so as to observe the effect of treatment on mechanical properties.     

Experimental investigation of mechanical behavior of Jute-Flax based glass fiber reinforced composite

Present technological development and innovation needs a better class of material that meets all the practical applications along with its environmental friendly nature and economical value. Hybrid natural fiber composites, a sector of natural composites meets these requirements. This paper deals with fabrication, mechanical characterization of a hybrid (Jute+Flax+GFRP) composite and also the comparison of it with the (Jute+GFRP) based composite. These composites are fabricated using hand lay-up technique. The arrangement of hybrid composite is such that a layer of vertically laid flax fiber is flanked between layers of horizontally laid jute fiber. Epoxy resin alongside with HY951 hardener is used as the binding agent throughout the layer. Glass fiber laminates are used on both sides for improving the surface finish and surface hardness. The volumetric fraction is such that one third of total volume is occupied by Jute and Flax fibers. Test results shows that the hybrid natural composite has excellent properties under tensile, flexural loading. At last failure morphology analysis is done using Scanning Electron Microscope (SEM) and the internal structure of the broken specimen is discussed.