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101.
为揭示鄱阳湖湿地苔草群落的构建机制,选择灰化苔草(Carex cinerascens)群落作为研究对象,通过设立3条样带调查不同水分梯度的群落物种多度,选用断棍模型(BSM)、生态位优先占领模型(NPM)、优势优先模型(DPM)、随机分配模型(RAM)和生态位重叠模型(ONM)5个生态位模型对群落种-多度关系进行拟合,结果表明,1)灰化苔草群落可按土壤水分划分为高湿度、中湿度和低湿度3组,分别对应从湖边、湿地中间和湿地边缘的梯度分布;2)从高到低的水分梯度上,灰化苔草群落的物种数先增加后降低,优势种多度逐渐增加,物种多度分布曲线由相对平缓变为陡峭;3)高湿度灰化苔草群落物种多度分布格局的最佳拟合模型为BSM,中、低湿度的理想模型转变为NPM。研究认为,水分条件的变化是灰化苔草群落物种多度分布格局改变的主因,随着水分梯度降低,群落构建的生态学过程由随机生态位变为生态位优先占领。研究为鄱阳湖湿地多样性保育和生态功能管理提供了理论参考。 相似文献
102.
利用手性衍生化试剂L-薄荷醇和气相色谱柱前衍生化,将瘤胃液和青贮液中L-和D-乳酸对映体转化为酯类非对映体对,建立同时分析发酵液中乳酸对映体和挥发酸的气相色谱检测方法。样品乳酸对映体的质量浓度在0.05~10.00 mg/mL范围内时,标准曲线的决定系数分别达到R2=0.999 5和R2=0.999 6。乳酸对映体的回收率为96.15%~108.24%,且相对标准偏差小于5%。采用本方法检测瘤胃液和青贮液样品的L-和D-乳酸对映体含量,并且与酶学检测法进行比较,2种方法的测定结果没有显著性差异(P>0.05)。发酵液中挥发酸经衍生化处理,可以与L-和D-乳酸对映体在同一色谱条件下检测,各组分在0.05~5.00 mg/mL范围内线性良好,R2=0.998 2~0.999 4。乳酸对映体和挥发酸的色谱分析在6 min完成。 相似文献
103.
Improved protocol for Agrobacterium mediated transformation of tomato (Lycopersicon esculentum) Micro-Tom was developed to use in corporation of the carotenoid biosynthetic genes CsZCD (Crocus zeaxanthin 7,8-cleavage dioxygenase). From these experiment, a transformation methodology using explants from cotyledons cultured for 1 day on the medium with zeatin 2 mg/L, IAA 0.1 mg/L, carefully submerged in the Agrobacterium inoculum for 20 min, then concultured with the agrobacterium for 3 days on the same medium, followed by a transfer to the same medium with 500 mg/L cefotaxin for 3 days and then by a transfer to the same medium with 100 mg/L kanamycin and 500 mg/L carabenillin for 6–8 weeks and resulted in a greater than 20% transformation efficiency in the concentration of Agrobacterium OD600 = 0.2 tested. In this transformation method, no feeder layers were used and the subculture media was minimal. Among the Agrobacterium concentrations of OD600 = 0.2, 0.5 and 1.0, the best transformation efficiency, 20.87%, was obtained by using OD600 = 0.2, which was significantly higher than that of OD600 = 1.0. The presence of the inserted target genes was checked using a rapid and efficient PCR test. The protocol was successfully employed in the production of transgenic Micro-Tom tomato containing the carotenoid biosynthesis CsZCD under constructive promoter. This procedure represents a simple, efficient and general means of transforming tomato. 相似文献
104.
LI Yao WEI Xin-yi YAN Jun-li WANG Mo WU Dao-qi ZHANG Gao-fu YANG Hai-ping LI Qiu 《园艺学报》2017,33(2):308-314
AIM: To investigate the aggravating effect of albumin overload on the kidney injury induced by lipid nephrotoxicity, and to observe the renoprotective effect of simvastatin (SIMV) on adriamycin nephropathy (ADR) mice.METHODS: SPF healthy male BALB/c mice were randomly divided into control group, ADR group, ADR with bovine serum albumin (BSA) overload (ADR+BSA) group, and ADR with both BSA overload and SIMV treatment (ADR+BSA+SIMV) group. All mice were uninephrectomized under general anesthesia 2 weeks before setting up ADR model. ADR+BSA model started to be set up 4 weeks later. At the end of the 0th, 2nd, 6th, 10th and 14th weeks, 24 h urinary protein was evaluated. At the end of the 14th week, the serum biochemical indexes and the kidney pathological changes were observed, and glomerulosclerotic index (GSI) was also evaluated. The cholesterol in the kidney was measured by enzymic colorimetric method and oil red O staining. The expression of IL-1β, TGF-β1 and low-density lipoprotein receptor (LDLr) in the kidney tissues was determined by real-time PCR. The expression of IL-1β and IL-17 was measured by immunohistochemistry and the expression of IL-17 in the kidney was measured by ELISA.RESULTS: Compared with control group, the expression of IL-1β, TGF-β1, IL-17 and LDLr, and cholesterol content in the kidney and the GSI were all significantly increased in ADR group (P<0.05). Compared with ADR group, 24 h urinary protein, serum creatinine, the expression of IL-1β, IL-17 and LDLr, and cholesterol content in the kidney were all significantly increased in ADR+BSA group (P<0.05). Treatment with SIMV significantly decreased the expression of IL-1β, TGF-β1, IL-17 and LDLr. The accumulation of cholesterol in the kidney and the GSI were also decreased (all P<0.05).CONCLUSION: Inflammation aggravates the lipid deposition and glomerular sclerosis by increasing the expression of LDLr in ADR mice. Albumin overload further accelerates the progressive kidney damage by regulating the expression of IL-1β, TGF-β1 and IL-17, which promotes the increase in LDLr. The beneficial effect of SIMV might be mediated by its anti-inflammatory effect. 相似文献
105.
本文对绿色营销的概念以及砀山梨绿色营销的现状进行了介绍,针对这一现状本文就如何加强砀山酥梨的绿色营销、促进砀山酥梨业持续健康发展提出了多方筹资用于开展绿色营销、制定绿色价格、塑造绿色形象、建立绿色信息平台、提高果农素质、普及绿色技术、加强绿色组织建设和品牌保护等对策. 相似文献
106.
详述了首次在国内午餐肉罐头生产中采用的微波解冻系统科技成果的实施情况、系统操作与安全控制特点以及运行条件。探讨微波解冻技术支持下,无亚硝酸钠午餐肉罐头的生产工艺原理及其品质卓越的原因。 相似文献
107.
108.
以试验模态分析为手段,研究IG-180A旋耕机整机振动模态特征,讨论了模态参数和频响函数分析用于旋耕机设计的问题,提出了旋耕机减重设计中应综合考虑强度可靠性和刚度可靠性的观点,并指出了补偿整体刚度的几种途径。 相似文献
109.
110.
Ⅱ优125是福建省南平市农科所用自育恢复系南恢125与Ⅱ-32A配组育成的杂交稻新组合,2003年通过福建省农作物品种审定委员会审定。2004年清流县引进经多点试验示范表现高产、稳产、适应性广,尤其在山区冷烂田表现较强的优势,增产效果明显等特点,深受农户喜爱,具有较大的推广前景 相似文献