Suction air traps vs. crops in monitoring Russian wheat aphid, Diuraphis noxia (Kurdj.) in Central Europe (Hom., Aphididae)

The Russian wheat aphid (=RWA), Diuraphis noxia (Kurdj.) is an exotic expansive aphid on the small grains determined in the Czech republic since 1993 (1995). Determination of RWA flight patterns by suction air traps (= SATs) between 1994-1998 indicated some banker, less important, or indicated RWA distribution areas. A comparison of the RWA monitoring by SATs and by crop samples in areas more or less distant from individual SAT locations manifested a high silmilarity of RWA evidence in its overall distribution and area-influenced population numbers. Owing to geographic area-dependent features of its life-cycle (holocycly) and host plant preferences, RWA seems to be most injurious to spring barley. In spite of the role of SATs in aphid forecasting, RWA samples are recommended to be taken also from the crops at the early ripening stage as the respective SAT evidence reflects only the emigration of the alate aphids originating from the decreasing populations. In general, RWA detection by SATs was either simultaneously or successively found to reflect the RWA presence in the more or less distant crops. The 30km perimeter of a SAT is agreed to be representative for mon-toring RWA populations in a sampled area.     

Direct detection of the asteroidal YORP effect

The Yarkovsky-O'Keefe-Radzievskii-Paddack (YORP) effect is believed to alter the spin states of small bodies in the solar system. However, evidence for the effect has so far been indirect. We report precise optical photometric observations of a small near-Earth asteroid, (54509) 2000 PH5, acquired over 4 years. We found that the asteroid has been continuously increasing its rotation rate omega over this period by domega/dt = 2.0 (+/-0.2) x 10(-4) degrees per day squared. We simulated the asteroid's close Earth approaches from 2001 to 2005, showing that gravitational torques cannot explain the observed spin rate increase. Dynamical simulations suggest that 2000 PH5 may reach a rotation period of approximately 20 seconds toward the end of its expected lifetime.     

Barley yield response to irrigation and potential profits in the Texas High Plains

Summary Irrigated winter barley (Hordeum vulgare L.) can be a profitable alternative to some low profit major crops in the Texas High Plains. A six-year evaluation of yield response related to total spring irrigation water, applied by surface methods (furrow), and seasonal precipitation resulted in a multivariate function explaining 74% of the yield variation. Predicted yields varied from a low of 3.69 Mg ha^–1 to a maximum 6.18 Mg ha^–1 with 0 and 389 mm, respectively, based on average monthly precipitation quantities. Precipitation is skewed to less than average in th semi-arid Texas High Plains. Using modal precipitation amounts of 40% of average precipitation, yield estimates were reduced to 2.29 Mg ha^–1 with zero spring irrigation and to 5.63 Mg ha^–1 at the peak with 450 mm. A second multivariate yield response function related to alternative timings of single and multiple spring irrigations explained 76% of the variation in yields. Among all combinations of 1, 2, 3, and 4 spring irrigations, irrigation water-use efficiency was estimated to be highest with one application at the boot stage of development. All other single and combinations of multiple irrigations resulted in lower water-use efficiencies. A comparison of enterprise budgets of four irrigation timing alternatives and levels of application indicated highest profit over variable costs, $ 287 ha^–1, was attained by applying a total of 307 mm in three spring applications at the boot, head, and milk stages. A lower level of 217 mm applied at boot and milk stages was $ 12 ha^–1 less profitable and a higher level of 425 mm was $ 24 ha^–1 less profitable. When fixed costs of irrigation facilities, land, and machinery were considered, returns to management and risk were highest, $ 101 ha^–1, with 217 mm. Using 40% of average precipitation, profits were reduced $ 65 ha^–1 with 217 mm and $ 69 ha^–1 with 307 mm spring irrigation levels.Respectively, agricultural economist, research scientist, and research associate, Texas Agr. Exp. Station, Amarillo, Texas; Emeritus Extension agronomist, Texas Agr. Ext. Service, Amarillo, Texas; agricultural engineer, Texas Agr. Exp. Station, Amarillo, Texas     

Blue aleurone trait diagnoses developmental origin of three pistils in a floret in common wheat

The common ‘three‐pistil’ (TP) wheat mutation line expresses TPs in a floret normally containing TPs forming three grains set close back‐to‐back. The developmental origin of the TP trait in common wheat had been diagnosed non‐destructively using the blue aleurone trait. The aleurone colour of F₂ seeds grown in F₁ plants of cross TP/UC66049 was evaluated. Due to xenia, the hue of blue grain colour depended on dose of the Ba1 gene for blue aleurone in the triploid endosperm. The TP trait produced four types of segregation in three‐seed clusters: (i) white grain only, (ii) two white grains and one blue, (iii) one white grain and two blue, and (iv) three blue grains only. The observed frequency of blue–white seed within clusters followed the binominal distribution ₃C_r (0.75)^r·(0.25)^3–r, where r is the number of colour variants in three‐seed clusters (r = 0–3). Intrafloret segregation of seed colour and F₂ segregation derived from aleurone colour of F₃ seeds indicated an independent origin of the TP trait.     

Distribution of race-specific resistance against <Emphasis Type="Italic">Bremia lactucae</Emphasis> in natural populations of <Emphasis Type="Italic">Lactuca serriola</Emphasis>

A metapopulation approach was applied to population studies of a common weed, Lactuca serriola (prickly lettuce). Seedlings grown from seed samples collected from 752 individual L. serriola plants in 50 populations occurring along an east-to-west transect across four European countries (Czech Republic, Germany, Netherlands and United Kingdom) were screened for resistance to 10 common races of Bremia lactucae. Based on the recorded reaction patterns, host individuals were characterized into specific resistance (R-) phenotypes. Diversity of R-phenotypes, their variation and distribution among and within European populations, was evaluated at different spatial scales, i.e. from a metapopulation involving the entire European study area to individual plants occurring in local populations. Generally, European populations of L. serriola have been shown to be highly susceptible to B. lactucae. However, large variation in L. serriola resistance was found both among and within individual countries. There was a clear gradient of increasing uniformity of race-specificity moving from central to western Europe, as well as a slight decrease in the diversity of R-phenotypes. Populations in the United Kingdom were the most divergent in terms of resistance structure from other geographic regions, and also were the most homogeneous, most likely a consequence of the relatively greater degree of spatial isolation from other regions. Metapopulation, inter- and intra-population variation in host resistance is discussed from the viewpoint of occurrence of race-specific interactions in this wild plant pathosystem.     

Manganese Biogeochemistry in a Central Czech Republic Catchment

Mn biogeochemistry was studied from 1994 to 2003 in a small forested catchment in the central Czech Republic using the watershed mass balance approach together with measurements of internal stores and fluxes. Mn inputs in bulk deposition were relatively constant during a period of sharply decreasing acidic deposition, suggesting that the Mn source was terrestrial, and not from fossil fuel combustion. Mn inputs in bulk deposition and Mn supplied by weathering each averaged 13 mg m^?2 year^?1 (26 mg m^?2 year^?1 total input), whereas Mn export in streamwater and groundwater averaged 43 mg m^?2 year^?1. Thus an additional Mn source is needed to account for 17 mg m^?2 year^?1. Internal fluxes and pools of Mn were significantly greater than annual inputs and outputs. Throughfall Mn flux was 70 mg m^?2 year^?1, litterfall Mn flux was 103 mg m^?2 year^?1, and Mn net uptake by vegetation was 62 mg m^?2 year^?1. Large pools of labile or potentially labile Mn were present in biomass and surficial soil horizons. Small leakages from these large pools likely supply the additional Mn needed to close the watershed mass balance. This leakage may reflect an adjustment of the ecosystem to recent changes in atmospheric acidity.     

Invertebrate grazing determines enzyme production by basidiomycete fungi

Extracellular enzymes produced by heterotrophic microorganisms in the soil are responsible for the decomposition of organic compounds. Basidiomycete fungi are the primary decomposer agents in temperate wooded ecosystems and contribute extensively to extracellular enzyme activity and nutrient mineralisation within soils. Growth and development of basidiomycete mycelia is influenced by soil-dwelling invertebrate grazers with potential implications for fungal activity and ecosystem functioning. The impacts of four invertebrate taxa belonging to Isopoda, Myriapoda, Collembola and Nematoda on the production of eight hydrolytic enzymes by four saprotrophic basidiomycetes (Phanerochaete velutina, Resinicium bicolor and two strains of Hypholoma fasciculare) were compared in a factorial microcosm study. Grazing generally increased enzyme production but invertebrates had species-specific impacts on enzyme activity. The magnitude of grazing influenced enzyme activity; macrofauna (woodlice and millipedes) induced the greatest responses. Enzymatic responses varied markedly between fungi. Grazing enhanced enzyme activity in the exploitative mycelial networks of P. velutina and H. fasciculare, while the opposite effects were observed in the explorative R. bicolor networks. The impacts of soil fauna on nutrient mineralisation depend on fungal community composition. β-glucosidase, cellobiohydrolase, N-acetylglucosaminidase, acid phosphatase and phosphodiesterase activities were affected most frequently by grazing and invertebrate activity, and thus had direct consequences for carbon, nitrogen and phosphorous cycling. The results indicate that invertebrate diversity and community composition may influence the spatial distribution and activity of extracellular enzymes with direct implications for nutrient mineralisation and trunover in woodland soils.     

Microsatellite DNA Analysis of Wild Hops, Humulus lupulus L.

To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci. There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster. Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America.     

Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED

Fungi are important in soils as both decomposers and plant symbionts, and an understanding of the composition of their complex communities is thus indispensable to answer a variety of ecological questions. 454 Pyrosequencing is currently the method of choice for the in-depth analysis of fungal communities. However, the interpretation of its results is complicated by differences in data analysis approaches that make inter-study comparisons difficult. The pyrosequencing studies published so far have also used variable molecular targets in fungal rDNA. Although the ITS region and, in particular, ITS1 appear to be the most frequent sequencing targets, the use of various primers with different coverages of fungal groups remains a serious problem. Sequence length limits also vary widely across studies, and in many studies, length differences may negatively affect sequence similarity clustering or identification. Unfortunately, many studies neglect the need to correct for method-dependent errors, such as pyrosequencing noise or chimeric sequences. Even when performed, error rates in sequences may be high, and consensus sequences created by sequence clustering therefore better represent operational taxonomic units. We recommend a data analysis workflow that includes sequence denoising, chimera removal, sequence trimming before clustering and random resampling before calculating diversity parameters. The newly developed free pipeline (SEED) introduced here can be used to perform all the required analytical steps. The improvement and unification of data analysis procedures should make future studies both more reliable and comparable and allow meta-studies to be performed to provide more general views on fungal diversity, biogeography or ecology.     

Kinematic Viscosity and Shear Stress of Used Engine Oil

The goal of this paper describes kinematic viscosity and shear stress of two used engine oils, which have been taken from two different passenger cars. Kinematic viscosity and shear stress are two of the most important physical behaviours of fluids, especially lubricating fluids. In this paper the authors have focused on engine oil. Knowledge of these properties of engine oil is very important due to its lifetime. The experiments have been done using digital rotary rheometer Anton Paar DV-3 P with use of TR8 spindle and special adapter for a small amount of sample （20 mL）. Two different engine oils have been observed--first from passenger car Renault Scenic with petrol engine （engine capacity 1.6 dm3） and the second from passenger car Skoda Roomster with diesel engine （engine capacity 1.4 dm3）. Castrol Magnatec 10W-40 engine oil has been taken from Renault car and Shell Helix Ultra Extra 5W-30 engine oil has been taken from ~koda car. Service interval of change oil has been set to 15,000 km and samples of used engine oils have been taken after 1,500 km. Only first samples of used engine oils have been taken after raid of 20 km. All samples of used engine oils have been compared with new （unused） engine oils same specification. The measured values of kinematic viscosity and shear stress have been modeled using linear function. The coefficients of correlation R have been achieved high values （0.88-0.96）. The obtained models can be used to prediction of engine oil flow behaviour.