全文获取类型
收费全文 | 2178篇 |
免费 | 113篇 |
国内免费 | 1篇 |
专业分类
林业 | 109篇 |
农学 | 132篇 |
基础科学 | 3篇 |
372篇 | |
综合类 | 158篇 |
农作物 | 274篇 |
水产渔业 | 186篇 |
畜牧兽医 | 898篇 |
园艺 | 34篇 |
植物保护 | 126篇 |
出版年
2023年 | 14篇 |
2022年 | 30篇 |
2021年 | 52篇 |
2020年 | 34篇 |
2019年 | 40篇 |
2018年 | 53篇 |
2017年 | 68篇 |
2016年 | 62篇 |
2015年 | 65篇 |
2014年 | 93篇 |
2013年 | 131篇 |
2012年 | 162篇 |
2011年 | 171篇 |
2010年 | 112篇 |
2009年 | 116篇 |
2008年 | 142篇 |
2007年 | 140篇 |
2006年 | 153篇 |
2005年 | 85篇 |
2004年 | 85篇 |
2003年 | 79篇 |
2002年 | 77篇 |
2001年 | 68篇 |
2000年 | 40篇 |
1999年 | 28篇 |
1998年 | 26篇 |
1997年 | 5篇 |
1996年 | 9篇 |
1995年 | 9篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 11篇 |
1991年 | 6篇 |
1990年 | 15篇 |
1989年 | 16篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1985年 | 6篇 |
1981年 | 3篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 7篇 |
1973年 | 10篇 |
1971年 | 3篇 |
1970年 | 2篇 |
1968年 | 2篇 |
1966年 | 4篇 |
1949年 | 2篇 |
1929年 | 2篇 |
排序方式: 共有2292条查询结果,搜索用时 328 毫秒
991.
Seung-Hyeok Seok Min-Won Baek Hui-Young Lee Dong-Jae Kim Yi-Rang Na Kyoung-Jin Noh Sung-Hoon Park Hyun-Kyoung Lee Byoung-Hee Lee Jae-Hak Park 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):351-357
Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhRE-EGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3'',4,4'',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies. 相似文献
992.
Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress
Mi Na Lee Sang Hun Lee Min Young Lee Yun Hee Kim Jae Hong Park Jung Min Ryu Seung Pil Yun Yu Jin Lee Mi Ok Kim Kwangsung Park Ho Jae Han 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(3):247-256
Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5α-reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure androgenic steroid. This study was conducted to examine the effect of DHT (10-7 M) on H2O2 (10-3 M) -induced injuries in mouse embryonic stem (ES) cells. H2O2 induced ROS generation and increased lipid peroxide formation and DNA fragmentation. These effects of H2O2 were inhibited by pretreatment with DHT. H2O2 also increased the phosphorylation of p38 MAPK, SAPK/JNK and nuclear factor kappa B (NF-κB), but DHT blocked these effects. Moreover, H2O2 decreased DNA synthesis and the levels of cell cycle regulatory proteins [cyclin D1, cyclin E, cyclin-dependent kinase (CDK) 2, and CDK 4]. These effects of H2O2 were inhibited by pretreatment with DHT. In conclusion, DHT may partially prevent H2O2-induced cell injury through inhibition of ROS and ROS-induced activation of p38 MAPK, SAPK/JNK and NF-κB in mouse ES cells. 相似文献
993.
Jeongtae Kim Changjong Moon Heechul Kim Jinwoo Jeong Juyeon Lee Jihoon Kim Jin Won Hyun Jae Woo Park Mi Yeon Moon Nam Ho Lee Sung Ho Kim Youngheun Jee Taekyun Shin 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(3):281-284
The radioprotective activity of extracts from the red seaweed Callophyllis (C.) japonica was investigated in mice that underwent whole-body exposure to gamma radiation. A methanol extract of C. japonica and its fractions [hexane, ethyl acetate (EtOAc), butanol and the remaining H2O] were used. Each fraction (100 mg/kg body weight) was administered intraperitoneally (i.p.) 2 times into the BALB/c mice, once at 1 and once at 24 h before exposure to 9 Gray (Gy) of gamma radiation. Pre-irradiation administration of the hexane and EtOAc fractions saved the mice, with their survival rates being greater than 80% at 30 days post-irradiation; the mice that were pretreated with the other fractions showed survival rates lower than 20% over the same time period. To examine the effect of each C. japonica fraction on the survival of intestinal and bone marrow stem cells, the number of intestinal crypts and bone marrow cells in the gamma-irradiated mice were examined. Pre-treatment of mice (i.p., 100 mg/kg body weight at 1 and 24 h before irradiation) with the hexane or EtOAc fraction prior to 6-Gy irradiation significantly protected the number of jejunal crypts and bone marrow cells at 9 days after irradiation. These findings suggest that certain extracts from C. japonica, when they are administered prior to irradiation, play an important role in the survival of irradiated mice, and this is possibly due to the extracts protecting the hematopoietic cells and intestinal stem cells against gamma irradiation. 相似文献
994.
Sun Hee Do Da-Hee Jeong Jae-Yong Chung Jin-Kyu Park Hai-Jie Yang Dong-Wei Yuan Kyu-Shik Jeong 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):425-427
Histopathological findings of eosinophilic myositis in the carcass of a slaughtered Korean native cow are presented. Lesions contained massive fibrous septae with vacuolar changes in some lesions, and the hypercontraction and rupturing of muscle bundles, with replacement by eosinophils. Necrosis and severe eosinophil infiltration were observed. Sarcoplasmic fragmentation and atrophy developed. Typical of granuloma, calcified myofibers were focally surrounded by macrophages and numerous inflammatory cells, and multinucleated giant cell formation was evident. 相似文献
995.
Yoo J Chang HH Bae YH Seong CN Choe NH Lillehoj HS Park JH Min W 《Veterinary immunology and immunopathology》2008,121(3-4):359-363
Chicken interleukin-17 (chIL-17) gene was previously characterized through cloning from a chicken intestinal expressed sequence tag (EST) cDNA library. To further investigate the biological properties of chIL-17, six monoclonal antibodies (mAbs) against a bacterially expressed chIL-17 recombinant protein were produced and their binding specificities characterized. Antibodies which were initially selected on the basis of their specific binding reactivity with recombinant chIL-17 in ELISA were further characterized by Western blot analysis. Monoclonal antibodies specific for chIL-17 identified 20 and 21kDa protein bands in the culture supernatant and cell lysate of CU205 cells. These mAbs also recognized specific bands for chIL-17 in the cell lysate from conconavalin A (Con A)-activated, but not from normal splenic lymphocytes. Furthermore, these mAbs detected a 16kDa protein in the lysate of CU205 cells treated with tunicamycin and stained an intracellular protein in CU205 cells in flow cytometric analysis. Together, these results indicate that these new mAbs are specific for chIL-17 and will be a useful tool for structural and immunological studies of IL-17 in poultry. 相似文献
996.
Park SJ Oh EH Park SI Kim HH Jeong YJ Lim GK Hyun BH Cho KO 《Veterinary microbiology》2008,126(4):364-371
The prevalence of the bovine torovirus (BToV) and its genetic characterization have been reported in North America, Europe and Japan. Therefore, this study examined the prevalence and genetic diversity of the BToV in a total of 645 diarrheic fecal samples from 629 Korean native beef calf herds using RT-PCR and nested PCR with the primer pairs specific to a part of the BToV membrane (M) gene. Overall, 19 (2.9%) out of 645 diarrheic samples from 19 herds (6.9%) tested positive for BToVs by either RT-PCR or nested PCR. A comparison of the nucleotide (nt) and amino acid (aa) sequences of a part of the BToV M gene (409bp) among the BToVs showed the Korean BToVs to have comparatively higher sequence homology to the Japanese and Dutch BToVs than to the American and Italian BToVs. Generally, the Korean BToV strains clustered with the Japanese and Dutch BToV strains. However, the American and Italian BToV strains clustered on a separate major branch, suggesting that these are more distantly related to other known BToV strains. These results suggest that the BToV infections are sporadic in diarrheic calves in South Korea, and the Korean BToV strains are more closely related to the Japanese and Dutch BToVs than to the American and Italian BToVs. 相似文献
997.
Dexamethasone induces apoptosis in proliferative canine tendon cells and chondrocytes 总被引:3,自引:0,他引:3
M A Hossain J Park S H Choi G Kim 《Veterinary and comparative orthopaedics and traumatology》2008,21(4):337-342
Dexamethasone (Dexa) has been commonly used in humans and domestic animals, particularly in the treatment of tendon injuries and cartilage degeneration. However, it is often associated with tendon rupture and impaired tendon and cartilage healing. In the present study, we investigated Dexa's in vitro effects on the growth of cell proliferation and the induction of apoptosis in canine Achilles tendon cells and chondrocytes. Cell proliferation after treatment with Dexa for two to six days was quantified by a 2,3-bis{2-methoxy-4-nitro-5-sulfophenyl}-2H-tetrazolium-5-carboxyanilide inner salt assay (XTT). The results showed that Dexa could inhibit the proliferation of tendon cells and chondrocytes at increasing concentrations (0.1-50 microg/ml) compared with untreated cells. Cell apoptosis was induced by Dexa, as evidenced by the typical nuclear apoptosis using Hoechst 33258 staining. Dexa increased the apoptosis of canine tendon cells and chondrocytes in a time-dependent manner. In canine tendon cells and chondrocytes that were treated with 25 and 50 microg/ml concentration of Dexa, the number of condensed apoptotic nuclei was significantly increased. In addition, culturing with Dexa and the glucocorticoid receptor blocker, mifepristone, significantly arrested apoptosis of tendon cells and chondrocytes. Based on our in vitro data, we hypothesized that in vivo treatment with glucocorticoids may diminish the proliferation of tendon and cartilage cells by increasing apoptosis and suppressing the proliferation. Our findings suggest that Dexa could be used with caution in dogs with articular or tendon problems. 相似文献
998.
Hwang IK Yoo KY Li H Park OK Lee CH Choi JH Kwon DY Won MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):1005-1010
In this study, we investigated changes in glutamic acid decarboxylase 67 (GAD67) immunoreactivity and its protein levels in the gerbil somatosensory cortex after ischemia/reperfusion. GAD67 immunoreactivity was significantly increased in layers III and V of the somatosensory cortex 12 hr after ischemia/reperfusion. Thereafter, GAD67 immunoreactivity was decreased with time after ischemia/reperfusion. GAD67 immunoreactivity in the somatosensory cortex 4 days after ischemia/reperfusion was similar to that in the sham-operated group. In addition, GAD67 protein levels were also significantly increased 12 hr after transient forebrain ischemia. These results suggest that the transient increase of GAD67 immunoreactivity in layers III and V may be associated with responses to transient ischemia-induced neuronal damage. 相似文献
999.
Hyun-Kyu Park Woori Jo Hyun-Ji Choi Sungwoong Jang Jae-Eun Ryu Hyo-Ju Lee Hyojin Lee Hyejin Kim Eun-Sil Yu Woo-Chan Son 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):45-51
Drug-induced liver injury (DILI) is a significant threat to patient health and a major concern during drug development. Recently, multiple circulating microRNAs (miRNAs) have been reported to be potential biomarkers for DILI. To adapt and validate miRNAs for clinical use, we investigated the time-course changes in miR-122 expression levels in an acetaminophen-induced liver injury model in rats. In addition, miR-155 and miR-21 were evaluated as makers of inflammation and regeneration, respectively, to characterize liver status. Our results revealed that miR-122 is an early and sensitive biomarker of hepatocellular injury at a stage when alanine transaminase, aspartate transaminase, and total bilirubin were not detectable. However, no significant differences in the expression levels of other miRNAs (miR-155 and -21) were observed between treatment and vehicle groups. Collectively, these time-course changes in the expression levels of miRNAs may be useful as markers for clinical decision-making, in the diagnosis and treatment of DILI. 相似文献
1000.
Ahmed glaucoma valve implantation with Ologen® Collagen Matrix for the surgical treatment of feline glaucoma 下载免费PDF全文
Kang‐hyo Park Joon‐Young Kim Seoung‐woo Choo Hun‐young Yoon Soon‐wuk Jeong 《Veterinary ophthalmology》2018,21(1):96-100
An Ahmed valve implantation with an Ologen® Collagen Matrix (Ologen® CM, Aeon Astron, Leiden, the Netherlands) was performed for the treatment of uncontrolled glaucoma in a cat. This cat was a 5‐year‐old castrated Russian Blue male with a 12‐week history of conjunctival hyperemia and mydriasis of the left eye. During the ophthalmic examination, the intraocular pressure (IOP) oculus sinister (OS) was 52 mmHg, and a narrow iridocorneal angle (ICA) was detected by gonioscopy. Medical treatment with Cosopt® (2% dorzolamide and 0.5% timolol) failed to decrease the IOP. The left eye still had vision, and an Ahmed valve implantation was performed. During the gonioimplantation, Ologen® CM was used to inhibit scar formation around the valve. Following the operation, the IOP was stable at an approximate average of 15 mmHg during the 7‐month follow‐up period, and vision in the left eye was retained without medication. An adequate subconjunctival filtering bleb was formed after 140 days. This is the first case report in which an Ahmed valve gonioimplant with an Ologen® CM has been used for the surgical treatment of glaucoma in a cat. 相似文献