Identification of serum differential proteins in colorectal adenoma and early malignantly transformed adenoma by proteomics

AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma.     

Effects of apolipoprotein (a) on vascular smooth muscle cells proliferation and cell signal transduction pathway

AIM: To investigate the effect and the mechanism of apolipoprotein (a) ［apo (a)］ on proliferation of vascular smooth muscle cells (VSMCs). METHODS: All VSMCs used in experiments were serial subcultured from primary cells and were identified by immunohistochemistry staining of α-actin. Cell growth assay was observed as cell counting and MTT assay. Western blotting was also employed to detect the related mechanism. RESULTS: All cells used in experiments were confirmed as VSMCs. Although apo (a) enhanced VSMCs proliferation, this effect was attenuated by anti-integrin αⅤβ3, LM609. Use these reagents alone had no effect on VSMCs growth. The results of Western blotting demonstrated that focal adhesion kinase (FAK) was activated by apo (a) and the expression of total or phosphorylated transforming growth factor β1 (TGF-β1) was also decreased. However, these effects described above were all blocked by LM609. CONCLUSION: Apolipoprotein (a) enhances VSMCs proliferation and this effect is mediated by integrin αⅤβ3, which activates FAK and attenuates TGF-β1 and phospho-TGF-β1 expression.     

不同基质及生根剂浓度对五种园林植物扦插生根的影响

以5种园林植物为材料,进行不同基质与不同生根剂浓度对植物扦插生根的比较研究.结果表明:月季、大叶黄杨最适宜的基质是河沙,小叶女贞、八角金盘最适宜的基质是珍珠岩,鹅掌柴最适宜的基质是混合基质.月季、大叶黄杨最合适的生根剂浓度为3 000 mg/kg,小叶女贞最合适的生根剂浓度为5000mg/kg,八角金盘、鹅掌柴最合适的生根剂浓度为1000mg/kg.     

中小果型无籽西瓜新品种博帅的选育

博帅系湖南博达隆科技发展有限公司以9048为母本、M-2为父本杂交育成的中小果型黄瓤无籽西瓜新品种。该品种全生育期90d左右,雌花开放至果实成熟约需27d。植株生长势中强,抗病抗逆性强。果实短椭圆形,果形指数1.2。果皮浅绿底覆绿色齿条带,厚度0.9cm,硬韧,耐贮运。瓤色鲜黄,质脆爽口,纤维少,无籽性能好;中心可溶性固形物12%～13%,边部9%～9.5%。单果质量4kg左右,667m2产量2700kg左右。适应性广,全国各地均可栽培。2009年3月通过湖南省农作物品种审定委员会审定,审定编号:湘审西瓜2009002。     

引进美系大白猪的适应性研究与生产性能测定

为充分了解新美系大白猪的生产性能,自主培育能满足不同市场需求、生产性能好、适应性强的大白猪父本新品系,我们对引进的美系大白猪进行了适应性研究与生产性能测定.结果表明,新美系大白猪在主要经济性状上与国内原有大白猪相比具有一定优势,是一个良好的育种素材.但同时也表现出一定的缺点,在使用过程中应引起用户注意,不要盲目跟从.     

农田耕作措施对近地表风沙运动的影响

我国北方传统耕作模式的犁耕、耙磨及磨平整地措施对农田近地表风沙活动影响显著。通过对春季半干旱沙区农田风沙活动的观测,本文对犁耕、耙磨磨平两种处理下近地表0~60 cm内输沙率和风沙流结构随田块长度的变化规律进行了研究。结果表明,耙磨磨平处理破坏了犁耕在农田地表形成的非可蚀性土块结构,使近地表0~60 cm内输沙率增大,在强沙尘事件中增加幅度可达数10倍。犁耕地表上输沙率随田块长度的增加呈指数关系递增,而耙磨磨平后风沙流发育的饱和路径长度减小,在强沙尘事件中近地表输沙率随田块长度的增加而递减。此外,上风向处犁耕地表低层输沙比例(Q0-10/Q0-60)小于耙磨磨平地表,且随田块长度的增加逐渐增大,而耙磨磨平后风沙流结构随田块长度的变化不大。     

中国城镇化过程中村庄土地整理潜力估算

该文在理论分析的基础上，创建了一个定量的数学模型来估算村庄土地整理净增加的潜力。估算结果表明，全国平均整理系数2010年为1.42～2.42、2030年为2.27～3.27，分别可净增加耕地面积173.81～296.58万hm²、279.10～401.86万hm²。通过对不同时间和不同生态区的整理系数比较，村庄土地整理潜力具有明显的时空分布规律。在时序上，随着社会经济的发展和城镇化水平的提高，我国农村人口会逐渐减少，整理潜力逐渐增大；在空间上，东部和中部村庄土地     

基于遗传算法的遥感数字图像镶嵌研究

遥感数字图像的镶嵌处理是遥感图像处理的基本工作之一，为此介绍了一种基于遗传算法确定图像重叠区和调整图像色度，以辅助完成遥感图像的镶嵌工作的新方法。     

桂西北喀斯特人为干扰区植被自然恢复与土壤养分变化

为促进桂西北喀斯特退化生态系统的恢复与重建,采用全面调查和样方调查方法,以自然保护区的顶级群落为对照,研究了桂西北喀斯特人为干扰区自然恢复22a后植被的演替规律与土壤养分变化。结果表明,干扰区物种丧失严重,种类仅有自然保护区的26.6%,随着群落由草丛→草灌丛→灌丛→藤刺灌丛→乔灌丛→顶级群落的顺向演替和发展,群落的高度、生物量和物种多样性、土壤有机质、养分、阳离子交换量和硅、铁、铝、钛等矿质全量逐步增加,钙、镁全量显著减少,pH值降低,土壤质量随着植被的恢复呈波折性提高。     

有机磷除草剂草甘磷(GPS)对作物细胞遗传学毒性效应的研究

利用有机磷除草剂草甘磷(Glyphosate,GPS),在不同浓度、不同处理时间下,对作物(黑麦、大麦)根尖分生组织细胞有丝分裂的影响进行了研究。结果表明,在浓度为50mg·L-1、处理时间为4h时,能引起细胞异常有丝分裂如多极、染色体桥、间期微核形成、细胞有丝分裂指数降低。草甘磷对黑麦、大麦细胞异常有丝分裂的临界浓度和临界时间分别是50mg·L-1和4h。