应用阻断酶联免疫吸附试验检测牛白血病病毒抗体

多年来,都用琼脂凝胶免疫扩散(AGID)试验检测牛白血病病毒(BLV)抗体。而此疫病的普查和根除需要检测大量的样品,酶联免疫吸附试验就可满足这一要求,间接法是先将提纯的抗原包被到固相载体,再加试验血清和特异性IgG结合物,阻断法是利用试验血清中的抗体干扰或阻断标准抗原-抗体     

柑桔苗木的繁殖

建立一个成功的柑桔苗木繁殖计划以及由此形成的高产柑桔业所面临的问题是要生产无病毒的园艺性状优良的材料。使用无病毒苗木并通过良好的栽培管理和密植栽培方式就能最大地发挥它们的园艺学潜力。没有诸如鳞皮病、裂皮病和剥皮病这些使树衰弱的病害将使柑桔产量增加50%以上。开发无     

Strategic Ideas of Greenway Construction in Ecological Zhejiang Province

INTRODUCTIONThe Zhejiang Provincial Committee of the ChineseCommunist Party and the Zhejiang ProvincialGovernment, followed the natural law and the economicsociety law of development, has laid down the strategicdecision of "to make the ecology province constructionas the carrier, makes afforested Zhejiang" strategicdecision from 2002. From now on, within 20 years,Zhejiang Province will be a sustainable developmentprovince with developedeconomy, exquisite ecologicalenvironment, harmoni…     

放牧与圈养育肥牛皮下脂肪组织中脂肪酸的代谢和沉积

本试验旨在评价放牧和圈养对安格斯杂交阉牛脂肪酸代谢的影响（n=24）。在试验的0d、28d、84d和140d收集瘤胃液、血清和脂肪组织样本检测。每隔14d收集牧草和日粮养分样本评定营养价值和脂肪酸组成。圈养育肥牛日粮由玉米青贮、破碎玉米、豆粕、维生素和矿物质构成。放牧阉牛的放牧顺序依次为黑小麦／一年生黑麦草、苜蓿／鸭茅和喜冷牧草／豆科牧草。圈养育肥牛日粮脂肪酸的57％是油酸，2％为亚麻酸。     

德国花兔与青紫蓝兔母性行为及繁殖力的比较

母兔的良好母性行为可以提高仔兔的生长速度、成活率及增强仔兔体质,也影响到断奶适宜日龄,从而影响后期生长育肥性能。本试验以两个品种——德国花兔及青紫蓝兔(20只母兔)为研究对象,对母兔分娩前后不同行为进行观察测定产仔数、泌乳力、断奶窝重、成活率等指标并比较分析了这两个品种兔的母性强度,并得出如下结果:两品种母兔的母性行为及繁殖力无明显差异。     

热力学键合研究——蛋白聚合/蛋白活性对蛋白质吸着水蒸汽作用的实际影响

一、导言食品水分吸着等温线具有非常实用的重要意义,并一直是各种热力学和数学研究解决的课题.一般情况下,在食品蛋白质和其他系统里可以观察到S形或Ⅱ巨型水分吸着等温线.相对水蒸汽压力或“水分活性”(α_w=P/P_0),和水湿含量M之间的关系往往用方程式来表示,这些方程式可以用来进行数据的线性回归分析.一个显著的例外就是5参数型/水蒸汽吸着等温线的非线性回归分析,这就是 D＇Arcy和Wait(1970,1981)就食品蛋白和其它系统引入的.D＇Arcy和Watt(1970,1981)的模型包括把单个水分子结合为两个不同位置的类型:强和弱.此外,水分活性α_w高时,该模型假设多分子层水分的某一“偏差”结合到大分子表面.随着水湿含量由低到高的增加,这一等位结合模型进一步假设所有类型(包括多分子层类型)的结合区位置,同时被不同程度地占满.这样,根据这一模型,即使α_w处于很低的情况下多分子层水就会小量出现.D＇Arcy和Watt模型确实是一个等位结合型,有关论据在附录里给出,在该附录当中,D＇Arcy和Watt理论吸附等温线的一个完整求导是以水结合方程式给出的.其后模型里所包括的隐含假设和近似数也通过该求导公式在附录中给出.如前所述,由于多分子层假设,D＇Arcy和Watt模型在αw≥0.93高的情况下,不能很好适于实验吸着等温线     

Estimating Combining Ability of 4x-2x Crosses in Cultivated Potatoes

Progenies derived from crosses between Solarium tuberosum and 2n pollen-Producing diploid hybrids, exhibit obvious hybrid vigor. The 2n pollen-producing clone can act as a bridge in crossing S. tuberosum and S. andigena with S. phureja. Populations from 4x-2x crosses show more unifomity and less segregation compared with that of 4x-4x crosses. The parent-offspring correlation for the traits, starch content and tuber number, is significant at 0.01 level. The regrssion equations are Y (mp-F1)=1.0 1.2x and Y (mp - F1) = 5.3 0.8x, respectively. The 2n pollen-producing clones play an impotant role in increasing tuber stach content. Estimates of the combining ability for the main yield components indicate that additive effect prodominatcs for such trais as plot yield, tuber weight per plant and starch content, whereas both additive and non-additive effects lay equal stress on mean tuber weight and non-additive effect is important for tuber number. In general, non-additive effect appears to be important in     

Assessment of Negative Training on the Use of Cubicle Beds by Heifers

40 in-calf heifers were observed to examine the effect of timbers placing in passage area on their use of cubicle beds. Prior to the experiment preliminary observation was taken on these 40 heifers and they were divided into two even groups (cubicle user(or control) group versus cubicle refusal (or experimental) group by whether they properly used the cubicle beds or not. The timbers were placed in passage and feeding areas for the cubicle refusal group to force the animls to lie in the cubicle beds. The results of this experiment showed that feeding behaviour was unaffected by the presence of the timbers, but lying acitvity was about 8% higher for the experimental group, which led to a mean of almost 2 hours longer lying time over 24 h. After the timbers were removed, there was a significant decline in lying by the experimental group. The diurnal pattern of lying behaviour in cubicles indicated that the major effect on lying activity exerted by the presence of the timbers was seen during midnight to 10:00 a