Comparative Study on the Chemical Composition,Antioxidant Properties and Hypoglycaemic Activities of Two <Emphasis Type="Italic">Capsicum annuum</Emphasis> L. Cultivars (<Emphasis Type="Italic">Acuminatum</Emphasis> small and <Emphasis Type="Italic">Cerasiferum</Emphasis>)

The present study aimed to evaluate for the first time the phenols, flavonoids, carotenoids, capsaicin and dihydrocapsaicin content and the antioxidant and hypoglycemic properties of Capsicum annuum var. acuminatum small and C. annuum var. cerasiferum air-dried fruits. The ethanol extract of C. annuum var. acuminatum small, characterized by the major content of total poliphenols, flavonoids, carotenoids and capsaicinoids, showed the highest radical scavenging activity (IC₅₀ of 152.9 μg/ml). On the contrary, C. annuum var. cerasiferum showed a significant antioxidant activity evaluated by the β-carotene bleaching test (IC₅₀ of 3.1 μg/ml). The lipophilic fraction of both C. annuum var. acuminatum and C. annuum var. cerasiferum exhibited an interesting and selective inhibitory activity against α-amylase (IC₅₀ of 6.9 and 20.1 μg/ml, respectively).     

The dual crop coefficient approach to estimate and partitioning evapotranspiration of the winter wheat–summer maize crop sequence in North China Plain

An accurate estimation of crop evapotranspiration (ET _c) is very useful for appropriate water management; hence, an accurate and user-friendly model is needed to support related irrigation decisions. In this view, a study was developed aimed at estimating the ET _c of winter wheat–summer maize crop sequence in the North China through eddy covariance measurements, to calibrate and validate the SIMDualKc model, to estimate the basal crop coefficients (K _cb) for both crops and to partition ET _c into soil evaporation and crop transpiration. Two years of field experimentation of that crop sequence were used to calibrate and validate the SIMDualKc model and to derive K _cb using eddy covariance measurements. Various indicators have shown the goodness of fit of the model, with estimated values very close to the observed ones and estimate errors close to 0.5 mm d^?1. The initial, mid-season and end basal crop coefficients for wheat were 0.25, 1.15 and 0.30, respectively, and those for maize were 0.15, 1.15 and 0.45, thus close to those proposed in FAO56 guidelines. The soil evaporation represented near 80 % of ET _c for the initial stages of winter wheat and summer maize and decreased to only 5–6 % during the mid-season period. Evaporation during the full crop season averaged 28 % for winter wheat and 40 % for summer maize. The importance of wetting frequency and crop ground coverage in controlling soil evaporation was evidenced.     

Nitrogen Use Efficiency in Cacao Genotypes

Cacao (Theobroma cacao L) is mostly grown on soils with low natural fertility. On such soils nitrogen (N) is one of the most yield limiting nutrients for cacao. Information is lacking on N use efficiency in cacao. A greenhouse experiment was conducted to evaluate growth response and N use efficiency by two cacao genotypes. The genotypes used were TSH-565 and ICS-9 and N rates adapted were 0, 120, 240, 360, and 480 mg N /pot. In both genotypes, increasing levels of applied N improved growth (stem girth, dry weight of shoot and roots and shoot/root ratio), and concentration and uptake of N. Genotypes differed significantly for stem girth and ICS -9 produced greater stem girth compared with TSH-565. Nitrogen uptake had a linear relationship with root dry weight of the two genotypes. In both genotypes, increasing levels of applied N overall increased N-uptake efficiency (NEFF = N concentration in shoot x shoot/root), but decreased N-use efficiency by shoot and roots (NUE = g dry matter of shoot or root/mg N) and N-use efficiency of carbohydrate (NUEC = mg of total carbohydrates in shoot/mg of N in shoot). Both genotypes responded differently to applied N, despite the existence of close genetic relatedness between them. The method used here appears to be suitable method for identification of cacao genotypes that are efficient in uptake and utilization of N.     

ALUMINUM TOXICITY IN CORN PLANTS CULTIVATED WITH LOW AND HIGH IONIC STRENGTH NUTRIENT SOLUTIONS

The objective of the present study was to evaluate the phytotoxic effect of aluminum (Al) in corn plants in nutrient solution under high ionic strength (0.0094 uM) and low ionic strength (0.00001 uM). Regarding the low ionic strengths, nutrient solution was added daily until the end of the experimental period. The findings showed that the pH of the nutrient solution reduced with the increase in Al concentration, under both high and low ionic strengths solutions. Electrical conductivity of the nutrient solution increased with Al concentrations only in the solution with low ionic strength, demonstrating that the plant was more sensible to the element toxicity, thus reducing its growth and absorption of nutrients. The production of the total dry mass of corn was affected by the increase of Al concentration in the solution only under low ionic strengths, which reinforces the greater activity and absorption of the element under such condition.     

Horseradish (Armoracia rusticana), a neglected medical and condiment species with a relevant glucosinolate profile: a review

Armoracia rusticana (horseradish), a member of the Brassicaceae family, has been known since ancient times as a folk medicinal herb and as a plant of nutritional value and culinary interest. Currently horseradish is cultivated for its thick, fleshy and white roots which have a delicious intense pungency and for its tender leaves which are frequently used for salad mixed to other vegetables. The traditions to use horseradish plant for medicinal purpose are still applied in many countries. Horseradish is a rich source of a number of bioactive compounds such as glucosinolates (GLSs) and their breakdown products. Sinigrin is the dominant glucosinolate in both leaves and roots. Recent studies have shown that crude plant extracts have a complex profile of naturally occurring GLSs, with particular regard to sprouts. The increasing interest in these secondary metabolites, associated to the long and diffuse tradition of using horseradish in food preservation and as condiment in many parts of the world, is generating new applications of this plant in several agro-industrial and pharmaceutical sectors and is encouraging the use of its roots and leaves in functional food and medicine for human health. A bibliography review is discussed on ethnobotanical aspects and uses of this plant, as well as knowledge about its flavour compounds and GLS content and composition. This study summarizes also the updated information concerning the influence of the genotype and environment on GLS profile in horseradish.     

Impact of Sewage Sludge Compost Utilization on Chemical Properties of Olive Grove Soils

Field experiments were conducted for four years, between 1998 and 2002, in two olive grove soils of adult olive orchards (Olea europaea L. cv. Cornicabra) in a clay loam soil in Seseña (Toledo, Spain) and in a sandy loam soil in Aranjuez (Madrid, Spain). There were four treatments, sewage sludge compost (SSC), sewage sludge compost plus urea (SSC+U), urea (U) and control (C). Each treatment was replicated four times and two depths were studied (0-15 and 15-30 cm). Once a year, before spreading sewage sludge compost, soil samples were taken at depths of 0 to 15 cm and 15 to 30 cm. Organic matter, total Kjeldhal nitrogen, phosphorus availability, pH, and electric conductivity were measured. No differences were found between treatment on organic matter and electric conductivity after four years of application of sewage sludge compost to two olive grove soils. In relation to nitrogen content, sewage sludge compost, only in Seseña, produced higher nitrogen soil content than the traditional urea treatment. Sewage sludge compost applied on olive grove soils improved the Phosphorus availability for the olive tree. In Aranjuez, the use of sewage sludge compost increased the pH of the soil with respect to Urea and Control plots. In Seseña, the reverse effect was found.     

Evaluation of common bean genotypes for phosphorus use efficiency

A greenhouse experiment was conducted to evaluate phosphorus (P)‐use efficiency of 10 promising genotypes of common bean (Phaseoius vulgaris L.) with short and normal growth duration. The genotypes were grown on an Oxisol at 25 mg P kg^‐1 (low P) and 150 mg P kg^‐1 (high P) of soil. Shoot and root dry weight, root length, P concentration in the shoot, and P uptake in the shoot were significantly (P<0.01) affected by soil P concentration and genotype. However, P level did not effect root length and genotype had no effect on root dry weight. On the basis of P‐use efficiency (mg dry weight of shoot/mg P accumulated in the shoot) genotypes were classified as efficient and responsive (ER), efficient and nonresponsive (ENR), nonefficient and responsive (NER), and nonefficient and nonresponsive (NENR). From a practical point of view, genotypes which produce a lot of dry matter in a soil with a low P level, and respond well to added P are the most desirable because they are able to express their high yield potential in a wide range of P environments. Novo Jalo and Pérola genotypes fall into this group. Genotypes Irai, Jalo Precoce and L93300166 fall into the ENR group. Genotypes Carioca, Rosinha G‐2, and Xamengo were classified NER, whereas, genotypes L93300176 and Diamante Negro were classified as NENR. There were no differences between short and normal growth duration genotypes in P‐use efficiency.     

Application of the Weng’s ratio for the identification of Zn, Cu, and Pb contamination in soils and sediments

Purpose

This work explores the application of the use of Zn, Cu, and Pb relative contents as a new type of normalization method for geochemical properties of soils and sediments in an Atlantic Basin (Anllóns River, NW Spain). The method is based on the conservative behavior of these elements, which exhibit a certain concentration ratio that remains stable as long as there are no human disturbances.

Materials and methods

The average relative contents of Zn, Cu, and Pb were calculated by dividing the concentration of each metal in soils or sediments, in the <63-μm fraction, by the sum of Zn, Cu, and Pb, expressed as a percentage. The evaluation of the sum of the average relative concentrations of Zn, Cu, and Pb (Ri), together with three standard deviations for each element, namely, Ri ± 3Si, allows a hexagon to be constructed, represented in ternary diagrams of Zn:Cu:Pb. Following the method proposed by Weng et al. (Environ Geol 45:79–85, 2003), those samples falling outside the hexagon must be considered outliers.

Results and discussion

Results obtained confimed the conservative behavior between the relative contents of Zn, Cu, and Pb in surficial samples (soils, bed, and suspended sediments). Only sediment cores displayed nonconservative behavior, showing a marked Pb enrichment, with respect to the surficial samples. When Zn, Cu, and Pb relative contents were plotted in ternary diagrams, outliers were best classified when the hexagon was drawn with standard deviations of samples from the study area. The hexagon drawn with an international database of soils and sediments showed a poorer classification of outliers.

Conclusions

The results showed that total Zn, Cu, and Pb relative contents may be employed to investigate anthropogenic disturbances of these elements in soils and sediments of the Anllóns River Basin, thus corroborating that this type of normalization may be employed as a tool to assess outliers in a contaminated area.     

A multivariate approach for anomaly separation of potentially toxic trace elements in urban and peri-urban soils: an application in a southern Italy area

Purpose

Geogenic soil enrichment and anthropogenic pollution by potentially toxic trace elements (PTEs) are two processes acting together. Although it is often difficult, it is necessary to separate the two processes for risk assessment and understanding the environmental implications. The aim of this study was to analyse the soil concentrations of various PTEs in a southern Italy area in order to: (1) determine their different correlation structure to isolate sources of variation acting at different spatial scales and (2) to define potential anomalies based on the correlation structure.

Materials and methods

In the urban and peri-urban area of Cosenza-Rende, 149 topsoil samples were collected (0.10 m) and analysed for different elements by X-ray fluorescence spectrometry. Principal component analysis and factorial kriging analysis were used to map the spatial distribution of PTEs in topsoil and to identify the main factors influencing their spatial variability.

Results and discussion

Two groups of PTEs were identified: the first group included As, Pb and Zn; and the second one Al, Co, Cr, Fe, La, Nb, Ni, Ti and V. The first group was related to anthropogenic causes, while the second one was more related to parent rock composition. The regionalized factors at different scales of variability allowed to aggregate and summarize the joint variability in the PTEs and consider the probable causes of soil pollution.

Conclusions

The study allowed analysing and quantifying the sources (environmental or anthropogenic) of variation of PTEs acting at different spatial scale and defining the spatial anomalies based on the correlation structure associated at the different spatial scales.