Z-ligustilide isolated from Radix Angelicae sinensis ameliorates the memory impairment induced by scopolamine in mice

We investigated the effect of Z-ligustilide (LIG) on scopolamine-induced memory impairment in ICR mice. LIG (2.5–40 mg/kg) or tacrine (10 mg/kg) was orally administrated for 26 days. Behavior was examined in the Morris water maze and Y-maze after scopolamine administration (2 mg/kg, i.p.). The central acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) activities were assessed spectrophotometrically. LIG significantly improved spatial long-term memory and short-term memory impairment, inhibited AChE activity and increased ChAT activity. Moreover, LIG and tacrine showed the comparable efficacy in both neurobehavioral and cholinergic evaluation. These data suggest that LIG may alleviate memory deficits probably via enhancing cholinergic function.     

Partial replacement of fishmeal with Clostridium autoethanogenum single‐cell protein in the diet for juvenile black sea bream (Acanthopagrus schlegelii)

Clostridium autoethanogenum protein (CAP) is a single‐cell protein derived from ethanol production and may have potential to become a substitute for fishmeal in aquafeeds. A 70‐day feeding trial was conducted with black sea bream (Acanthopagrus schlegelii) (mean initial weight 6.03 ± 0.09 g) to evaluate partial replacement of fishmeal with CAP in diets. Seven isonitrogenous and isoenergetic diets were formulated with graded levels of CAP (0, 4.85, 9.70, 14.55, 19.40, 38.80 and 58.20%) to replace fishmeal. The inclusion of CAP at all dietary levels tested did not significantly affect the growth performance (p > .05). Fish fed the CAP58.20% diet showed a significantly lower feeding rate, with significantly higher protein efficiency ratio and feed efficiency ratio compared with fish fed the other diets (p < .05). No statistical differences were found in dorsal muscle and whole‐body compositions. Total superoxide dismutase in serum of fish fed CAP58.20% diet was significantly lower compared with that of the control. Malondialdehyde, catalase, total antioxidant capacity and digestive enzyme activities revealed no significant differences among dietary treatments. Phosphorus retention efficiency significantly increased, and phosphorus discharge showed a downward trend with increasing CAP inclusion levels. In conclusion, the results indicated that CAP is a safe and effective alternative protein source, which can replace fishmeal in the diet of black sea bream up to 58.20%, without adverse effects on growth performance, antioxidation and digestive enzyme activity. This study has shown the potential of converting industrial waste into a high protein feed ingredient for aquafeeds.     

狗头枣GSTU基因的克隆及其序列分析

为探索红枣GSTU类基因在红枣抗逆中的分子作用机制,本研究以狗头枣枣树叶片的DNA为模板,根据枣GSTU基因的序列(HM345954.1)设计引物,采用PCR方法获得GSTU基因序列,并利用生物信息学手段对其所对应蛋白的结构域、功能域、理化特性、跨膜区、二级结构及亚细胞定位等进行分析。结果表明:克隆到的DNA序列全长838 bp,具有2个外显子和1个内含子,内含子在292~460 bp碱基处,该序列与GenBank中枣的GSTU核苷酸序列和氨基酸序列的同源性分别为99.85%和100%,表明成功的克隆了狗头枣的GSTU基因。该序列编码222个氨基酸,分子量为25.268 kD,理论等电点(pI)为6.10;亲水性的平均数为-0.242,表明该蛋白是亲水性蛋白。二级结构主要以α-螺旋为主,其次是无规则卷曲,不存在信号肽,表明该蛋白可能为非分泌性蛋白,该蛋白可能位于内质网(膜)和质膜上。本研究获得的狗头枣GSTU基因在基因结构上含有1个内含子,在核酸序列及氨基酸序列上与GenBank中枣的GSTU基因同源性高,并对其理化性质、结构等进行了预测与分析,为进一步研究红枣抗逆分子机制提供理论基础,同时为红枣品种的选育提供理论支持。     

转基因抗虫保铃棉(Bollgard~ひ)检测方法研究

运用常规 PCR方法和实时荧光 PCR方法对转基因抗虫保铃棉外源基因 Ca MV 35 S启动子、NOS终止子、标记基因NPT II和目的基因 Cry IA(c)进行了检测。建立了快速、准确的抗虫保铃棉籽转基因成分的 PCR检测方法。     

不同超干方法与几种蔬菜种子储藏效应的研究

 采用硅胶干燥、真空冷冻干燥、低温低湿干燥以及加温干燥4 种方法将6 种蔬菜种子的含水量降到5 %以下, 在常温储存条件下保存10 年后进行的发芽试验表明其发芽率与10 年前比较没有明显变化, , 唯加温干燥的种子降低了萌发时抵抗逆境的活力, 种子生活力均未降低。     

早熟大粒优质葡萄新品种———早黑宝

 ‘早黑宝’是以‘瑰宝’ ב早玫瑰’的杂交种子用秋水仙素诱变育成的欧亚种四倍体葡萄新品种, 极早熟、果粒大、紫黑色、优质、丰产。     

大棚春大白菜播期和品种比较试验

春大白菜栽培对播种期要求非常严格 ,播种过早由于低温春化易引起先期抽薹使生产蒙受经济损失 ,播种太迟则在生长后期遇到高温导致包球不实或叶球腐烂。不同地区由于气候的差异应选择不同的播种期。为此 ,笔者进行了北京春大棚大白菜栽培的适宜播种期试验 ,以期为春大白菜生产提供技术指导。1　材料和方法1.1　大棚播期试验　以北京蔬菜研究中心新育成的春大白菜品种京春王为试材 ,1997年春季于温室采用营养钵播种育苗 ,设播期处理为 2月 19日、2 6日 ,3月 11日 ,育苗温度保持在 13℃以上 ,苗龄 1个月 ,分别于 3月 2 1日、2 8日 ,4月 10日定…     

大白菜新品种中熟5号的选育

自交不亲和系 2 3 5 8s是从大陆型的洛阳大包头中选育的 ;优良自交不亲和系H 1 3 1是从海洋型的本地优质笔尾白的自然杂交后代中分离出的。两个亲本经过严格的抗病性鉴定 ,亲和指数测定 ,配合力分析 ,配制成优良的一代杂种中熟 5号。该品种高抗霜霉病、软腐病、黑斑病、菌核病和病毒病 ,比当地主栽品种增产 10 %以上 ,现已在福建省及其它南方地区大面积推广。     

高山夏秋黄瓜配套技术研究初报

通过对高山夏秋黄瓜适宜种植地的选择,品种的筛选,播种期,密植程度及病虫防治等技术的试验研究,提出一套有效的高山夏秋黄瓜栽培技术,以便在同类地区推广应用。     

柑桔越冬后叶片的光合动态

试验用便携式 CO_2红外分析仪对杭州地区温州蜜柑越冬后叶片光合动态进行了测定.结果表明越冬后叶片的净光合速率总趋势是上升,即有越冬后叶片的光合恢复现象.净光合以秋叶最大,夏叶次之,春叶最低.净光合和气温的变化动态有着明显的关系.在越冬后叶片的光合恢复过程中,叶片叶绿素含量提高,表观量子产量上升,而羧化效率几乎不变,表明越冬后叶片光合潜力的恢复主要取决于叶片对光能利用的改善.