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621.
Ambekar E. Eknath Hans B. Bentsen Morten Rye Nguyen Hong Nguyen Bjarne Gjerde 《Aquaculture (Amsterdam, Netherlands)》2007,273(1):1-14
The present study is based on data recorded from fish of the third generation of the GIFT project (Genetic Improvement of Farmed Tilapias). The objective of the study was to compose a synthetic base population of Nile tilapia for further selective breeding, and to estimate phenotypic and genetic parameters in that population. The base population was formed by choosing parent stock among the best performing animals within the best strain combinations resulting from a full diallel cross design (8 × 8) involving four African wild strains and four Asian farmed strains (64 strain combination cells altogether). The grandparent ancestors of the base population were mainly representing the three strains originating from Egypt, Kenya and Thailand (20.2, 27.3 and 19.6%, respectively). The proportion of ancestors from the Senegal strain was medium (12.2%) whereas the proportion of ancestors from Ghana, Israel, Singapore and Taiwan strains was low (3 to 8%) and mainly due to a restriction that all parent strains should be represented in the synthetic base. Statistical analyses using a mixed animal model were carried out on records from 13,570 individually tagged and surviving progeny of 50 sires and 123 dams. The growth performance of the fish was tested in seven diverse environments. The test environments covered a wide range of tilapia farming systems, namely, earthen ponds fertilized with inorganic fertilizer and organic manure or on-farm agricultural residues, cage culture, and test stations located in different agro-climatic regions. The model fitted included the fixed effect of test environment and sex and the linear and quadratic covariate of age at stocking. The random terms were the additive genetic effect of individual fish and the additional effects common to full-sib groups. The estimates of heritability for body weight at harvest in different test environments ranged from moderate to high (0.12 to 0.56) and the estimate across test environments was 0.15. The variances due to other effects common to full-sib families were significant, accounting for 8 to 16% of total variation in body weight. The genetic correlations among body weights recorded in similar environments were high (mostly > 0.80, i.e. among the earthen ponds in experimental locations). By contrast, the genetic correlations between harvest weight in earthen pond and in cage environments were more variable (0.36-0.82). Overall, the results suggested that the genotype by environment interactions were of limited importance, at least for the target pond farming systems in the Philippines. It was concluded that as the additive genetic variation in the base population was large, the harvest weight of the GIFT fish could be effectively improved by selective breeding, and that there was no immediate need to develop separate strains for different environments. 相似文献
622.
Quoc Thinh Nguyen Caroline Douny Minh Phu Tran Francois Brose Phuong Thanh Nguyen Do Thi Thanh Huong Patrick Kestemont Marie‐Louise Scippo 《Aquaculture Research》2019,50(1):247-255
To develop an easy and reliable method for detecting pesticides and their residues in the Mekong Delta, a GC‐MS analytical method was developed and validated according to European guidelines (SANTE/11945/2015) for the determination of residues of three pesticides (quinalphos, trifluralin and dichlorvos) in water. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.002 and 0.007 μg/L, respectively, for quinalphos and trifluralin, and 0.016 and 0.053 μg/L, respectively, for dichlorvos and quinalphos. The repeatability, the within‐laboratory reproducibility as well as the trueness met the European criteria. The recovery rate ranged between 72% (for dichlorvos and quinalphos) and 82% (for trifluralin). The developed method was then applied for the analysis of 33 water samples, collected in April 2013, at the beginning of the rainy season in the Mekong Delta in Vietnam. Thirteen samples were from rice field, 10 were collected from cat fish ponds and from red tilapia cages. Results showed that only 9% of total water samples analysed contained residues of pesticides, but only in water from rice fish systems. From the 13 samples taken in these systems, quinalphos was detected in three samples. The other two pesticides were not detected. A comparison between analytical results obtained from GC‐MS and an alternative method, that is GC‐ECD indicated that GC‐ECD is less sensitive than GC‐MS, with LOQ ranging from 0.37 to 1.18 (depending on the pesticide). However, for samples with concentrations above these LOQ, no significant difference was observed between the results obtained from the two analytical methodologies. 相似文献
623.
Alexandra I Santos Nguyen Hong Nguyen Raul W Ponzoni Hoong Yip Yee Azhar Hamzah Ricardo P Ribeiro 《Aquaculture Research》2014,45(2):353-361
The strain by nutrition interaction in body weight and survival rate was examined by testing three genetic groups (Selection and Control lines of the GIFT strain, and Red tilapia) at two levels of protein in the diet (28% and 34%). The GIFT strain of Nile tilapia (Oreochromis niloticus) has been selected for high‐breeding values for body weight, whereas the Control was contemporaneously maintained and selected for breeding values of body weight close to the population mean. The Red tilapia (Oreochromis spp) was unselected at the time of the experiment. There were a total of 6000 fish at stocking in the study (2000 fish per genetic group). During the grow‐out period of 147 days, within each genetic group, the fish were randomly assigned to either a 28% or a 34% protein diet. Survival rate during grow‐out averaged 72%. A total of 4335 fish were harvested, with individual body measurements and survival recorded. The effect of genetic group accounted for the largest proportion of variation in body weight and survival, followed by sex and protein level. Across the two dietary protein levels, the GIFT strain had the highest growth. The difference in body weight between the Control and Red tilapia was not statistically significant (P > 0.05). Responses in growth to dietary protein levels also differed between genetic groups. No differences (P > 0.05) in body weight were found in the GIFT selection and Control fish fed 28% or 34% protein diets. However, body weight of Red tilapia was greater in the high‐ than in the low‐protein diet. In contrast to body weight, survival rate from stocking to harvest was affected by protein level. The high‐protein diet significantly improved survival rate, averaging 24% across the three strains. The overall results indicate significant effects of genetic group and dietary protein level on both body weight and survival rate; however, the interaction between strain and dietary protein levels was small and possibly unimportant for these traits. It is concluded that the 28% protein diet used in the selection programme for the GIFT strain results in the selection of genotypes that can perform well under commercial feeds. 相似文献
624.
Nguyen Phuc Thuong Edina Prondvai Barbara De Kegel Tania De Wolf Paul Eckhard Witten Dominique Adriaens 《Journal of fish diseases》2019,42(9):1259-1270
In this study, we describe an abnormal ectopically mineralized structure (EMS) that was found inside the skull of a juvenile Sparus aurata that also showed a bilateral opercular deformation. The overall phenotype and tissue composition were studied using micro‐CT scanning and histological analyses. The ectopic structure occupies a large volume of the brain cavity, partially extruding into the gill cavity. It shows a dense mineralization and an extracellular matrix‐rich phenotype, with variation in both the morphology and size of the cell lacunae, combined with an irregular fibre organization inside the matrix. This study is the first to report such an EMS in a juvenile teleost fish, where the tissue does not resemble any other connective tissue type described in bony fish so far. The tissue phenotype seems to rule out that the EMS corresponds to a tumorous cartilage. Yet, it is rather reminiscent of a highly mineralized structure found in cartilaginous fish, where it is suggested to be associated with damage repair. 相似文献
625.
Cuong Dinh Nguyen Shao-Hui Zheng Sachiyo Sanada-Morimura Masaya Matsumura Hideshi Yasui Daisuke Fujita 《Breeding Science》2021,71(5):497
Rice (Oryza sativa L.) yield is severely reduced by the brown planthopper (BPH), Nilaparvata lugens Stål, in Asian countries. Increasing resistance in rice against BPH can mitigate yield loss. Previous reports indicated the presence of three BPH resistance genes, BPH2, BPH17-ptb, and BPH32, in durable resistant indica rice cultivar ‘PTB33’. However, several important questions remain unclear; the genetic locations of BPH resistance genes on rice chromosomes and how these genes confer resistance, especially with relationship to three major categories of resistance mechanisms; antibiosis, antixenosis or tolerance. In this study, locations of BPH2, BPH17-ptb, and BPH32 were delimited using chromosome segment substitution lines derived from crosses between ‘Taichung 65’ and near-isogenic lines for BPH2 (BPH2-NIL), BPH17-ptb (BPH17-ptb-NIL), and BPH32 (BPH32-NIL). BPH2 was delimited as approximately 247.5 kbp between RM28449 and ID-161-2 on chromosome 12. BPH17-ptb and BPH32 were located between RM1305 and RM6156 on chromosome 4 and RM508 and RM19341 on chromosome 6, respectively. The antibiosis, antixenosis, and tolerance were estimated by several tests using BPH2-NIL, BPH17-ptb-NIL, and BPH32-NIL. BPH2 and BPH17-ptb showed resistance to antibiosis and antixenosis, while BPH17-ptb and BPH32 showed tolerance. These results contribute to the development of durable BPH resistance lines using three resistance genes from ‘PTB33’. 相似文献
626.
Khiem Nguyen Minh Takahashi Yuki Oanh Dang Thi Hoang Hai Tran Ngoc Yasuma Hiroki Kimura Nobuo 《Fisheries Science》2020,86(4):673-683
Fisheries Science - Predicting the outbreak of disease is essential when managing shrimp farms. Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus is a serious... 相似文献
627.
628.
Nguyen Thanh Vu Nguyen Van Sang Trinh Quoc Trong Nguyen Huynh Duy Nguyen Thi Dang Nguyen Hong Nguyen 《Journal of fish diseases》2019,42(10):1409-1417
Bacillary necrosis of Pangasius (BNP), caused by Edwardsiella ictaluri, is one of the most devastating diseases in striped catfish farming. To date, quantitative genetic inheritance of BNP resistance is not known in striped catfish Pangasianodon hypophthalmus. The main aim of this study was to estimate genetic parameters for BNP resistance in a breeding population of striped catfish undergoing four generations of selection for high growth. Specifically, the study examined whether BNP resistance is heritable to enable family selection and whether genetic improvement for enhanced BNP resistance may have detrimental effects on growth and survival rate. To test these hypotheses, 720 full‐ and half‐sib families were challenged with E. ictaluri pathogen using injection and cohabitation methods over four years, from 2010 to 2012 and 2015. In total, the data included 398,234 animals in the pedigree, from which 18,849 animals had disease challenge test records and 39,103 siblings had growth performance. Both univariate and bivariate sire–dam linear and threshold mixed models were used to estimate (co)variance components for BNP resistance, survivals and growth traits. The estimates of heritability for the BNP resistance recorded as death or survival were low regardless of models used (0.10–0.16), whereas survival time (days post‐challenge test) showed moderate heritability (0.35). The survival rate during hapa rearing had medium heritability (0.33–0.52). The genetic correlations of BNP resistance with body weight and survival were all positive (0.03–0.53), suggesting that selection of increased BNP resistance may have positive impacts on growth and survival traits, and these traits could be easily improved simultaneously in the selective breeding programme for striped catfish. 相似文献
629.
Kim T. Tran‐Ngoc Son T. Huynh Joo Sendo Thinh H. Nguyen Arjen J. Roem Johan A. J. Verreth Johan W. Schrama 《Aquaculture Nutrition》2019,25(1):134-144
The impact of two dietary organic acids (OAs) on nutrient digestibility and intestinal morphology was determined in Nile tilapia under conditions of dissolved oxygen in the water: normoxia and hypoxia. Four diets designated as control (0 g/kg organic acid salt), KDF (2 g/kg potassium diformate), CAB (2 g/kg calcium butyrate) and their combination (4 g/kg of a mixture of KDF and CAB, ration 1:1) were formulated with 520 g/kg of soybean meal in order to produce soybean meal enteritis‐like symptoms. The four diets were tested first under normoxic conditions (6 mg/L) for a period of 5 weeks, followed by a test period under hypoxic conditions (3 mg/L). The results showed that OAs were unable to significantly improve growth and nutrient digestibility under normoxic conditions but under hypoxic conditions, there was a significant enhancement of the growth and nutrient digestibility. Fish fed OA‐supplemented diets showed improvements in the intestinal morphology under the normoxic conditions, and these effects were more pronounced under the hypoxic conditions. Experimental findings suggest that OAs can improve the nutrient digestibility and intestinal morphology under hypoxic conditions. A synergistic effect by the combination of formic and butyric acid on growth, digestibility and intestinal morphology was not found. 相似文献
630.
Thang Nguyen Dinh Tu Le Dinh Le Na Nguyen Thi Trang Ngo Thi Nghia Phan Tuan 《International Aquatic Research》2019,11(3):303-310
International Aquatic Research - Viral diseases are a serious issue for the shrimp aquaculture industry. White spot syndrome virus (WSSV) has been considered one of the most dangerous pathogens... 相似文献