全文获取类型
收费全文 | 235篇 |
免费 | 6篇 |
专业分类
林业 | 32篇 |
农学 | 7篇 |
56篇 | |
综合类 | 7篇 |
农作物 | 13篇 |
水产渔业 | 19篇 |
畜牧兽医 | 88篇 |
园艺 | 4篇 |
植物保护 | 15篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 7篇 |
2014年 | 8篇 |
2013年 | 19篇 |
2012年 | 15篇 |
2011年 | 21篇 |
2010年 | 9篇 |
2009年 | 11篇 |
2008年 | 20篇 |
2007年 | 20篇 |
2006年 | 17篇 |
2005年 | 12篇 |
2004年 | 13篇 |
2003年 | 13篇 |
2002年 | 12篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有241条查询结果,搜索用时 31 毫秒
41.
Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil 总被引:2,自引:0,他引:2
42.
43.
Matsuda F Inoue N Goto Y Maeda A Cheng Y Sakamaki K Manabe N 《The Journal of reproduction and development》2008,54(5):314-320
More than 99% of follicles in mammalian ovaries undergo atresia, but the mechanisms regulating the strict selection process are still unclear. Granulosa cell apoptosis is considered the trigger of follicular atresia, which occurs in advance of the death of an oocyte. Cellular FLICE-like inhibitory protein (cFLIP), a homologue of procaspase-8 (also called FLICE), is an intracellular anti-apoptotic protein. It is expressed in granulosa cells of porcine ovaries, where its levels decreases during follicular atresia. We hypothesized that cFLIP regulates granulosa cell apoptosis by acting as a pro-survival factor. In the present study, to further reveal the function of cFLIP in granulosa cells, we examined the anti-apoptotic mechanism of cFLIP using KGN, a human granulosa tumor cell line. Fas-mediated apoptosis was induced by co-treatment with anti-Fas antibody (CH-11), which acts as an agonist of Fas-ligand, and cycloheximide (CHX). When cFLIP was stably expressed in KGN cells following transfection of an expression vector, the Fas-mediated apoptosis was inhibited. Suppression of cFLIP by small interfering RNA (siRNA) spontaneously induced cell death. Silencing of cFLIP promoted cleavage of procaspase-8, and the cell death caused by cFLIP siRNA was completely blocked by a caspase-8 inhibitor (Z-IETD-FMK), indicating that cFLIP regulates apoptosis in KGN cells by inhibiting cleavage of procaspase-8. In conclusion, cFLIP is an essential pro-survival factor for granulosa cells, and it prevents granulosa cell apoptosis by inhibiting procaspase-8 activation. 相似文献
44.
45.
It is known that soil property varies along the slope. It suggests that soil solution chemistry also differs topographically.
To determine the variation in soil solution chemistry within one watershed, soil solution chemistry at the different positions
of the slope was investigated. Soil N transformation changed along the slope. NH4
+ ratio to inorganic N (NH4
+ + NO3
−) increased upslope. The tendency was verified by laboratory incubation. After incubation most of the mineralized N was nitrified
at the lower part of the slope, while little nitrification occurred at the upper part of the slope. At the ridge and the backslope
inorganic N form in soil solution was concomitant with inorganic N form by incubation. At the ridge NH4
+ was predominant form in soil solution, at that time major anion was sea salt originated Cl−. From this, soil solution chemistry seems to be regulated by the external nutrient source at the ridge. In the second year
of lysimeter installation NO3
− concentration increased in both sites and the ratio of NH4
+ to inorganic N decreased. It was considered due to the effect of lysimeter installation. The lag time and the magnitude of
NO3
− increase were different between the ridge and the backslope. It would be related with soil N transformation in pre-disturbance.
The influence of disturbance were shown in other solute concentrations of soil solution. 相似文献
46.
Masahiro Natsume Asaho Nagagata Marja Aittamaa Naoko Okaniwa Panu Somervuo Hans-Peter Fiedler Jan F. Kreuze Veli-Matti Rokka Hans Bång Hiroshi Kawaide Jari P. T. Valkonen 《Journal of General Plant Pathology》2018,84(2):108-117
Streptomyces spp. are a highly diverse group of bacteria most of which are soil-inhabiting saprophytes. A few are plant pathogens that produce a family of phytotoxins called thaxtomins and cause significant economic losses, e.g., by reducing the marketability of potato tubers (Solanum tuberosum). In northern Europe, S. scabies, S. turgidiscabies and S. europaeiscabiei are the most common plant pathogenic species. In this study, a Streptomyces strain isolated from a netted scab lesion on a tuber of potato cv. Bintje in northern Sweden was identified as S. turgidiscabies but was found to differ in the genomic region carrying genes required for thaxtomin biosynthesis. Our results showed that the strain did not produce thaxtomin but rather phytotoxin fridamycin E, which is an anthraquinone novel to plant pathogenic Streptomyces spp. Fridamycin E was shown to reduce or inhibit sprouting of potato microtubers in vitro. While fridamycin E is known to have antibiotic activity against Gram-positive bacteria, the inhibitory activity of fridamycin E on plant growth is a novel finding. 相似文献
47.
Kazuya Iizuka Narumi Toya Jyunichi Ohshima Futoshi Ishiguri Naoko Miyamoto Mineaki Aizawa Tatsuhiro Ohkubo Chisato Takenaka Shinso Yokota 《Journal of Wood Science》2018,64(1):59-64
To utilize forest resources in areas affected by fallout from the Fukushima Daiichi Nuclear Power Plant accident, it is important to understand the mechanisms of 137Cs movement through the stem wood of contaminated trees. Understanding the mechanism of absorption and migration of 137Cs to stem wood is necessary for clues to the future prediction of the transition of 137Cs to xylem. In the present study, radial variations in 137Cs concentration were investigated in Japanese cedar (Cryptomeria japonica D. Don) trees collected 1 year and 10 months after the accident. Additionally, the relationship between 137Cs concentration and potassium (K) content was established. Trees with a higher moisture content and lower lightness value in heartwood tended to have a higher 137Cs concentration in the heartwood. In these trees, 137Cs concentration peaked at the heartwood–sapwood boundary and gradually decreased toward the pith. By contrast, K content within the heartwood remained nearly constant along the radial direction. The heartwood-to-sapwood ratio of 137Cs concentration was significantly positively correlated with that of K content. Based on these results, we suggest that 137Cs movement from sapwood to heartwood might be related to the K content ratio of heartwood and sapwood. 相似文献
48.
Winter nitrogen use in deciduous species is largely uncharacterized. We investigated nitrate uptake in the fine roots of a
deciduous oak (Quercus serrata Thunb. ex. Murray). We conducted a 15N-labeling experiment using saplings of Q. serrata in the winter. During three weeks of labeled nitrate application, the concentration of 15N in the fine roots increased significantly. The amount of nitrogen absorbed, as nitrate, was 1.16 ± 1.02 mg N g DW−1, equivalent to 7.6 ± 5.8% of the total nitrogen content. Our results indicate that Q. serrata saplings have significant potential for nitrate uptake in the fine roots in midwinter (i.e., in the absence of leaves). Although
a significant amount of nitrogen applied as nitrate was accumulated, nitrate concentration in the fine roots remained low
during the labeling period. Furthermore, significant nitrate reductase activity was detected. These data suggest that Q. serrata saplings can assimilate nitrate in the fine roots in midwinter. 相似文献
49.
Kenji Tsuruta Tomonori Kume Hikaru Komatsu Naoko Higashi Toshihiro Umebayashi Tomo’omi Kumagai Kyoichi Otsuki 《Journal of Forest Research》2010,15(6):398-403
Sap flow techniques are practical tools for estimating tree transpiration. Though many previous studies using sap flow techniques
did not consider azimuthal variations of sap flux density (F
d) on xylem trunk to estimate tree transpiration, a few studies reported that ignoring the azimuthal variations in F
d could cause large errors in tree transpiration estimates for some tree species. Therefore, examining azimuthal variations
in F
d for major plantation tree species is critical for estimating tree transpiration. Using the thermal dissipation method, we
examined azimuthal variations in F
d in six trees of Japanese cypress Chamaecyparis obtusa (Sieb. et Zucc.) Endl., which is one of the most common plantation tree species in Japan. We recorded considerable variations
among F
d at four different azimuthal directions. The F
d value for one aspect was more than 100% larger than those for the other aspects. We calculated differences between tree transpiration
estimates based on F
d for one to three azimuthal directions and those based on F
d for four aspects. The differences relative to tree transpiration estimates based on F
d for four aspects were typically 30, 20, and 10% in accordance with the F
d for one, two, and three measurement aspects, respectively. This finding indicates that ignoring azimuthal variations could
cause large errors in tree transpiration estimates for Japanese cypress. 相似文献
50.
Sawa S Cherrier M Lochner M Satoh-Takayama N Fehling HJ Langa F Di Santo JP Eberl G 《Science (New York, N.Y.)》2010,330(6004):665-669
Lymphoid tissue-inducer (LTi) cells initiate the development of lymphoid tissues through the activation of local stromal cells in a process similar to inflammation. LTi cells express the nuclear hormone receptor RORγt, which also directs the expression of the proinflammatory cytokine interleukin-17 in T cells. We show here that LTi cells are part of a larger family of proinflammatory RORγt(+) innate lymphoid cells (ILCs) that differentiate from distinct fetal liver RORγt(+) precursors. The fate of RORγt(+) ILCs is determined by mouse age, and after birth, favors the generation of cells involved in intestinal homeostasis and defense. Contrary to RORγt(+) T cells, however, RORγt(+) ILCs develop in the absence of microbiota. Our study indicates that RORγt(+) ILCs evolve to preempt intestinal colonization by microbial symbionts. 相似文献