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41.
微流控芯片是一项以芯片为基础,结合化学、物理学、生物学等多学科形成的技术平台,能够在一个芯片上实现样品制备、反应、分离、检测等流程,具有高通量、高效率和易操作的特点。随着材料科学和纳米技术的发展,该项技术得到迅速发展并应用于化学分析、微生物检测等,尤其是在微生物检测和分析方面发挥着重要作用,并逐步产生了商品化的微流控芯片。微流控芯片因具有小型化、节约试剂、速度快、集成化程度高等优点,适用于处理突发公共卫生事件,但目前在动物疫病检测中的应用还很少见。本文对微流控芯片的技术原理、结构及其在微生物检测中的应用进行综述,以期为动物疫病的高通量检测提供借鉴。 相似文献
42.
采用人工注射催产药物获取受精卵、人工孵化等技术原理,在不同的盐度中进行亲鱼培育、人工催产、人工孵化和苗种培育对比试验研究,重点解决了催产时间、催产药物的选择等技术问题,同时研究出各个时期所需要的最佳盐度。 相似文献
43.
Hiromitsu Furuya Tsutomu Matsumoto Shin-ichi Fuji Hideki Naito 《Journal of General Plant Pathology》2003,69(2):115-119
Rice seedling growth, estimated by plant height and root development and discoloration, was better in pasteurized soil than
in unpasteurized soil obtained from a flooded rice field. Rice seedlings also grew better in sterilized soil modified by adding
roots harvested from the pasteurized soil than in soil modified by adding roots harvested from the unpasteurized soil. The
results demonstrate that seedling growth in the rice field soil was inhibited by soil microorganisms, even though no typical
symptoms such as seedling blight or damping-off appeared. Pythium aristosporum is suggested to be involved in the inhibition. Thus, it appears that inconspicuous restraint of rice seedling growth could
occur in soils of rice paddy fields.
Received: May 20, 2002 / Accepted: October 16, 2002
Acknowledgments The authors thank Dr. T. Ichitani, former professor at Osaka Prefectural University, for providing an isolate of Pythium aristosporum for comparison, and Mr. Mitsuaki Sato of Akita Prefectural College of Agriculture for technical assistance. 相似文献
44.
锦屏县毛竹现状分析与产业发展对策 总被引:1,自引:0,他引:1
毛竹产业的发展必须以市场为导向,以增加农民收入和促进山区农村经济发展为目标,创新发展方式,加大扶持力度,按照规模上求大、品种上求新、管理上求精的要求,坚持扩大面积与提高种植质量并重,强化科技支持与产业经营体系建设,把毛竹子产业培育成山区经济发展的重要支柱产业。 相似文献
45.
Sun Lifei Sang Changpeng Wang Chao Fan Zhenzhen Peng Bo Jiang Ping Xia Zongwei 《Journal of Soils and Sediments》2019,19(10):3499-3511
Journal of Soils and Sediments - The responses of N2O emission to increasing temperature in different soil horizons are not clearly understood yet. Here, we investigated the effects of increasing... 相似文献
46.
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48.
Qing-Qin Cao Yi-Chen Jiang Xing-Liang Li Yuan-Yue Shen Yu Xing Ke-Feng Fang 《The Journal of Horticultural Science and Biotechnology》2013,88(6):726-732
SummaryRetrotransposons are major components of the genomes of most eukaryotic organisms and have resulted in the introduction of desirable traits in many crops, including fruit trees. Here, we describe a Ty3-gypsy-like retrotransposon associated with a short-catkin mutant in Chinese chestnut (Castanea mollissima), resulting in catkins that are < 20% the length of normal staminate catkins. A partial sequence of the retrotransposon, named CmRT1, detected by amplified fragment length polymorphism (AFLP) analysis, and its complete sequence were determined from the genome of Chinese chestnut (Castanea mollissima) using improved Tail-PCR. CmRT1 was 10,067 bp in length and shared high homology in its predicted amino acid sequence and motifs with other Ty3/gypsy-like retrotransposons. The 5’ long terminal repeat (LTR) of CmRT1 contained a TATA box and several cis-elements that were predicted to be important for processes involving abscisic acid, gibberellic acid, and auxins and in stress-mediated responses. Further characterisation of the transposition event that led to the short-catkin phenotype was performed using two pairs of primers that aligned with the flanking region of the LTRs. The expected PCR bands were observed only in genomic DNA from plants that showed the mutation. Finally, cloning and real-time qPCR analysis of an NADP-dependent alkenal double-bond reductase (CmADBR) target gene that was adjacent to CmRT1, revealed that CmADBR expression was significantly down-regulated in the short-catkin mutant. Taken together, these results suggest that the CmRT1 retrotransposon is responsible for the short-catkin phenotype. 相似文献
49.
50.
Manjeet B. L. Pander R. Sharma S. S. Dhaka Ankit Magotra Kapil Dev 《Tropical animal health and production》2017,49(8):1689-1695
Foot and mouth disease (FMD) is the most contagious disease of mammals and a major threat to animal husbandry sector. In India, vaccination with the inactivated trivalent (O, A and Asia1) vaccine is one proven way for protecting the livestock from FMD. However, many outbreaks have been reported in different parts of the country. Therefore, present study was aimed at elucidating the effects of genetic and non-genetic factors on FMD viral vaccine-elicited immune response in Hardhenu cattle. The effect of season of vaccination was not consistent. The effect of status of animal was significant for all the pre and post AB titres except for pre AB titre of serotype O and post AB titre of Asia1.The estimates of heritability for response to vaccination were low to high ranging from 0.11 to 0.45. The highest heritability estimate was obtained for serotype O and the lowest for Asia1. The heritability estimates for pre and post AB titres ranged from 0.15 to 0.33. All the pre and post AB titres and responses to vaccination had genetic correlations ranged from high negative to high positive among them. Results of this study highlight the variation in vaccine response which needs to be further exploited on large-scale animal data for better immunization and protection against highly contagious viral vesicular disease of cloven-hoofed animals. 相似文献