诱导Paracin1.7生成的刺激因子的分离与纯化

旨在分离纯化枯草芽孢杆菌(Bacillus subtilis)中能够诱导细菌素Paracin1.7分泌的刺激因子,并探明其对副干酪乳杆菌(Lactobacillus paracasei) HD1.7细菌素生成量及群体感应相关基因luxS表达的影响。本研究通过60%硫酸铵沉淀、CM Sepharose Fast Flow弱阳离子交换柱层析中度纯化及Superdex 75凝胶层析精度纯化,将获得的层析分离物与L. paracasei HD1.7共培养,检测共培养发酵液的抑菌活性及luxS基因的转录水平,并用SDS-PAGE与Native-PAGE检测分离物质。结果表明L. paracasei HD1.7抑菌活性为126.68%;luxS基因上调表达,为对照菌株的2.43倍;刺激因子的表观分子量约为30 kDa。本研究利用三步法初步分离纯化出诱导Paracin1.7生成的刺激因子,明确该刺激因子可以启动种间群体感应相关基因luxS,为L. paracasei HD1.7的群体感应研究奠定了理论和技术基础。     

Functional porous carbons from waste cotton fabrics for dyeing wastewater purification

A new functional porous carbons (PC-WF) is prepared by activation-pyrolysis method use waste cotton fabrics (WF) as an abundant, cheap and available precursor for removal of Brilliant Crocein (BC-GR) and Cationic Red 2GL (CR-GL) from aqueous phase. The PC-WF was characterized by BET, FTIR, SEM, and XRD techniques, the surface area, total pore volume, average pore diameter was found as 1463.5 m² g^-1, 0.783 cm³ g^-1 and 2.14 nm, respectively. The influences on BC-GR and CR-GL adsorption of various experimental factors such as initial concentration and temperature were investigated. Adsorption kinetics was found to be best represented by the pseudo-second order model. The adsorption capacity was 319.8 mg g^-1 for BC-GR and 842.5 mg g^-1 for CR-GL at 30 °C, respectively. The results indicate that for waste cotton fabrics in particular, the practical application of this process to the production of porous carbon would be possible.     

Mapping QTL of Fiber Yield and Quality Traits in F2 Populations of Chromosome Segment Substitution Lines from Gossypium hirsutum × Gossypium barbadense

In this study, F₂ populations from two chromosome segment substitution lines MBI7455 and MBI7358, were quantified using SSR to evaluate the parents' genotype and detect QTL related to fiber quality plus yield traits of cotton. Results show that the recurrent parent (CCRI45) hosted 96.70% and 95.60% of chromosome segment substitution in MBI7455 with 12 chromosome segments and in MBI7358 with 16 chromosome substitution segments of Gossypium barbadense, respectively. In the F₂ population, the average rate of chromosome substitution of the recurrent parent (CCRI45) was 96.44%, and the average segments of chromosome substitution of Gossypium barbadense was 13.42, with an average segments of homozygous donor chromosome value of 3.90. Analysis showed 19 fiber quality-related QTL with a phenotypic variance of between 2.52%-13.11% and seven yield traits-related QTL with a range of 2.93%-11.40% phenotypic variance, resulting in a total of 26 QTL. The CSSLs could be used to detect QTL for fiber yield and quality traits, which offer an important foundation for the cotton molecular-assisted breeding.