黄波罗小蘖碱含量的种源试验

黄波罗树皮即中药黄柏,具有抑菌、抗病毒、降压、镇咳和祛痰等功效,其主要成分为小蘖碱.本文选取带岭、帽儿山、苇河三个种源的黄波罗各6株,利用高效液相色谱测定小蘖碱含量,调查小檗碱含量种源变异情况及其在黄波罗植株内的分布情况,结果表明,每个种源各部位小檗碱平均含量差异不明显;而不同部位小檗碱含量有明显差异,各个部位含量大小为根部〉树皮〉枝皮〉叶部.     

镉胁迫对矮生四季豆种子萌发和幼苗生长发育的影响

以水培法研究了不同浓度的Cd2＋溶液对四季豆种子萌发和幼苗生长发育的影响。结果表明：当Cd2＋浓度较低时,Cd2＋对种子萌发及幼苗生长发育毒害作用较小,一定浓度范围内（0.05～0.5mg/L）还有促生作用,高浓度Cd2＋(5～100mg/L)能严重抑制四季豆幼苗根及下胚轴的生长;Cd2＋胁迫对四季豆幼苗体内过氧化物酶 (POD),超氧化物歧化酶（SOD）,过氧化氢酶（CAT）活性存在低浓度激活和高浓度抑制的效应,且同一浓度的Cd2＋对四季豆幼苗POD、CAT活性的抑制作用表现为根系大于下胚轴,Cd2＋浓度＞0.5mg/L时,根系SOD活性大于下胚轴中的活性;Cd2＋胁迫下,四季豆下胚轴和根的丙二醛（MDA）含量增加,且根部含量大于下胚轴的量。     

拟青山海关杨高效遗传转化系统的建立

本实验以叶片为外植体,建立了拟青山海关杨的高频再生系统;在此基础上,利用GUS瞬时表达法研究了菌种、预培养时间、侵染菌液浓度、侵染时间和乙酰丁香酮（AS）浓度5个因素对拟青山海关杨叶片遗传转化的影响。结果表明,拟青山海关杨叶片最适不定芽诱导培养基为MS添加0.5mg/L的6-BA 和0.1mg/L的NAA,叶片再生频率为95%,平均不定芽数为10.07个;该杨树的高效遗传转化体系为：叶片省去预培养,菌种使用GV3101,侵染液OD600为 0.4～0.6,侵染10min,共培养培养基中添加乙酰丁香酮 200μmol/L,在此条件下,GUS荧光定量分析结果显示叶片的GUS活性达到最大值。     

变叶海棠的研究进展与应用前景

变叶海棠为我国西部高海拔地区植被恢复优良乡土树种,属蔷薇科苹果属植物,其果实具有很高的营养价值,叶片可制茶,是一种天然的绿色保健品。本文综述了变叶海棠生物学特性、植物区系、居群特征、起源、遗传多样性、形态学、抗逆性及栽培利用方面研究现状。提出野生变叶海棠资源现状调查、种质保存、人工扩繁技术、抗旱机理以及市场开发利用等研究亟待加强,同时展望了变叶海棠的市场开发前景。     

抛栽密度与促芽肥用量和化学促芽剂对中稻—再生稻的影响研究

此研究是针对再生稻生产中存在发苗不足、成穗率低、肥料利用率不高等因素而设计。试验结果表明,抛栽密度在18～31.5万穴/hm^2的范围内,再生稻粒重变化不大,实际产量基本一致,但再生稻发苗数和有效穗以18～22.5万穴/hm^2最高,分别达20.3～24.3苗/穴和18.9～20.1穗/穴;促芽肥施用过多,会影响头季中稻的产量,随促芽肥的增加,再生芽长也增长,但发苗数、有效穗数、结实率、单穴穗重则有下降趋势。同时,化学促芽剂4PU-30、高产精、腐殖酸、IAA能够增加头季中稻单穗重和促进再生芽的萌发生长,提高穗数和产量。     

Routing protocol of the wireless sensor networks for bridge health monitoring

By analyzing the reason that why the existing routing protocols cannot be applied to the wireless sensor networks for bridge health monitoring, a new routing protocol is proposed. Since the locations of the collecting modules are fixed, the proposed protocol exchanges the routing information between the neighbor nodes by adjusting the exchange cycle according to reliability of the modules. For the low routing security requirement, while to increase the routing efficient, the middle nodes replying the routing requirements with variable thresholds is introduced. In order to make full use of information in route discovering procedure, the backup routes mechanism is adopted. The proposed protocol can efficiently forward data and is suitable for the bridge health monitoring.     

低甲氧基果胶制备草莓低糖果酱的研究

采用单因素和正交试验设计,以草莓低糖果酱的感官评分为评价指标,并结合质构仪进行凝胶强度的辅助评价,研究低甲氧基果胶制备草莓低糖果酱的最佳工艺条件。结果表明,草莓低糖果酱制备最佳工艺条件为:以草莓原料为基准,低甲氧基果胶用量为0.3%,CaCl2用量为1.6mg/g,加糖量为20%。     

Methyl jasmonate alleviated salinity stress in soybean

We studied the role of methyl jasmonate (MeJA) in alleviating NaCl-induced salt stress on soybean growth and development in hydroponics medium. Soybean seedlings were exposed to 60 mM NaCl stress for 2 weeks, 24 h after the application of 20 and 30 μM MeJA. NaCl stress induced a significant reduction in plant growth, endogenous bioactive gibberellin (GA4), photosynthesis and transpiration rate, while a marked increase in the endogenous abscisic acid (ABA) and proline contents were recorded. MeJA application greatly mitigated the adverse effects of NaCl on soybean growth and endogenous hormones. MeJA significantly increased ABA levels, while the endogenous amount of GA4 was reduced by the application of NaCl. Our study revealed that MeJA counteracted the negative effects of NaCl stress on plant growth, chlorophyll content, leaf photosynthetic rate, leaf transpiration rate, and proline content.     

Density dependence on tree survival in an old-growth temperate forest in northeastern China

? Density dependence is a major mechanism for shaping plant communities. However, its role in regulating diverse, mixed natural tree communities is less certain.

? In this study we investigated density-dependent effects in a large-scale (25 ha) old-growth temperate forest in northeastern China. Spatial patterns of neighborhood distribution in the plot were analyzed using various methods for inferring competition, including (1) pair correlation function to determine spatial patterns of pre-mortality and post-mortality and (2) neighborhood analysis of individuals to examine the extent to which tree survival is correlated with other covariates.

? Results showed that, for common species, 3 of 5 canopy species and 3 of 8 midstory and understory species were random in mortality. Negative density-dependent mortality was not found when trees reach 1 cm in DBH. There was no significant correlation for canopy species between tree survival and conspecific abundance, but largely positive correlations for midstory and understory species. In contrast, tree survival was found to negatively correlate with conspecific basal area for most species, indicating strong intraspecific competition. No strong interspecific density dependence was found in the forest.

     

N+离子注入对萌发期小麦中POD和SOD同工酶的影响

采用垂直板聚丙烯酰胺凝胶电泳技术,研究N+离子注入处理后小麦种子豫麦34萌发时期根、盾片、芽、胚乳中POD和SOD同工酶的变化.结果表明:N+离子注入可引起豫麦34的不同器官中POD和SOD同工酶的酶带与酶活性的不同变化,除芽中POD和SOD同工酶活性无明显变化外,其它器官中POD和SOD同工酶均表现为活性增强.4种器官相比,根和胚乳中同工酶变化较大,盾片和芽中同工酶变化不明显.其中,根中同工酶变化更为显著,N+离子束注入处理后根中POD同工酶多了Rf值为0.47的新酶带.