Impacts of conservation buffers and grasslands on total phosphorus loads using hydrological modeling and remote sensing techniques

To better assess the impacts of conservation buffers and grasslands on water quality at large spatial scales, development and integration of novel approaches are crucial to ensure that these land management practices are functioning properly and meeting their original goals. Recent developments in remote sensing technology have greatly enriched the availability of geospatial data that can be used in hydrological modeling to assess the potential hydrological response of conservation practices over larger areas. A methodology was developed using the object-based image analysis approach with Landsat-5 TM imagery of the year 2005 and thematic layers of streams to quantify conservation buffers and grasslands (OBIA-2005). The OBIA-2005 land cover data was used in the Soil and Water Assessment Tool hydrologic model to assess the impacts of vegetative conservation practices on total phosphorus (TP) loads. The model was calibrated and validated for discharge and TP loads in the Cedar Creek Watershed (CCW) in northeast Indiana. In general model efficiency for streamflow values was within acceptable statistical ranges. While calibration of TP loads was satisfactory for the total contributing area of two nested catchments within the upper CCW. Vegetative buffers of 30.5 m and 61 m combined with conservation grasslands generated from the OBIA-2005 resulted in a large reduction of TP loads as compared to no practices. The results also showed that including conservation grassland alone reduced TP loads by less than 2%. However, the combination of these practices with the width of edge-of-field buffer strips module of the SWAT model achieved the largest TP loads reduction. These findings demonstrate that improved representation of vegetative conservation practices in geospatial land cover data sets are more effective in assessing the impacts of conservation buffers and grasslands on water quality through hydrologic modeling.     

Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase

A major cause of aging is thought to result from the cumulative effects of cell loss over time. In yeast, caloric restriction (CR) delays aging by activating the Sir2 deacetylase. Here we show that expression of mammalian Sir2 (SIRT1) is induced in CR rats as well as in human cells that are treated with serum from these animals. Insulin and insulin-like growth factor 1 (IGF-1) attenuated this response. SIRT1 deacetylates the DNA repair factor Ku70, causing it to sequester the proapoptotic factor Bax away from mitochondria, thereby inhibiting stress-induced apoptotic cell death. Thus, CR could extend life-span by inducing SIRT1 expression and promoting the long-term survival of irreplaceable cells.     

Femtotesla magnetic field measurement with magnetoresistive sensors

The measurement of magnetic fields in the femtotesla (fT, 10(-15) tesla) range is important for applications such as magnetometry, quantum computing, solid-state nuclear magnetic resonance, and magnetoencephalography. The only sensors capable of detecting these very small fields have been based on low-temperature superconducting quantum interference devices operating at 4.2 kelvin. We present a magnetic field sensor that combines a superconducting flux-to-field transformer with a low-noise giant magnetoresistive sensor. The sensor can be operated up to 77 kelvin. Our small-size prototype provides the capability of measuring 32 fT.     

Protective socking material for cultivated mussels: a potential non-disruptive deterrent to reduce losses to diving ducks

Predation of cultivated mussels by diving ducks can threaten the viability of mussel farms. Conventional scaring tactics have had limited success at deterring ducks from feeding on cultivated mussels, because of rapid habituation and 24-h feeding cycles of some waterfowl species. We tested a socking material containing a biodegradable protective layer against predation by diving ducks on experimental blue mussel (Mytilus edulis) aquaculture sites in Prince Edward Island, Canada. We also assessed the effect of this new socking material on mussel growth and survival. Results showed that the protective socking has the potential to reduce losses to ducks, but did not perform well for all mussel seed sizes used by the industry. The protective layer also did not biodegrade fast enough, trapping a portion of mussels inside the sock, affecting growth and survival of these mussels. Improvements are needed to make this solution effective for all mussel sizes used by the industry and to make its production more cost effective.     

Effects of seed size and density on growth, tissue-to-shell ratio and survival of cultivated mussels (Mytilus edulis) in Prince Edward Island, Canada

In suspended aquaculture, the size of mussel seed and the density at which these mussels are placed in socks are two important factors affecting mussel production. However, the effect of these two factors has yet to be examined simultaneously. We conducted two large-scale field experiments to assess the effect of seed size and initial density on growth, tissue-to-shell ratio and survival of blue mussels (Mytilus edulis) grown on longlines. Experimental mussel sites were set up during the autumn of two consecutive years in two bays on the north shore of Prince Edward Island, Canada. At each site, socks containing combinations of three seed sizes (small, medium and large) at two initial densities (low and high) were deployed and monitored over time. Small seed grew faster than large seed and often reached commercial size in the same time period. Furthermore, initial density generally did not affect shell growth. During the pre-spawning period following the autumn deployment, seed at low initial density had a higher tissue-to-shell ratio than seed at high initial density, especially for large seed. This effect of initial density disappeared in early summer. Early in the experiments (< about 10 months), mussel survival on socks was affected by seed size only: small seed generally had lower survival rates than larger seed. Late in the experiments (> 10 months), there appeared to be an interaction between seed size and initial density on mussel survival: survival of small seed generally decreased with increasing initial density, while survival of large seed was not affected by initial density. Since large seed tended to be packed at lower initial density than small seed, lack of crowding was likely responsible for the absence of density-dependent loss. Our study thus assessed different seeding strategies, and results were generally consistent across years and sites.     

Estimation of soil classes and their relationship to grapevine vigor in a Bordeaux vineyard: advancing the practical joint use of electromagnetic induction (EMI) and NDVI datasets for precision viticulture

Precision Agriculture - Working within a vineyard in the Pessac Léognan Appellation of Bordeaux, France, this study documents the potential of using simple statistical methods with...     

Genetic diversity among olive varieties of Southern Italy and the traceability of olive oil using SSR markers

Summary

To evaluate germplasm variability and to identify DNA profiles useful for tracing the genetic identity of olive oil, we used six Simple Sequence Repeats (SSRs) in 47 cultivated olive varieties from Central and Southern Italy. A total of 80 polymorphic SSR markers were scored and both the observed heterozygosity and the polymorphic index content were, on average, high. Genetic similarities were investigated by agglomerative hierarchical clustering and principal component analysis. The results implied that most of the olive accessions from the Campania region were genetically different from those of other Italian regions. This finding was further supported by partitioning the genetic variability using analysis of molecular variance. Furthermore, we analysed the DNA isolated from the fruit and mono-varietal oils of three cultivars. Comparative analysis of the SSR profiles revealed that these were conserved in the agro-food chain, although our data also suggested that some issues, such as the sensitivity and performance of the assay in complex mixtures, may pose limitations. Our findings extend current knowledge of Italian olive germplasm and highlight the richness and specificity of the genetic resource of olives in some regions of Southern Italy. They also provide molecular information that can be exploited for the protection of genetic material and mono-varietal oils.     

SITEs revisited: Socioeconomic and demographic contours of small island tourist economies

This study attempts to do three things: (i) provide a review of recent advances in the small island economy literature using the tourism penetration index, (ii) update the impact of tourism across the same global sample of 36 small islands (less than 1 million in population and 5000 km² in area) introduced in APV 47 (1) 2006, and (iii) employing an abbreviated version of the destination life cycle as a development paradigm, construct three different socioeconomic and demographic profiles based on low, intermediate and high tourism impact. Results using cross‐sectional data of 27 indicators reveal three statistically distinct profiles that demonstrate three stages of economic development. Specifically, the findings contrast the low‐income, labour‐exporting, least tourism‐penetrated MIRAB‐type (Migrant/Remittances and Aid/Bureaucracy) emerging islands with their more advanced intermediate impact neighbours. In turn, the latter fall at a significant distance from the most successful s mall i sland t ourist e conomy (SITE) destinations. Thus, this study further refines the characteristics of SITE islands and confirms the viability of tourism in general and the destination life cycle in particular as an effective engine and model, respectively, of island development.     

Effect of Antioxidants During Bovine In Vitro Fertilization Procedures on Spermatozoa and Embryo Development

Increased amounts of reactive oxygen species (ROS) during in vitro fertilization (IVF) may cause cytotoxic damage to gametes, whereas small amounts of ROS favour sperm capacitation. The aim of this study was to investigate the effect of antioxidants [50 μ m β-mercaptoethanol (β-ME) and 50 μ m cysteamine (Cyst)] or a pro-oxidant (5 m m buthionine sulfoximine) on the quality and penetrability of spermatozoa into bovine oocytes and on the subsequent embryo development and quality when added during IVF. Sperm quality, evaluated by the integrity of plasma and acrosomal membranes, and mitochondrial function, was diminished (p < 0.05) after 4-h culture in the presence of antioxidants. Oocyte penetration rates were similar between treatments (p > 0.05), but antioxidants adversely affected the normal pronuclear formation rates (p < 0.05). The incidence of polyspermy was high for β-ME (p < 0.05). No differences were observed in cleavage rates between treatments (p > 0.05). However, the developmental rate to the blastocyst stage was adversely affected by Cyst treatment (p < 0.05). The quality of embryos that reached the blastocyst stage, evaluated by total, inner cell mass (ICM) and trophectoderm cell numbers and ICM/total cell ratio was unaffected (p > 0.05) by treatments. The results indicate that ROS play a role in the fertilizing capacity in bovine spermatozoa, as well as in the interaction between the spermatozoa and the oocytes. It can be concluded that supplementation with antioxidants during IVF procedures impairs sperm quality, normal pronuclear formation and embryo development to the blastocyst stage.