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991.
992.
Some pharmacokinetic indices of oral fluconazole administration to koalas (Phascolarctos cinereus) infected with cryptococcosis 下载免费PDF全文
M. Govendir L. A. Black S. E. Jobbins B. Kimble R. Malik M. B. Krockenberger 《Journal of veterinary pharmacology and therapeutics》2016,39(4):412-415
Three asymptomatic koalas serologically positive for cryptococcosis and two symptomatic koalas were treated with 10 mg/kg fluconazole orally, twice daily for at least 2 weeks. The median plasma Cmax and AUC0‐8 h for asymptomatic animals were 0.9 μg/mL and 4.9 μg/mL·h, respectively; and for symptomatic animals 3.2 μg/mL and 17.3 μg/mL·h, respectively. An additional symptomatic koala was treated with fluconazole (10 mg/kg twice daily) and a subcutaneous amphotericin B infusion twice weekly. After 2 weeks the fluconazole Cmax was 3.7 μg/mL and the AUC0‐8 h was 25.8 μg/mL*h. An additional three koalas were treated with fluconazole 15 mg/kg twice daily for at least 2 weeks, with the same subcutaneous amphotericin protocol co‐administered to two of these koalas (Cmax: 5.0 μg/mL; mean AUC0‐8 h: 18.1 μg/mL*h). For all koalas, the fluconazole plasma Cmax failed to reach the MIC90 (16 μg/mL) to inhibit C. gattii. Fluconazole administered orally at either 10 or 15 mg/kg twice daily in conjunction with amphotericin is unlikely to attain therapeutic plasma concentrations. Suggestions to improve treatment of systemic cryptococcosis include testing pathogen susceptibility to fluconazole, monitoring plasma fluconazole concentrations, and administration of 20–25 mg/kg fluconazole orally, twice daily, with an amphotericin subcutaneous infusion twice weekly. 相似文献
993.
994.
The fungal genera associated with larvae ofStromatium fulvum were isolated externally, internally and also those from larval galleries and exit holes. They belonged to six genera:Penicillium, Trichoderma, Helminthosporium, Aspergillus, Rhizopus andChaetomium. Based on the antagonistic effect,Trichoderma andChaetomium were used in the mortality studies. Isolation of fungi from inoculated dead larvae proved thatCheatomium spp. are non pathogenic. The genera ofTrichoderma spp.,Aspergillus spp. andPenicillium spp. were pathogenic and are recommended as biological control agents forStromatium fulvum larvae. 相似文献
995.
A paper published byKniehase & Zoebelein (1990) describes a new laboratory method to test the side effects of pesticides on the predatory mitePhytoseiulus persimilis and criticizes the laboratory test developed by the International Working Group pesticides and beneficial organisms of the International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS). The present publication discusses the advantages and disadvantages of both methods, responds to the criticism and mention the overall concept of the IOBC/WPRS Working Group including semifield and field test methods.
Zusammenfassung In einer Arbeit vonKniehase undZoebelein (1990) wird ein neues Laborverfahren zur Prüfung der Nebenwirkung von Pflanzenschutzmitteln auf die RaubmilbePhytoseiulus persimilis beschrieben. Die genannten Autoren äußern sich kritisch über das seither von der Arbeitsgruppe Pflanzenschutzmittel und Nutzorganismen der International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS) praktizierte Verfahren. In der vorliegenden Arbeit werden die Vor- und Nachteile der beiden Prüfmethoden diskutiert. Einer Reaktion auf die Kritik folgt eine kurze Beschreibung der gesamten Konzeption der genannten Arbeitsgruppe, die auf einer Kombination aus Labor-, Halbfreiland- und Feldprüfverfahren beruht.相似文献
996.
Asma Hassan Shahzada Sohail Ijaz Rattan Lal Safdar Ali Qaiser Hussain Muhammad Ansar Romaan Hayat Khattak Muhammad Sharif Baloch 《Land Degradation \u0026amp; Development》2016,27(4):1175-1185
Depth distribution of soil organic carbon (SOC) fractions depends on the efficiency of agro‐technical managements. Information on depth distribution of SOC fractions mostly confined to the plow layer and scant in dry lands of Punjab, Pakistan. Therefore, a field experiment was laid out with moldboard plow (MP) (control), tine cultivator (TC), and minimum tillage (MT) as main plots, and cropping sequences fallow wheat (Triticum aestivum L.), (FW, control), mungbean (Vigna radiata L.) wheat (MW), sorghum (Sorghum bicolor L.) wheat (SW), green manure wheat (GW), and mungbean‐chickpea (MC) (Cicer arietinum L.) as sub‐plots. Treatment effects were assessed for microbial biomass carbon (MBC), potentially mineralizeable carbon (PMC), particulate organic carbon (POC), dissolved organic carbon (DOC), HCl insoluble carbon (HIC), and stratification ratio (SR) in Rawal series: Udic Haplustalf. Alfisols. The MBC concentration was the highest in MT system, at 15 to 30‐cm depth under MW and PMC concentration was highest under SW with MT at 45–60 cm. MP had higher POC in FW sequence. The highest DOC was at 0 to 15‐cm depth under MC with TC and stock of HIC was more under TC with FW sequence. The highest SR of PMC was under MT with FW at 0–15:15–30 and POC was under TC and MP with FW at depths of 0–15:45–60 cm. The highest SR for DOC was under MP with GW at 0–15:45–60 cm and HCl insoluble C was under MT with SW at 0–15:45–60. In broad‐spectrum, labile organic fractions revealed differential sensitivity, and POC stocks are also a sensitive indicator to detect the short‐management effects. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
997.
Temporal expression of transforming growth factor-beta2 and myostatin mRNA during embryonic myogenesis in Indian broilers 总被引:1,自引:0,他引:1
Saxena VK Sundaresan NR Malik F Ahmed KA Saxena M Kumar S Nandedkar PV Singh RV 《Research in veterinary science》2007,82(1):50-53
TGF-beta2 and myostatin, the members of TGF family, act through both autocrine and paracrine mechanisms to regulate the growth and differentiation at various developmental stages in chicken. The kinetics and expression profile of these two growth factors were investigated by semi-quantitative RT-PCR, during the myogenesis of Indian broiler chickens. Total RNA was isolated from whole embryos on each of embryonic days (E) 0-6 (n=3 per day) and from the biceps femoris muscle at E7-E18 (n=3 per day). The expression of TGF-beta2 was noticed on E2 that remained at the same level until E6. In biceps femoris muscle, higher level of TGF-beta2 expression was observed during E7-E12, which decreased gradually thereafter. These findings suggested that TGF-beta2 might be a regulatory factor participating in the myogenesis of chicken embryos. Initial myostatin expression was noticed on E1, even before the myogenic lineage is established in embryo. This finding suggested an additional role of myostatin in early chicken embryo development, other than myogenesis. Furthermore, myostatin expression was significantly higher on E3 as compared to earlier studies, where initial higher level was observed at E2, suggesting the differential expression of myostatin among breeds. Higher and almost static myostatin expression was noticed in biceps femoris muscle during the entire period of myogenesis (E7-E18). In the present study, the ontogeny of myostatin expression coincided with myogenesis of chicken. Therefore, it may be hypothesized that myostatin is not only a major determinant of muscle mass, but also involved in early embryogenesis in chickens. 相似文献
998.
Primate genomes contain a large number of endogenous retroviruses and encode evolutionarily dynamic proteins that provide intrinsic immunity to retroviral infections. We report here the resurrection of the core protein of a 4-million-year-old endogenous virus from the chimpanzee genome and show that the human variant of the intrinsic immune protein TRIM5alpha can actively prevent infection by this virus. However, we suggest that selective changes that have occurred in the human lineage during the acquisition of resistance to this virus, and perhaps similar viruses, may have left our species more susceptible to infection by human immunodeficiency virus type 1 (HIV-1). 相似文献
999.
1000.
Norris JM Bell ET Hales L Toribio JA White JD Wigney DI Baral RM Malik R 《Journal of Feline Medicine and Surgery》2007,9(4):300-308
Serum samples from 340 pet cats presented to three inner city clinics in Sydney Australia, 68 feral cats from two separate colonies in Sydney, and 329 cattery-confined pedigree and domestic cats in eastern Australia, were collected over a 2-year period and tested for antibodies directed against feline immunodeficiency virus (FIV) using immunomigration (Agen FIV Rapid Immunomigration test) and enzyme-linked immunosorbent assay methods (Snap Combo feline leukaemia virus antigen/FIV antibody test kit, IDEXX Laboratories). Western blot analysis was performed on samples in which there was discrepancy between the results. Information regarding breed, age, gender, housing arrangement and health status were recorded for all pet and cattery-confined cats, while the estimated age and current physical condition were recorded for feral cats. The FIV prevalence in the two feral cat populations was 21% and 25%. The majority of FIV-positive cats were male (60-80%). The FIV prevalence in cattery-confined cats was nil. The prevalence of FIV in the pet cat sample population was 8% (27/340) with almost equal prevalence in 'healthy' (13/170) and 'systemically unwell' (14/170) cats. The age of FIV-positive pet cats ranged from 3 to 19 years; all FIV-positive cats were domestic shorthairs with outside access. The median age of FIV-positive pet cats (11 years) was significantly greater than the median age of FIV-negative pet cats (7.5 years: P<0.05). The prevalence of FIV infection in male pet cats (21/172; 12%) was three times that in female pet cats (6/168; 4%; P<0.05). With over 80% of this pet cat population given outside access and continued FIV infection present in the feral population, this study highlights the need to develop rapid, accurate and cost-effective diagnostic methods that are not subject to false positives created by concurrent vaccination against FIV. This is especially important in re-homing stray cats within animal shelters and monitoring the efficacy of the new vaccine, which has not been challenged against Australian strains. The absence of FIV within cattery-confined cats highlights the value in routine screening and indoor lifestyles. This study provides cogent baseline FIV prevalences in three cat subpopulations which can be used for appraising potential disease associations with FIV in Australia. 相似文献