首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   536篇
  免费   14篇
林业   58篇
农学   25篇
基础科学   1篇
  97篇
综合类   33篇
农作物   13篇
水产渔业   44篇
畜牧兽医   220篇
园艺   13篇
植物保护   46篇
  2022年   2篇
  2021年   11篇
  2020年   7篇
  2019年   7篇
  2018年   8篇
  2017年   8篇
  2016年   16篇
  2015年   9篇
  2014年   13篇
  2013年   44篇
  2012年   31篇
  2011年   27篇
  2010年   15篇
  2009年   17篇
  2008年   28篇
  2007年   33篇
  2006年   23篇
  2005年   28篇
  2004年   26篇
  2003年   29篇
  2002年   20篇
  2001年   22篇
  2000年   26篇
  1999年   19篇
  1998年   6篇
  1997年   6篇
  1996年   7篇
  1995年   5篇
  1994年   1篇
  1992年   4篇
  1991年   5篇
  1990年   9篇
  1989年   5篇
  1988年   3篇
  1987年   3篇
  1986年   2篇
  1985年   5篇
  1984年   4篇
  1983年   3篇
  1981年   1篇
  1980年   2篇
  1978年   1篇
  1977年   1篇
  1976年   1篇
  1975年   1篇
  1972年   1篇
  1971年   1篇
  1970年   1篇
  1969年   1篇
  1964年   1篇
排序方式: 共有550条查询结果,搜索用时 15 毫秒
81.
The decline of virgin fir (Abies firma) forest at Mt. Oyama has been reported. Related field observations suggest that high acidity fog is linked with its decline. However, cedar (Cryptomeria japonica) in the same area shows no symptoms of decline. For assessing effects of acid fog on membrane-bound calcium (mCa) of the leaf mesophyll cells, 9-year-old seedlings of fir (Abies firma) and 8-year-old seedlings of cedar (Cryptomeria japonica) were exposed twice a week to simulated acid fog (SAF at pH 3 with pH 5 as control) for 2 h per day in a chamber during May–December 2007 (except August). Current and 1-year-old needles were collected from seedlings and analyzed at 1-month intervals. For current-year needles of fir, mCa levels in cells exposed to SAF at pH 3 were significantly lower than in cells exposed to pH 5, especially during September 2007–March 2008. In contrast, it is noteworthy that mCa levels of cedar were maintained as virtually constant irrespective of SAF acidity, indicating that fir is more sensitive to acid fog than is cedar. Based on these results, mCa loss by acid fog might also be caused in the declining virgin fir forest at Mt. Oyama.  相似文献   
82.
K. Miura    J. Wu    H. Sunohara    X. Wu    T. Matsumoto    M. Matsuoka    M. Ashikari    H. Kitano 《Plant Breeding》2009,128(1):63-69
Dwarf or semidwarf characters are an important trait for crop breeding as they provide lodging resistance. The rice mutant line DMF-1 has a dm-type semidwarf phenotype and high-lodging resistance controlled by the dominant gene, Short second internode 1 ( Ssi1 ). To elucidate the mechanism of reducing culm length in DMF-1, we sought to identify the Ssi1 gene by positional cloning using the chromosome segment substitution line as a crossing parent. As a result of high-resolution mapping, we found a 1.3-Mbp genomic inversion and a newly arranged gene in the Ssi1 locus. In this study, we report the high-resolution mapping and physical mapping of Ssi1 . We also discuss the possible function of a novel rearranged Ssi1 gene for the dominant dm-type semidwarf phenotype.  相似文献   
83.
The behavior of bigeye tuna (Thunnus obesus) in the northwestern Pacific Ocean was investigated using archival tag data for 28 fish [49–72 cm fork length (FL) at release, 3–503 days] released in Japanese waters around the Nansei Islands (24–29°N, 122–132°E) and east of central Honshu (Offshore central Honshu, 32–36°N, 142–148°E). Vertical behavior was classified into three types based on past studies: ‘characteristic’ (non‐associative), ‘associative’ (associated with floating objects) and ‘other’ (behavior not fitting into these two categories). The proportion of fish showing associative behavior decreased and that of characteristic behavior increased as fish grew, and this shift was pronounced at 60–70 cm FL. The fish usually stayed above the 20°C isotherm during the daytime and nighttime when showing associative behavior and below the 20°C isotherm during daytime for characteristic behavior. A higher proportion of characteristic behavior was seen between December and April around the Nansei Islands, and between September and December for offshore central Honshu. Seasonal changes in vertical position were also observed in conjunction with changes in water temperature. In this study, ‘other’ behavior was further classified into five types, of which ‘afternoon dive’ behavior, characterized by deep dives between around noon and evening, was the most frequent. The present study indicated that in the northwestern Pacific Ocean, the vertical behavior of bigeye tuna changes with size, as well as between seasons and regions.  相似文献   
84.
The rhythmic locomotor behavior of flies and mice provides a phenotype for the identification of clock genes, and the underlying molecular mechanism is well studied. However, interestingly, when examining locomotor rhythm in the wild, several key laboratory‐based assumptions on circadian behavior are not supported in natural conditions. The rooster crowing ‘cock‐a‐doodle‐doo’ is a symbol of the break of dawn in many countries. Previously, we used domestic inbred roosters and showed that the timing of roosters' crowing is regulated by the circadian clock under laboratory conditions. However, it is still unknown whether the regulation of crowing by circadian clock is observed under natural conditions. Therefore, here we used red jungle fowls and first confirmed that similar crowing rhythms with domesticated chickens are observed in red jungle fowls under the laboratory conditions. Red jungle fowls show predawn crowing before light onset under 12:12 light : dim light conditions and the free‐running rhythm of crowing under total dim light conditions. We next examined the crowing rhythms under semi‐wild conditions. Although the crowing of red jungle fowls changed seasonally under semi‐wild conditions, predawn crowing was observed before sunrise in all seasons. This evidence suggests that seasonally changed crowing of red jungle fowls is under the control of a circadian clock.  相似文献   
85.
Forests are one of the most important ecosystems on earth that require careful management, conservation, and sustainable exploitation. As countries have their own guideline systems, each may learn and borrow from one another’s experience. One of such countries is Japan, which has elaborate forest policies, and rich in forest cover (67%) with its forest history dating many years back. On the other hand, Kenya, with a forest cover of just 7% and its policies demonstrating notable weaknesses, has a lot to learn from Japan. Therefore, we have attempted to do a comparative analysis of forest policies, technologies, and management practices between Kenya and Japan. Results indicate that Kenya’s forest policies do not place adequate emphasis on silvicultural practices and the establishment of forest plantations, and rarely focus on sustainable biomass utilization—factors that contributed significantly to forest growth and development in Japan. Additionally, policy legislation, revision, and implementation have not been given the deserved priority in Kenya. We conclude that Kenyan forest policy would benefit from both revision and thorough implementation. We also discuss the role of indirect factors such as economic growth and availability of nonwood-based energy sources in the future of Kenya’s forests.  相似文献   
86.
Improved large ruminant productivity is increasingly acknowledged as a pathway for the alleviation of rural poverty and food insecurity in smallholder communities in Southeast Asia; yet, in much of Laos, bovine reproductive management is practically absent. Large ruminant reproduction skills were studied, using face-to-face surveys (n=60) of the knowledge, attitudes, and practices (KAP) of farmers, plus an extension of an examination of parameters of reproductive efficiency (n?=?1786 cattle and 434 buffalo) in the northern provinces of Luang Prabang and Xieng Khouang. The surveys particularly involved female farmers to provide gender-disaggregated data, with females making up 38.3 % of participants. Results confirmed that KAPs of smallholder farmers on bovine reproductive management were low (34–46 %) with trends toward higher KAP scores in male survey respondents. Poor reproductive parameters were identified in both provinces, with low calving percentages of 54–75 and 45–54 % in cattle and buffalo groups, respectively, and prolonged inter-calving intervals of 14.1–19.8 and 26.0 months for the cattle and buffalo groups, respectively. Improving the reproductive efficiency of large ruminants in the northern upland regions would enable smallholder farmers to be more effectively engaged in the dramatic economic growth of the Southeast Asia region, although these findings indicate that intensive training and supportive interventions are required to improve large ruminant reproductive outcomes in communities that have low-level large ruminant husbandry skills.  相似文献   
87.
Urbanisation is increasing tremendously in some parts of the world. Consequently, many rural forests may become depleted, although many opportunities exist for urban forests to increase. However, few studies have quantified the carbon (C) sequestration capacities of urban and rural forests in specific climatic zones. The present study compared carbon sequestration in two temperate deciduous forests located in Nagoya and Toyota, central Japan. The Nagoya University forest represented an urban forest, and a site in Toyota represented a rural forest. The urban forest at Nagoya University had comparatively smaller areas of green space and larger areas of buildings and roads. Land uses for building and road, which are typical of urban areas, result in smaller diurnal temperature ranges but higher air temperature, vapour pressure deficit, and atmospheric carbon dioxide (CO2) concentration. The urban forest in this study exhibited higher gross ecosystem exchange (GEE), especially in the active growing season from May to September, suggesting the possible effect of CO2 fertilisation. However, higher air temperatures caused comparatively smaller net ecosystem exchange (NEE) because of higher ecosystem respiration (RE). Although both forests functioned as CO2 sinks at annual time scales, the rural Toyota forest site (5.43 t C ha–1 yr–1) had 36% higher net ecosystem production (NEP=–NEE; the negative sign indicates uptake by the forest ecosystem from the atmosphere) than that at the urban forest. The higher normalised respiration (i.e., RE/GPP ratio; GPP=–GEE where GPP represents gross primary production) at the Nagoya University forest might be attributable to factors associated with the degree of urbanisation. Thus, in temperate forests, factors associated with urbanisation may reduce the atmospheric carbon sink function by accelerating respiration. This is an issue of global interest, as many countries are experiencing rapid urbanisation.  相似文献   
88.
The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event.  相似文献   
89.
The aim of the present study was to clarify the overall efficiency of porcine somatic cell nuclear transfer (SCNT) by incorporating cryopreservation of the cloned embryos before transfer. The SCNT embryos reconstructed with preadipocytes and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation (2.7%, 17/635). Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer.  相似文献   
90.
We examined the effects of low-dose fish oil ingestion on hepatic lipid accumulation caused after high cholesterol feeding in C57BL/6J mice. The mice were fed purified experimental diets consisting of 20 energy % (en%) safflower oil (SO or SO/CH), 2 en% fish oil + 18 en% safflower oil (2FO or 2FO/CH), or 5 en% fish oil + 15 en% safflower oil (5FO or 5FO/CH) with or without 2 weight % (wt %) cholesterol for 8 weeks. Hepatic triglyceride and total cholesterol contents were significantly lower in groups that were fed diets containing fish oil and cholesterol than in those that were fed safflower oil and cholesterol. The hepatic mRNA levels of fatty acid synthase (FAS) were lower in groups fed cholesterol or fish oil. Fatty acid oxidation-related hepatic gene expressions were higher in fish oil-fed groups. Fecal cholesterol excretion was higher in all cholesterol-fed groups; cholesterol excretion was high in groups fed fish oil and cholesterol. These results suggest that low-dose fish oil diets improve lipid metabolism by modifying the expression of lipid metabolism-related genes in the liver and increasing fecal cholesterol excretion.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号