结缕草种子打破休眠过程呼吸途径的研究

采用呼吸抑制剂测定了结缕草休眠与打破休眠种子在发芽过程中的各个呼吸代谢途径－糖酵解呼吸途径（ＥＭＰ）、三羧酸循环呼吸途径（ＴＣＡ）、磷酸戊糖呼吸途径（ＰＰＰ）的呼吸速率及其占总呼吸速率的比例，结果表明，休眠种子的总呼吸速率显著低于打破休眠种子的总呼吸速率，造成这种差异的原因是磷酸戊糖途径及三羧酸循环途径的呼吸速率低，这是休眠结缕草种子不能发芽的原因之一。     

新疆乌鲁木齐市犬绦虫感染状况调查

为了解乌鲁木齐市的犬绦虫感染情况,对2012—2015年在乌鲁木齐市8个区(县)采集的17 710份散养犬粪便,采用直接涂片法和漂浮法进行检查,对绦虫卵进行计数,统计感染率。结果共检出阳性样品1 870份,感染率为10.56%;半农半牧型区县的犬绦虫感染情况较主城区区县严重;4年内各区县的犬绦虫感染率整体呈下降趋势。建议各区县落实"犬犬驱虫,月月投药"策略,加强对城市流浪犬的管理。     

丹麦的水貂养殖业

根据有关丹麦水貂养殖业发展的历史经验及近20年来的种貂存栏数据资料，对丹麦的水貂养殖业现状进行了分析，并对1985～2005年丹麦水貂农场数量和貂场种貂存栏数量进行了比较。通过对丹麦水貂养殖业的分析对迅速发展的中国水貂养殖业有借鉴意义。     

广西动物防疫物资信息管理系统的构建与应用

为实现广西动物防疫物资的信息化管理,适时掌握广西动物防疫物资的流转状况,广西壮族自治区动物疫病预防控制中心开发了“广西壮族自治区动物防疫物资信息管理系统”。该系统为B/S架构,采用JSP技术和MySQL数据库平台。该系统建立后,广西各级动物疫病预防控制机构均可通过互联网登录,及时存储和统计动物防疫物资信息数据,全程监控和实时了解物资流转情况。该系统为广西动物防疫物资的规范化管理提供了重要的技术支持,也为全区重大动物疫病防控提供了坚强的后勤保障。     

食源性寄生虫的风险评估

风险(risk)是由一种或多种危害因子而引发危害事件的一种可能性或概率。危害因子可能属于物理的、化学的、微生物的范畴,病原微生物中的细菌、病毒、寄生虫等都可成为引发危害事件的风险因子。评估某一因子是否对人或其他生物构成威胁以及所危害的程度显得至关重要。事实上,存在于环境中的危险因子及其多种可能的传播途径都会引发多种危害性事件。因此,要想充分了解并揭示由危害因子引发危害的概率,以及其随后对人类健康造成的危害并非易事。风险评估则是对不良结果或不期望事件发生几率进行描述及定量的系统过程,现已被视为一种用于揭示存在于环境中的各种危害及其对人类健康造成的影响的方法。论文主要对食源性寄生虫风险评估的方法进行全面阐述。     

The effects of replacement of antibiotics with by‐products of oriental medicinal plants on growth performance and meat qualities in fattening pigs

The effect of by‐products of oriental medicinal plants (OMP; T1) containing 0.03% herb extracts (T2) or 0.1% aminolevulinic acid (T3) on the production performance of swine during the finishing period and on its meat quality were investigated. No significant differences were found in the weight gain, feed intake and feed conversion rate among the tested groups (P > 0.05). But the treated group showed higher (P < 0.05) moisture and ash and lower protein than the control group. The T3 group showed a lower meat cholesterol content (38.42 mg/100 g) compared to the other groups (P < 0.05). The vitamin E content of the muscle in the treated groups was higher compared to the control group. No antibiotic content was detected in all treated and control samples. The values of the volatile basic nitrogen (VBN) and thiobarbituric acid reactive substance (TBARS) of the treated groups were significantly lower (P < 0.01) than the control group. The treated groups had significantly better (P < 0.05) sensory‐test scores for color, flavor, off‐flavor and total acceptability compared to the control group.     

猪瘟病毒E2蛋白与猪RACK1相互作用的鉴定

为筛选和鉴定与猪瘟病毒(CSFV)E2蛋白相互作用的猪宿主蛋白,我们采用酵母双杂交方法筛选猪肺泡巨噬细胞表达文库得到与CSFV E2蛋白相互作用的宿主细胞RACK1蛋白,经共转化试验和GST pull-down试验进一步证实两者可以特异性结合,并且共聚焦试验表明两者共定位于细胞的细胞浆。本研究显示E2蛋白与宿主细胞RACK1蛋白存在相互作用,RACK1在CSFV感染过程中所发挥的功能有待进一步研究。     

Expression of 14-3-3 σ protein in normal and neoplastic canine mammary gland

14-3-3 σ protein is a negative cell cycle regulator, with both reduced and elevated levels associated with cancer in humans. This study assessed the expression of this protein in canine mammary tissues using immunohistochemistry and Western blotting. 14-3-3 σ was detected in 97% of the mammary tissue samples examined and was found in both myoepithelial (MECs) and epithelial (ECs) cells. Expression levels were elevated and reduced in neoplastic ECs and MECs, respectively (P < 0.001). Intense expression of 14-3-3 σ was detected in neoplastic ECs infiltrating blood vessels and lymph nodes and suggests a possible role for this protein in the malignant transformation of mammary neoplasms. Moreover, double immunostaining for 14-3-3 σ and the MEC – specific marker p63, confirmed that 14-3-3 σ is a highly sensitive marker of MECs since all p63 – positive cells were also positive for 14-3-3 σ. However, this protein is not exclusive to MECs as ECs also labelled positively.     

Effects of surgical implantation of temperature dataloggers on reproduction of captive striped skunks (Mephitis mephitis).

A total of 20 (14 females, six males) captive striped skunks (Mephitis mephitis) with miniature temperature dataloggers implanted free in the abdominal cavity were examined for reproductive performance and pathology because of implants. Eleven of 12 female skunks reproduced successfully 45.9+/-3.7 days after surgery to remove implanted dataloggers. The pregnancy rate of 91.7% (11/12) was much higher than other captive studies and was comparable with that of wild skunks. Eight striped skunks (six males, two females) that were euthanatized and necropsied after having implants in the abdominal cavity for 5 mo showed no apparent pathology associated with the implant. Implantation of the intraperitoneal devices did not lead to complications in abdominal tissues. Neither implant nor surgery affected reproduction. We conclude that implanted dataloggers can safely be used in physiologic studies of striped skunks or possibly other small carnivores in captive or field studies.     

利用太阳能烘干蚕茧的初步研究

以稀土生态农用膜为光能接受和转化物,将光能中的紫外线等转化为远红外线从而可利用太阳能烘干蚕茧。采用该方法在蚕茧适干时的温度比传统的茧灶干茧法稍低,且外界温度的高低直接影响茧层内的温度高低,从而影响烘茧时间的长短。由于远红外线直接穿透茧层干燥蛹体,所以不损伤茧丝品质,干茧解舒率可提高4.46个百分点。