几种粮食皮壳提取物的抗氧化活性测定

用甲醇和石油醚提取几种粮食皮壳，以β-胡萝卜素漂白法测定提取物的抗氧化活性，并且比较它们的提取率和抗氧化指数的高低。     

基于稳定同位素的江苏近海金乌贼摄食生态研究

为进一步掌握江苏近海金乌贼(Sepia esculenta)种群生活史特征，根据在江苏沿海港口所采集的样本，利用碳氮稳定同位素分析法对金乌贼的摄食生态及营养生态位进行了研究。结果表明，金乌贼雌、雄群体间 13C和 15N值均不存在显著性差异；金乌贼的 值与胴长之间相关性不显著， 值与胴长之间存在显著相关性；雄性金乌贼营养生态位宽度（SEAc=0.57‰2）与雌性（SEAc=0.63‰2）无明显差异，雌性略大于雄性，且两者高度重叠（0.62）。同位素聚类分析显示，可将金乌贼个体分为75~114mm、115~184mm和185~204mm三个胴长组；185~204mm胴长组营养生态位宽度最大（SEAc=1.77‰2），75~114mm胴长组营养生态位宽度最小（SEAc=0.39‰2）；仅75~114mm和115~184mm胴长组间生态位重叠率为中度重叠（0.34），其余胴长组间生态位重叠率低。分析认为，金乌贼δ13C值的变化主要受同位素基线值和金乌贼洄游模式影响，而δ15N值的变化主要受金乌贼摄食作用影响；雌性和雄性金乌贼群体之间食物资源和栖息地利用高度相似；不同发育阶段的金乌贼在营养生态位上具有明显差异。     

自由基化学改性氧化石墨烯对亚甲基蓝的吸附性能

氧化石墨烯（Graphene Oxide,GO）拥有较大的比表面积和较高的稳定性，可用来吸附水体中的多种污染物，其潜在功能稳定性具有规模化应用前景。考虑到GO在高级氧化体系中结构和形貌会发生改变，探究了经UV/H2O2和UV/过硫酸盐(Persulfate, PS)产生强氧化性自由基的体系处理后所得GOs对亚甲基蓝(Methylene Blue, MB)的吸附性能。氧化性自由基体系光源为300 W中压汞灯，30 mL GO储备液(1 mg/mL)在2种氧化体系下反应1、2、4 h后制得GOs。实验考察了不同反应条件对GOs吸附动力学的影响，傅里叶变换红外光谱(FTIR)研究了GOs氧化前后表面官能团的变化。结果显示，经UV/H2O2, UV/PS氧化1 h后，GOs表面的含氧官能团数量开始明显减少；吸附动力学过程更符合准二级动力学模型（R2>0.999）；吸附热力学过程更符合Langmuir模型，MB饱和吸附量(经UV/H2O2和UV/PS氧化1、2、4 h后分别记G1-1, G1-2, G1-3, G2-1, G2-2, G2-3)依次为580.26, 591.80, 598.63, 521.77, 554.91, 568.00 mg/g。研究表明，GO可以快速吸附较大浓度范围内的MB，且对低浓度染料表现出更好的去除效果；GO对MB的吸附量随着pH值的增加逐渐增大，且在碱性条件下的增加得更明显；经氧化处理后，GOs的吸附性能随着氧化时间的增长，性能减弱，尤其在UV/PS体系中更为显著。     

广州古代名园海山仙馆造园艺术探析

通过历史文献研究和实地考察，分析广州古代名园海山仙馆的造园历史和构景要素，概述其造园思想、布局特点、建筑特色和植物配置特点。     

2.5%保得乳油对小麦蚜虫控制作用的研究

对不同剂量的2.5%保得乳油防治麦蚜的效果进行了评价。结果表明,2.5%保得乳油施用有效成分为7.50、6.25g/hm2时,其防效均极显著优于对照药剂处理的防效,持效期达7d以上,对小麦生长安全无害。     

青背竹芋的快速繁殖技术研究

以青背竹芋(Maranta arundinacea)的侧芽为外植体,通过组织培养手段进行快繁研究.结果表明:外植体消毒以先用75%的酒精杀菌5 s,再用0.1%氯化汞杀菌10 min为宜;最佳增殖培养基为MS+6-BA 6.0 mg/L+NAA 0.4 mg/L,最适生根培养基为1/2MS+NAA 2.0 mg/L.     

不同扦插密度与施肥模式对马铃薯微型薯产量的影响

以中薯2号的脱毒试管苗为试材,研究不同扦插密度与施肥模式对成都地区马铃薯微型薯产量的影响.结果表明:扦插密度为78株/盘(624株/ m2),其产量最高,平均每盘合格薯为77.2个,平均每盘薯重155.5g;在生根第1、2周喷施烟草复合肥70 g+尿素30 g+水15 kg,在第3、第4周喷施烟草复合肥100 g+水15 kg,在第5、6周喷施烟草复合肥60 g+磷酸二氢钾40 g+水15 kg,采用该施肥处理产量最高,其单薯重为1.93 g,每盘产薯重178.4 g.     

Change of reactive oxygen species production in co-culture model of rat astrocytes and motor neurons VSC 4.1

AIM: To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS: The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS: Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION: Compared to independent culture, ASC reduces the resting ROS production by co-cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.     

Soluble expression of a CXCL10-loop3-EGF fusion protein and its anti-tumor activity

AIM: To evaluate the implication of CXCL10-loop3-EGF fusion protein for the activities of targeting tumor and anti-angiopoiesis. METHODS: RT-PCR was preformed to amplify CXCL10 coding sequence from PBMC activated by IFN-γ. CXCL10-loop3-EGF fusion gene, which was conducted by Over-Lap Extention PCR, was hinged up with plasmid pTG19-T, transfected to E. coli DH5α and processed positive colony selection. After ligated with plasmid pET32a(+), recombinant CXCL10-loop3-EGF fusion gene was then transfected to E. coli Origami B (DE3) and induced to express its coding fusion protein his-CXCL10-loop3-EGF. The recombinant fusion protein CXCL10-loop3 -EGF was purified by His-bind affinity chromatograph, enterokinase cleavage, ultrafiltration and dislysis. The transwell chemotatic test and HUVEC angiopoiesis inhibition test were performed to determine the anti-tumor responses and anti-angiopoiesis activity of CXCL10-loop3-EGF fusion protein. RESULTS: CXCL10-loop3-EGF fusion protein was successfully constructed and confirmed by SDS-PAGE analysis and Western blotting. Significant PBMC chematatic activity and HUVEC anti-angiopoiesis activity were observed. CONCLUSION: CXCL10-loop3-EGF fusion protein, which has perfect anti-tumor activity, is successfully constructed.     

科尔沁沙地耐旱乡土灌木树种的选择及其生长比较

在干旱年份，从科尔沁沙地干燥沙丘选择特别耐旱的灌木树种进行育苗试验。通过对1、2年生苗木生长状况分析：叶底珠（Securinega suffruticosa）、鼠李（Rhamnus porflorus）、桃叶卫矛（Evonyms bungeanus）、驼绒蒿（Eurotia arborescens）和杠柳（Periplocasepium）等适应性强，1年生苗木高度为对照树种胡枝子的49．6％～101．8％，2年生苗木为对照树种胡枝子的89．0％～97．9％，可以进一步进行沙地造林试验；东北木蓼生长缓慢，不宜继续进行造林试验。