Detection of potato spindle tuber viroid by molecular hybridization and bioassay. A large-scale comparison

Summary To assess the reliability of dot-spot hybridization assay for the Potato Spindle Tuber Viroid, more than 1000 potato samples were cross-tested by biological and hybridization methods. Both methods gave similar results with a range of cultivars. The dot-spot hybridization test was reproducible with formaldehyde-denaturated cell-sap samples stored at room temperature for over 6 months. This finding means that samples can be transported from field stations to a diagnostic laboratory and allows for cross-checking of results between laboratories. The data suggest that dot-spot hybridization is suitable for breeding and quarantine purposes.     

水晶彩鲫、红鲫、锦鲤、荷包红鲤杂交子代的生长和体色研究

利用人工授精的方法,进行水晶彩鲫、红鲫、锦鲤和荷包红鲤的相互杂交试验,测定各个杂交组合子代的成活率、生长速度和体色分离比例。结果表明：4种鱼能够相互杂交受精,孵出鱼苗。孵化率锦鲤自交最低为46．4％,其它组合为70％～80％;杂交鱼苗经28d的人工饲养,水晶彩鲫与荷包红鲤、锦鲤的正、反杂交,同其它杂交组合比较有明显的差别,其生长速度慢,个体之间差异大,成活率低;杂交子代的体型分为3类：鲫鱼型、鲤鱼型和鲤鲫型。鳞片反光组织(虹彩细胞或鸟粪素细胞)为2类：完全型、缺失型。体色分离复杂多样,水晶彩鲫与红鲫杂交是水晶彩鲫,红鲫与锦鲤、荷包红鲤杂交是青灰色鲤鲫杂种,水晶彩鲫与锦鲤、荷包红鲤杂交都会出现水晶彩色和青灰色鲤鲫杂种。     

不同纬度太湖新银鱼的食性比较及其饵料生物学容量评价

比较分析不同纬度水体中太湖新银鱼Ｎｅｏｓａｌａｎｘ ｔａｉｈｕｅｎｓｉｓ Ｃｈｅｎ的食谱,喜食种类和食物组成的季节变化。同时对其饵料生物学容量参数进行了分析和评价。