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41.
High inclusion levels of dehulled lupin (Lupinus angustifolius) in salmonid diets significantly decrease growth rates. This may be caused by the high concentrations of non‐starch polysaccharides including oligosaccharide (OS) in lupin. The antinutritive effects of OS have not yet been fully investigated in fish. The objective of this study was to determine the effect of enzyme supplementation of dehulled lupin‐based diets on the fish performance. There were two control diets: a fish meal‐based diet with no plant protein (FM) and a diet that contained 50% dehulled lupin (L). Four experimental diets based on diet L and containing four different exogenous enzyme supplements were used: diet L(E) (Energex™); diet L(B) (Bio‐Feed™ Pro); diet L(α) (Alpha galactosidase™); and diet L(Mix), which contained all the enzymes. Fish were randomly stocked into tanks in duplicate groups of 38 fish, 16.58±0.169 (SE) g, and were fed twice a day for 6 weeks. The supplemented enzymes did not improve weight gain in fish fed lupin‐based diets. However, mixed enzyme significantly improved Protein Efficiency Ratio (PER). Apparent digestibility of DM, CP and GE significantly improved in fish‐fed L(E) diet. None of the supplemented enzymes affected digestive tract indices or carcass composition. Surprisingly, weight gain was significantly higher in fish‐fed L(α), L(E) and L(Mix) diets as compared with FM diet. Feed intake was significantly higher in fish‐fed L, L(α) and L(E) diets compared with the FM diet. It is concluded that storing of lupin kernel under a suitable condition may have partially hidden the positive effects of exogenous enzymes through activating the endogenous enzymes. 相似文献
42.
Mehrdad Madani Juan E. Palomares-Rius Nicola Vovlas Pablo Castillo Mario Tenuta 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):1023-1039
Cyst nematodes obtained from commercial carrot fields in Ontario (Canada) and northern and southern Italy were subjected to morphological and molecular examination. Morphology of cyst cone tops, males and second-stage juveniles (J2) indicated the nematode species was the Carrot Cyst Nematode (CaCN), Heterodera carotae. The sequence of the Internal Transcribed Spacer (ITS), D2-D3 region of the 28S gene of ribosomal RNA, cytochrome oxidase I of mitochondrial DNA (coxI), and a heat shock protein gene (hsp90), from single cysts were also examined. Sequences of ITS and D2-D3 placed all the nematodes with Heterodera carotae and other Heterodera spp. belonging to the Goettingiana group in the same clade. The novel nine coxI sequences obtained also clustered in a well-supported phylogenetic clade for H. carotae. Similarly, the six new hsp90 sequences of H. carotae generated in this study were placed in a well-supported clade (PP = 1.00) together with other two sequences of H. carotae from Greece. Restriction Fragment Length Polymorphism (RFLP) of ITS-PCR products gave a restriction pattern for RsaI different than H. carotae but the other 6 restriction patterns were similar as described in former research. A diagnostic conventional PCR method was developed based on a primer set to be specific for H. carotae using coxI sequence. These primers were also used in real time PCR to generate a melt curve specific to H. carotae. Limit of detection for CaCN in conventional PCR reaction was a single J2. 相似文献
43.
A field experiment was carried out with a split plot arrangement based on a randomized complete block design with three replications in 2014. Triple super phosphate (0, 3.75, 7.5, 11.25 and 15 g m?2) was applied in main plots and phosphate bio-fertilizer (seed inoculation and topdressing) was allocated to sub plots. Results indicated that application of plants with phosphorus (P) bio-fertilizer as topdressing increased plant height and the number of the lateral branches under low levels of chemical P fertilizer, while plants from seed inoculated with P bio-fertilizer had more of those traits under high levels of chemical P fertilizer. Maximum leaf and flower dry weights were found under the lowest level of chemical P fertilizer. Moldavian balm plant demonstrated highly responsiveness to biological fertilizers in terms of increase in essential oil (EO) percentage and yield. It can be concluded that the effects of bio-fertilizer as seed inoculation is more during the early vegetative growth of plants. Thus, plants from seeds inoculated with P bio-fertilizer treatment had more biomass and EO percentage under high levels of chemical P fertilizer. 相似文献
44.
Sohrab Moradi Soleiman Mohammadi Limaei Peter Lohmander Mehrdad Khanmohammadi 《林业研究》2017,28(2):371-379
Although Zagros forests in western Iran lack industrial timber value due to the severe climate and socioeconomics problems, non-timber products are of great value and importance due to their high economic potential and also their potential to improve the welfare of forest-dwelling and forest-fringe villagers. This study was done in the forests of the Zemkan basin, an important part of central Zagros forests, Iran, to recognize the non-timber forest products (NTFPS), investigate their potential economic value and role in people’s livelihood. Data, collected using forest cruising, participatory observations, interviews with indigenous and local persons and experts, identified wild pistachio resin, wild pistachio fruit, oak fruit and oak syrup (Shokeh manna) among the non-timber products in this basin with total potential harvestable NTFPs of 77.16, 771.602, 13248.68, and 1324.868 tons per year, respectively. The economic rent from NTFPs is 33 US$/ha/year and its total expected values with consideration of real interest rate when the exploiter invests the capital in the bank (6.4 %) and when money is borrowed from the bank to execute the incorporated projects (8.4 %) are $516/ha and $393/ha, respectively. Furthermore, families’ economic share from potential profit of NTFPs is annually $601 per household. In addition wild pistachio resin has the highest share of the total potential profit of NTFPs and its equal to 51 % of the total of potential profit of NTFPs. Therefore, it is suggested that decisions be made to increase the infrastructure and strengthen the local selling market to enhance the cash income from NTFPs. Forest participatory management practices are suggested to organize and improve traditional use of the forests. 相似文献
45.
This study was conducted to investigate the physico-chemical properties and antioxidant activity of five pomegranate fruit (Punica granatum L.) cultivars grown in Iran. Significant differences were found among the pomegranate cultivars for many of the properties studied. Results showed that, in particular, fruit diameter ranged from 63.63 mm (Syah) to 79.29 mm (Rabab), fruit volume from 153.3 cm3 (Syah) to 293.3 cm3 (Rabab), and fruit density from 0.93 g cm?3 (Rabab) to 1.13 g cm?3 (TorshSefeed). Although Syah showed the lowest fruit weight (144.8 g), fruit yield (8.28 ton ha?1), and fruit skin thickness (1.55 mm), Rabab had the highest fruit yield (27.1 ton ha?1) and fruit skin thickness (2.32 mm). Juice volume was found to vary between 61.1 and 67.0 cm3. Percent of aril ranged from 59.64% (Rabab) to 75.3% (Syah) and weight of aril between 108.9 and 199.8 g. Also, results indicated that titratable acidity content varied from 0.39% (Syah) to 1.13% (TorshSefeed). The total soluble solids content varied from 12.67 ?Brix (TorshSefeed) to 15.67 ?Brix (ZardehAnar), pH values from 3.05 to 3.77, Electrical conductivity values from 2.8 to 3.14 dS m?1, and vitamin C content from 59.25 to 69.52 mg 100 g?1. The anthocyanin content was observed to vary between 80.36 (Syah) and 216.97 (ZardehAnar). The antioxidant activity of pomegranate cultivars ranged from 27.24% (Syah) to 84.04% (TorshSefeed). These results demonstrated that the cultivar was the major factor which influences the morpho-pomological and chemical (especially, antioxidant activity) properties of pomegranates. 相似文献
46.
Mehrdad Shamsaddini-Bafti Mahnaz Salehi Ali Maghsoudi Ali Mostafa Tehrani Farhad Mirzaei Syed Mojtaba Syed Momen 《畜牧与生物技术杂志(英文版)》2012,3(3):160-165
The aim of this study was to compare the quality characteristics and mineral content of the fiber from male and female cashmere goats raised under different management systems. Male and female Raeini cashmere goats (<1.5 years of age, n = 48) were selected from flocks raised at a government breeding station or raised commercially under either rural or nomadic conditions. The staple length, cashmere fiber diameter, coefficient of variation for fiber diameter, percentage of cashmere in a fleece, percentage of guard hair in a fleece and cashmere tenacity averaged 4.6 ±0.1 cm, 18.0 ±0.1 m, 20.9 ± 0.4%, 66.1 ± 1.5%, 33.8 ± 1.5% and 1.8 ± 0.2 gf/tex, respectively. The sulfur, copper and zinc content of the cashmere averaged 2.8 ± 0.1%, 0.00065 ± 0.00002% and 0.01276 ± 0.00025%, respectively. Rearing method significantly affected staple length, coefficient of variation of fiber diameter, cashmere tenacity and copper content. Males had a higher coefficient of variation of fiber diameter and cashmere tenacity than females (P < 0.05). 相似文献
47.
Talebkhan Garoussi M Farzaneh N Gallehdar E Mohri M 《Tropical animal health and production》2012,44(5):965-968
Pregnancy in out-of-breeding season in ewes increases the economical goals. Synchronization of estrus and ovulation is essential
for above program. The aims of this study using implant norgestomet with or without Pregnant Mare Serum Gonadotropin (PMSG)
were to evaluate the serum progesterone (P4) concentration changes: the conception rate and estimation of the lambing rate
and litter size. In total, 80 non-cycling multiparous Iranian Kurdish breed fat-tailed ewes with <0/5 ng/ml P4 were used in
April and May 2008 in the suburb of Mashhad, Iran. The animals were randomly divided into three groups: a control group (n = 30) without hormonal treatment, another group (n = 25) received 3 mg of norgestomet implant placed subcutaneously in the convex surface of the ear for 9 days, and the third
group (n = 25) treated with 3 mg of norgestomet implant for 9 days with an IM injection of 500 IU PMSG at implant removal. The progesterone
of treatment and control groups were measured on days 4, 9, and 13 after removal of the norgestomet using radioimmunoassay.
Every five ewes were exposed to one ram after 24 h of norgestomet removal in treatment and control groups, simultaneously.
The pregnancy was examined after 25 days of ram removal using ultrasonography. Progesterone concentration was significantly
higher in treatment groups on 9 and 13 days after norgestomet removal (P < 0.05). The pregnancy rate in the control, non-PMSG, and PMSG treatments groups were 17%, 52%, and 72%, respectively. The
rates of single and twin pregnancy in the non-PMSG treatment group were 69% and 31%, respectively. These rates in norgestomet
and PMSG treatment group were 50% and 39%, respectively. Triplet pregnancy (11%) was observed only in the PMSG treatment group.
It was concluded that using implant norgestomet especially accompanied with PMSG can increase and improve the fertilization
rate of ewe in the out-of-breeding season program. 相似文献
48.
Goli M Ezzatpanah H Ghavami M Chamani M Aminafshar M Toghiani M Eghbalsaied S 《Tropical animal health and production》2012,44(7):1673-1680
The major pathogens causing mastitis were evaluated by multiplex-polymerase chain reaction (M-PCR) with self-designed primers in four quarters of the first, third, and fifth parities in industrial, semi-industrial, and traditional dairy cattle farms in Iran. With the incidence of infection in the quarters by Staphylococcus aureus and Streptococcus agalactiae, the mean log somatic cell count (log SCC) increased from 5.06 to 5.77. The smallest changes occurred with Escherichia coli. Contagious pathogens, when compared with environmental pathogens, were more prevalent and common and created more profound quantitative and qualitative changes in SCC profiles. The second part of the study surveyed the diversity of contaminating pathogens and their effect on quantitative and qualitative profiles of somatic cells. M-PCR was used to determine the absence (M-PCR(-)) and presence of one (M-PCR(+1)), two (M-PCR(+2)), and three (M-PCR(+3)) major pathogens in raw milk samples. Quarter log SCC increased from 5.06 (for M-PCR(-1)) to 5.5 (for M-PCR(+1)), 5.7 (for M-PCR(+2)), and 6 (for M-PCR(+3)). Percent changes in polymorphonuclears (PMNs) were not significant between different quarters and parities but were significant between different farms in terms of pathogen diversity (P?0.05). Therefore, by increasing the number of types of major pathogens involved in subclinical mastitis, SCC of udder quarters and the proportion of PMNs significantly increased, whereas the proportion of lymphocytes significantly decreased. This subject is very important in increasing the shelf life of dairy products, because PMNs are introduced to the enzymatic pools. 相似文献
49.
50.
ABSTRACT The stem nematode Ditylenchus dipsaci is of great economic importance worldwide as a parasite of agricultural crops and horticultural plants. The internal transcribed spacer (ITS) of rDNA from 23 populations of the D. dipsaci complex from various host plants were amplified and sequenced. Seven previously studied populations were also included in the study. The phylogenetic analysis of the full ITS and ITS2 sequence alignments using minimum evolution, maximum parsimony, and Bayesian inference under the complex model of DNA evolution revealed trees with two main clades: (i) D. dipsaci sensu stricto with diploid chromosome numbers and comprising most isolates from agricultural, ornamental, and several wild plants, and (ii) Ditylenchus spp. with polyploid chromosome numbers, reproductively isolated from diploid populations, and subdivided into six subclades ("giant race" from Vicia faba, Ditylenchus species parasitizing various Asteraceae, and a Ditylenchus sp. from Plantago maritima). Using the energy minimization approach and comparative sequence analysis, it has been found that the secondary structure of ditylenchid ITS2 is organized in three main domains. The importance of knowledge on the RNA structure for phylogenetic analysis is discussed. Conventional polymerase chain reaction (PCR) and real-time PCR with SYBR green dye I with a species specific primer have been developed for detection and quantification of D. dipsaci sensu stricto Validation tests revealed a rather high correlation between real numbers of fourth-stage juveniles of the stem nematodes in a sample and expected numbers detected by real-time PCR. Problems of accuracy of quantification are discussed. 相似文献