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71.
Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease 下载免费PDF全文
E. Milne Y. Martinez Pereira C. Muir T. Scase D. J. Shaw G. McGregor L. Oldroyd E. Scurrell M. Martin C. Devine H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271
Objectives
To develop a provisional immunohistochemistry panel for distinguishing reactive pericardium, atypical mesothelial proliferation and mesothelioma in dogs.Materials and Methods
Archived pericardial biopsies were subject to haematoxylin and eosin staining, immunohistochemistry for cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Samples were scored for intensity and number of cells stained.Results
Ten biopsies of reactive mesothelium, 17 of atypical mesothelial proliferation, 26 of mesothelioma and five of normal pericardium were identified on the basis of haematoxylin and eosin staining. Cytokeratin and vimentin were expressed in all biopsies, confirming mesothelial origin. Normal pericardial samples had the lowest scores for insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Mesothelioma and atypical proliferative samples were similar to each other, with higher scores for insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 than the reactive samples. Desmin staining was variable. Insulin‐like growth factor II mRNA‐binding protein 3 was the best to distinguish between disease groups.Clinical Significance
An immunohistochemistry panel of cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 could provide superior information compared with haematoxylin and eosin staining alone in the diagnosis of cases of mesothelial proliferation in canine pericardium, but further validation is warranted. 相似文献72.
We investigated the soil and soil water chemistry in abandoned terraced paddy fields (reed stand) and a thicket of deciduous
broad-leaved trees (thicket stand) on the same slope in Sado Island, Japan. The soils gathered from these plots were incubated
under different water conditions to examine the dynamics of dissolved ions. The organic carbon pool in the soil in the reed
stand at the lower slope position was greater than the thicket stand at the middle slope position. The high concentration
of base cations and an almost neutral pH of the soil water at the reed stand corresponded with the high exchangeable cation
concentrations and base saturation in the soil. These results reflect the mineral-rich groundwater percolating down the slope,
which may be produced by chemical weathering. An in situ sulfate reduction in the reed stand at deeper soil horizons was identified.
The different water conditions in the incubated soils affected the soil pH(H2O), transformation of Fe, and dominant anions (NO3
−, HCO3
−, and SO4
2−). These biogeochemical processes were more conspicuous in the reed stand at the lower slope position where the concentrations
of organic matter and base cations were high. When the abandoned terraced paddy field is developed for the conservation of
the Japanese crested ibis (Nipponia nippon) habitat in Sado Island, the reductive subsoil at the lower slope position should be kept waterlogged to limit sulfuric acid
generation. 相似文献
73.
Takashi Naito Minoru Tanaka Satoshi Taba Tesuya Toyosato Atsushi Oshiro Kazuko Takaesu Kazuo Hokama Tomio Usugi Shinji Kawano 《Journal of General Plant Pathology》2007,73(2):139-141
In 1999, a disease of chrysanthemum [Dendranthema grandiflorum (Ramat.) Kitamura], characterized by virescence of flowers, occurred in Okinawa Prefecture. The causal agent was identified
as “Candidatus Phytoplasma aurantifolia” based on 16S rDNA sequencing.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB247462. 相似文献
74.
Breakdown and macroinvertebrate colonization of conifer needles (Cryptomeria japonica) and deciduous broadleaves (Euptelea polyandra) were investigated using litter bags in two study sites in streams flowing through a conifer plantation of C. japonica in Shikoku, southwestern Japan (one site with conifer canopy and another with mixed conifer and broadleaved canopy). Breakdown
rates and macroinvertebrate densities were compared between litter species (conifer needle vs broadleaf) and between the two
sites (conifer vs mixed canopy) to determine (1) whether breakdown rate of broadleaves is higher than conifer needles, (2)
whether macroinvertebrates prefer broadleaves to conifer needles, and (3) whether the difference in riparian canopy is reflected
in macroinvertebrate abundance. The results indicated that breakdown rates of broadleaves were higher than those of conifer
needles, suggesting poorer quality of the latter as food for macroinvertebrates. Differences in macroinvertebrate density
between needles and broadleaves were generally consistent with those in breakdown rates: broadleaves tended to have higher
densities than needles, suggesting that conifer needles were not preferred by macroinvertebrates. However, total macroinvertebrate
density in the conifer site was not significantly different from that in the mixed site, although the dominant shredder taxon
differed (conifer site: gammarids; mixed site: lepidostomatids). Although conifer needles are low-quality food for macroinvertebrates,
this may offer some advantages. Conifer needles remain on the streambed for longer periods owing to their lower breakdown
rates, being a constantly available resource. In addition, accumulations of conifer litter may effectively trap and retain
particulate organic matter. 相似文献
75.
Reto M Figueira ME Filipe HM Almeida CM 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(4):139-144
To evaluate the potential benefits and risks associated with tea consumption it is important to identify the constituents
of this beverage. Levels of some minerals, caffeine and catechins in green tea samples commercialized in Portugal were evaluated.
Potassium is the metal present in larger amount (92–151 mg/l). The content of sodium, calcium, fluoride, aluminium, manganese
and iron were 35–69, 1.9–3.5, 0.80–2.0, 1.0–2.2, 0.52–1.9, 0.020–0.128 mg/l, respectively. Chromium and selenium were not
detected. The resulting data showed considerable variability in catechins content. The levels of epigallocatechin gallate
(EGCG) ranged from 117 to 442 mg/l, epicatechin 3-gallate (EGC) from 203 to 471 mg/l, epigallocatechin (ECG) from 16.9 to
150 mg/l, epicatechin (EC) from 25 to 81 mg/l and catechin (C) from 9.03 to 115 mg/l. Caffeine contents in the green tea infusions
studied were between 141–338 mg/l. Green tea infusions provide significant amounts of catechins and could be an important
source of some minerals. 相似文献
76.
Growth,P uptake and rhizosphere properties of wheat and canola genotypes in an alkaline soil with low P availability 总被引:1,自引:0,他引:1
Zakaria Solaiman Petra Marschner Dongmei Wang Zed Rengel 《Biology and Fertility of Soils》2007,44(1):143-153
The aim of the present study was to assess the role of soil type on growth, P uptake and rhizosphere properties of wheat and
canola genotypes in an alkaline soil with low P availability. Two wheat (Goldmark and Janz) and two canola genotypes (Drum
and Outback) were grown in a calcareous soil (pH 8.5) at two P levels [no P addition (0P) or addition of 200 mg kg−1 P as Ca3(PO4)2 (200P)] and harvested at flowering or maturity. Shoot and root dry weight, root length and shoot P content were greater in
the two canola genotypes than in wheat. There were no consistent differences in available P, microbial P and phosphatase activity
in the rhizosphere of the different genotypes. Shoot P content was significantly positively correlated with root length, pH
and phosphatase activity in the rhizosphere. The microbial community composition, assessed by fatty acid methylester analysis,
of the canola genotypes differed strongly from that of the wheat genotypes. The weight percentage bacterial fatty acids, the
bacteria/fungi (b/f) ratio and the diversity of fatty acids were greater in the rhizosphere of the canolas than in the rhizosphere of the wheat
genotypes. In contrast to the earlier studies in an acidic soil, only small differences in growth and P uptake between the
genotypes of one crop were detected in the alkaline soil used here. The results confirmed the importance of root length for
P uptake in soils with low P availability and suggest that the rhizosphere microbial community composition may play a role
in the better growth of the canola compared to the wheat genotypes. 相似文献
77.
Edak Aniedi Uyoh Ikootobong Sunday Urua Valentine Otang Ntui Elza Cletus Okpako 《Journal of Crop Science and Biotechnology》2011,14(4):227-232
Nuclear DNA of three accessions of Parkia biglobosa collected from three locations in northern Cross River State was investigated using a Patec PA II flow cytometer equipped
with an argon ion laser (488 nm), and pictures of mitotic chromosomes were taken using a digital micro-camera (Canon) placed
on the eye piece of a binocular microscope at 100X oil immersion. Metaphase chromosome counts of 2n = 22 for accessions A
and C and 2n = 24 for accession B, were obtained and through flow cytometry, the three accessions were confirmed to be diploids.
The nuclear DNA content and genome size for the accessions were 1.5085, 1.489, and 1.5266 pg (737.7054, 728.121, and 746.5074
Mbp) for accessions A, B, and C, respectively. In another experiment, leaf samples from greenhouse-germinated seeds were analyzed
for variation in the banding pattern of the protein by SDS-PAGE in the three accessions. Protein was resolved into three banding
groups according to their electrophoretic mobility: slow, medium, and fast, clustering between 100–200, 40–70, and 10–25 kDa,
respectively. There was 76% similarity in the banding pattern between the accessions. 相似文献
78.
Canopy cover (CC) is a good predictor variable for plant growth parameters such as leaf area index and aboveground biomass.
A nondestructive, low-cost, and convenient method is presented for estimating CC using digital camera image analysis. CC was
estimated by the ratio of plant pixels to total pixels of digital camera image of rice field. To determine the criteria for
segmenting the rice plant from variable soil background, three mosaic images for rice plant, flooded/bare soil, and algae-infested
background were prepared from digital camera images that were taken in various field conditions. An image analysis program
was developed in Visual Basic to extract red, green, and blue (RGB) features from the mosaic images, calculate RGB-based color
indices, and compute the minimum segmentation error for separating rice plant from background. When judged by the segmentation
error, modified excessive green index (MEGI) showed the highest potential for segmenting rice plant from flooded/bare soil
background, followed by normalized green (g) and excessive green index (EGI). At the threshold MEGI value of 0.03, the segmentation
error was the lowest as 0.13%. Any single index considered was not satisfactory in segmenting rice plant from algae-infested
background. However, a discriminant function of 1.2553EGI + 0.01735G − 0.01474B was successful in segmenting rice plant from
flooded/bare soil and algaeinfested background with segmentation errors of 0.34 and 1.17%, respectively. CC for four rice
varieties from tillering to booting stage was estimated based on the threshold value of MEGI and discriminant function and
also manually using commercial software. Both estimates of CC showed good relationship of r2 = 0.94, suggesting that a digital camera could be used efficiently for measuring the CC of rice field. 相似文献
79.
80.
Martin MW Grazhdankin DV Bowring SA Evans DA Fedonkin MA Kirschvink JL 《Science (New York, N.Y.)》2000,288(5467):841-845
A uranium-lead zircon age for a volcanic ash interstratified with fossil-bearing, shallow marine siliciclastic rocks in the Zimnie Gory section of the White Sea region indicates that a diverse assemblage of body and trace fossils occurred before 555.3 +/- 0.3 million years ago. This age is a minimum for the oldest well-documented triploblastic bilaterian Kimberella. It also makes co-occurring trace fossils the oldest that are reliably dated. This determination of age implies that there is no simple relation between Ediacaran diversity and the carbon isotopic composition of Neoproterozoic seawater. 相似文献